Article
Agriculture, Dairy & Animal Science
Malavika K. Adur, Jacob T. Seibert, Matthew R. Romoser, Katie L. Bidne, Lance H. Baumgard, Aileen F. Keating, Jason W. Ross
Summary: This study evaluated the impact of heat stress on the abundance of heat shock proteins (HSP) in the porcine endometrium, and investigated the regulatory effects of treatment methods on HSP. The results demonstrated significant changes in the abundance of HSP in the porcine endometrium in response to heat stress and progestin supplementation.
JOURNAL OF ANIMAL SCIENCE
(2022)
Article
Multidisciplinary Sciences
Ewa Monika Drzewiecka, Wiktoria Kozlowska, Agata Zmijewska, Anita Franczak
Summary: This study revealed that dietary restriction during the peri-conceptional period causes changes in gene expression in the pig myometrium, particularly those involved in endocrine activity, embryo-maternal interactions, and the regulation of cell cycle and proliferation.
SCIENTIFIC REPORTS
(2021)
Article
Agriculture, Dairy & Animal Science
Sarah R. McCoski, Rebecca R. Cockrum, Alan D. Ealy
Summary: Exposure to maternal obesity during pregnancy can have significant effects on offspring development, which may not be apparent until adulthood. This study investigated how obesity influences gene expression in the endometrium during early pregnancy using a sheep model. The results showed that obesity affects gene expression related to the immune response, adhesion, and angiogenesis in the endometrium. These findings suggest that the peri-implantation endometrium is responsive to maternal obesity and changes in gene expression during early embryogenesis may contribute to developmental programming.
JOURNAL OF ANIMAL SCIENCE
(2022)
Article
Genetics & Heredity
Renwu Hua, Qiaorui Liu, Weisi Lian, Dengying Gao, Cheng Huang, Minggang Lei
Summary: This study reveals the regulatory mechanism of extracellular vesicles (EVs) in uterine fluids on endometrial epithelial cells (EECs) and embryonic trophoblast cells (PTr2 cells). Transcriptome sequencing analysis identifies a significant number of differentially expressed genes in both EECs and PTr2 cells after treatment. Real-time quantitative PCR (RT-qPCR) validation confirms the differential expression of these genes. Additionally, bioinformatics analysis reveals the functional involvement of these differentially expressed genes.
Article
Multidisciplinary Sciences
Diana Monsivais, Takashi Nagashima, Renata Prunskaite-Hyyrylainen, Kaori Nozawa, Keisuke Shimada, Suni Tang, Clark Hamor, Julio E. Agno, Fengju Chen, Ramya P. Masand, Steven L. Young, Chad J. Creighton, Francesco J. DeMayo, Masahito Ikawa, Se-Jin Lee, Martin M. Matzuk
Summary: The study reveals that bone morphogenetic proteins control endometrial receptivity in mice through the ACVR2A and SMAD1/5 signaling pathway, with ACVR2B being dispensable during embryo implantation.
NATURE COMMUNICATIONS
(2021)
Article
Reproductive Biology
Qun Hu, Xupeng Zang, Yue Ding, Ting Gu, Junsong Shi, Zicong Li, Gengyuan Cai, Dewu Liu, Zhenfang Wu, Linjun Hong
Summary: This study investigated the characteristics, origin, and roles of uterine luminal fluid-derived extracellular vesicles (EVs) in porcine conceptus implantation. The EVs were found to mainly originate from endometrial epithelial cells, with an increase in concentration starting from day 12 of pregnancy. Functional enrichment analysis revealed that the miRNAs in these EVs potentially regulate EV transportation or conceptus implantation, providing important insights for future translational studies on porcine EVs.
Article
Pharmacology & Pharmacy
Yong -Jiang Zhou, Qian-Feng Qiao, Li-Qing Wang, Tao -Yu Sheng, Man-Xue Cui, Qi-Duo Chen, Can-Yang Wang, Yun-Xiao Zhang
Summary: Beta-cypermethrin, a widely used pyrethroid pesticide, can impair endometrial remodeling in mice, but the mechanism is not well understood. In this study, researchers investigated the mechanism by which peri-implantation beta-cypermethrin exposure reduces uterine remodeling in pregnant mice. They found that beta-cypermethrin decreased the expression of endometrial remodeling markers and inhibited key molecules in the PI3K/Akt/mTOR signaling pathway. The results suggest that reducing the PI3K/Akt/mTOR pathway may enhance defective endometrial remodeling induced by beta-cypermethrin exposure.
Article
Endocrinology & Metabolism
Runan Hu, Yanjing Huang, Yufan Song, Xiao Wu, Kunkun Song, Guangying Huang, Mingmin Zhang, Haoxu Dong
Summary: This study aimed to investigate the effect of acupuncture on improving the pregnancy rate of COH rats by regulating the opening time of the implantation window and endometrial receptivity. Acupuncture was performed on COH rats at specific acupoints for 7 times. The results showed that acupuncture significantly increased the pregnancy rate, restored abnormal hormone levels, and reversed the advancement of implantation window in COH rats. Additionally, acupuncture restored the expression levels of ER, PR, LIF, integrin beta 3, VEGF, and FGF-2 in the endometrium to varying degrees. Therefore, acupuncture may enhance endometrial receptivity and improve the pregnancy rate of COH rats.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Genetics & Heredity
Renwu Hua, Yueying Wang, Weisi Lian, Wenchao Li, Yu Xi, Songyi Xue, Tingting Kang, Minggang Lei
Summary: This study analyzed the small RNA expression profiles of EVs from porcine uterine flushing fluids during peri-implantation. Differential expression of miRNAs was found to be involved in key processes related to immunization, endometrial receptivity, and embryo development, which are important for embryonic implantation. The research revealed the potential significant effects of small RNAs in EVs on implantation in pigs and provided resources for further studies on miRNAs and piRNAs in the communication between embryo and endometrium.
Article
Immunology
Ingrid Granne, Mengni Shen, Helena Rodriguez-Caro, Gurmeher Chadha, Elizabeth O'Donnell, Jan J. Brosens, Siobhan Quenby, Tim Child, Jennifer H. Southcombe
Summary: Recurrent Pregnancy Loss (RPL) affects 2-4% of couples, with risk of miscarrying a euploid pregnancy increased by lethal embryonic aneuploidy. Conditions associated with RPL include immune pathologies, with T regulatory cells as strong candidates for dysregulation. Maternal acquired immune memory of pregnancy within the Treg population is indicated by altered endometrial Treg transcriptome in parous women compared to nulliparous.
MUCOSAL IMMUNOLOGY
(2022)
Article
Reproductive Biology
Wiktoria Kozlowska, Ewa Monika Drzewiecka, Agata Zmijewska, Anita Franczak
Summary: The study aims to investigate the effects of EMF exposure on estrogen synthesis and release in the endometrium. The results suggest that EMF exposure affects gene expression and is related to P-4 treatment. Additionally, EMF disrupts the synthesis and release of E-1 and E-2 in the endometrium.
REPRODUCTIVE BIOLOGY
(2022)
Article
Pediatrics
Shanshan Liu, Lining Guo, Min Chen, Wei Liu, Yanhong Li, Xiaoxu Wang, Shilan Li, Jie Zhang, Xin Ni
Summary: This study found the presence of caspase-1, IL-1 beta, and IL-18 in the middle ear effusion of children with OME, but these levels did not significantly differ between mucoid and serous samples or between those with and without a history of ventilation tube insertion. IL-1 beta and IL-18 levels showed a significant negative correlation with the duration of OME, indicating a potential role of these cytokines in the inflammatory process.
FRONTIERS IN PEDIATRICS
(2021)
Article
Reproductive Biology
Sudikshya Paudel, Bangmin Liu, Magdalina J. Cummings, Kelsey E. Quinn, Fuller W. Bazer, Kathleen M. Caron, Xiaoqiu Wang
Summary: The study revealed higher levels of ADM in uterine fluid of pregnant pigs, indicating its significant role in implantation and conceptus growth. Additionally, the increased expression of CALCRL, RAMP2, and ACKR3 in pregnant pig conceptuses suggests the important influence of ADM on uterine receptivity and embryo development during pregnancy.
BIOLOGY OF REPRODUCTION
(2021)
Article
Physiology
M. Martyniak, E. M. Drzewiecka, A. Zmijewska, A. Franczak, G. Kotwica
Summary: This study found that the gene expression in the epithelial cells of the ampulla and isthmus of the oviducts in pigs was altered during the peri-conceptional period, possibly due to DNA methylation. By evaluating important genes related to embryo development and determining the level of DNA methylation, it was found that the methylation levels of certain genes have changed. This suggests that the presence of embryos in the oviduct can change the transcriptomic profile and DNA methylation level in the epithelial cells.
JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Liang-Hsuan Chen, She-Hung Chan, Chin-Jung Li, Hsien-Ming Wu, Hong-Yuan Huang
Summary: Adenomyosis patients show significant alterations in the IL-18 system, with down-regulation of IL-18BP potentially contributing to local immune dysregulation. The abnormal expression of the IL-18 system may impact cytokine networks and play a role in the pathogenesis of adenomyosis.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2022)
Article
Agriculture, Multidisciplinary
Yiling Ke, Rachel M. Mitacek, Anupam Abraham, Gretchen G. Mafi, Deborah L. VanOverbeke, Udaya DeSilva, Ranjith Ramanathan
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2017)
Article
Agriculture, Dairy & Animal Science
Cashley M. Ahlberg, Kristi Allwardt, Ashley Broocks, Kelsey Bruno, Levi McPhillips, Alexandra Taylor, Clint R. Krehbiel, Michelle Calvo-Lorenzo, Chris J. Richards, Sara E. Place, Udaya DeSilva, Deborah L. VanOverbeke, Raluca G. Mateescu, Larry A. Kuehn, Robert L. Weaber, Jennifer M. Bormann, Megan M. Rolf
JOURNAL OF ANIMAL SCIENCE
(2018)
Article
Microbiology
Daihua Qi, Xuwen Wieneke, Jianping Tao, Xu Zhou, Udaya Desilva
FRONTIERS IN MICROBIOLOGY
(2018)
Article
Agriculture, Dairy & Animal Science
Cashley M. Ahlberg, Kristi Allwardt, Ashley Broocks, Kelsey Bruno, Levi McPhillips, Alexandra Taylor, Clint R. Krehbiel, Michelle S. Calvo-Lorenzo, Chris J. Richards, Sara E. Place Paragraph, Udaya DeSilva, Deborah L. VanOverbeke, Raluca G. Mateescu, Larry A. Kuehn, Robert L. Weaber, Jennifer M. Bormann, Megan M. Rolf
JOURNAL OF ANIMAL SCIENCE
(2018)
Article
Multidisciplinary Sciences
C. C. Lyman, G. R. Holyoak, K. Meinkoth, X. Wieneke, K. A. Chillemi, U. DeSilva
Article
Agriculture, Dairy & Animal Science
Cashley M. Ahlberg, Kristi Allwardt, Ashley Broocks, Kelsey Bruno, Alexandra Taylor, Levi McPhillips, Clint R. Krehbiel, Michelle Calvo-Lorenzo, Chris J. Richards, Sara E. Place, Udaya DeSilva, Deborah L. VanOverbeke, Raluca G. Mateescu, Larry A. Kuehn, Robert Weaber, Jennifer Bormann, Megan M. Rolf
JOURNAL OF ANIMAL SCIENCE
(2019)
Article
Multidisciplinary Sciences
Shelby Spring, Hasitha Premathilake, Udaya DeSilva, Cedrick Shili, Scott Carter, Adel Pezeshki
SCIENTIFIC REPORTS
(2020)
Article
Agriculture, Multidisciplinary
Ranjith Ramanathan, Frank Kiyimba, John Gonzalez, Gretchen Mafi, Udaya DeSilva
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2020)
Article
Multidisciplinary Sciences
Shelby Spring, Hasitha Premathilake, Chloe Bradway, Cedrick Shili, Udaya DeSilva, Scott Carter, Adel Pezeshki
SCIENTIFIC REPORTS
(2020)
Article
Multidisciplinary Sciences
Daihua Qi, Xuwen Wieneke, Peipei Xue, Li He, Udaya DeSilva
Summary: This study reveals changes in soil fungal communities in response to KRD progression in southwest China, with significant differences found between Ascomycota and Basidiomycota. The ratio of Ascomycota/Basidiomycota may be used as an indicator of KRD severity, while the genus Sebacina shows potential for promoting vegetation and land restoration in KRD areas.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
G. R. Holyoak, H. U. Premathilake, C. C. Lyman, J. L. Sones, A. Gunn, X. Wieneke, U. DeSilva
Summary: The goal of this study was to understand the composition and existence of the resident uterine microbiome in healthy mares and to establish the presence of a core microbiome for the healthy equine uterus. Results showed that the composition of the equine uterine microbiome is largely dependent on the geographical location of the animal, with a core microbiome consisting of several genera.
SCIENTIFIC REPORTS
(2022)
Article
Agriculture, Dairy & Animal Science
Andrew D. Lakamp, Cashley M. Ahlberg, Kristi Allwardt, Ashely Broocks, Kelsey Bruno, Levi Mcphillips, Alexandra Taylor, Clint R. Krehbiel, Michelle S. Calvo-Lorenzo, Chris Richards, Sara E. Place, Udaya Desilva, Larry A. Kuehn, Robert L. Weaber, Jennifer M. Bormann, Megan M. Rolf
Summary: Predicted methane traits are heritable and easy to calculate, making them potential candidates for selecting animals with low methane production. Using a mathematical model to predict methane production can overcome the difficulties and expenses of direct measurement, and animals can be ranked similarly regardless of the model used. A genome-wide association study identified significant loci and genes related to predicted methane production, including oxidoreductase activity and collagen production.
JOURNAL OF ANIMAL SCIENCE
(2023)
Article
Food Science & Technology
Caitlin Karolenko, Udaya DeSilva, Peter M. Muriana
Summary: Biltong is a South African air-dried beef product that relies on marinade chemistry and drying to reduce microbial growth. The microbial community changes during biltong processing, with a reduction in diversity. The main bacteria genera identified were Latilactobacillus sp., Lactococcus sp., and Carnobacterium sp. The psychrotrophic bloom during refrigerated storage of raw beef may suppress the growth of mesophilic foodborne pathogens and contribute to the safety of biltong.
Article
Agriculture, Dairy & Animal Science
Elizabeth A. Dressler, William Shaffer, Kelsey Bruno, Clint R. Krehbiel, Michelle Calvo-Lorenzo, Chris J. Richards, Sara E. Place, Udaya DeSilva, Larry A. Kuehn, Robert L. Weaber, Jennifer M. Bormann, Megan M. Rolf
Summary: This study evaluated novel phenotypes related to feed and water intake behaviors and estimated their heritabilities, variance components, and genetic and phenotypic correlations. Understanding the importance of feed and water intake is crucial for cattle production as it impacts producer profitability and sustainability. While previous studies have focused on feed and water intake, the associated feeding and drinking behaviors are relatively unknown and lack standardized phenotypes. This study assessed five behaviors of feed and water intake using individual animal records and identified significant single nucleotide polymorphisms for these behaviors.
JOURNAL OF ANIMAL SCIENCE
(2023)
Article
Veterinary Sciences
Eryn B. Hanak, Nicola Di Girolamo, Udaya DeSilva, Rachel E. Marschang, Joao L. Brandao, Isabelle L. L. Desprez, Jennifer Campbell, Andrew E. Moore, Jody P. Lulich
Summary: The study compared the mineral types of naturally occurring uroliths in ferrets from North America, Europe, and Asia, and found a significant increase in the proportion of cystine uroliths in North America between 2010 and 2018. Urgent efforts are needed to determine the underlying causes and mitigate cystine urolithiasis in ferrets.
JAVMA-JOURNAL OF THE AMERICAN VETERINARY MEDICAL ASSOCIATION
(2021)