Journal
REPRODUCTION FERTILITY AND DEVELOPMENT
Volume 25, Issue 6, Pages 955-965Publisher
CSIRO PUBLISHING
DOI: 10.1071/RD12180
Keywords
folliculogenesis; goat; hormone; ovary
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Funding
- National Council for Scientific and Technological Development (CNPq)
- Coordination for Improvement of Graduate Personnel (CAPES)
- Brazilian Innovation Agency (FINEP)
- Ceara State Foundation for the Support of Scientific and Technological Development (FUNCAP)
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The aim of the present study was to evaluate the effects of growth differentiation factor 9 (GDF-9) and FSH on the in vitro development of caprine preantral follicles cultured for 16 days. Ovarian fragments were cultured in alpha MEM+ (alpha-minimum essential medium, pH 7.2-7.4, 10 mu g mL(-1) insulin, 5.5 mu g mL(-1) transferrin, 5.0 ng mL(-1) selenium, 2 mM glutamine, 2 mM hypoxanthine and 1.25 mg mL(-1) bovine serum albumin) in the absence or presence of 200 ng mL(-1) GDF-9 and/or 50 ng mL(-1) FSH added during the first (Days 0-8) and/or second (Days 8-16) half of the culture period. Non-cultured and cultured fragments were processed for histological and ultrastructural analyses. After 16 days, all treatments using GDF-9 or FSH showed higher rates of follicular survival compared with alpha MEM+ alone. Compared with non-cultured control, sequential culture media containing GDF-9 and/or FSH significantly increased the percentage of developing follicles and follicle diameter. Moreover, a progressive increase in oocyte diameter was observed only with sequential culture medium containing GDF-9 until Day 8 followed by FSH (GDF-9/FSH) in the second half of the culture period. After 16 days of culture, ultrastructural analysis confirmed the integrity of follicles cultured in the presence of GDF-9/FSH. In conclusion, a dynamic medium containing GDF-9 and FSH (GDF-9/FSH) maintained follicular integrity and promoted activation of primordial follicles and growth during long-term in vitro culture of goat preantral follicles.
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