Article
Reproductive Biology
Guo Chen, Li Ren, Zhanglin Chang, Yuting Zhao, Yanwen Zhang, Dong Xia, Ruqian Zhao, Bin He
Summary: High glucose concentration-induced lysine acetylation participates in the regulation of boar spermatozoa motility and acrosome integrity during preservation.
Article
Veterinary Sciences
Elisa Giaretta, Beatrice Mislei, Felipe Martinez-Pastor, Salvatore Nesci, Marcella Spinaci, Giovanna Galeati, Chiara Nerozzi, Gaetano Mari, Carlo Tamanini, Diego Bucci
Summary: This study aimed to investigate the role of mitochondria in horse sperm motility and ROS production by incubating spermatozoa with specific inhibitors. The results showed that disruption of mitochondrial integrity led to an increase in mitochondrial ROS production, which negatively affected sperm function and survival.
RESEARCH IN VETERINARY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Anna Zmudzinska, Jerzy Wisniewski, Piotr Mlynarz, Beata Olejnik, Marzena Mogielnicka-Brzozowska
Summary: Increased male age is associated with a reduction in semen quality. This study investigated the relationship between the functional quality and proteome of epididymal spermatozoa in dogs of different ages. The results showed age-dependent variations in sperm proteome composition, which may be potential biomarkers for aging.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Miao Tian, Qing Li, Yang Liu, Peng Zheng, Danyu Li, Yanpeng Zhao, Bing Wang, Chenhao Li, Jing Wang, Peng Gao, Qing Tang, Xiaochun Zhang, Hong Wu
Summary: In this study, a new model of DL detection system was established to investigate the DL emission of Saccharomyces cerevisiae cells cultured in different glucose concentrations. The results showed that the DL emission had a strong correlation with mitochondrial morphology, OCR, and MMP. Therefore, DL may be an effective method to detect mitochondrial metabolism related disorders.
SCIENTIFIC REPORTS
(2022)
Article
Reproductive Biology
Hai Thanh Nguyen, Son Quang Do, Hiroshi Kobayashi, Takuya Wakai, Hiroaki Funahashi
Summary: The purpose of this study was to investigate the relationship between mitochondrial content of commercial frozen-thawed bull spermatozoa and motility. The results showed that MDCN and MC of frozen spermatozoa differ among sires, and are negatively correlated with HMMP and sperm motility parameters. It should be noted that the MDCN and MC of bull spermatozoa may not vary overall with the age of the sire, whereas those changes with age in some individuals and may affect sperm motility.
Article
Biochemistry & Molecular Biology
David F. Carrageta, Lais Freire-Brito, Barbara Guerra-Carvalho, Joao C. Ribeiro, Bruno S. Monteiro, Alberto Barros, Pedro F. Oliveira, Mariana P. Monteiro, Marco G. Alves
Summary: UCPs play a crucial role in regulating mitochondrial activity and ROS production. The expression and function of UCPs in human spermatozoa have been investigated, providing valuable insights into the mechanisms of male infertility.
Article
Agriculture, Dairy & Animal Science
Vitaly Denisenko, Irena Chistyakova, Natalia Volkova, Ludmila Volkova, Baylar Iolchiev, Tatyana Kuzmina
Summary: Progesterone affects the functional status of bovine spermatozoa by inducing capacitation and acrosome reactions. Prolactin and dibutyryl cyclic adenosine monophosphate can modulate these effects in the presence of progesterone.
Article
Biochemistry & Molecular Biology
Rossella Cannarella, Francesca Mancuso, Nunziata Barone, Iva Arato, Cinzia Lilli, Catia Bellucci, Marco Musmeci, Giovanni Luca, Sandro La Vignera, Rosita A. Condorelli, Aldo E. Calogero
Summary: This study evaluated the expression of the follicle-stimulating hormone receptor (FSHR) in human spermatozoa and its effects on sperm function. The results showed that FSHR is expressed in the post-acrosomal segment, neck, midpiece, and tail of human spermatozoa, with no difference in localization between patients and controls. Incubation with FSH increased FSHR expression in patients. Furthermore, FSH incubation had a negative effect on sperm motility and mitochondrial function in both patients and controls, but more severely in controls. FSH also activated the AKT(473) signaling pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Chiara Castellini, Mario Muselli, Antonio Parisi, Maria Totaro, Daniele Tienforti, Giuliana Cordeschi, Macro Giorgio Baroni, Mauro Maccarrone, Stefano Necozione, Sandro Francavilla, Arcangelo Barbonetti
Summary: This meta-analysis study found a weak negative association between urinary levels of BPA and sperm motility. The overall estimates of data produced by clinical studies indicate a clinically negligible association between urinary BPA concentrations and semen quality.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Fisheries
Takafumi Fujimoto, Takahisa Kaneyasu, Mitsuru Endoh, Yuya Kogame, Joanna Nynca, Andrzej Ciereszko, Eisuke Takahashi, Etsuro Yamaha, Kiyoshi Naruse, Katsutoshi Arai
Summary: The aim of this study was to evaluate the cryopreservation of masu salmon semen using a specific extender and to investigate the relationship between semen parameters and post-thaw sperm motility. The results showed that the suitability of semen for cryopreservation was affected by the condition of the parental fish.
Article
Multidisciplinary Sciences
Yanhe Zhao, Huafeng Wang, Caroline Wiesehoefer, Naman B. Shah, Evan Reetz, Jae Yeon Hwang, Xiaofang Huang, Tse-en Wang, Polina V. Lishko, Karen M. Davies, Gunther Wennemuth, Daniela Nicastro, Jean-Ju Chung
Summary: The study visualizes the native in-cell 3D structure and higher-order organization of the CatSper calcium channels using cryo-electron tomography. The authors discover that the CatSper channels form long zigzag rows along the sperm tail and interact with other molecules. Moreover, the loss of EFCAB9-CATSPER zeta alters the architecture and interactions of the channels, disrupting flagellar movement in sperm.
NATURE COMMUNICATIONS
(2022)
Review
Biochemistry & Molecular Biology
Chiara Castellini, Settimio D'Andrea, Giuliana Cordeschi, Maria Totaro, Antonio Parisi, Giovanna Di Emidio, Carla Tatone, Sandro Francavilla, Arcangelo Barbonetti
Summary: Recent studies suggest that glycolysis plays an important role in mammalian spermatozoa, challenging the dogma that mitochondria are the main source of energy supporting sperm motility. Dysfunctional mitochondria in human sperm are believed to be the main site of reactive oxygen species (ROS) generation, which can negatively impact sperm integrity and function through a complex relationship with oxidative stress. Despite the presence of antioxidant factors, human sperm are particularly sensitive to oxidative stress, contributing significantly to male factor infertility.
Article
Biochemistry & Molecular Biology
Mercedes Torres-Badia, David Martin-Hidalgo, Rebeca Serrano, Luis J. Garcia-Marin, Maria J. Bragado
Summary: This study compares the effects of BPA and its analog BPS on pig sperm, focusing on motility, intracellular signaling pathways, and functional parameters. The results show that BPS has a smaller and slower impact on sperm compared to BPA, but significantly affects mitochondrial activity at a concentration of 100 mu M. However, BPA leads to adverse effects on sperm motility, mitochondrial function, and intracellular oxidant production. These findings help explain the reduced sperm motility caused by BPA and suggest that the mechanisms triggered by BPS are different.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Nutrition & Dietetics
Hiroshi Irisawa, Takashi Mizushima
Summary: This study investigated the effects of nutritional status, body composition, and muscle strength on activities of daily living (ADL) improvement in patients with proximal femur fractures. The findings showed that good nutritional status and skeletal muscle quality were significantly associated with ADL improvement, while poor nutritional status and decreased muscle strength interfered with ADL recovery.
Article
Reproductive Biology
Renzheng Zhang, Yiwei Chen, Pengjia Bao, Fude Wu, Chunnian Liang, Xian Guo, Min Chu, Ping Yan
Summary: This study identified proteins related to freezing tolerance in yak spermatozoa through proteomic analysis. The results showed that proteins involved in metabolic processes were upregulated in high-motility spermatozoa after frozen-thawed. Many antioxidant enzymes were also found to be upregulated in high-motility spermatozoa. Furthermore, KEGG analysis revealed the enrichment of differentially expressed proteins in glycolysis/gluconeogenesis, PPAR signaling pathway, and Ras signaling pathway. The findings of this study elucidate the mechanisms through which cryopreservation affects the movement of yak spermatozoa and offer a novel basis for refining freezing techniques and modifying cryopreservation extender components.
Article
Reproductive Biology
Raffaella Leoci, Giulio Aiudi, Vincenzo Cicirelli, Linda Brent, Carmelo Iaria, Giovanni Michele Lacalandra
Article
Agriculture, Dairy & Animal Science
Salvatore Alonge, Monica Melandri, Raffaella Leoci, Giovanni M. Lacalandra, Michele Caira, Giulio G. Aiudi
Article
Agriculture, Dairy & Animal Science
Salvatore Alonge, Monica Melandri, Raffaella Leoci, Giovanni M. Lacalandra, Michele Caira, Giulio G. Aiudi
Article
Mycology
Maria Elena Dell'Aquila, Shafaq Asif, Letizia Temerario, Antonella Mastrorocco, Giuseppina Marzano, Nicola Antonio Martino, Giovanni Michele Lacalandra, Bernard A. J. Roelen, Augusto Carluccio, Domenico Robbe, Fiorenza Minervini
Summary: In this study, the effects of low levels of OTA exposure on oocyte maturation, fertilization, and embryo development were evaluated using a juvenile sheep model. It was found that OTA induced cytotoxic effects at micromolar concentrations, while impairing oocyte ability to prevent polyspermy and increasing blastocyst apoptosis at nanomolar concentrations.
MYCOTOXIN RESEARCH
(2021)
Article
Agriculture, Dairy & Animal Science
Vincenzo Cicirelli, Pasquale Debidda, Nicola Maggio, Michele Caira, Daniela Mrenoshki, Giulio G. Aiudi, Giovanni M. Lacalandra
Summary: The study demonstrated that spinal anaesthesia combined with intercostal nerve block using levobupivacaine significantly improved postoperative analgesia after unilateral mastectomy in cats compared to a constant-rate infusion of sufentanyl citrate.
Article
Agriculture, Dairy & Animal Science
Vincenzo Cicirelli, Pasquale Debidda, Nicola Maggio, Michele Caira, Giovanni M. Lacalandra, Giulio G. Aiudi
Summary: This study compared the efficacy of ultrasound-guided funicular block with ropivacaine and general anaesthesia in orchiectomy in dogs. The use of ultrasound-guided funicular block with ropivacaine was shown to provide better analgesic coverage and minimize post-surgical pain.
Article
Reproductive Biology
Salvatore Desantis, Giovanni Michele Lacalandra, Miguel Batista, Olga Amann, Dario Antonelli, Davide Monaco
Summary: The exposure of epididymal spermatozoa to seminal plasma affects the glycocalyx composition of dromedary camel ES, potentially enhancing assisted reproductive techniques. More comprehensive studies are needed to evaluate the fertilization capacity of seminal plasma-treated ES for their application in dromedary camel assisted reproduction technology.
Article
Agriculture, Dairy & Animal Science
Vincenzo Cicirelli, Francesco Macri, Simona Di Pietro, Raffaella Leoci, Giovanni Michele Lacalandra, Giulio Guido Aiudi
Summary: The study monitored immediate vascular alterations in chemically sterilized dogs using CEUS, showing that the hardening agent caused a drastic reduction in intratesticular blood flow. Further research is needed to apply this methodology to a wider range of subjects, including stray dogs.
Review
Agriculture, Dairy & Animal Science
Vincenzo Cicirelli, Matteo Burgio, Giovanni M. Lacalandra, Giulio G. Aiudi
Summary: This review focuses on the application of local and regional anaesthetic/analgesic techniques in canine ovariectomy. The use of multimodal analgesia has been proven effective in minimizing surgical responses and ensuring adequate intraoperative and postoperative pain relief. The routine use of multimodal analgesia is considered a useful alternative for pain management in canine ovariectomy.
Article
Cell Biology
Antonella Mastrorocco, Ludovica Cacopardo, Letizia Temerario, Nicola Antonio Martino, Federico Tridente, Annalisa Rizzo, Giovanni Michele Lacalandra, Domenico Robbe, Augusto Carluccio, Maria Elena Dell'Aquila
Summary: In conventional assisted reproductive technologies, oocytes are cultured in vitro under static conditions. This study investigated a millifluidic in vitro oocyte maturation (mIVM) system, integrated with 3D printed supports, which better mimicked the physiological in vivo environment. Computational fluid dynamics and oxygen convection-reaction-diffusion models were used to model the system. The results showed that dynamic mIVM significantly reduced the maturation rate of oocytes and led to impaired mitochondria distribution and activity. However, when using specific 3D printed supports with alginate filling, oocyte maturation and mitochondria pattern were preserved, and bioenergetic/oxidative status was improved.
Article
Agriculture, Dairy & Animal Science
Davide Monaco, Giovanni Michele Lacalandra, Zeeshan Ansar, Paolo Trerotoli, Brendan Patrick Mulligan, Taher Kamal Osman
Summary: The aim of this study was to evaluate the effect of seminal plasma incubation on dromedary camel epididymal sperm motility, as well as the effects of centrifugation, cushion fluid, and enzymatic reduction of viscosity on dromedary camel ejaculates. The results showed that seminal plasma incubation reduced sperm motility, while centrifugation improved kinematic parameters. Samples treated with Papain + E64 showed increased progressive spermatozoa and reduced non-progressive spermatozoa. The study demonstrates that cushioned centrifugation, combined with enzymatic reduction of viscosity, can improve the motility patterns of dromedary camel spermatozoa.
Article
Agriculture, Dairy & Animal Science
Letizia Temerario, Davide Monaco, Antonella Mastrorocco, Nicola Antonio Martino, Sandor Cseh, Giovanni Michele Lacalandra, Elena Ciani, Maria Elena Dell'Aquila
Summary: In recent years, local animal breeds have faced genetic erosion and extinction risk due to industry and market preferences. The Gentile di Puglia (GdP) sheep breed, native to Southern Italy, is at risk and needs conservation strategies. This study aimed to evaluate reproductive efficiency on a GdP farm and establish a cryopreservation protocol for immature oocytes, leading to in vitro embryo production and transfer. Clinical and biotechnological-assisted reproduction approaches can support the conservation of GdP and other local sheep breeds.
Article
Ecology
R. Leoci, M. Ruberti
Summary: Research is being conducted to identify new degradable compounds and safer formulations to reduce pollution and pesticide risks. The use and control of chemical compounds vary worldwide, with some countries lacking proper means to control exposures. Although there is potential for increased use of certain compounds, precautions and guidelines from international bodies must be followed to prevent overuse and potential harm. More studies are needed to assess the effects of these compounds on reproductive functions of terrestrial and marine organisms.
INTERNATIONAL JOURNAL OF ECOLOGY & DEVELOPMENT
(2021)
Article
Veterinary Sciences
Salvatore Alonge, Giulio Guido Aiudi, Giovanni Michele Lacalandra, Raffaella Leoci, Monica Melandri
FRONTIERS IN VETERINARY SCIENCE
(2020)
Review
Agronomy
Raffaella Leoci, Marcello Ruberti
JOURNAL OF AGRICULTURE AND ENVIRONMENT FOR INTERNATIONAL DEVELOPMENT
(2020)
