Review
Agriculture, Dairy & Animal Science
J. L. Pate, C. H. K. Hughes
Summary: The corpus luteum plays a critical role in pregnancy in all mammals. However, the fate of the corpus luteum in ruminants depends on the presence of a functional uterus or signals from a developing embryo. Prostaglandin F2alpha, secreted by the uterus, is a key molecule that needs to be modified. The sensitivity of the corpus luteum to prostaglandin F2alpha can be influenced by internal mechanisms. Understanding the molecular changes that occur during different stages of luteal development and pregnancy can provide insight into the regulation of luteolytic capacity in ruminants.
Article
Agriculture, Dairy & Animal Science
Benjamin R. Crites, Sarah N. Carr, James C. Matthews, Phillip J. Bridges
Summary: Supplementing grazing cattle in selenium-deficient regions with an isomolar 1:1 mix of inorganic and organic selenium can increase early luteal phase progesterone levels, promoting embryonic development. The form of supplemental selenium affects the luteal transcriptome, regulating cholesterol biosynthesis and immune function in the corpus luteum.
JOURNAL OF ANIMAL SCIENCE
(2022)
Article
Reproductive Biology
D. V. Bonato, E. B. Ferreira, D. N. Gomes, F. G. C. Bonato, R. G. Droher, F. Morotti, M. M. Seneda
Summary: Two experiments were conducted in high milk-producing Holstein cows to evaluate the repeatability of antral follicle count (AFC) during ovulation synchronization protocol and investigate the follicular dynamics and Doppler ultrasonography. The results showed that AFC has high repeatability and cows with low AFC exhibited larger preovulatory follicles, greater perfusion in corpus luteum, and higher progesterone levels.
Article
Agriculture, Dairy & Animal Science
Thiago O. Cunha, Leah R. Statz, Rafael R. Domingues, Joao Paulo N. Andrade, Milo C. Wiltbank, Joao Paulo N. Martins
Summary: The objective of this study was to investigate the effects of inducing accessory corpus luteum on follicular and luteal dynamics in lactating Holstein cows. The results showed that inducing accessory corpus luteum not only altered the dynamics of follicles and corpus luteum but also deferred and prolonged the process of luteolysis.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Cecily Bishop, Vimal Selvaraj, David H. Townson, Joy L. Pate, Milo C. Wiltbank
Summary: The corpus luteum (CL) forms from the cellular remnants of the follicle and produces progesterone required for successful pregnancy. The CL and the embryo have a symbiotic relationship, both relying on each other for survival. The Northeast Multistate Project has contributed to our understanding of the regulation and function of the CL in cattle, providing valuable insights for improving fertility.
JOURNAL OF ANIMAL SCIENCE
(2022)
Article
Reproductive Biology
Megan A. Mezera, Wenli Li, Lihe Liu, Rina Meidan, Francisco Penagaricano, Milo C. Wiltbank
Summary: Research suggests that early subluteolytic PGF pulses impact the luteal transcriptome during natural luteolysis, though not by directly affecting steroidogenesis or inhibiting cellular function. Instead, these early pulses mainly alter pathways related to local steroid and glutathione metabolism.
BIOLOGY OF REPRODUCTION
(2021)
Article
Multidisciplinary Sciences
Yi-Fan Jiang, Pin-Huan Yu, Yovita Permata Budi, Chih-Hsien Chiu, Chi-Yu Fu
Summary: This study investigated the dynamics of mitochondria in mammalian ovaries during folliculogenesis and luteal formation. The results showed that mitochondrial networks shifted from branched structures in follicles to spherical and tubular mitochondria in large luteal cells. Additionally, mitochondrial diameter and volume increased from folliculogenesis to luteal formation, indicating mitochondrial enlargement as an event during these processes. Modulation of mitochondrial morphology and respiratory enzyme expressions may be related to tissue remodeling during luteal formation.
SCIENTIFIC REPORTS
(2021)
Article
Agriculture, Dairy & Animal Science
Samuel R. B. Couto, Yuri B. Guerson, Nicolas M. P. M. Caparelli, Joao Paulo N. Andrade, Julio C. F. Jacob, Rondineli P. Barbero, Marco R. B. Mello
Summary: The present study aimed to assess the impact of different strategies for increasing serum progesterone levels on luteal morphology and function in bovine females, as well as the effects on pregnancy rate and gestational loss in Nelore cows subjected to timed artificial insemination. The results showed that long-acting injectable P4 supplementation or GnRH administration after artificial insemination can improve luteal vascularization and increase pregnancy rates.
REPRODUCTION IN DOMESTIC ANIMALS
(2023)
Article
Agriculture, Dairy & Animal Science
Vikram Ramesh, Laishram S. Devi, Vivek Joshi, Moonmoon Mech, Kobu Khate, Meraj H. Khan
Summary: The study evaluated follicular dynamics and hormone concentrations during the oestrous cycle in Mithun cows. The results showed that Mithun cows primarily have three follicular waves and ovulation occurs approximately 10.5 hours after the end of oestrus.
REPRODUCTION IN DOMESTIC ANIMALS
(2022)
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Food Science & Technology
Dainan Yu, Xun Jiang, Wenjing Ge, Bo Qiao, Dalei Zhang, Hui Liu, Haibin Kuang
Summary: This study found that acrylamide exposure during early- and mid-pregnancy had negative effects on luteal function, including decreased levels of serum progesterone and estradiol, reduced numbers and relative areas of ovarian corpora lutea, dysregulation of ovarian angiogenesis, increased oxidative stress, and induced apoptosis of ovarian cells.
FOOD AND CHEMICAL TOXICOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Giuseppina Basini, Simona Bussolati, Simone Bertini, Fausto Quintavalla, Francesca Grasselli
Summary: The study identified that triclosan disrupts cellular function, particularly interfering with hormone production and cell proliferation, suggesting a critical evaluation of its effects.
Article
Biochemistry & Molecular Biology
Piotr Kaczynski, Monika Baryla, Ewelina Goryszewska, Agnieszka Waclawik
Summary: Intrauterine infusions of E2 can alter the expression of genes involved in CL function, potentially regulating DNA methylation as a physiological mechanism controlling luteal gene expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Agriculture, Dairy & Animal Science
Maria Camila Lopez-Duarte, Camila Londono, Sergio Lasso, Daniella Heredia, Morgan Brown, Nicolas DiLorenzo, Ky Pohler, Angela Gonella
Summary: During cell death, DNA is fragmented and enters the bloodstream as cell-free DNA (cfDNA). We hypothesized that cfDNA concentrations would increase when inducing luteolysis through the use of a prostaglandin F-2α (PGF2α) analog. The results showed a significant increase in cfDNA concentrations after luteolysis induction, indicating that cfDNA could be used as a biomarker for luteolysis in plasma.
REPRODUCTION IN DOMESTIC ANIMALS
(2023)
Article
Agriculture, Dairy & Animal Science
Ana Amaral, Carina Fernandes, Maria Rosa Rebordao, Anna Szostek-Mioduchowska, Karolina Lukasik, Pedro Pinto-Bravo, Luis Telo da Gama, Dariusz Jan Skarzynski, Graca Ferreira-Dias
Summary: The development of equine endometrosis can be slowed down by inhibiting the pro-fibrotic effects of myeloperoxidase using the inhibitor ABAH. Metallopeptidase-2 might be involved in an acute response to myeloperoxidase in the mid-luteal phase, while MMP-9 might be implicated in a prolonged exposition to myeloperoxidase in the follicular phase.
Article
Biochemistry & Molecular Biology
Ana Amaral, Carina Fernandes, Anna Szostek-Mioduchowska, Maria Rosa Rebordao, Dariusz Jan Skarzynski, Graca Ferreira-Dias
Summary: The research indicates that Noscapine has a potential inhibitory effect on equine endometrosis by reducing collagen synthesis, suggesting it could be a new therapeutic approach in the future.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Malgorzata J. McEvoy, Emilia Sinderewicz, Leo Creedon, Marion McAfee, Agnieszka W. Jonczyk, Katarzyna K. Piotrowska-Tomala, Dariusz J. Skarzynski
Summary: This study investigates newly discovered mechanisms of atresia and cell death processes in bovine ovarian follicles, finding that necroptosis is an alternative mechanism responsible for the death of bovine granulosa and theca cells. Analysis of mRNA expression of RIPK1 and RIPK3 supports this conclusion, highlighting the importance of both apoptosis and necroptosis in follicular cell death.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agriculture, Dairy & Animal Science
Pedro Pinto-Bravo, Maria Rosa Rebordao, Ana Amaral, Carina Fernandes, Antonio Galvao, Elisabete Silva, Pedro Pessa-Santos, Graca Alexandre-Pires, Rosario P. Roberto da Costa, Dariusz J. Skarzynski, Graca Ferreira-Dias
Summary: The oviduct provides ideal conditions for fertilization and early embryonic development, with proper vascularization being essential for its physiological function. This study examined differences in microvascular density across different portions of the equine oviduct and found that angiogenic factors and their receptors are expressed in all studied regions, in line with microvascular patterns.
Article
Agriculture, Dairy & Animal Science
Tomasz Jasinski, Lukasz Zdrojkowski, Ewa Kautz, Edyta Juszczuk-Kubiak, Graca Ferreira-Dias, Malgorzata Domino
Summary: Endometritis is a significant factor that reduces mares' fertility. The NF-kappa B pathway may not play a role in regulating inflammatory responses in equine endometritis.
REPRODUCTION IN DOMESTIC ANIMALS
(2022)
Article
Veterinary Sciences
Katarzyna K. Piotrowska-Tomala, Agnieszka W. Jonczyk, Anna Z. Szostek-Mioduchowska, Ewelina Zebrowska, Graca Ferreira-Dias, Dariusz J. Skarzynski
Summary: In this study, we investigated the impact of prostaglandin E-2 (PGE-2) on the secretory function of equine corpus luteum (CL), comparing the effects of intra-CL injection and intrauterine administration. We also compared the effects of these administration methods on CL function using both PGE-2 and human chorionic gonadotropin (hCG) as luteotropic factors. The results demonstrated that PGE-2 application, regardless of the site of administration, supported equine CL function and led to differences in progesterone and PGE-2 concentrations in the blood plasma.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Barbara M. Socha, Piotr Socha, Anna Z. Szostek-Mioduchowska, Tomasz Nowak, Dariusz J. Skarzynski
Summary: This study aimed to evaluate and compare the mRNA expression and immunolocalization of PPARα, PPARδ, and PPARγ in the canine endometrium. The results showed that the expression levels of PPARs changed during cystic endometrial hyperplasia (CEH) and CEH-pyometra, suggesting their potential involvement in hormonal disturbances and inflammatory processes.
REPRODUCTION IN DOMESTIC ANIMALS
(2022)
Article
Agriculture, Dairy & Animal Science
Terttu Katila, Graca Ferreira-Dias
Summary: Our understanding of post breeding endometritis and susceptibility of mares has changed over the last 100 years. Initially, it was believed that bacterial infection in the uterus after mating was the cause, but it was later discovered that sperm induce neutrophilia, indicating that post breeding endometritis is a physiological defense mechanism. In susceptible mares, this defense mechanism can be prolonged, leading to chronic endometritis. This paper reviews the pathogenesis of endometritis and endometrosis.
Article
Biochemistry & Molecular Biology
Tomasz Jasinski, Lukasz Zdrojkowski, Graca Ferreira-Dias, Ewa Kautz, Edyta Juszczuk-Kubiak, Malgorzata Domino
Summary: Endometrosis, a common disease that affects mares' fertility, is associated with alterations in epithelial and stromal cells, as well as degeneration and fibrosis of the endometrium. The study reveals a potential involvement of the NF-kappa B pathway in the pathogenesis of endometrosis, showing differential transcription of profibrotic genes between different phases of the estrous cycle. Additionally, the transcription of estrogen and progesterone receptors is found to be decreased with the severity of fibrosis and degeneration of the endometrium in horses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Dairy & Animal Science
Joana Alpoim-Moreira, Carina Fernandes, Maria Rosa Rebordao, Ana Luisa Costa, Miguel Bliebernicht, Telmo Nunes, Anna Szostek-Mioduchowska, Dariusz J. Skarzynski, Graca Ferreira-Dias
Summary: This study aimed to identify possible blood biomarkers for endometrosis diagnosis and fertility assessment on mares. The results suggest that serum COL3 concentration might be considered as a potential aid for the diagnosis of endometrosis and fertility prognosis in the mare. In contrast, COL1 and hydroxyproline did not prove to be effective as biomarkers of endometrial fibrosis in this species.
Article
Agriculture, Dairy & Animal Science
Barbara Maria Socha, Piotr Lada, Agnieszka Walentyna Jonczyk, Anna Justyna Korzekwa, Dariusz Jan Skarzynski
Summary: The aim of this study was to determine the expression of PPAR isoforms in the bovine corpus luteum throughout the estrous cycle, and their involvement in PGF(2 alpha)-induced processes related to luteolysis. The results showed that the mRNA and protein expression of PPARs changes in the bovine corpus luteum throughout the estrous cycle and under the influence of PGF(2 alpha). PPAR gamma, among all examined isoforms, could be involved in the regulation of PGF(2 alpha)-induced luteolysis in cattle.
Article
Biochemistry & Molecular Biology
Ana Amaral, Nelio Cebola, Anna Szostek-Mioduchowska, Maria Rosa Rebordao, Pawel Kordowitzki, Dariusz Skarzynski, Graca Ferreira-Dias
Summary: This study aims to evaluate the inhibitory effect of noscapine on collagen type 1 (COL1) induced by myeloperoxidase. The results show that myeloperoxidase increases the transcription of COL1A2 gene and the abundance of COL1 protein, while noscapine is able to reduce this effect, with a time/estrous cycle phase-dependent manner (in follicular phase explants, at 24 h of treatment).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Veterinary Sciences
Junwei Li, Wenming Zhao, Jiaqiao Zhu, Huiming Ju, Ming Liang, Shuaibiao Wang, Shufang Chen, Graca Ferreira-Dias, Zongping Liu
Summary: This study aims to explore the activation of AMP-activated protein kinase (AMPK) in boar spermatozoa under oxidative stress. The results show that oxidative stress promotes the activation of AMPK, which is positively correlated with sperm quality and functionality. This sheds light on the mechanism of sperm resistance to oxidative stress and provides valuable information for selecting antioxidants to improve reproductive outcomes.
VETERINARY SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Junwei Li, Wenming Zhao, Jiaqiao Zhu, Shuaibiao Wang, Huiming Ju, Shufang Chen, Athina Basioura, Graca Ferreira-Dias, Zongping Liu
Summary: Temperature changes during semen transport greatly damage boar sperm quality and functionality by promoting sperm apoptosis. Heat shock proteins and AMP-activated protein kinase play important roles in boar sperm resistance to temperature changes.