Article
Agriculture, Dairy & Animal Science
Guillaume Domain, Hiba Ali Hassan, Eline Wydooghe, Osvaldo Bogado Pascottini, Anders Johannisson, Jane M. Morrell, Wojciech Nizanski, Ann Van Soom
Summary: This study investigated the use of single layer centrifugation (SLC) to select the best-quality spermatozoa before freezing dog semen. The results showed that SLC was effective in separating spermatozoa based on their quality, resulting in better quality spermatozoa after thawing compared to standard centrifugation. However, spermatozoa from suboptimal semen quality did not survive freezing as well as those from optimal semen quality, even after using SLC. Although SLC allows for the isolation of high-quality spermatozoa and improves sperm sample quality after thawing, it does not improve the inherent freezing ability of suboptimal semen quality. Eighteen pups have been born so far using SLC-selected frozen-thawed semen, demonstrating the fertility of these selected spermatozoa.
Article
Agriculture, Dairy & Animal Science
N. C. Tyler, M. Namntu, M. Ciacciariello
Summary: This experiment found that dietary crude protein had no significant effect on sperm concentration in male broiler breeders, but high calcium feed resulted in significantly poorer sperm concentration at certain ages. Birds fed the recommended levels of crude protein and calcium for males had better fertilizing potential at a certain age.
Article
Reproductive Biology
Heming Sui, Mei Sheng, Haiqin Luo, Gang Liu, Fei Meng, Zubing Cao, Yunhai Zhang
Summary: Sperm cryopreservation maintains the diversities of porcine genetic resources and improves utilization efficiency of boar semen in artificial insemination practices, but the metabolic markers for boar semen freezability remain largely unknown. This study identified differential metabolites in sperm and seminal plasma between poor and good freezability semen from a Chinese native pig, and potential markers for semen freezability were screened. The results showed that there were significant differences in the metabolome between poor and good freezability semen samples in both sperm and seminal plasma.
Review
Agronomy
Yunhe Zong, Yunlei Li, Yanyan Sun, Gamal M. K. Mehaisen, Tianxiao Ma, Jilan Chen
Summary: Ex situ preservation is an important method for preserving chickens, and currently cryopreservation of semen is the only method available for gamete preservation. This review article summarizes the main factors affecting chicken sperm cryopreservation, the key points for optimizing the cryopreservation technology, as well as the mechanism underlying freezing damage and freezability. It is hoped that this review will serve as a reference for future investigations on poultry semen cryopreservation technology.
Article
Reproductive Biology
Wael A. Khalil, Mahmoud A. E. Hassan, Kandil A. A. Attia, Hassan A. El-Metwaly, Mostafa A. El-Harairy, Abdelaziz M. Sakr, Sameh A. Abdelnour
Summary: The study found that adding 3.5% nanoemulsion of grape seed oil to the freezing extender significantly improved the quality and freezability of buffalo semen. The nanoemulsion enhanced the membrane integrity, vitality, and progressive motility of sperm, while reducing oxidative damage and apoptosis markers. In vitro experiments demonstrated that the use of grape seed oil nanoemulsion increased the pregnancy rate.
Article
Reproductive Biology
Britta Hensel, Laura Pieper, Markus Jung, Martin Schulze
Summary: In recent years, research on antibiotic-free, low-temperature storage of boar semen has shown promising results in the face of antimicrobial resistance. This study aimed to evaluate the influence of boar age, breed, and season on the quality of boar semen preserved at 5°C. The results showed that storage temperature and season had some influence on semen quality, while age had a smaller effect.
Article
Veterinary Sciences
Joachim Otte, Jonathan Rushton, Elpidius Rukambile, Robyn G. Alders
Summary: Most village poultry-keeping households suffer from bird losses due to disease, but the impact on their total income is relatively small. Therefore, for the majority of households, investing in poultry may not be a high priority.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Reproductive Biology
Emma O'Brien, Clara Malo, Cristina Castano, Pedro Garcia-Casado, Adolfo Toledano-Diaz, Belen Martinez-Madrid, Heriberto Rodriguez-Martinez, Manuel Alvarez-Rodriguez, Julian Santiago-Moreno
Summary: This study investigated the presence and distribution of aquaglyceroporin 3 (AQP-3) in dromedary camel sperm, as well as its role in cryoinjury. The results showed that AQP-3 expression was not associated with the individual sperm response to freezing-thawing process. Additionally, sperm head morphometry was not a reliable predictor of cryosurvival.
Article
Agriculture, Dairy & Animal Science
Martina Colombo, Maria Giorgia Morselli, Giulia Franchi, Sabine Schafer-Somi, Gaia Cecilia Luvoni
Summary: The aim of this study was to evaluate the possibility of freezing dog semen after 24 or 48 hours of cooled transport to an equipped laboratory when semen collection was performed in the field. The results showed that there were no significant differences among the frozen groups, regardless of freezing time or extender used. However, motility at thawing decreased at 48 hours. Therefore, freezing after cooled transport could be a good option for long-term preservation of dog semen, especially if the transport can be organized within 24 hours.
Article
Agriculture, Dairy & Animal Science
Margarida Fernandes, Pablo Rodriguez Hernandez, Joao Simoes, Joao Pedro Barbas
Summary: The study revealed that the egg yolk-based semen extender performed better in cryopreserving semen from Merino rams. However, there were significant interactions between different semen extenders, semen processing, and collection months on sperm parameters. Further research is needed to address the causes of these interaction effects and improve semen extenders.
Article
Agriculture, Dairy & Animal Science
Mitchell Welch, Terence Zimazile Sibanda, Jessica De Souza Vilela, Manisha Kolakshyapati, Derek Schneider, Isabelle Ruhnke
Summary: Maintaining the health and welfare of laying hens is crucial for optimal productivity and consumer confidence. Free-range egg production systems face unique challenges, such as increased exposure to parasites and injury. This study explores the use of RFID and machine learning to predict individual hens' health status.
Article
Veterinary Sciences
Elham Shokri, Ali Haniloo, Mohammad Zibaei, Ali Pezeshki, Kamyar Mansori, Kensuke Taira
Summary: This study investigated the prevalence of Toxocara larvae in free-range broiler chickens in Zanjan Province, Iran, and found that some chicken meat and viscera were infected. Therefore, prevention and control measures, as well as public awareness of Toxocara infection, are crucial.
BMC VETERINARY RESEARCH
(2022)
Article
Agriculture, Dairy & Animal Science
Koray Tekin, Muhammed Enes Inanc, Dogukan Ozen, Beste Cil, Kemal Tuna Olgac, Burak Yilmaz, Umut Tasdemir, Purhan Barbaros Tuncer, Serhat Buyukleblebici, Ali Daskin, Ongun Uysal, Calogero Stelletta
Summary: Scientific attention to infrared technology has been increasing in the past decade, particularly in the field of andrology. This study aimed to use non-invasive infrared monitoring to investigate the relationship between reproductive temperature patterns and sperm quality in dogs. The results showed that factors such as body weight, testicular volume, ejaculation time, and temperature patterns can impact semen quality and freezability.
Review
Veterinary Sciences
Claire Bonnefous, Anne Collin, Laurence A. Guilloteau, Vanessa Guesdon, Christine Filliat, Sophie Rehault-Godbert, T. Bas Rodenburg, Frank A. M. Tuyttens, Laura Warin, Sanna Steenfeldt, Lisa Baldinger, Martina Re, Raffaella Ponzio, Anna Zuliani, Pietro Venezia, Minna Vaere, Patricia Parrott, Keith Walley, Jarkko K. Niemi, Christine Leterrier
Summary: This article explores welfare challenges and solutions in free-range and organic production systems, suggesting methods to improve animals' immune system and treat diseases through nutritional means and preventive methods, as well as the use of phytotherapy and aromatherapy.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Andrology
Abhishek Kumar, Subrata Kumar Ghosh, Rahul Katiyar, Amare Eshetu Gemeda, Rupali Rautela, Amarjeet Bisla, Neeraj Srivastava, Sanjeev Kumar Bhure, Huidrom Lakshmi Devi, Vikash Chandra
Summary: This study investigated the effects of Mito TEMPO and acetovanillone on the freezability of buffalo spermatozoa. The results showed that the addition of these compounds, either individually or in combination, significantly improved post-thaw semen quality.
Article
Agriculture, Dairy & Animal Science
B. Bernal, C. Castano, M. C. Esteso, A. Toledano-Diaz, M. A. Dominguez-Gonzalez, M. G. Gil, A. Lopez-Sebastian, J. L. Campo, E. Blesbois, J. Santiago-Moreno
Summary: The study found no significant differences in fresh semen variables between breeds and breeders, but significant differences were found in post-thawed variables. Cockerels from one of the breeders exhibited higher sperm cryoresistance, possibly due to differences in management practices.
BRITISH POULTRY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
M. Marti-Colombas, M. J. Sanchez-Calabuig, C. Castano, A. Toledano-Diaz, E. Martinez-Nevado, A. Lopez-Goya, J. Santiago-Moreno
Summary: This study compared the effects of two cryoprotectants, DMA and DMSO, on the frozen-thawed sperm characteristics of black-footed penguins and gentoo penguins. The results showed that 8% DMSO was more effective for black-footed penguin sperm, while there were no significant differences in gentoo penguin samples. Additionally, black-footed penguin sperm was more sensitive to the freezing-thawing process compared to gentoo penguin sperm.
ANIMAL REPRODUCTION SCIENCE
(2022)
Review
Agriculture, Dairy & Animal Science
J. Santiago-Moreno, E. Blesbois
Summary: Over the last century, various reproductive biotechnologies have been developed to improve poultry breeding stocks and conserve genetic diversity. These technologies include artificial insemination, semen storage, PGC methodologies, and gonad tissue storage and transplantation. Combining these technologies allows for the conservation and rapid reconstitution of poultry populations.
Article
Cell Biology
Paula Boveda Gomez, Rosario Velazquez, Lucia Martinez-Fresneda, Octavio Mejia, Marta Oteo, Adolfo Toledano-Diaz, Cristina Castano, Milagros Cristina Esteso, Rodolfo Ungerfeld, Antonio Lopez-Sebastian, Julian Santiago-Moreno
Summary: This study examined the influence of induced changes in prolactin secretion on sperm cryoresistance in ibex and mouflon. The findings suggest that high levels of blood plasma prolactin negatively affect the cryoresistance of sperm in these animals.
BIOPRESERVATION AND BIOBANKING
(2023)
Article
Agriculture, Dairy & Animal Science
Rodolfo Ungerfeld, Julia Giriboni, Adolfo Toledano-Diaz, Madeleine Guerrero, Julian Santiago-Moreno
Summary: Transrectal ultrasonic-guided massage of the accessory sex glands (TUMASG) is a technique used for semen collection. Administering carbetocin before TUMASG can shorten the collection time and decrease electrical stimuli, but it has minimal effects on semen quality.
REPRODUCTION IN DOMESTIC ANIMALS
(2023)
Article
Reproductive Biology
Emma O'Brien, Clara Malo, Cristina Castano, Pedro Garcia-Casado, Adolfo Toledano-Diaz, Belen Martinez-Madrid, Heriberto Rodriguez-Martinez, Manuel Alvarez-Rodriguez, Julian Santiago-Moreno
Summary: This study investigated the presence and distribution of aquaglyceroporin 3 (AQP-3) in dromedary camel sperm, as well as its role in cryoinjury. The results showed that AQP-3 expression was not associated with the individual sperm response to freezing-thawing process. Additionally, sperm head morphometry was not a reliable predictor of cryosurvival.
Article
Veterinary Sciences
Julia Giriboni, Eva Martinez-Nevado, Juncal Garcia, Rosario Velazquez, Adolfo Toledano-Diaz, Rodolfo Ungerfeld, Julian Santiago-Moreno
Summary: Developing strategies for population control is crucial in both captive wildlife and production animals. Immunization against GnRH is an effective method for reduction of testosterone and sperm quality, but its effectiveness varies with species and treatment repetition.
Article
Veterinary Sciences
Patricia Peris-Frau, Julia Benito-Blanco, Eva Martinez-Nevado, Adolfo Toledano-Diaz, Cristina Castano, Rosario Velazquez, Belen Pequeno, Belen Martinez-Madrid, Milagros C. Esteso, Julian Santiago-Moreno
Summary: This study investigated the cryopreservation of testicular tissue using slow freezing and vitrification methods in diverse wild species. The results showed that the effectiveness of each method varied depending on the species and cell type.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Veterinary Sciences
Belen Pequeno, Cristina Castano, Manuel Alvarez-Rodriguez, Paula Boveda, Maria Gemma Millan de la Blanca, Adolfo Toledano-Diaz, Diego Andres Galarza, Heriberto Rodriguez-Martinez, Belen Martinez-Madrid, Julian Santiago-Moreno
Summary: The study aimed to investigate the influence of osmotic changes on the location of aquaporins (AQPs) in spermatozoa during freeze-thawing process. Individual variability in terms of freezeability was observed among rams and their ejaculates. AQP3 expression in the mid- and principal piece of spermatozoa was found to be higher in frozen-thawed samples compared to fresh specimens in the good freezability group, but such differences were not detected in the poor freezability group. AQP3 expression is associated with sperm cryotolerance and can be used as a biomarker.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Review
Agriculture, Dairy & Animal Science
J. Santiago-Moreno, A. Toledano-Diaz, C. Castano, R. Velazquez, P. Boveda, E. O'Brien, P. Peris-Frau, B. Pequeno, B. Martinez-Madrid, M. C. Esteso
Summary: Reproductive technologies can help protect wild ruminant species from extinction and increase the number of animals. Efforts have focused on preserving sperm, oocytes, and embryos. Cryopreservation of sperm is the most commonly used and cheapest method for germplasm storage. New reproductive biotechnologies, such as ultra-rapid freezing techniques, have been developed. However, the success of artificial insemination using frozen-thawed sperm is not always satisfactory in wild small ruminants. Epididymal sperm shows higher cryoresistance than ejaculated sperm, and the seasonal variation in testosterone and prolactin levels is involved in sperm cryoresistance.
Article
Biology
Adrian Martin, Cristina Castano, Emma O'Brien, Adolfo Toledano-Diaz, Rafael Guerra, Felix Gomez-Guillamon, Julian Santiago-Moreno
Summary: This study examines the effect of equilibration time with extender on the variables of ultra-rapidly frozen-thawed sperm from wild ruminants. The results show that equilibration improves the quality and viability of sperm. Epididymal sperm demonstrates greater cryoresistance, while ejaculated sperm shows no dimensional changes before and after equilibration.
Article
Developmental Biology
B. Bernal, A. Behnamifar, C. Alvarez-Rodriguez, A. Toledano-Diaz, C. Castano, R. Velazquez, M. G. Gil, A. Gutierrez-Adan, Henri Woelders, E. Blesbois, J. Santiago-Moreno
Summary: This study aimed to evaluate the chromatin compaction of rooster spermatozoa along the male reproductive tract and to study the vas deferens lining cells potentially involved in sperm maturation. The results showed that the transit though the vas deferens resulted in a higher percentage of compacted chromatin spermatozoa in the ejaculate.
REPRODUCTION FERTILITY AND DEVELOPMENT
(2022)
