Article
Biochemistry & Molecular Biology
Aisha Fahim, Onofrio Annunziata
Summary: The addition of small amounts of HEPES near physiological ionic strength is essential for converting protein-rich droplets into compact tetragonal crystalline microparticles, which exhibit small size, narrow size distribution, and guest-binding properties. The interactions between lysozyme and HEPES were found to be weakly attractive and exothermic. These findings suggest that salting-in type additives play an important role in controlling the fate of metastable protein-rich microdroplets.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Obstetrics & Gynecology
Amanda Souza Setti, Gabriela Halpern, Daniela Paes de Almeida Ferreira Braga, Assumpto Iaconelli Jr, Edson Borges Jr
Summary: Maternal lifestyle factors, such as smoking, alcohol consumption, refined sugar, and artificial sweeteners, have a significant impact on the incidence of oocyte dimorphisms and outcomes of intracytoplasmic sperm injection (ICSI) cycles. These habits are positively associated with oocyte dimorphisms and negatively associated with ovarian stimulation response, embryo development, clinical pregnancy rates, and live birth rates. The associations are dose-dependent.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Article
Agriculture, Dairy & Animal Science
Jana Rychtarova, Alena Langerova, Helena Fulka, Pasqualino Loi, Michal Benc, Josef Fulka
Summary: The long-term storage of biological material is crucial for various fields, and a simple approach for evaluating sperm DNA damage level has been proposed to address the issue of limited frozen biological material quantities.
Article
Chemistry, Multidisciplinary
Xiaoying Wang, Yusong Wang, Liping Yin, Qiang Zhang, Shaozhen Wang
Summary: By employing a surfactant-free synthesis strategy, water-soluble fluorescent nanoclusters were prepared for hypochlorous acid sensing and imaging in living cells.
Article
Medicine, General & Internal
Carlotta Scarafia, Martina Masciovecchio, Stefano Canosa, Andrea Roberto Carosso, Gianluca Gennarelli, Alberto Revelli, Chiara Benedetto
Summary: The study found that in women with ovarian endometriosis, ICSI can be performed without harming oocytes or affecting the outcome. Additionally, there is no advantage in removing endometrioma before ICSI.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Materials Science, Multidisciplinary
Weiming Ji, Shunni Dong, Wei Fan, Chao Lv, Jingjing Nie, Binyang Du
Summary: A functional monomer, 1-hexene-4-(benzal-2,2':6',2 ''-terpyridines)pyridinium bromide (HTP), was synthesized and incorporated into microgels, forming HTP-functionalized microgels (HTP-MGs). These HTP-MGs demonstrated selective, sensitive, and optical response to Fe2+ ions in aqueous solution, as well as potential applications in encryption and anti-counterfeiting.
ACS APPLIED POLYMER MATERIALS
(2021)
Article
Endocrinology & Metabolism
Camille Robin, Audrey Uk, Christine Decanter, Helene Behal, Pierre Collinet, Chrystele Rubod, Anne-Laure Barbotin, Geoffroy Robin
Summary: The study aimed to evaluate the relationship between endometriosis and oocyte morphology after controlled ovarian stimulation for intracytoplasmic sperm injection (ICSI) on a large oocyte cohort, finding that endometriosis does not have a negative impact on oocytes' morphology in IVF-ICSI.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Olga Tepla, Zinovij Topurko, Simona Jirsova, Martina Moosova, Eva Fajmonova, Radek Cabela, Katerina Komrskova, Irena Kratochvilova, Jaromir Masata
Summary: The aim of this study was to evaluate the efficiency of using meiotic spindle (MS) visibility and relative position to the polar body (PB) as indicators of oocyte maturation in order to optimize intracytoplasmic sperm injection (ICSI) timing. The visualization of MS influenced the pregnancy rate (PR) for patients over 35 years, but did not affect PR for patients under 35 years. Visualization of MS appears to be a useful parameter for assessment of oocyte maturity and ICSI timing for patients older than 35.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Shichao Wu, Shuo Hu, Xiangrui Yang
Summary: A novel strategy for the preparation of metal-organic particle-based nanomedicine, with high drug loading and good targeting properties, has been developed. The in vivo experiments showed significantly enhanced antitumor efficiency and minimal side effects.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Analytical
Alessio Incocciati, Lucia Bertuccini, Alberto Boffi, Alberto Macone, Alessandra Bonamore
Summary: Ferritins are ideal nanoparticles for drug delivery due to their hollow-sphere structure and ability to target cell surface receptors. Researchers have developed and characterized a new ferritin by modifying its surface residues, making it suitable for packaging small therapeutic molecules.
Article
Endocrinology & Metabolism
Xiaokun Hu, Yuliang Liu, Xiubing Zhang, Pingyin Lee, Yangxing Wen, Chenhui Ding, Canquan Zhou, Yanwen Xu
Summary: This study aimed to investigate the impact of oocyte degeneration after ICSI on cumulative live birth rate in young women; The results showed that oocyte degeneration is not an indicator for predicting the cumulative live birth rate.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Obstetrics & Gynecology
Takashi Morimoto, Tomohiro Maekawa, Shimpei Mizuta, Hidehiko Matsubayashi, Takumi Takeuchi, Yutaka Hata, Tomomoto Ishikawa
Summary: During the intracytoplasmic sperm injection (ICSI) procedure, unintentional membrane rupture (UMR) often leads to oocyte degeneration. The study showed that the ICSI Position Detector (IPD) can be helpful in identifying the best puncture location to prevent UMR and improve the success of the procedure.
REPRODUCTIVE BIOMEDICINE ONLINE
(2023)
Article
Reproductive Biology
G. Clerico, G. Taminelli, J. C. Veronesi, J. Polola, N. Pagura, C. Pinto, M. Sansinena
Summary: Oocyte vitrification in horses increases ROS levels, which can be mitigated by melatonin to improve mitochondrial function and ICSI embryo development. However, the impact of melatonin on pregnancy rates requires further investigation.
Article
Soil Science
Chongyang Li, Jordon Wade, Andrew J. Margenot
Summary: Buffers are widely used in soil enzyme assays to measure specific activities or pH optima, but the pH stabilization capacity of commonly used MUB buffer may deviate significantly in different soils, even with increased concentration. Forcing a specific pH in enzyme activity assays in soils appears difficult, presenting a tension between maintaining pH and maximizing assay sensitivity.
BIOLOGY AND FERTILITY OF SOILS
(2021)
Article
Andrology
Daniela Paes Almeida Ferreira Braga, Amanda Setti, Christina Morishima, Rodrigo R. Provenza, Assumpto Iaconelli Jr, Edson Borges Jr
Summary: This study aimed to investigate the relationship between sperm DNA fragmentation and intracytoplasmic sperm injection (ICSI) outcomes, and whether this relationship is influenced by the presence of oocyte dimorphisms. The results showed that ICSI cycles with lower sperm DNA fragmentation had higher rates of fertilization, high-quality embryos, implantation, and pregnancy. The presence of oocyte dimorphisms significantly affected the outcomes. The lowest rates of fertilization and high-quality embryos were observed when high sperm DNA fragmentation was associated with certain oocyte defects, such as dark cytoplasm, vacuoles, resistant membrane, and non-resistant membrane.
Article
Obstetrics & Gynecology
Alice Newman-Sanders, Jackson C. Kirkman-Brown, Meurig T. Gallagher
Summary: This study revealed a significant lack of awareness among young adults in the UK regarding the potential impacts of gym lifestyles and supplementation on male infertility. Men were found to have a concerning lack of concern for their own fertility, with differences in awareness levels between men and women. It was also observed that men were more likely to consider making changes to their behavior if it had a long-term impact on their fertility compared to short-term effects.
REPRODUCTIVE BIOMEDICINE ONLINE
(2024)
Article
Obstetrics & Gynecology
Shachar Reuvenny, Michal Youngster, Almog Luz, Rohi Hourvitz, Ettie Maman, Micha Baum, Ariel Hourvitz
Summary: Using a machine-learning model to determine the optimal trigger days can improve the outcomes of antagonist protocol cycles in freeze-all or fresh transfer cycles, for all age groups. Implementing these models can more accurately predict the number of retrieved oocytes, optimizing physicians' decisions, balancing workloads, and creating more standardized yet patient-specific protocols.
REPRODUCTIVE BIOMEDICINE ONLINE
(2024)
Article
Obstetrics & Gynecology
Chao Chen, Qi Wen, Feng Deng, Rong Li, Ying Wang, Xiumei Zhen, Jing Hang
Summary: This study investigates the proteomic and phosphoproteomic differences in the endometrium of women with recurrent pregnancy loss (RPL) compared to healthy control women during different phases of the menstrual cycle. The results identify differentially expressed proteins and phosphorylated proteins, and highlight the insulin/cyclic nucleotide signalling pathway and AMPK/mTOR signalling pathway as major contributors to the abnormality of RPL endometrium. The findings provide insights into potential proteins associated with the pathogenesis of RPL and contribute to the identification of potential targets for RPL treatment.
REPRODUCTIVE BIOMEDICINE ONLINE
(2024)
Article
Obstetrics & Gynecology
Jaime Guerrero, Juan Carlos Castillo, Jorge Ten, Jose Antonio Ortiz, Belen Lledo, Domingo Orozco, Francisco Quereda, Andrea Bernabeu, Rafael Bernabeu
Summary: The study found no significant differences in clinical outcomes between using oocytes obtained from random-start protocols and those from conventional ovarian stimulation in oocyte donation treatments. Luteal-phase stimulation required longer stimulation and higher FSH consumption.
REPRODUCTIVE BIOMEDICINE ONLINE
(2024)