4.6 Article

Glycodelin suppresses endometrial cell migration and invasion but stimulates spheroid attachment

Journal

REPRODUCTIVE BIOMEDICINE ONLINE
Volume 24, Issue 6, Pages 639-645

Publisher

ELSEVIER SCI LTD
DOI: 10.1016/j.rbmo.2012.03.004

Keywords

attachment; glycodelin; invasion; migration; spheroid

Funding

  1. Committee on Research and Conference Grant
  2. University of Hong Kong
  3. Hong Kong Research Grant Council

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Glycodelin contains four isoforms with diverse biological functions. Glycodelin-A is potentially a diagnostic marker for cancer patients and receptivity marker of the secretory endometrium. Yet, direct evidence for the role of glycodelin in the regulation of endometrial epithelial cell migration, invasion and attachment of trophoblastic spheroids (blastocyst surrogate) is lacking. In this study, the human glycodelin gene was stably transfected into human endometrial (HEC1-B) cells. Forced expression of glycodelin in HEC1-B cells did not affect cell proliferation, cell viability or cell-cycle progression, but significantly reduced migration and invasion of the stably transfected cells (both P < 0.05). The migration rate returned to normal levels when the glycodelin-forced-expressing HEC1-B cells were treated with glycodelin RNAi. Furthermore, forced expression of glycodelin in HEC1-B cells significantly increased the attachment of trophoblastic spheroids onto the endometrial epithelial cells (P < 0.05). In summary, glycodelin suppressed endometrial cell migration and invasion but enhanced spheroid attachment. (C) 2012, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

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