Article
Reproductive Biology
Joanna Kochan, Agnieszka Nowak, Barbara Kij, Karolina Fryc, Sylwia Prochowska, Wojciech Nizanski
Summary: This study compared different culture systems for cat embryos and found that culture in Primo Vision (R) dishes resulted in a higher proportion of blastocysts and hatching blastocysts compared to individual and group culture systems. Co-culture with cat or sheep embryos also accelerated the development of cat embryos.
Review
Medicine, Research & Experimental
Du Xiang, Yang Liu, Encheng Zhou, Yanfeng Wang
Summary: This article discusses the gradual decrease in ovarian reserve with age, the related diseases that may occur after the loss of estrogen protection postmenopausally, as well as the controversy surrounding PHRT and AO technology. The article also compares the advantages and disadvantages of different polymer biomaterials for use in follicle IVC, providing a theoretical basis for the development of mature AO technology.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Biotechnology & Applied Microbiology
Ramses Belda-Perez, Sonia Heras, Costanza Cimini, Jon Romero-Aguirregomezcorta, Luca Valbonetti, Alessia Colosimo, Bianca Maria Colosimo, Silvia Santoni, Barbara Barboni, Nicola Bernabo, Pilar Coy
Summary: With the increasing demand for assisted reproductive technologies, the use of 3D printing for in vitro fertilization studies has been explored. This study evaluated different 3D-printing processes and biomaterials for supporting bovine embryo development. The results showed that 3D-printed scaffolds made with extrusion-based processing and polycaprolactone (PCL) had the best outcomes in terms of embryo development.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Analytical
Cecilia Beatriz Figoli, Marcelo Garcea, Claudio Bisioli, Valeria Tafintseva, Volha Shapaval, Mariana Gomez Pena, Luz Gibbons, Fernando Althabe, Osvaldo Miguel Yantorno, Marcos Horton, Jurgen Schmitt, Peter Lasch, Achim Kohler, Alejandra Bosch
Summary: The study established a metabolomic-based approach using FTIR spectroscopy to identify the most competent embryos for transfer. Analysis revealed a highly heterogeneous nature of nonimplanted embryos' supernatant spectra, with approximately 60% exhibiting diverse metabolic fingerprints.
Article
Biochemistry & Molecular Biology
Mengxue Zheng, Jesus Cadenas, Susanne Elisabeth Pors, Tasnim Esa, Stine Gry Kristensen, Linn Salto Mamsen, Cristina Subiran Adrados, Claus Yding Andersen
Summary: This study aimed to optimize the culture conditions for murine preantral follicles to improve their growth and survival. The results showed that altering the alginate concentration, adding estradiol, and increasing follicle-stimulating hormone concentration can promote the growth and development of preantral follicles.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Helen M. Picton
Summary: Considerable progress has been made in the development of culture systems for in vitro growth and maturation of oocytes, but significant heterogeneity exists between different stages of follicle development and species. More research is needed to overcome technical challenges and establish multiphase culture approaches.
ANNUAL REVIEW OF ANIMAL BIOSCIENCES
(2022)
Article
Cell Biology
Jian Hua Li, Tie Cheng Sun, Shui Wen Zhang, Ting Ting Jiao, Yan Bin Cheng, Pan Dong, Ri-Cheng Chian, Ye Xu
Summary: The presence or ovulation of the dominant follicle does not significantly influence the developmental and implantation capacity of immature oocytes retrieved from small follicles.
Article
Obstetrics & Gynecology
Evelyn E. Telfer, Claus Yding Andersen
Summary: The cryopreservation of ovarian tissue is widely used to preserve fertility in high-risk females. Besides transplantation, in vitro maturation of oocytes from ovarian tissue can also be used to restore fertility safely in cases where transplantation is contraindicated.
FERTILITY AND STERILITY
(2021)
Article
Reproductive Biology
Jing Xu, Mary B. Zelinski
Summary: In vitro follicle development (IVFD) is a valuable model for understanding folliculogenesis, screening ovarian toxicity, and preserving fertility and species. This review discusses the culture systems used in IVFD across various species and the improvements made in follicle growth and oocyte maturation through optimization of culture conditions. The evaluation of oocyte quality following IVFD, including epigenetic and genetic profile, maturation, embryonic development, and pregnancy outcomes, is summarized. The review also highlights the limitations and knowledge gaps in IVFD for proper oocyte development. This information contributes to the goal of producing competent oocytes with genetic integrity for successful embryonic development and healthy offspring.
BIOLOGY OF REPRODUCTION
(2022)
Article
Biochemistry & Molecular Biology
Chiara Di Berardino, Alessia Peserico, Giulia Capacchietti, Martina Crociati, Maurizio Monaci, Umberto Tosi, Annunziata Mauro, Valentina Russo, Nicola Bernabo, Barbara Barboni
Summary: This study suggests that using eCG as an alternative to FSH stimulant helps to maximize the in vitro growth of ovine PA follicles in FBS-free medium, resulting in better follicular growth, antrum formation, steroidogenic activity, and expression of gap junction markers. Additionally, eCG also has a positive effect on the germinal compartment, leading to a higher incidence of meiotic competent oocytes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agriculture, Dairy & Animal Science
Kensuke Tomita, Toshimichi Ishii, Natsumi Endo, Tomomi Tanaka
Summary: This study aimed to investigate the effects of short-term supplementation with a high-energy diet on embryo production after ovum pick-up in Japanese Black cows. The high-energy diet group showed significantly higher plasma insulin concentrations, increased follicle count, and higher number of retrievable and cultivable oocytes compared to the control group. However, the high-energy diet had a negative impact on oocyte quality.
JOURNAL OF REPRODUCTION AND DEVELOPMENT
(2023)
Article
Medicine, General & Internal
Xia Hao, Amandine Anastacio, Kenny A. Rodriguez-Wallberg
Summary: Experimental study showed that secondary follicles isolated from ovaries of mice treated with gonadotropins could be cultured in vitro and mature, with development and maturation abilities similar to those from unstimulated controls.
JOURNAL OF CLINICAL MEDICINE
(2021)
Review
Physiology
Evelyn E. Telfer, Johanne Grosbois, Yvonne L. Odey, Roseanne Rosario, Richard A. Anderson
Summary: This review discusses the mechanisms and regulatory pathways that govern the processes of oocyte and follicle formation, growth, and ovulation, with a focus on human oocytes/follicles. It emphasizes the effects of aging on oocyte number and quality, as well as the clinical implications. Additionally, it outlines the current developments in culture systems for follicle growth and the emerging prospects for generating new oocytes from stem cells.
PHYSIOLOGICAL REVIEWS
(2023)
Article
Biochemistry & Molecular Biology
Inha Lee, So Hyun Ahn, Hye In Kim, Hye Won Baek, Yun Jeong Park, Heeyon Kim, Aminah Ibrahim Aljassim, Whan Shin, Chungsoon Ryu, Jeongmi Yoon, Jae Hoon Lee, Bo Hyon Yun, Seok Kyo Seo, Joo Hyun Park, Young Sik Choi, SiHyun Cho, Byung Seok Lee
Summary: Specific cytokines in human embryo culture media can be used to predict embryo quality and IVF outcomes. CCL15, CCL27, and CXCL-12 were found to be significantly elevated in the top-quality embryo group.
Article
Reproductive Biology
Anamaria-Cristina Herta, Nazli Akin, Katy Billooye, Laura Saucedo-Cuevas, Francesca Lolicato, Ingrid Segers, Ellen Anckaert, Johan Smitz
Summary: The study shows that oocytes from cultured mouse follicles can overcome complete denudation, restoring functional transzonal projections, with no significant differences in meiotic and developmental competence compared to those from intact cultured follicles. These findings provide good premises for developing successful strategies to rescue human oocyte competence in in vitro culture approaches.
BIOLOGY OF REPRODUCTION
(2021)
Article
Agriculture, Dairy & Animal Science
Nathalie Jiatsa Donfack, Kele Amaral Alves, Benner Geraldo Alves, Rebeca Magalhaes Pedrosa Rocha, Jamily Bezerra Bruno, Carlos H. Lobo, Marcelo Bertolini, Regiane Rodrigues dos Santos, Marilia de Oliveira Taumaturgo, Ramon da Silva Raposo, Jose Ricardo de Figueiredo, Johan Smitz, Ana Paula Ribeiro Rodrigues
REPRODUCTION IN DOMESTIC ANIMALS
(2019)
Article
Reproductive Biology
P. Conde, S. Morado, G. Alvarez, J. Smitz, T. Gentile, P. Cetica
Article
Reproductive Biology
Carolina Ortega-Hrepich, Panagiotis Drakopoulos, Claire Bourgain, Inge Van Vaerenbergh, Luis Guzman, Herman Tournaye, Johan Smitz, Michel De Vos
REPRODUCTIVE BIOLOGY
(2019)
Article
Genetics & Heredity
Flor Sanchez, Anh H. Le, Vu N. A. Ho, Sergio Romero, Heidi Van Ranst, Michel De Vos, Robert B. Gilchrist, Tuong M. Ho, Lan N. Vuong, Johan Smitz
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2019)
Correction
Oncology
Maria Desemparats Saenz-de-Juano, Elena Ivanova, Katy Billooye, Anamaria-Cristina Herta, Johan Smitz, Gavin Kelsey, Ellen Anckaert
CLINICAL EPIGENETICS
(2020)
Article
Oncology
Maria Desemparats Saenz-de-Juano, Elena Ivanova, Katy Billooye, Anamaria-Cristina Herta, Johan Smitz, Gavin Kelsey, Ellen Anckaert
CLINICAL EPIGENETICS
(2019)
Article
Reproductive Biology
Nazli Akin, Lucia von Mengden, Anamaria-Cristina Herta, Katy Billooye, Julia van Leersum, Berta Cava-Cami, Laura Saucedo-Cuevas, Fabio Klamt, Johan Smitz, Ellen Anckaert
Summary: The novel two-step IVM method CAPA-IVM has improved embryology outcomes by synchronizing oocyte nuclear and cytoplasmic maturation, although there is still a noticeable efficiency gap compared with traditional IVF. Glucose metabolism studies have revealed metabolic shortcomings within CAPA-IVM COCs, with specific alterations in mouse COC glucose metabolism characterized for the first time. Future optimization strategies for CAPA-IVM could focus on adjusting culture media energy substrate concentrations and/or implementing co-culture strategies.
BIOLOGY OF REPRODUCTION
(2021)
Article
Reproductive Biology
Anamaria-Cristina Herta, Nazli Akin, Katy Billooye, Laura Saucedo-Cuevas, Francesca Lolicato, Ingrid Segers, Ellen Anckaert, Johan Smitz
Summary: The study shows that oocytes from cultured mouse follicles can overcome complete denudation, restoring functional transzonal projections, with no significant differences in meiotic and developmental competence compared to those from intact cultured follicles. These findings provide good premises for developing successful strategies to rescue human oocyte competence in in vitro culture approaches.
BIOLOGY OF REPRODUCTION
(2021)
Article
Genetics & Heredity
Nazli Akin, Dulama Richani, Xiuhua Liao, Yiqing Zhao, Anamaria-Cristina Herta, Katy Billooye, William A. Stocker, David G. Mottershead, Craig A. Harrison, Johan Smitz, Ellen Anckaert, Robert B. Gilchrist
Summary: This study assessed the effect of adding novel TGF-beta superfamily proteins, cumulin and super-GDF beta, to the culture media in a biphasic IVM system. The results showed that cumulin and super-GDF9 significantly improved oocyte developmental competence in standard IVM, but had no effect in CAPA-IVM.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2022)
Article
Obstetrics & Gynecology
Ricassio S. Barberino, Thae Lanne B. G. Lins, Alane P. O. Monte, Bruna B. Gouveia, Daniela S. P. Campinho, Raimundo C. Palheta, Johan E. J. Smitz, Maria Helena T. Matos
Summary: This study evaluated the protective effect of melatonin before cyclophosphamide administration on ovarian function in mice. Pre-treatment with 20 mg/kg melatonin showed reductions in primordial follicle loss, apoptosis, and oxidative damage, as well as an increase in GSH concentrations. Melatonin exerted its protective effects through the MT1 receptor and PTEN/Akt/FOXO3a signaling pathway.
REPRODUCTIVE SCIENCES
(2022)
Article
Reproductive Biology
Anamaria-Cristina Herta, Lucia Mengden, Nazli Akin, Katy Billooye, Wim Coucke, Julia Leersum, Berta Cava-Cami, Laura Saucedo-Cuevas, Fabio Klamt, Johan Smitz, Ellen Anckaert
Summary: This study explored the glucose-related pathways in different follicle cell types and found altered metabolic patterns in in vitro-cultured mouse follicles, suggesting potential impacts on oocyte competence.
BIOLOGY OF REPRODUCTION
(2022)
Article
Biochemistry & Molecular Biology
Ricassio S. Barberino, Tais J. S. Macedo, Thae Lanne B. G. Lins, Vanuzia G. Menezes, Regina L. S. Silva, Alane P. O. Monte, Raimundo C. Palheta, Johan E. J. Smitz, Maria Helena T. Matos
Summary: This study investigated the expression of melatonin receptor type 1 (MT1) protein in sheep ovaries and evaluated the effects of melatonin on the survival and development of primordial follicles after in vitro culture. The results showed that 100 pg/ml melatonin could increase follicular survival and activation. Inhibition of the PI3K pathway resulted in increased apoptosis and decreased activation of primordial follicles. These findings suggest that 100 pg/ml melatonin can promote the survival and activation of primordial follicles through the PI3K signaling pathway in sheep ovarian tissue culture.
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2022)
Meeting Abstract
Obstetrics & Gynecology
A. C. Herta, N. Akin, K. Billooy, L. Saucedo-Cuevas, F. Lolicato, E. Anckaert, J. Smitz
HUMAN REPRODUCTION
(2019)
Article
Oncology
Alexandra S. Rashedi, Saskia F. de Roo, Lauren M. Ataman, Maxwell E. Edmonds, Adelino Amaral Silva, Anibal Scarella, Anna Horbaczewska, Antoinette Anazodo, Ayse Arvas, Bruno Ramalho de Carvalho, Cassio Sartorio, Catharina C. M. Beerendonk, Cesar Diaz-Garcia, Chang Suk Suh, Claudia Melo, Claus Yding Andersen, Eduardo Motta, Ellen M. Greenblatt, Ellen Van Moer, Elnaz Zand, Fernando M. Reis, Flor Sanchez, Guillermo Terrado, Jhenifer K. Rodrigues, Joao Marcos de Meneses e Silva, Johan Smitz, Jose Medrano, Jung Ryeol Lee, Katharina Winkler-Crepaz, Kristin Smith, Ligia Helena Ferreira Melo e Silva, Ludwig Wildt, Mahmoud Salama, Maria del Mar Andres, Maria T. Bourlon, Mario Vega, Mauricio Barbour Chehin, Michel De Vos, Mohamed Khrouf, Nao Suzuki, Osama Azmy, Paula Fontoura, Paulo Henrique Almeida Campos-Junior, Peter Mallmann, Ricardo Azambuja, Ricardo M. Marinho, Richard A. Anderson, Robert Jach, Roberto de A. Antunes, Rod Mitchell, Rouhollah Fathi, Satish Kumar Adiga, Seido Takae, Seok Hyun Kim, Sergio Romero, Silvana Chedid Grieco, Talya Shaulov, Tatsuro Furui, Teresa Almeida-Santos, Willianne Nelen, Yasmin Jayasinghe, Yodo Sugishita, Teresa K. Woodruff
JCO GLOBAL ONCOLOGY
(2020)
Article
Oncology
Alexandra S. Rashedi, Saskia F. de Roo, Lauren M. Ataman, Maxwell E. Edmonds, Adelino Amaral Silva, Anibal Scarella, Anna Horbaczewska, Antoinette Anazodo, Ayse Arvas, Bruno Ramalho de Carvalho, Cassio Sartorio, Catharina C. M. Beerendonk, Cesar Diaz-Garcia, Chang Suk Suh, Claudia Melo, Claus Yding Andersen, Eduardo Motta, Ellen M. Greenblatt, Ellen Van Moer, Elnaz Zand, Fernando M. Reis, Flor Sanchez, Guillermo Terrado, Jhenifer K. Rodrigues, Joao Marcos de Meneses e Silva, Johan Smitz, Jose Medrano, Jung Ryeol Lee, Katharina Winkler-Crepaz, Kristin Smith, Ligia Helena Ferreira Melo e Silva, Ludwig Wildt, Mahmoud Salama, Maria del Mar Andres, Maria T. Bourlon, Mario Vega, Mauricio Barbour Chehin, Michel De Vos, Mohamed Khrouf, Nao Suzuki, Osama Azmy, Paula Fontoura, Paulo Henrique Almeida Campos-Junior, Peter Mallmann, Ricardo Azambuja, Ricardo M. Marinho, Richard A. Anderson, Robert Jach, Roberto de A. Antunes, Rod Mitchell, Rouhollah Fathi, Satish Kumar Adiga, Seido Takae, Seok Hyun Kim, Sergio Romero, Silvana Chedid Grieco, Talya Shaulov, Tatsuro Furui, Teresa Almeida-Santos, Willianne Nelen, Yasmin Jayasinghe, Yodo Sugishita, Teresa K. Woodruff
JCO GLOBAL ONCOLOGY
(2020)