Article
Agriculture, Dairy & Animal Science
Sang-Hee Lee, Seunghyung Lee
Summary: This study aimed to investigate the effects of melatonin and silymarin on the motility and viability of frozen-thawed boar semen, as well as the production of reactive oxygen species (ROS) and nitric oxide (NO). The results showed that melatonin and silymarin reduced ROS and NO production in frozen-thawed sperm and enhanced sperm viability. Therefore, melatonin and silymarin play an important role as antioxidants in semen cryopreservation.
Article
Veterinary Sciences
Mohammad Reza Divar, Mehdi Azari, Asghar Mogheiseh, Sadegh Ghahramani
Summary: Melatonin has a protective effect on canine sperm during freezing and cooling, improving sperm motility, viability, and DNA integrity.
BMC VETERINARY RESEARCH
(2022)
Article
Environmental Sciences
Zi Teng, YaNan Liu, ZeLin Wang, YuSheng Zhang, MeiNa Xie, Peng Zhu, Ning Li, FuJun Liu, XueXia Liu
Summary: Bisphenol A (BPA) exposure negatively affects male fertility, while melatonin (MLT) treatment can alleviate these effects by regulating antioxidant molecules, inhibiting germ cell apoptosis, and maintaining male fertility. This study provides more insights into the molecular mechanisms of BPA and MLT in male reproduction.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Endocrinology & Metabolism
Teng Zi, YaNan Liu, YuSheng Zhang, ZeLin Wang, ZhiXin Wang, Song Zhan, Zhu Peng, Ning Li, XueXia Liu, FuJun Liu
Summary: This study found that doxorubicin (DOX) has adverse effects on spermatogenesis and sperm function, but melatonin (MLT) can partially protect the quality and quantity of sperm and reduce the damage caused by DOX. MLT enhances sperm development and reduces apoptosis by reversing the effects of DOX on the testes, thereby protecting against DOX-induced reproductive toxicity.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Melissa Carvajal-Serna, Jaime Antonio Cardozo-Cerquera, Henry Alberto Grajales-Lombana, Adriana Casao, Rosaura Perez-Pe
Summary: This study demonstrates variations in melatonin concentration in seminal plasma between rainy and dry seasons in three Colombian sheep breeds, and investigates how melatonin modulates sperm capacitation in equatorial-located breeds. The addition of melatonin to the cocktail medium is shown to partially prevent the increase in capacitated spermatozoa across breeds and seasons, shedding light on the direct effects of melatonin on spermatozoa in ovine breeds under equatorial photoperiod conditions.
Article
Reproductive Biology
Priscila C. Palhares, Isadora de L. Assis, Gilmara J. Machado, Renata M. P. de Freitas, Mariella B. D. de Freitas, Daniella A. J. Paula, William F. Carneiro, Naiara C. Motta, Luis D. S. Murgas
Summary: This study aimed to evaluate the effects of melatonin supplementation and freezing curves on cryopreserved Brycon orbignyanus milt. Results showed that solutions with melatonin had better values in vitality, morphology, motility, and fertilization rate, with the 2 mM melatonin and slow curve yielding the best outcomes.
Article
Reproductive Biology
Minghui Yang, Shengyu Guan, Jingli Tao, Kuanfeng Zhu, Dongying Lv, Jing Wang, Guangdong Li, Yuefeng Gao, Hao Wu, Jinghao Liu, Lin Cao, Yao Fu, Pengyun Ji, Zhengxing Lian, Lu Zhang, Guoshi Liu
Summary: The study found that endogenous melatonin can regulate male reproductive function by inhibiting Leydig cell apoptosis, increasing testosterone production, and improving sperm quality. Melatonin exerts these effects by targeting the mitochondrial apoptotic pathway and upregulating testosterone synthesis-related genes in Leydig cells.
BIOLOGY OF REPRODUCTION
(2021)
Article
Biochemistry & Molecular Biology
Julian Santiago-Moreno, Belen Pequeno, Belen Martinez-Madrid, Cristina Castano, Paula Boveda, Rosario Velazquez, Adolfo Toledano-Diaz, Manuel Alvarez-Rodriguez, Heriberto Rodriguez-Martinez
Summary: This study identified the presence of AQPs in frozen-thawed sperm of wild ruminants and investigated the influence of the interaction between photoperiod and thyroxine on AQP expression and testosterone secretion. The results showed that thyroxine and melatonin treatments can significantly alter sperm viability and the distribution of AQPs. Furthermore, the study suggested that the impact of photoperiod/melatonin on AQP expression might be indirectly exerted through changes in testosterone concentration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Veterinary Sciences
Ali Salama, Elshymaa A. Abdelnaby, Ibrahim A. Emam, Mohamed Fathi
Summary: This study aimed to investigate the effects of melatonin administration on testicular vascular perfusion, steroid hormones, and semen characteristics in dogs. The results demonstrated that a single melatonin administration could improve semen volume, concentration, sperm motility, and total sperm count, as well as enhance testicular vascularization and intratesticular colored areas. Furthermore, melatonin administration increased testosterone, estradiol, and nitric oxide levels.
BMC VETERINARY RESEARCH
(2022)
Article
Agriculture, Dairy & Animal Science
Estela Fernandez-Alegre, Estibaliz Lacalle, Cristina Soriano-Ubeda, Juan Carlos Dominguez, Adriana Casao, Felipe Martinez-Pastor
Summary: Melatonin affects sperm physiology through membrane receptors, mainly protecting sperm from apoptosis and maintaining acrosomal integrity. Intracellular calcium was not affected, differing from other studies. This study supports the relevance of melatonin on sperm physiology and could contribute to the application of reproductive technologies in wild ruminants.
REPRODUCTION IN DOMESTIC ANIMALS
(2022)
Article
Biochemistry & Molecular Biology
Joel R. Drevet, Jorge Hallak, Mohammad-Hossein Nasr-Esfahani, Robert J. Aitken
Summary: This review explores the relationship between spermatozoa and reactive oxygen species (ROS), highlighting how spermatozoa rely on ROS for maturity but are highly susceptible to oxidative damage. Recent studies have shown the significant impact of oxidative damage on sperm structures and functions, particularly in terms of fertilization and embryonic development. Future research aims to investigate whether pro-oxidative situations can lead to epigenetic changes in spermatozoa.
ANTIOXIDANTS & REDOX SIGNALING
(2022)
Article
Reproductive Biology
Abdallah M. Shahat, Jacob C. Thundathil, John P. Kastelic
Summary: Melatonin improves testicular blood flow and protects sperm motility and morphology in rams exposed to testicular heat stress.
Review
Biochemistry & Molecular Biology
Annie Langston-Cox, Sarah A. Marshall, Daisy Lu, Kirsten R. Palmer, Euan M. Wallace
Summary: Preeclampsia is a pregnancy-specific disease characterized by hypertension and organ dysfunction. Traditional treatment has focused on antihypertensive medications, but recent insights into the pathogenesis have introduced new options such as melatonin. Melatonin, as a safe antioxidant, shows promise in improving maternal condition and protecting the fetus.
Article
Food Science & Technology
Huifeng Luo, Rongxiu Liu, Yilin Lang, Jinhui Zhao, Cuicui Zhuang, Jundong Wang, Chen Liang, Jianhai Zhang
Summary: This study aims to explore the role of melatonin in mitigating fluoride-induced male reproductive toxicity through the IL-17A pathway, and identify potential targets. Wild type and IL-17A knockout mice were used, and the results showed that melatonin supplementation alleviated fluoride-induced impairment of spermatogenesis and maturation process, protecting the morphology of testis and epididymis. Tesk1 and Pten were identified as candidate targets from 29 regulation genes.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
K. R. Pool, J. P. Rickard, S. P. de Graaf
Summary: The study demonstrated that melatonin does not protect the quality of cryopreserved spermatozoa through indiscriminate scavenging of reactive oxygen species, but specifically reduces mitochondrial superoxide production, altering sperm functionality.
DOMESTIC ANIMAL ENDOCRINOLOGY
(2021)
Article
Biology
Jose A. Abecia, Marianne Gave, Ana I. Garcia, Adriana Casao, Melissa Carvaja-lSerna, Carlos Palacios, Matthieu Keller, Philippe Chemineau, Jose A. Delgadillo
Summary: The study showed that autumn-born ram-lambs reared in isolation from adult congeners exhibited higher sexual activity and fertility at 8 months of age, indicating that no special rearing strategy is necessary to induce puberty in these rams.
BIOLOGICAL RHYTHM RESEARCH
(2021)
Article
Agriculture, Dairy & Animal Science
S. Gimeno-Martos, M. Santorroman-Nuez, J. A. Cebrian-Perez, T. Muino-Blanco, R. Perez-Pe, A. Casao
Summary: The steroid hormones E2 and P4 can induce the acrosome reaction in ram spermatozoa through receptor-mediated mechanisms. Estrogen receptors (ER) and progesterone receptors (PR) are present in these cells, suggesting a receptor-mediated effect of the hormones on sperm function.
DOMESTIC ANIMAL ENDOCRINOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Juliana de Carvalho Delgado, Thais Rose dos Santos Hamilton, Camilla Mota Mendes, Adriano Felipe Perez Siqueira, Marcelo Demarchi Goissis, Jose Buratini, Mayra Elena Ortiz D'Avila Assumpcao
Summary: The study shows that BMP15 and FGF16 have significant effects on cumulus cell expansion, DNA fragmentation, oocyte nuclear maturation, energy metabolism, and progesterone production in bovine IVM, with BMP15 playing a particularly important role in the process.
REPRODUCTION IN DOMESTIC ANIMALS
(2021)
Article
Cell Biology
Sara Miguel-Jimenez, Blanca Pina-Beltran, Silvia Gimeno-Martos, Melissa Carvajal-Serna, Adriana Casao, Rosaura Perez-Pe
Summary: This study found NOX5 in ram spermatozoa and demonstrated that melatonin can prevent changes related to sperm capacitation by modulating NOX5 activity. This suggests that melatonin plays a role in antioxidant defense in ram sperm by influencing NOX5 during capacitation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Veterinary Sciences
Noelia Mendoza, Adriana Casao, Juan Domingo, Francisco Quintin, Adolfo Lavina, Enrique Fantova, Jose Alvaro Cebrian-Perez, Teresa Muino-Blanco, Rosaura Perez-Pe
Summary: This study explored the impact of non-traditional sperm quality markers in seminal doses on fertilizing ability, identifying intact membrane, non-capacitated spermatozoa, and DNA-intact spermatozoa as potential markers to discard males in AI programs. This is crucial for achieving satisfactory fertility rates from an economic point of view.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
Melissa Carvajal-Serna, Jaime Antonio Cardozo-Cerquera, Henry Alberto Grajales-Lombana, Adriana Casao, Rosaura Perez-Pe
Summary: This study demonstrates variations in melatonin concentration in seminal plasma between rainy and dry seasons in three Colombian sheep breeds, and investigates how melatonin modulates sperm capacitation in equatorial-located breeds. The addition of melatonin to the cocktail medium is shown to partially prevent the increase in capacitated spermatozoa across breeds and seasons, shedding light on the direct effects of melatonin on spermatozoa in ovine breeds under equatorial photoperiod conditions.
Article
Biology
Melissa Carvajal-Serna, Sara Miguel-Jimenez, Rosaura Perez-Pe, Adriana Casao
Summary: Testicular ultrasound can be a valuable tool for predicting ram seminal quality, with white and grey pixels correlating with poor sperm parameters and tubular density and lumen area correlating with good sperm quality. Ultrasound-video analysis allows for both macroscopic and microscopic evaluation of testicular echotexture, providing a non-invasive method for assessing ram fertility.
Article
Cell Biology
Felipe Eduardo Luedke, Caroline Pereira da Costa, Camilla Mota Mendes, Thais Rose dos Santos Hamilton, Marcella Pecora Milazzotto, Mayra E. O. A. Assumpcao, Marcelo Demarchi Goissis
Summary: This study found that supplementation of fetal bovine serum (FBS) in embryonic culture medium can affect cell differentiation during early bovine embryo development. Absence of FBS reduces blastocyst rates, but there is no reduction when 33% volume renewal of the medium is done at 90 hours post insemination. Additionally, FBS supplementation alters the allocation of trophectoderm (TE) and primitive endoderm cells.
Article
Developmental Biology
Sara Miguel-Jimenez, Melissa Carvajal-Serna, Victoria Pena-Delgado, Adriana Casao, Rosaura Perez-Pe
Summary: This study investigates the effect of melatonin and nitric oxide on capacitation and apoptotic-like changes in EGF-capacitated ram spermatozoa. The results indicate that NO levels and apoptotic-related markers increase after EGF incubation. Melatonin has a bimodal role in sperm EGF-capacitation, but neither of the two concentrations prevents the increase in apoptotic-like markers or NO levels. However, melatonin at 1μM prevents the activation of JNK.
REPRODUCTION FERTILITY AND DEVELOPMENT
(2022)
Article
Reproductive Biology
Sara Miguel-Jimenez, Sonia Borao, Virginia Portoles-Bayod, Adriana Casao, Rosaura Perez-Pe
Summary: The study investigated the effects of season and sperm capacitation state on the chemotactic response of ram spermatozoa to melatonin. The results showed that melatonin can enhance sperm recovery rate and capacitation, indicating a potential chemotactic effect of melatonin on ram spermatozoa.
Article
Agriculture, Dairy & Animal Science
Victoria Pena-Delgado, Melissa Carvajal-Serna, Manuel Fondevila, Maria A. Martin-Cabrejas, Yolanda Aguilera, Gerardo Alvarez-Rivera, Jose A. Abecia, Adriana Casao, Rosaura Perez-Pe
Summary: The aim of this study was to evaluate the effect of a phytomelatonin-rich diet on ram sperm quality and seminal plasma composition. The results showed that the diet, including grape pulp, pomegranate and tomato pomaces, increased melatonin levels in seminal plasma, improved sperm viability and morphology, and protected sperm cells against oxidative damage.
Article
Cell Biology
A. R. Moura, A. R. Santos, J. D. A. Losano, A. F. P. Siqueira, T. R. S. Hamilton, R. Zanella, K. C. Caires, R. Simoes
Summary: Our study found that Wagyu bulls have lower sperm motility compared to Nellore and Angus bulls, but did not differ in terms of plasma and acrosome membranes integrity, mitochondrial potential, chromatin resistance, sperm lipid peroxidation, or hormone (LH and testosterone) concentrations.
Editorial Material
Biology
Pilar Santolaria, Jessica P. Rickard, Rosaura Perez-Pe
Review
Agriculture, Dairy & Animal Science
Carlos Alonso Paco Nagaki, Thais Rose dos Santos Hamilton, Mayra Elena Ortiz D Avila Assumpcao
Summary: The evaluation of sperm chromatin plays a crucial role in predicting bull reproductive capacity, although the physiological mechanisms of protamination are still poorly understood.
ANIMAL REPRODUCTION
(2022)
