Article
Reproductive Biology
Seon Ah Ryu, Song Baek, Keun Cheon Kim, Eun Song Lee, Seung Tae Lee
Summary: The presence of cumulus cells is not essential for the completion of cytoplasmic maturation in oocytes after nuclear maturation. Removal of cumulus cells from porcine medium antral follicles (MAFs)-derived cumulus-oocyte complexes (COCs) after nuclear maturation results in significant improvements in cytoplasmic maturation and preimplantation development. The cumulus cells do not play a relevant role in cytoplasmic maturation of immature oocytes.
Article
Agriculture, Dairy & Animal Science
Yue Han, Xinglin Qu, Xuan Chen, Yanqiu Lv, Yuyang Zhang, Yi Jin
Summary: This study investigates the effect of porcine follicular fluid exosomes on cumulus expansion and oocyte maturation. The results suggest that porcine FF-Exo play a role in oocyte nuclear maturation but do not significantly affect cumulus expansion or mitochondrial membrane potential.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Bereket Molla Tanga, Xun Fang, Seonggyu Bang, Chaerim Seo, Heejae Kang, Dabin Cha, Ahmad Yar Qamar, Joohyun Shim, Kimyung Choi, Islam M. Saadeldin, Sanghoon Lee, Jongki Cho
Summary: Combining rolipram and cilostamide as cAMP modulators can greatly enhance the developmental competence of in vitro matured porcine oocytes. Pre-treatment for four hours was found to maintain optimal gap junction communication. The combined treatment group showed higher glutathione levels, lower reactive oxygen species levels, and a higher maturation rate compared to the control and single treatment groups. Two-phase in vitro maturation resulted in higher cleavage and blastocyst formation rates in parthenogenetic activation and somatic cell nuclear transfer embryos.
SCIENTIFIC REPORTS
(2023)
Article
Agriculture, Dairy & Animal Science
Muhammad Rosyid Ridlo, Eui Hyun Kim, Anukul Taweechaipaisankul, Byeong Chun Lee, Geon A. Kim
Summary: Successful attenuation of ER stress signaling benefits in vitro embryonal development, with adiponectin treatment enhancing the developmental capacity of porcine embryos by reducing ER stress and apoptosis, improving cleavage rates, blastocyst formation rates, and total cell numbers.
Article
Cell Biology
Huage Liu, Dan Zhou, Cong Liu, Qingrui Zhuan, Yan Luo, Xianhong Mo, Xiangwei Fu, Yunpeng Hou
Summary: The study showed that the Calcium-Sensing Receptor (CASR) plays a role in FSH-stimulated porcine cumulus expansion, with CASR agonist promoting cumulus expansion and CASR inhibitor inhibiting it. In addition, the CASR agonist upregulated expression of hyaluronan acid synthase 2 (HAS2), while CASR inhibitor downregulated expression of HAS2, prostaglandin-endoperoxide synthase 2 (PTGS2), and tumor necrosis factor a-induced protein 6 (TNFAIP6).
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Nico G. Menjivar, Ahmed Gad, Samuel Gebremedhn, Soham Ghosh, Dawit Tesfaye
Summary: Climate change-induced global warming leads to hyperthermia in dairy and beef animals, impacting reproductive function. Extracellular vesicles (EVs) play a crucial role in regulating processes like folliculogenesis and signaling pathways. In this study, granulosa cell-derived EVs (GC-EVs) were tested for their ability to improve bovine oocytes' thermotolerance to heat stress (HS) during in vitro maturation (IVM). Results show that thermally stressed GCs release EVs (S-EVs) that protect oocytes from HS by reducing ROS accumulation, improving mitochondrial function, and suppressing stress-associated gene expression. Additionally, GC-EVs have a carryover effect on oocyte maturation and development to the blastocyst stage, enhancing viability.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Cell Biology
G. D. Catandi, D. R. Bresnahan, S. O. Peters, K. J. Fresa, L. J. Maclellan, C. D. Broeckling, E. M. Carnevale
Summary: Many age-related changes in metabolite abundance suggest impaired mitochondrial metabolic function and oxidative stress in oocytes and follicular cells. The results also indicate impaired transfer of carbohydrate and free fatty acid substrates from cumulus cells to the oocytes of old mares, potentially related to disruption of transzonal projections between the cell types. The identification of age-associated alterations in the abundance of specific metabolites and their correlations among cells contribute to our understanding of follicular dysfunction with maternal aging.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jie Liu, Bin He, Chao Yin, Zhanglin Chang, Ruqian Zhao
Summary: This study found that heat exposure for 24 hours led to significantly impaired oocyte quality in pigs, characterized by disrupted cumulus expansion and extracellular matrix structure. Transcriptomic analysis revealed 1238 differential expression genes (DEGs) in the 24-hour heat-exposed group, indicating the impact of heat exposure on oocytes. Furthermore, comparison between the 24-hour and 4-hour heat-exposed groups showed downregulated DEGs mainly associated with extracellular matrix and cell proliferation.
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2021)
Article
Biochemistry & Molecular Biology
Alejandro de la Fuente, Charles Scoggin, Etta Bradecamp, Soledad Martin-Pelaez, Machteld van Heule, Mats Troedsson, Peter Daels, Stuart Meyers, Pouya Dini
Summary: This study aimed to elucidate the transcriptome dynamics of oocyte maturation by comparing transcriptomic changes during in vitro maturation in both oocytes and their surrounding cumulus cells. The results showed an association between oocyte maturation and pathways relating to nuclear maturation in oocytes and GTPase activity, extracellular matrix organization, and collagen trimers in cumulus cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Tien Dat Nguyen, Honghui Li, Yiquan Zhuang, Bowei Chen, Keiji Kinoshita, Muhammad Ameen Jamal, Kaixiang Xu, Jianxiong Guo, Deling Jiao, Kumiko Tanabe, Yunfang Wei, Zhuo Li, Wenming Cheng, Yubo Qing, Hong-Ye Zhao, Hong-Jiang Wei
Summary: This study successfully established an Asian elephant's fibroblast cell lines and performed interspecies somatic cell nuclear transfer with porcine oocytes. The reconstructed embryos showed in vitro development but failed to develop in vivo. Further investigation is needed to understand the reasons behind the in vivo developmental failure.
ANIMAL BIOTECHNOLOGY
(2023)
Article
Endocrinology & Metabolism
Qigui Xie, Yaxin Xing, Jianhong Zhou, Ling Wang, Jie Wu, Ri-Cheng Chian
Summary: Supplementation of lysophosphatidic acid in culture medium significantly improves in vitro human oocyte maturation. RNA sequencing identified potential pathways, such as TNF signaling and cell cycle, that may be associated with the enhanced oocyte maturation.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Fabiana de Andrade Melo-Sterza, Ralf Poehland
Summary: Lipids play a crucial role in the initial development of embryos, but excessive lipid droplet formation may be harmful to embryo cryotolerance. Additionally, increased lipid accumulation in in vitro produced embryos may be a response to stress, and there are still many questions regarding embryo lipid metabolism and developmental potential.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, Research & Experimental
Jianhua Li, Jing Chen, Tiecheng Sun, Shuiwen Zhang, Tingting Jiao, Ri-Cheng Chian, Youzhu Li, Ye Xu
Summary: In vitro oocyte maturation (IVM) does not affect the quality of embryos and the aneuploidy rate of embryos. The aneuploidy rate of embryos increases with maternal age, especially over 35 years old, whether in IVF or IVM group. Embryos with poor quality, especially grade III embryos, are recommended to be tested by PGS.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Genetics & Heredity
Megan Lim, Hannah M. Brown, Ryan D. Rose, Jeremy G. Thompson, Kylie R. Dunning
Summary: Hemoglobin and 2,3-BPG may have a role in the maturation of cumulus-oocyte complexes (COCs). The study found that in vitro maturation (IVM) increases the expression of Bpgm within COCs compared to in vivo. Decreased oxygen concentration and the addition of hemoglobin affected Bpgm expression, but not to the levels observed in vivo.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2021)
Article
Reproductive Biology
Xiaotong Luo, Xuan Chen, Yanqiu Lv, Yue Han, Xinglin Qu, Yuyang Zhang, Xin Li, Yongsheng Yu, Yi Jin
Summary: This study compared miRNA expression in porcine cumulus cells before and after oocyte maturation using RNA-seq technology and found that miR-101 regulates oocyte maturation by targeting HAS2.