Article
Biochemistry & Molecular Biology
Xianrong Xiong, Xiaojian Zhang, Manzhen Yang, Yanjin Zhu, Hailing Yu, Xixi Fei, Fuko Mastuda, Daoliang Lan, Yan Xiong, Wei Fu, Shi Yin, Jian Li
Summary: This study found that KDM2a is involved in follicular and oocyte development in mice, and its knockout leads to female subfertility. Additionally, KDM2a also affects the sensitivity to female hormones.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yingjun Chen, Qinghua Liu, Ruiyan Liu, Chan Yang, Xiaodong Wang, Zaohong Ran, Shanshan Zhou, Xiang Li, Changjiu He
Summary: This study in a mouse model revealed that early folliculogenesis requires a minimum of two weeks, with different stages showing specific characteristics such as oocyte size at Type 4-5 stage and theca folliculi formation at PD10-12. The study also highlighted the importance of the PD22-23 period for studying puberty initiation, providing valuable insights into the biological events of this process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agriculture, Dairy & Animal Science
Vanuzia Goncalves Menezes, Alane Pains Oliveira do Monte, Ricassio de Sousa Barberino, Bruna Bortoloni Gouveia, Thae Lane Barbosa Gama Lins, Tais Jobard Silva e Macedo, Joaozito Liandro de Oliveira, Cleyton de Almeida Araujo, Fleming Sena Campos, Emanuel Felipe de Oliveira, Andre Mariano Batista, Mario Adriano Avila Queiroz, Gherman Garcia Leal de Araujo, Maria Helena Tavares de Matos, Aurea Wischral
Summary: This study evaluated the effect of reduced water intake on survival, apoptosis and immunoexpression of leptin in sheep preantral follicles, activation of primordial follicles, serum levels of leptin, estradiol (E2) and progesterone (P4), and in vitro maturation (IVM) of oocytes antral follicles, as well evaluated the effects of leptin on in vitro culture of secondary follicles isolated these animals.
ANIMAL REPRODUCTION SCIENCE
(2023)
Article
Medicine, General & Internal
Wen-Bin Wu, Hsuan-Ting Chen, Jun-Jie Lin, Tsung-Hsuan Lai
Summary: The study revealed a negative association between follicular fluid VEGF concentration and ovarian reserve level, as well as oocyte maturation rate in patients undergoing GnRH antagonist IVF protocols. Additionally, a negative correlation was found between follicular fluid VEGF concentration and serum anti-Mullerian hormone (AMH) level. Oocyte maturation rate tended to increase at higher AMH levels.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Cell Biology
Chalikkaran Thilakan Rejani, Ajit Kumar Navin, Thekkey Madathil Valappil Mumthaz, Venugopal Bhuvarahamurthy
Summary: The impact of a high-fat-high-fructose diet on ovarian function is still unclear. This study found that this diet resulted in reduced autophagy in the ovaries, leading to abnormal follicle differentiation and impaired fertility.
Article
Reproductive Biology
Xiao Jian Lai, Shuai Peng, Yi Lei Wang
Summary: This study investigates the dynamic molecular regulation of follicular development in Anguilla japonica, focusing on the asynchronous development of follicles within the same individual. The findings suggest that steroid hormone synthesis and nutrient absorption play important roles in follicular development and maturation.
Review
Environmental Sciences
Keerthi Priya, Manjunath Setty, Uddagiri Venkanna Babu, Karkala Sreedhara Ranganath Pai
Summary: The pool of primordial follicles formed in the ovaries during early development impacts the fertility and quality of women's reproductive life, while occupational and environmental toxicants (ETs) have adverse effects on female fertility. Multiple factors may contribute to reduced fertility in women.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Pharmacology & Pharmacy
Xin-Ran Zhang, Ying-Chun Ouyang, Tie-Gang Meng, Hong-Yong Zhang, Wei Yue, Feng-Ze Yan, Yue Xue, Heide Schatten, Zhen-Bo Wang, Qing-Yuan Sun
Summary: This study evaluated the effects of OTSSP167 on reproductive capability in female mice and found that it can block the development of oocytes, leading to a decrease in their quantity and quality. Additionally, OTSSP167 can induce follicular atresia and decrease the number of follicles at different stages of development.
Article
Fisheries
Feiyan Meng, Shu Sun, Xing Xu, Weixing Yu, Riping Gan, Lihong Zhang, Weimin Zhang
Summary: The study investigates the regulation of oocyte growth and maturation in the ricefield eel, which has become an important aquaculture species in China. The researchers classified the development of ricefield eel ovarian follicles into nine stages and performed transcriptomic analysis on isolated ovarian follicles from different stages. The study identifies several genes and pathways that may be involved in oocyte growth and maturation in ricefield eels.
Article
Reproductive Biology
Juliana Z. Almeida, L. A. Vieira, C. Maside, A. C. A. Ferreira, N. A. R. Sa, H. H. Correia, V. R. Araujo, R. S. Raposo, J. Smitz, C. C. Campello, J. R. Figueiredo, R. B. Oria
Summary: 5FU does not affect follicular morphology but detrimentally affects follicular growth, estradiol production, and oocyte maturation in isolated ovarian follicles. Concentrations of 5FU greater than 3 mM reduce estradiol levels.
Review
Biochemistry & Molecular Biology
Karolina Wolodko, Juan Castillo-Fernandez, Gavin Kelsey, Antonio Galvao
Summary: Maternal obesity can lead to impaired leptin signaling, resulting in high levels of estrogen and leptin in the bloodstream, which adversely affects ovarian function. This review highlights the potential impact of impaired leptin signaling on folliculogenesis and oocyte developmental competence in both mice and women.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Obstetrics & Gynecology
Barbara Rodrigues Nascimento, Danielle Storino de Freitas, Julia Meireles Nogueira, Clara Carvalho e Souza, Rayan Silva de Paula, Jousie Michel Pereira, Ana Paula Madureira, Luciola Silva Barcelos, Erika Cristina Jorge, Paulo Henrique Almeida Campos-Junior
Summary: Ovarian cryopreservation is a viable option for fertility preservation and subcutaneous transplantation is a promising site. This study aimed to evaluate the effect of subcutaneous autotransplantation and vitrification on follicular growth and atresia, as well as their relationship with vascular perfusion and gene expression. The results showed that the first day after subcutaneous transplantation is critical for follicular survival, with intense follicular atresia independent of Bax upregulation.
REPRODUCTIVE SCIENCES
(2023)
Article
Reproductive Biology
C. B. Reed, S. Meier, L. A. Murray, C. R. Burke, J. L. Pitman
Summary: Our study compared the follicular microenvironment of heifers and cows with positive genetic merit for fertility, finding that cows with high genetic merit have higher quality cumulus cell-oocyte complexes and oocytes. Lower fertility value heifers had inconsistent sampling times, lower COC maturity, and differential gene expression associated with follicular maturation.
Review
Biochemistry & Molecular Biology
Eva Nagyova, Lucie Nemcova, Antonella Camaioni
Summary: The extracellular matrix plays a crucial role in the development of ovarian follicles, and its formation and function are regulated by proteolytic cleavage and hormonal stimulation. Specifically, the proteasome inhibitor MG132 affects the cytoskeletal structure, organization of cumulus ECM, steroidogenesis, and nuclear maturation in gonadotropin-stimulated oocyte-cumulus complexes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Reproductive Biology
Regina Lucia dos Santos Silva, Ricassio de Sousa Barberino, Maria Helena Tavares de Matose
Summary: The in vitro culture of ovarian preantral follicles allows studying follicular and oocyte growth, using immature oocytes as a potential source for fertilization and screening of ovarian toxic substances. However, oxidative stress caused by reactive oxygen species (ROS) accumulation poses a challenge to the success of the culture system. The addition of antioxidant agents to the culture medium can minimize the damage caused by ROS, supporting follicular survival and development, and producing mature oocytes suitable for fertilization.
Article
Cell Biology
D. C. C. Brito, L. V. S. Naupas, S. S. Souza, G. L. H. Alcantara, J. R. Figueiredo, R. R. Santos, A. P. R. Rodrigues
Summary: The type and period of fixation affected the percentage of morphologically normal ovarian follicles. Paraformaldehyde was not recommended as a fixative, while formalin was suitable for 12-hour fixation and Carnoy's solution was efficient for 24-hour fixation. Davidson's solution was the most suitable fixative for evaluating the morphology of ovarian preantral follicles, regardless of the fixation period.
Article
Cell Biology
Francisco Denilson Rodrigues Gomes, Danielle Cristina Calado de Brito, Naiza Arcangela Ribeiro de Sa, Lucy Vanessa Sulca Naupas, Gaby Judith Quispe Palomino, Renato Felix da Silva, Everton Pimentel Ferreira Lopes, Gildas Tetaping Mbemya, Benner Geraldo Alves, Mary Zelinski, Jose Ricardo de Figueiredo, Ana Paula Ribeiro Rodrigues
Summary: The study found that VSF can grow and develop during 12 days of in vitro culture, and showed evidence of preservation of steroidogenic function and remodeling of the basement membrane.
CELL AND TISSUE BANKING
(2022)
Article
Cell Biology
J. Cadenas, N. A. R. Sa, A. C. A. Ferreira, V. M. Paes, F. L. N. Aguiar, C. C. L. Fernandes, R. Rossetto, B. G. Alves, G. A. Apgar, D. M. Magalhaes-Padilha, D. Rondina, J. R. Figueiredo
Summary: This study evaluated the effects of whole flaxseed as a feed supplementation on the in vitro development of caprine early antral follicles and embryo production. The results showed that flaxseed did not affect follicular growth and diameter, but increased the recovery rate of oocytes and negatively affected sperm penetration rate.
Article
Reproductive Biology
Juliana Z. Almeida, L. A. Vieira, C. Maside, A. C. A. Ferreira, N. A. R. Sa, H. H. Correia, V. R. Araujo, R. S. Raposo, J. Smitz, C. C. Campello, J. R. Figueiredo, R. B. Oria
Summary: 5FU does not affect follicular morphology but detrimentally affects follicular growth, estradiol production, and oocyte maturation in isolated ovarian follicles. Concentrations of 5FU greater than 3 mM reduce estradiol levels.
Article
Reproductive Biology
A. D. Sales, A. B. G. Duarte, R. M. P. Rocha, I. R. Brito, Y. Locatelli, B. G. Alves, K. A. Alves, J. R. Figueiredo, A. P. R. Rodrigues
Summary: This study aimed to investigate the relative abundances of ABC transporters mRNA transcripts in ovine ovarian tissue after vitrification and in vitro culture. The results showed a negative correlation between degenerated follicles and mRNA transcript abundances of ABCB1 and ABCG2, with a significant decrease in the percentage of growing follicles after in vitro culture. Further studies are needed to explore the functions of ABC transporters in ovine folliculogenesis after in vitro culture.
Article
Cell Biology
D. C. C. Brito, I. P. Silva, A. C. A. Ferreira, N. A. R. Sa, M. I. F. Guedes, A. P. R. Rodrigues, R. R. Santos, J. R. Figueiredo
Summary: This study evaluated the effects of zearalenone and matairesinol on the morphology of in vitro-cultured ovarian follicles. Zearalenone impaired the follicle morphology, while the combination of matairesinol and zearalenone counteracted the negative effects. However, matairesinol did not promote follicle development in vitro.
Article
Reproductive Biology
A. C. A. Ferreira, N. A. R. Sa, R. F. Silva, F. G. C. Sousa, J. C. Anjos, J. Cadenas, B. G. Alves, L. F. Lima, J. J. H. Celestino, M. O. Gastal, A. P. R. Rodrigues, E. L. Gastal, J. R. Figueiredo
Summary: This study compared the morphological features, estradiol production, and gene expression between in vitro-derived and in vivo-derived early antral caprine follicles. The results showed that in vitro-derived antral follicles cultured in a specific medium had comparable oocyte maturation rates to in vivo-derived early antral follicles.
Article
Veterinary Sciences
R. F. Silva, L. F. Lima, Anna C. A. Ferreira, A. F. B. Silva, D. R. Alves, B. G. Alves, A. C. Oliveira, Selene M. Morais, Ana Paula R. Rodrigues, Regiane R. Santos, J. R. Figueiredo
Summary: This study evaluated the effects of different concentrations of eugenol, ascorbic acid, and anethole in goat ovarian tissue. The results showed that eugenol promoted follicle development and survival, and reduced oxidative stress. Furthermore, eugenol decreased gene methylation and endoplasmic reticulum stress.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Jose Renato S. Passos, Denise D. Guerreiro, Kamila S. Otavio, Pedro C. dos Santos-Neto, Marcela Souza-Neves, Federico Cuadro, Richard Nunez-Olivera, Martina Crispo, Fabio R. Vasconcelos, Maria Julia B. Bezerra, Renato F. Silva, Laritza F. Lima, Jose Ricardo Figueiredo, Ivan C. Bustamante-Filho, Alejo Menchaca, Arlindo A. Moura
Summary: The present study evaluated the effects of in vitro maturation (IVM) on the proteome of cumulus-oocyte complexes (COCs) from ewes. The results showed that IVM caused upregulation and downregulation of proteins in COCs. Upregulated proteins in mature COCs after IVM were related to immune response, complement cascade, cell cycle, and extracellular matrix organization, while downregulated proteins in immature COCs after IVM were linked to metabolic processes, immune response, and complement cascade.
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2022)
Article
Multidisciplinary Sciences
Kendall A. Hyde, Francisco L. N. Aguiar, Paula B. Alvarenga, Amanda L. Rezende, Benner G. Alves, Kele A. Alves, Gustavo D. A. Gastal, Melba O. Gastal, Eduardo L. Gastal
Summary: This study investigated the clustering and neighborhood patterns of preantral follicles in the horse ovary, showing that younger mares have more clustering and neighbors compared to older mares. The clustering was found in all areas of the ovary, and follicles with neighbors were more likely to be morphologically normal.
Article
Reproductive Biology
A. F. B. Silva, L. F. Lima, A. N. P. Morais, L. L. Lienou, Y. F. Watanabe, D. C. Joaquim, S. M. Morais, D. R. Alves, A. F. Pereira, A. C. Santos, B. G. Alves, D. M. M. Padilha, E. L. Gastal, J. R. Figueiredo
Summary: This study investigates the effects of eugenol supplementation on bovine oocyte in vitro maturation and the subsequent in vitro embryo production and quality through IVF. The results show that adding 40μM eugenol to the maturation medium improves antioxidant capacity and cleavage rate. Additionally, lower calreticulin levels and higher total cell numbers in expanded blastocysts were observed in the lower eugenol concentration treatments. Based on these findings, enriching the maturation medium with 10μM eugenol is recommended for in vitro bovine embryo production.
Article
Agriculture, Dairy & Animal Science
Kamila S. Otavio, Jose R. S. Passos, Renato F. Silva, Laritza F. Lima, Jesus Cadenas, Victor M. Paes, Hudson H. Correia, Anna Clara A. Ferreira, Francisca G. Canafistula, Maria Julia B. Bezerra, Lais L. B. Oliveira, Guilherme G. C. Carvalho, Carlos R. K. Paier, Claudia Pessoa, Denise G. Guerreiro, Maria Elisabete A. Moraes, Jose Ricardo Figueiredo, Arlindo A. Moura
Summary: The study evaluated the proteome of early antral follicles from Ovis aries using LC-MS/MS analysis. A total of 2503 follicle proteins were identified, including abundant proteins such as vimentin, actin, lamin, heat shock proteins, and histones. miRNAs were found to modulate genes involved in cell cycle, cell differentiation, aging, apoptosis, adipocyte differentiation, and cell division in sheep follicles. This comprehensive atlas of proteins expressed in sheep early antral follicles will contribute to the identification of biomarkers for follicular development and oocyte maturation.
ANIMAL REPRODUCTION SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
L. V. S. Naupas, D. C. C. Brito, S. S. Souza, F. A. S. Brandao, R. F. Silva, C. C. L. Fernandes, G. M. Tetaping, B. G. Alves, J. Y. G. Silva, M. I. F. Guedes, D. Rondina, J. R. Figueiredo, A. P. R. Rodrigues
Summary: This study found that adding 100μM or 150μM ALA to the vitrification solution can protect sheep ovarian tissue, maintain follicle morphology, and promote follicular development after in vitro culture or autotransplantation. However, high levels of ALA may cause oxidative stress and tissue fibrosis after transplantation.
Article
Agriculture, Dairy & Animal Science
Luisa Miglio, Marco Aurelio Schiavo Novaes, Laritza Ferreira de Lima, Tatiane Moraes Arantes, Kele Amaral Alves, Francisca Elda Ferreira Dias, Marcelo Emilio Beletti, Jose Ricardo de Figueiredo
Summary: This study investigated the effects of zinc oxide nanoparticles functionalized with curcumin (ZnO(np) + CUR) on bovine in vitro embryo production and cellular antioxidant response during in vitro embryo culture (IVC). Cumulus-oocyte complexes (COCs) were matured, fertilized, and cultured in the presence of different concentrations of ZnO(np) + CUR. The results showed that the addition of 12μM ZnO(np) + CUR during IVC reduced intracellular ROS production and blastocyst production rate.
REPRODUCTION IN DOMESTIC ANIMALS
(2023)
Article
Cell Biology
A. N. P. Morais, L. F. Lima, A. F. B. Silva, L. L. Lienou, A. C. A. Ferreira, Y. F. Watanabe, D. C. Joaquim, B. G. Alves, A. F. Pereira, D. R. Alves, A. C. Oliveira, S. M. Morais, D. M. Magalhaes-Padilha, J. R. Figueiredo, E. L. Gastal
Summary: This study investigates the effects of carvacrol supplementation on in vitro maturation and embryo production in bovine oocytes. It was found that carvacrol supplementation increased the antioxidant capacity of the culture medium, but decreased the number of cells per blastocyst.
