Article
Reproductive Biology
Erika Cristina Dos Santos, Aldcejam Martins da Fonseca Junior, Camila Bruna de Lima, Jessica Ispada, Joao Vitor Alcantara da Silva, Marcella Pecora Milazzotto
Summary: The study introduced a novel culture media ECS100 for bovine IVP embryos and compared its performance with conventional SOFaa. Lowering the concentration of ECS100 (ECS50) actually improved embryo production rates, with embryos cultured in ECS50 showing better developmental rates and physiological traits compared to those in ECS100.
Article
Obstetrics & Gynecology
Molly M. Quinn, Philip Marsh, Salustiano Ribeiro, Rhodel K. Simbulan, Cristina Hickman, Jorgen Berntsen, Mitchell P. Rosen
Summary: Embryo quality, morphokinetic parameters, and aneuploidy rates were found to be similar in sibling embryos cultured in distinct media systems from the time of gamete isolation.
HUMAN REPRODUCTION
(2022)
Article
Multidisciplinary Sciences
Fang Fang, Zili Li, Jiangyu Yu, Yuting Long, Qian Zhao, Xiaofang Ding, Li Wu, Shumin Shao, Ling Zhang, Wenpei Xiang
Summary: The study suggests that miRNAs secreted by human embryos in culture media could serve as potential biomarkers for non-invasive embryo selection in assisted reproductive technology. Embryos with successful pregnancy release different miRNA profiles compared to those with failed pregnancy, indicating their predictive potential in reproductive outcomes. Selected miRNAs like hsa-miR-26b-5p and hsa-miR-21-5p show promising results as potential biomarkers for reproductive outcomes.
JOURNAL OF ADVANCED RESEARCH
(2021)
Article
Multidisciplinary Sciences
Tim Pock, Katharina Schulte, Stefan Schlatt, Michele Boiani, Verena Nordhoff
Summary: The aim of this study was to investigate the effect of GM-CSF supplementation on the cell number and cell lineages in early pre-implantation mouse embryos. The results showed that GM-CSF increased total cell numbers in mouse embryos, but did not have the same effect in human ART media. Additionally, there was an abnormal expression of NANOG in trophectoderm cells in GM-CSF supplemented media.
Article
Genetics & Heredity
Andrea Abdala, Ibrahim Elkhatib, Asina Bayram, Ana Arnanz, Ahmed El-Damen, Laura Melado, Barbara Lawrenz, Carol Coughlan, Nicolas Garrido, Human M. Fatemi, Neelke De Munck
Summary: Under controlled in vitro conditions, euploidy rates and embryo development are comparable when embryos are cultured in CCM or SCM.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2021)
Article
Agriculture, Multidisciplinary
Selcuk Kodaz, Kamil Haliloglu, Arash Hossein Pour, Murat Aydin
Summary: In the study of in vitro regeneration protocol for orchardgrass, it was found that Chu medium with 12 mg/L dicamba was most suitable for callus induction, and MS medium with 8 mg/L 2,4-D showed the best regeneration efficiency.
TURKISH JOURNAL OF AGRICULTURE AND FORESTRY
(2021)
Article
Plant Sciences
Dongliang Xiong, Jaume Flexas
Summary: This study assessed the impact of N supply on leaf anatomical, biochemical, and photosynthetic features in tobacco, revealing that high N treatment led to enhanced photosynthesis due to increased CO2 diffusion conductance and photosynthetic biochemical capacity. The increase in gm in leaves treated with high N was related to changes in leaf anatomy, but the rapid response to N top-dressing could not be fully explained by leaf anatomical modifications.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Zoology
Bruce E. E. Dunn
Summary: A shell-less culture system for chick embryos from the blastoderm stage to hatching is described for the first time. The technique involves suspending the egg contents in polymethylpentene kitchen wrap and rotating them for a specific period of time. The embryos obtained using this method showed normal development, and the hatch rate was approximately 43%.
JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL AND INTEGRATIVE PHYSIOLOGY
(2023)
Article
Cell Biology
Fatma Uysal, Selda Kahveci, Gozde Sukur, Ozgur Cinar
Summary: The study revealed that different culture media compositions can significantly affect DNA methylation levels in mouse embryos and oocytes, with sequential media showing lower levels of DNA methylating enzymes and global DNA methylation compared to single step media and control groups. Further studies are needed to investigate human embryos and oocytes and explore the underlying molecular mechanisms, as abnormal DNA methylation can potentially lead to developmental abnormalities.
JOURNAL OF MOLECULAR HISTOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Yanrong Gu, Dong Yan, Minna Wu, Min Li, Puze Li, Jingjing Wang, Yahan Chang, Fan Yang, Shaojun Di, Shijun Ni, Mengjie Yang, Jieyu Liu
Summary: Through experiments, it was found that blood-supplemented medium can increase the diversity of bacterial communities, while beef powder and peptone can significantly alter the enriched bacterial community. Optimal diversity on agar requires a moderate density of bacterial colonies, with membrane transport significantly enriched in this density range, indicating more active metabolism.
Article
Agronomy
Marcin Rozewicz, Marta Wyzinska, Jerzy Grabinski
Summary: The level of cereal yields and quality are largely determined by crop management practices, genetic potential, and factors that may cause damage. Fungal diseases of cereals can significantly reduce yield, with factors such as weather conditions, crop rotation, soil tillage, and nitrogen fertilisation playing key roles in their occurrence. These diseases limit plant growth and development, ultimately affecting grain yield and quality.
Article
Zoology
Bruce E. E. Dunn
Summary: This article describes a method for culturing chicken embryos and egg contents outside of the eggshell until hatching. The cultured embryos were suspended in kitchen wrap supported by tripods. The hatch rate increased with the supplementation of calcium L-lactate hydrate.
JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL AND INTEGRATIVE PHYSIOLOGY
(2023)
Article
Horticulture
Mihail Kantor, Zafar Handoo, Camelia Kantor, Lynn Carta
Summary: Plant-parasitic nematodes are significant pests that cause substantial crop losses and economic damage. This review focuses on the top ten economically important regulated and emerging plant-parasitic nematodes, providing information on their diagnostics, morphology, and molecular features, as well as their distribution and classification.
Review
Biochemistry & Molecular Biology
Arsinoe C. Thomaidou, Panagiota Batsaki, Maria Adamaki, Maria Goulielmaki, Constantin N. Baxevanis, Vassilis Zoumpourlis, Sotirios P. Fortis
Summary: Head and neck cancers comprise a heterogeneous group of tumors with diverse etiologic factors. Certain miRNAs have been identified as potential biomarkers for the diagnosis and prognosis of HNC. Ongoing clinical trials aim to determine the utility of miRNAs in HNC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Somayeh Faghih, Zabihollah Zamani, Reza Fatahi, Mohammad Omidi
Summary: The study demonstrated that kaolin spray treatments have significant effects on early apple cultivars, improving fruit weight, length, hardness, and soluble solids content. Deficit irrigation reduced fruit weight and increased leaf proline content, while severe deficit irrigation enhanced the activity of nonenzymatic defense systems in apple trees. High kaolin concentration combined with severe deficit irrigation had the most impact on apple trees.
BIOLOGICAL RESEARCH
(2021)
Article
Cell & Tissue Engineering
James Spyrou, David K. Gardner, Alexandra J. Harvey
Article
Cell & Tissue Engineering
Jarmon G. Lees, Timothy S. Cliff, Amanda Gammilonghi, James G. Ryall, Stephen Dalton, David K. Gardner, Alexandra J. Harvey
STEM CELLS INTERNATIONAL
(2019)
Article
Cell & Tissue Engineering
Jarmon G. Lees, Anne M. Kong, Yi C. Chen, Priyadharshini Sivakumaran, Damian Hernandez, Alice Pebay, Alexandra J. Harvey, David K. Gardner, Shiang Y. Lim
STEM CELLS INTERNATIONAL
(2019)
Article
Obstetrics & Gynecology
Kathryn H. Gurner, Joseph J. Richardson, Alexandra J. Harvey, David K. Gardner
Summary: The study showed that VEGF-loaded silica supraparticles (V-SPs) can promote embryonic development in vitro, similar to the effect of free VEGF supplementation. This suggests that V-SPs could be a novel method for delivering therapeutic agents to support embryo development, potentially overcoming the limitations of VEGF stability in solution. Further investigation is needed to explore the clinical application of V-SPs in IVF.
HUMAN REPRODUCTION
(2021)
Article
Obstetrics & Gynecology
Kathryn H. Gurner, Jemma Evans, Jennifer C. Hutchison, Alexandra J. Harvey, David K. Gardner
Summary: This study suggests that lactate may play an important role in maternal-fetal dialogue, promoting endometrial receptivity and implantation. Further investigation of the role of lactate in infertile or compromised endometrium could potentially improve pregnancy success.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Article
Obstetrics & Gynecology
Thi Truong, Alexandra J. Harvey, David K. Gardner
Summary: This study investigated the effect of combined antioxidants on mouse fetal gene expression. The results showed that both in-vitro culture in the presence of 20% oxygen and vitrification of blastocysts significantly perturbed fetal liver and placental gene expression. However, supplementation with A3 reduced the number of differentially expressed genes and altered biological processes, establishing a more in-vivo-like gene expression profile, particularly in the placenta. Additionally, A3 supplementation significantly reduced the expression of genes associated with pre-eclampsia and intrauterine growth restriction.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Article
Obstetrics & Gynecology
David K. Gardner
Summary: By introducing more technologies in the ART laboratory, outcomes such as live birth rate, time to pregnancy, and automation can be improved. New technologies have the potential to assist more patients in becoming parents of healthy children in a shorter period of time.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Article
Obstetrics & Gynecology
Emma G. Whatley, Thi T. Truong, Dagmar Wilhelm, Alexandra J. Harvey, David K. Gardner
Summary: Exposure to the ketone beta-hydroxybutyrate (beta OHB) impairs preimplantation mouse embryo development, metabolism, epigenetics, and post-transfer viability, with significant effects seen at ketogenic diet (KD)-relevant serum concentrations. These findings suggest a potential detrimental impact on embryo development and viability due to maternal diets that elevate beta OHB levels.
HUMAN REPRODUCTION
(2022)
Article
Obstetrics & Gynecology
Emma G. Whatley, Thi T. Truong, Alexandra J. Harvey, David K. Gardner
Summary: Research question: Does the ketone acetoacetate (AcAc) alone, or combined with 13-hydroxybutyrate (13OHB), impact mouse embryo development, metabolism, histone acetylation and viability? The study found that exposure to high levels of ketone acetoacetate and 13-hydroxybutyrate affects metabolism and histone acetylation in embryos, and leads to abnormal fetal development.
REPRODUCTIVE BIOMEDICINE ONLINE
(2023)
Article
Obstetrics & Gynecology
David K. Gardner, Denny Sakkas
Summary: Our ability to maintain viable human embryos in vitro has greatly improved in the past 40 years, leading to higher implantation rates. The future development of embryo culture systems will benefit from new media formulations and more dynamic perfusion-based systems. Furthermore, the integration of artificial intelligence and biomarkers can predict embryo health and viability, improving the effectiveness and cost efficiency of assisted reproductive technology.
FERTILITY AND STERILITY
(2023)
Article
Multidisciplinary Sciences
Hanna J. McLennan, Adam J. Blanch, Samuel J. Wallace, Lesley J. Ritter, Shauna L. Heinrich, David K. Gardner, Kylie R. Dunning, Marty J. Gauvin, Allison K. Love, Jeremy G. Thompson
Summary: This study introduces a microfluidic chip that is designed and completely 3D-printed using two-photon polymerization (2PP). It consists of ten channels and ten retrievable cell sample seats, which can enhance cell-surface interactions at the nanoscale and support cell culture. This technology provides the precision needed for nanoliter fluidic devices constructed from multiple interlocking parts.
SCIENTIFIC REPORTS
(2023)
Article
Obstetrics & Gynecology
Emma G. Whatley, Thi T. Truong, Alexandra J. Harvey, David K. Gardner
Summary: In vitro exposure to ketone bodies fi-hydroxybutyrate (fiOHB) and acetoacetate (AcAc) has a sexually dimorphic impact on gene expression in the fetal liver and placenta of preimplantation mouse embryos.
REPRODUCTIVE BIOMEDICINE ONLINE
(2023)
Article
Obstetrics & Gynecology
Jennifer C. Hutchison, Jemma Evans, Tracey A. Edgell, Guiying Nie, David K. Gardner, Lois A. Salamonsen
Summary: The study found that obese infertile women have elevated levels of advanced glycation end-products (AGE) in their uterine environment. The detrimental effects of AGE on endometrial epithelial cells can be reduced with therapeutics and replicated in a more physiologically relevant primary model (organoids).
REPRODUCTIVE BIOMEDICINE ONLINE
(2023)
Meeting Abstract
Obstetrics & Gynecology
H. Elzeiny, F. Agtesta, F. Cattrall, J. Stevens, D. K. Gardner
HUMAN REPRODUCTION
(2022)
Article
Developmental Biology
Kathryn H. Gurner, Thi T. Truong, Alexandra J. Harvey, David K. Gardner
MOLECULAR HUMAN REPRODUCTION
(2020)