Article
Reproductive Biology
Yao Xiao, Kyungjun Uh, Veronica M. Negron-Perez, Hannah Haines, Kiho Lee, Peter J. Hansen
Summary: Disruption of CSF2RA blocked most CSF2-induced changes in gene expression in the blastocyst, indicating that, despite the absence of the beta subunit of the receptor, the preimplantation embryo uses CSF2RA for signal transduction.
BIOLOGY OF REPRODUCTION
(2021)
Article
Agriculture, Dairy & Animal Science
Juan P. Cabeza, Juan Camera, Olinda Briski, Minerva Yauri Felipe, Daniel F. Salamone, Andres Gambini
Summary: This study investigates the use of a novel zinc chelator for activating bovine and porcine oocytes. The results show that the zinc chelator can successfully induce blastocyst development in both species, although developmental rates and embryo quality are compromised in bovine compared to calcium-induced embryos. On the contrary, the new concentration and incubation time of the zinc chelator improve developmental rates in porcine without affecting embryo quality. These findings contribute to understanding the role of zinc in oocyte activation and preimplantation embryo development across different mammalian species.
Article
Reproductive Biology
Angelica C. dos Santos, Daniel C. Joaquim, Ricardo P. Nociti, Carolina H. Macabelli, Rafael V. Sampaio, Aline S. Oliveira, Maico O. Pita, Robinson A. M. de Oliveira, Juliano C. da Silveira, Flavio V. Meirelles, Osnir Y. Watanabe, Yeda F. Watanabe, Marcos R. Chiaratti
Summary: Micro-vibration treatment during in vitro embryo culture improves the cryoresistance of bovine embryos, but it may also lead to epigenetic and transcriptional abnormalities.
Article
Cell Biology
Charles Banliat, Coline Mahe, Regis Lavigne, Emmanuelle Com, Charles Pineau, Valerie Labas, Benoit Guyonnet, Pascal Mermillod, Marie Saint-Dizier
Summary: A comprehensive study of proteome dynamics in non-rodent mammalian embryos developed in vivo was conducted. Proteomic changes in bovine embryos at different developmental stages were analyzed, and distinct patterns were observed. Proteins that increased in abundance during development were associated with RNA metabolism, protein translation, and ribosome biogenesis, while proteins that decreased in abundance were involved in Golgi vesicle transport and protein processing in endoplasmic reticulum. The results provide valuable insights into the molecular mechanisms underlying early embryo development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ahmed Atef Mesalam, Marwa El-Sheikh, Myeong-Don Joo, Atif Ali Khan Khalil, Ayman Mesalam, Mi-Jeong Ahn, Il-Keun Kong
Summary: Exposing bovine oocytes to juglone at concentrations of 12.5-50.0 μM significantly impairs their development by inducing ROS accumulation, apoptosis, and mitochondrial dysfunction. This study provides insights into the adverse effects of juglone treatment on oocyte maturation and embryo development, highlighting the importance of understanding the impact of traditional medicinal components on reproductive processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Klaudia Mietkiewska, Pawel Kordowitzki, Chandra S. Pareek
Summary: Heat stress poses a significant threat to cattle reproduction, impacting hormonal balance, oocyte quality, mitochondrial function, DNA integrity, and maternal transcription. It can also lead to blastocyst development disorders. Cows exposed to heat stress in utero experience lower milk yield, immunological disorders, lower birth weight, and shortened lifespan associated with accelerated aging. Understanding the effects of heat stress at the cellular and molecular levels is crucial for protecting cow reproduction.
Article
Reproductive Biology
I. Yousefian, A. Zare-Shahneh, A. Goodarzi, H. Baghshahi, A. A. Fouladi-Nashta
Summary: Adding MitoTEMPO significantly increased the proportion of oocytes at the MII stage and embryos at the blastocyst stage while decreasing intracellular GSH content and ROS levels in oocytes. Furthermore, the expression level of the pro-apoptotic gene BAX was reduced in blastocysts from the M1 group compared to the control group.
Review
Biochemistry & Molecular Biology
Fabiana de Andrade Melo-Sterza, Ralf Poehland
Summary: Lipids play a crucial role in the initial development of embryos, but excessive lipid droplet formation may be harmful to embryo cryotolerance. Additionally, increased lipid accumulation in in vitro produced embryos may be a response to stress, and there are still many questions regarding embryo lipid metabolism and developmental potential.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Shun Saito, Shota Yamamura, Nanami Kohri, Hanako Bai, Masashi Takahashi, Manabu Kawahara
Summary: WW domain-containing transcription regulator 1 (WWTR1) is a key player in the Hippo pathway and plays a crucial role in the differentiation of trophectoderm (TE) cells during preimplantation development. Knockdown of WWTR1 resulted in reduced TE cell numbers and expression of TE-specific genes in bovine embryos, highlighting the importance of WWTR1 in TE cell development.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Agriculture, Dairy & Animal Science
Lee D. Spate, Jie Yin, Lauren Sammel, Randall S. Prather, Bethany K. Redel
Summary: COVID-19 has had a significant impact on abattoirs worldwide, resulting in a critical loss of pig material for research. Commercial sources of pig oocytes are expensive and already operating at maximum capacity. This study presents an alternative source of oocytes that can produce live, healthy piglets.
REPRODUCTION IN DOMESTIC ANIMALS
(2023)
Article
Endocrinology & Metabolism
Desmond A. R. Tutt, Gizem Guven-Ates, Wing Yee Kwong, Rob Simmons, Fei Sang, Giuseppe Silvestri, Carla Canedo-Ribeiro, Alan H. Handyside, Remi Labrecque, Marc-Andre Sirard, Richard D. Emes, Darren K. Griffin, Kevin D. Sinclair
Summary: Removing complex proteins during bovine IVM affects the metabolism of cumulus cells. The addition of melatonin is beneficial in the presence of complex proteins but detrimental in their absence. These findings lay the foundation for developing chemically defined IVM systems for the generation of cytogenetically and epigenetically normal embryos.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Cell Biology
Nico G. Menjivar, Ahmed Gad, Samuel Gebremedhn, Soham Ghosh, Dawit Tesfaye
Summary: Climate change-induced global warming leads to hyperthermia in dairy and beef animals, impacting reproductive function. Extracellular vesicles (EVs) play a crucial role in regulating processes like folliculogenesis and signaling pathways. In this study, granulosa cell-derived EVs (GC-EVs) were tested for their ability to improve bovine oocytes' thermotolerance to heat stress (HS) during in vitro maturation (IVM). Results show that thermally stressed GCs release EVs (S-EVs) that protect oocytes from HS by reducing ROS accumulation, improving mitochondrial function, and suppressing stress-associated gene expression. Additionally, GC-EVs have a carryover effect on oocyte maturation and development to the blastocyst stage, enhancing viability.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Reproductive Biology
Thiago F. Amaral, Angela Gonella Diaza, Daniella Heredia, Gabriela D. Melo, Eliab Estrada-Cortes, Laura M. Jensen, Ky Pohler, Peter J. Hansen
Summary: DKK1 can alter the early developmental program of bovine embryos, affecting the number of trophectoderm cells and placental function. Treatment with DKK1 at the morula-to-blastocyst stages has long-term consequences on postnatal phenotype, resulting in heavier calves at birth but slower growth after birth.
BIOLOGY OF REPRODUCTION
(2022)
Article
Nutrition & Dietetics
Marwa El-Sheikh, Ayman Mesalam, Myeong-Don Joo, Tabinda Sidrat, Ahmed Atef Mesalam, Il-Keun Kong
Summary: This study investigates the biological effects of vanillic acid on early embryo development. The results demonstrate that the supplementation of vanillic acid improves blastocyst development, reduces oxidative stress, promotes fatty acid oxidation, and enhances mitochondrial activity. It also suggests a potential link between vanillic acid and the AKT signaling pathway, indicating its potential application in assisted reproductive technologies to improve human fertility.
Article
Biotechnology & Applied Microbiology
Charles Banliat, Coline Mahe, Regis Lavigne, Emmanuelle Com, Charles Pineau, Valerie Labas, Benoit Guyonnet, Pascal Mermillod, Marie Saint-Dizier
Summary: The study revealed significant differences in protein expression between in vivo and in vitro developed bovine embryos, with embryos developed in vivo internalizing large amounts of oviductin and other proteins early in development, potentially contributing to their higher developmental ability.
Article
Reproductive Biology
Kun Zhang, Sandeep K. Rajput, Kyung-Bon Lee, Dongliang Wang, Juncheng Huang, Joseph K. Folger, Jason G. Knott, Jiuzhen Zhang, George W. Smith
BIOLOGY OF REPRODUCTION
(2015)
Article
Reproductive Biology
Kun Zhang, Sandeep K. Rajput, Shaohua Wang, Joseph K. Folger, Jason G. Knott, George W. Smith
BIOLOGY OF REPRODUCTION
(2016)
Article
Biochemistry & Molecular Biology
Sunny Dholpuria, Manish Kumar, Sandeep Kumar, Parul Sarwalia, Sandeep Rajput, Rakesh Kumar, Sachinandan De, Tirtha K. Datta
JOURNAL OF CELLULAR BIOCHEMISTRY
(2017)
Article
Developmental Biology
Jacqelyn M. Hand, Kun Zhang, Lei Wang, Prasanthi P. Koganti, Kristen Mastrantoni, Sandeep K. Rajput, Mohamed Ashry, George W. Smith, Jianbo Yao
MECHANISMS OF DEVELOPMENT
(2017)
Article
Biochemistry & Molecular Biology
Kun Zhang, Han Wang, Sandeep K. Rajput, Joseph K. Folger, George W. Smith
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2018)
Article
Reproductive Biology
Mohamed Ashry, KyungBon Lee, Joseph K. Folger, Sandeep K. Rajput, George W. Smith
REPRODUCTIVE BIOLOGY
(2018)
Article
Endocrinology & Metabolism
Mohamed Ashry, Sandeep K. Rajput, Joseph K. Folger, Jason G. Knott, Nabil A. Hemeida, Omaima M. Kandil, Refaat S. Ragab, George W. Smith
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2018)
Article
Multidisciplinary Sciences
Bradford W. Daigneault, Sandeep Rajput, George W. Smith, Pablo J. Ross
SCIENTIFIC REPORTS
(2018)
Article
Multidisciplinary Sciences
Guo Zhenhua, Sandeep K. Rajput, Joseph K. Folger, Liu Di, Jason G. Knott, George W. Smith
Article
Biochemical Research Methods
Bradford W. Daigneault, Marcela Vilarino, Sandeep K. Rajput, Tristan Frum, George W. Smith, Pablo J. Ross
Article
Toxicology
Cagri Camsari, Joseph K. Folger, Sandeep K. Rajput, Devin McGee, Keith E. Latham, George W. Smith
TOXICOLOGICAL SCIENCES
(2019)
Article
Multidisciplinary Sciences
Rachel C. West, Hao Ming, Deirdre M. Logsdon, Jiangwen Sun, Sandeep K. Rajput, Rebecca A. Kile, William B. Schoolcraft, R. Michael Roberts, Rebecca L. Krisher, Zongliang Jiang, Ye Yuan
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Reproductive Biology
Sandeep K. Rajput, Chunyan Yang, Mohamed Ashry, Joseph K. Folger, Jason G. Knott, George W. Smith
BIOLOGY OF REPRODUCTION
(2020)
Article
Biochemical Research Methods
Bradford W. Daigneault, Sandeep K. Rajput, George W. Smith
Article
Multidisciplinary Sciences
Alison F. Ermisch, Jason R. Herrick, Rolando Pasquariello, McKenna C. Dyer, Sarah M. Lyons, Corey D. Broeckling, Sandeep K. Rajput, William B. Schoolcraft, Rebecca L. Krisher
SCIENTIFIC REPORTS
(2020)