Article
Multidisciplinary Sciences
Natalija Topic Popovic, Slavko Kepec, Snjezana P. Kazazic, Ivancica Strunjak-Perovic, Krunoslav Bojanic, Rozelindra Coz-Rakovac
Summary: This study evaluated the use of the API20E biochemical identification system as a supportive tool for enhancing the discrimination of environmental bacteria by the MALDI-TOF MS method. The results showed that the API20E system can effectively identify bacterial genera, but there are issues with misidentification. In environmental isolates with lower identification scores, the API20E tests can be used as a purposeful and inexpensive identification support.
Article
Chemistry, Analytical
Xufang Hu, Zongping Wang, Haolin Chen, An Zhao, Nianrong Sun, Chunhui Deng
Summary: An advanced metabolic analysis tool based on highly porous metal oxides derived from metal-organic frameworks (MOFs) is developed in this study. The tool successfully differentiates and stages renal cell carcinoma (RCC), as well as identifies the size of tumors. This achievement shows promising prospects for designer matrix-based analytical tools in disease diagnosis.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Christoph Bookmeyer, Ulrich Roehling, Klaus Dreisewerd, Jens Soltwisch
Summary: Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is a rapidly growing method in the life sciences. The study introduces a novel post-ionization scheme based on single-photon induced chemical ionization using pulsed RF-Kr lamps, which effectively improves the ionization efficiency for analytes. Through the interplay with chemical dopants, the signal of various lipid molecules from mammalian tissue sections was significantly boosted.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Developmental Biology
Giulia Fiorentino, Andrew Smith, Giovanna Nicora, Riccardo Bellazzi, Fulvio Magni, Silvia Garagna, Maurizio Zuccotti
Summary: In this study, a spatial proteomics approach was used to analyze the protein composition of follicles at different growth stages in the mouse ovary. Proteins involved in the mutual relationship among oocytes, follicle cells, stroma, and the vascular network were identified and their quantitative changes during follicular differentiation were determined, providing important insights into folliculogenesis.
MOLECULAR HUMAN REPRODUCTION
(2023)
Article
Chemistry, Multidisciplinary
Ouyang Dan, Chuanzhe Wang, Chao Zhong, Juan Lin, Gang Xu, Guane Wang, Zian Lin
Summary: In this study, novel LDI-MS substrates were designed to enhance the sensitivity of mass signals and achieve high accuracy diagnosis of different saccharides and central precocious puberty (CPP).
Review
Chemistry, Applied
Mariangela Valletta, Sara Ragucci, Nicola Landi, Antimo Di Maro, Paolo Vincenzo Pedone, Rosita Russo, Angela Chambery
Summary: Mass spectrometry (MS) methodologies are increasingly used in food authentication and traceability due to their unparalleled specificity, accuracy, and sensitivity. Protein and peptide profiling using MS has become more consolidated and is being utilized to assess food traceability and authenticity. Recent applications of MS in protein/peptide analyses in food matrices show potential in ensuring the quality of agro-food products.
Review
Biochemistry & Molecular Biology
Yijia Wang, Amanda B. Hummon
Summary: MSI is a powerful imaging tool for drug discovery, capable of analyzing a wide range of molecules in diverse samples. Its potential has been demonstrated in 3D cell culture systems, providing insights into drug compound metabolism and distribution of metabolites. The application of MSI in patient-derived organoids for personalized medicine options is also discussed in this review.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Medicine, General & Internal
Yousun Chung, Minje Han, Jae-Seok Kim
Summary: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been widely used for microbial identification, with the study showing that the performance of ASTA MicroIDSys is comparable to that of the widely used Bruker Biotyper. The study evaluated the microbial identification results from both systems and found that ASTA MicroIDSys showed reliable performance in clinical microbiology laboratories.
Review
Microbiology
Xin-Fei Chen, Xin Hou, Meng Xiao, Li Zhang, Jing-Wei Cheng, Meng-Lan Zhou, Jing-Jing Huang, Jing-Jia Zhang, Ying-Chun Xu, Po-Ren Hsueh
Summary: MALDI-TOF MS has been used in clinical microbiology since 2010, offering advantages such as convenience, speed, accuracy, and low cost. It has shown promising results in rapid detection of highly virulent and drug-resistant pathogens, making it a valuable tool in clinical microbial identification.
Article
Chemistry, Applied
Zhaoliang Chai, Hongyan Bi
Summary: In this study, anti-E. coli functionalized magnetic beads were constructed and successfully used to capture E. coli from fish sarcoplasmic protein and fish muscle protein extracts. The reproducibility and accuracy of the method were demonstrated through experiments. The method showed high specific detection of target bacteria in complex biological samples.
Article
Chemistry, Physical
Dantong Zhao, Chunxia Ma, Meng Gao, Yong Li, Bo Yang, Hui Li, Runhao Zhang, Minglu Hao, Jing Huang, Kang Liang, Pu Chen, Lei Xie, Rong Rong, Biao Kong
Summary: This study developed sandwich-like gold nanoparticles@mesoporous silica nanocomposite@silver nanoparticles (Au@MSN@Ag) nanospheres as a novel matrix for the quantitative detection and enrichment of small biomolecules by LDI-MS. The nanostructure showed advantages of low background, wide application range, high sensitivity, super high salt and protein tolerance, and good stability. The research opens up possibilities for designing more efficient nanostructure matrices and exploring the application of LDI-MS in various fields.
Article
Chemistry, Analytical
Xian-Na Wang, Bin Li
Summary: Gold nanoparticles/thiol-beta-cyclodextrin-functionalized TiO2 nanowires were prepared to enhance the performance of SALDI MS and MSI, showing superior detection sensitivity and repeatability.
ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Quan He, Cuirong Sun, Jian Liu, Yuanjiang Pan
Summary: This article discusses the role of matrix-assisted laser desorption/ionization mass spectrometry imaging in cancer drug development and therapy, emphasizing its importance in revealing drug distribution and metabolism.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Guanhua Zhang, Chunxia Ma, Qing He, Hongjing Dong, Li Cui, Lili Li, Lingyu Li, Yan Wang, Xiao Wang
Summary: In this study, Pt@MXene was synthesized as an ideal substrate for SALDI MS, and it showed excellent performance for the high-throughput detection of small-molecule natural products. It provided stronger signal intensity, wider molecular coverage, lower background interference, good salt and protein tolerance, good repeatability, and high detection sensitivity. The Pt@MXene substrate was also successfully used for quantifying target molecules in medicinal plants. This proposed method has great potential for wide applications.
Article
Agriculture, Multidisciplinary
Sophie E. Lellman, Christopher K. Reynolds, A. K. Barney Jones, Nick Taylor, Rainer Cramer
Summary: This study introduces the use of liquid atmospheric pressure MALDI for detecting bovine tuberculosis, using a three-class prediction model and nasal swabs for sample collection, with a short sample preparation time. The results show comparable sensitivity, specificity, and overall accuracy to the traditional skin test, demonstrating the potential of this method as a single-visit diagnostic platform that provides results on the same day of sampling.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Editorial Material
Reproductive Biology
Pierre Comizzoli, Cecilia Luvoni, Michelle Kutzler
Article
Food Science & Technology
Andrea Massaro, Marco Bragolusi, Alessandra Tata, Carmela Zacometti, Stephane Lefevre, Aline Fregiere-Salomon, Jean-Louis Lafeuille, Giuseppe Sammarco, Ingrid Fiordaliso Candalino, Michele Suman, Roberto Piro
Summary: This study developed a non-targeted method for authenticating black pepper using near-infrared spectroscopy (NIR) and the LASSO method, and successfully validated its accuracy and stability. The method can classify and predict samples, and can be conveniently operated through an online application.
Article
Chemistry, Applied
Marco Bragolusi, Alessandra Tata, Andrea Massaro, Carmela Zacometti, Roberto Piro
Summary: Nutritional information on food labels can influence consumers' dietary choices. This study used near infrared spectroscopy to verify the accuracy of nutritional labels on 103 food products. The results showed that 35% of processed food products did not have nutritional labels, and some of the labeled values did not match the experimental data. Therefore, this study demonstrated the capability of near infrared spectroscopy in monitoring compliance with EU regulations and providing reference methods for further confirmation.
JOURNAL OF NEAR INFRARED SPECTROSCOPY
(2023)
Article
Microbiology
Alessandra Tata, Filippo Marzoli, Miriam Cordovana, Alessia Tiengo, Carmela Zacometti, Andrea Massaro, Lisa Barco, Simone Belluco, Roberto Piro
Summary: This study developed and validated a method to automatically serotype Legionella pneumophila and distinguish it from Legionella non-pneumophila using Fourier-transform infrared spectroscopy (FT-IR) and machine learning. Data validation strategies were employed to ensure consistent performance of the method across a large microbial population. The method achieved high accuracy on different test sets from European collections.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Food Science & Technology
Carmela Zacometti, Alessandra Tata, Andrea Massaro, Giorgia Riuzzi, Marco Bragolusi, Giulio Cozzi, Roberto Piro, Sara Khazzar, Gabriele Gerardi, Flaviana Gottardo, Severino Segato
Summary: The study aimed to assess the seasonal variation in VOCs in raw milk from three indoor feeding systems. VOC profiles were determined by GC after HS-SPME. The results showed that the feeding system did not affect VOCs, but the season had a significant impact. The outcomes suggested that VOCs could be used to trace raw milk for further mild processing.
Article
Veterinary Sciences
Andrea Massaro, Alessandra Tata, Ivana Pallante, Valentina Bertazzo, Massimo Bottazzari, Laura Paganini, Brunella Dall'Ava, Annalisa Stefani, Jeroen De Buck, Roberto Piro, Nicola Pozzato
Summary: This study investigated the metabolic changes associated with natural MAP infection in dairy cattle using NMR spectroscopy and routine blood chemistry. The results showed altered metabolic pathways, including increased tyrosine metabolism and enhanced phenylalanine, tyrosine, and tryptophan biosynthesis in MAP-infected cattle. Increased synthesis and degradation of ketone bodies were observed in both infected and infectious cattle. The fusion of multiple data sources proved to be useful in exploring these metabolic pathway changes and potentially diagnosing negative animals within paratuberculosis-infected herds.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Agriculture, Multidisciplinary
F. Marzoli, A. Tata, A. Massaro, M. Bragolusi, E. Passabi, S. Cappellozza, A. Saviane, L. Tassoni, R. Piro, S. Belluco
Summary: Silk farming in Italy has declined in the past due to competition with Asian markets and pesticide use, but it has potential for future development in the food sector. This study aimed to assess the nutritional composition and presence of hazards in silkworms. The study found that some samples did not meet the microbiological criteria for certain bacteria and fungi. Additionally, traces of fungicides and heavy metals were observed, and mycotoxins were detected in certain products. Overall, this study highlights the potential impact of environmental contamination on the safety of silkworms and their derivative products.
JOURNAL OF INSECTS AS FOOD AND FEED
(2023)
Article
Food Science & Technology
Filippo Marzoli, Alessandra Tata, Carmela Zacometti, Serena Malabusini, Costanza Jucker, Roberto Piro, Antonia Ricci, Simone Belluco
Summary: This study evaluated the microbiological and chemical stability of A. domesticus powders during one year of storage at room temperature. The results showed that the cricket powders had suitable microbiological stability, but exhibited a clear tendency toward rancidity in terms of chemical stability. Samples dried at 120°C and lyophilized never exceeded the limit set for B. cereus count in A. domesticus powder. Lyophilized samples also had the lowest levels of aldehydes responsible for rancid smell.
FRONTIERS IN SUSTAINABLE FOOD SYSTEMS
(2023)
Article
Chemistry, Analytical
Carmela Zacometti, Alessandra Tata, Roberto Stella, Stefania Leone, Ivana Pallante, Marianna Merenda, Salvatore Catania, Nicola Pozzato, Roberto Piro
Summary: In this study, ambient mass spectrometry (AMS) screening method was applied to analyze a suspected animal poisoning case. The results showed evidence of vegetable material intoxication and confirmed the presence of toxic substances using other analysis methods. This study demonstrates the potential of AMS to offer a rapid and efficient alternative for analyzing toxic substances in autopsy specimens from animals.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biochemical Research Methods
Carmela Zacometti, Andrea Massaro, Tommaso di Gioia, Stephane Lefevre, Aline Fregiere-Salomon, Jean-Louis Lafeuille, Ingrid Fiordaliso Candalino, Michele Suman, Roberto Piro, Alessandra Tata
Summary: The study utilized TD-DART-HRMS technique to analyze authentic and adulterated samples of black pepper and dried oregano, demonstrating the utility of this technique in establishing spice authenticity. Merging different datasets allowed retrieval of more comprehensive information compared to sole TD-(+)DART-HRMS spectra.
JOURNAL OF MASS SPECTROMETRY
(2023)
Article
Developmental Biology
Isa Mohammed Alkali, Martina Colombo, Alberto Maria Luciano, Wojciech Nizanski, Hiba Ali Hassan, Piotr Dziegiel, Gaia Cecilia Luvoni
Summary: The study aimed to optimize vitrification and in vitro culture conditions for improved viability of bovine ovarian tissue, and found that culture on agarose inserts maintained better follicle morphology and lower apoptosis compared to culture inserts.
Article
Reproductive Biology
Kelly Annes, Christina Ramires Ferreira, Roniele Santana Valente, Thamiris Vieira Marsico, Juliana Hayashi Tannura, Juliano Coelho da Silveira, Flavio Henrique Silva, Fernanda da Cruz Landim-Alvarenga, Fernando Silveira Mesquista, Mateus Jose Sudano
Summary: Each living organism is unique due to the specific lipid identity of its organelles, and the distribution of these molecules plays a role in cellular activity. Studying the lipid profiles of bovine embryos at the organelle level can provide valuable insights into key physiological processes during preimplantation development. This study aimed to characterize four organelles in vitro-produced bovine embryos and evaluate the lipid species associated with each organelle.
Article
Agriculture, Dairy & Animal Science
Aracelle Elisane Alves, Tathiana Ferguson Motheo, Maricy Ferreira Apparicio, Giuliano Queiroz Mostachio, Ricarda Maria dos Santos, Wilter Ricardo Russiano Vicente, Gaia Cecilia Luvoni
Summary: The aim of this study was to evaluate early embryonic development in bitches using sequential vaginal cytologies, single progesterone measurement, and single laparoscopic insemination. The results showed that the optimal insemination time can be accurately predicted 48 hours post-ovulation based on a single progesterone measurement, and using high-quality semen results in a higher number of embryos. This finding is important for dog owners facing logistical or economic limitations in accurately planning the breeding time.
ANIMAL REPRODUCTION
(2023)
