Article
Medicine, Legal
Natasha A. Karp, Louise Coleman, Peter Cotton, Nicola Powles-Glover, Amanda Wilson
Summary: Non-clinical rodent safety studies are crucial in assessing potential adverse effects of new medicines. AstraZeneca's implementation of repeated microsampling of main study animals shows that it has limited impact on blood variables and can reduce the number of animals used in toxicological assessments.
REGULATORY TOXICOLOGY AND PHARMACOLOGY
(2023)
Article
Pediatrics
Yarmarly Guerra Valero, Tavey Dorofaeff, Lisa Parker, Mark G. Coulthard, Louise Sparkes, Jeffrey Lipman, Steven C. Wallis, Jason A. Roberts, Suzanne L. Parker
Summary: The study found that both parents/guardians and bedside nurses prefer microsampling over conventional sampling for conducting pediatric pharmacokinetic clinical studies. Microsampling can reduce pain for children participating in these studies and support increased participation.
PEDIATRIC RESEARCH
(2022)
Article
Agriculture, Dairy & Animal Science
Maria Irene Pacini, Francesca Bonelli, George Lubas, Micaela Sgorbini
Summary: Given the increasing number of wild animal hospitalisations, it is important to improve veterinarians' knowledge of wildlife medicine and enhance clinical and diagnostic procedures. Blood tests are a quick and minimally invasive method to gather valuable clinical information for adequate treatment. This study evaluates the haematological and biochemical parameters in injured roe deer and suggests their relevance to animal hospitalisation outcomes.
Article
Veterinary Sciences
Witkowska-Pilaszewicz Olga, Cywinska Anna, Michlik-Polczynska Katarzyna, Czopowicz Michal, Strzelec Katarzyna, Biazik Anna, Parzeniecka-Jaworska Marta, Crisman Mark, Witkowski Lucjan
Summary: In ponies of different classes, all biochemical parameters, except total protein concentration, showed significant differences. The most significant difference was found in blood lactate concentrations, which were higher in the smallest ponies (class A). Muscle enzymes and urea were higher, while triglycerides and creatinine were lower than normal values for equine species in all groups of ponies. Blood lactate concentration was higher only in class A ponies compared to normal values for horses.
BMC VETERINARY RESEARCH
(2021)
Review
Fisheries
Huijie Chen, Daji Luo
Summary: This article summarizes the recent advancements in haematology health indicators in fish, analyzes the research progress and trends in haematology indicators in farmed fish, explores their potential application for healthy farming, and discusses the environmental factors that affect blood physiological and biochemical indices.
REVIEWS IN AQUACULTURE
(2023)
Article
Agriculture, Dairy & Animal Science
Sathita Areerat, Pipatpong Chundang, Chalermpol Lekcharoensuk, Attawit Kovitvadhi
Summary: This study explored the possibility of replacing poultry meal with insects in canine diets, finding that AD and BM can substitute poultry meal without adverse effects on health and nutrient digestibility in dogs.
Review
Medical Laboratory Technology
Nick Verougstraete, Veronique Stove, Christophe P. P. Stove
Summary: The collection of capillary blood microsamples via finger-prick is convenient and patient-centric, enabling remote monitoring of diabetes patients. However, there is heterogeneity in the study designs and data evaluation on HbA(1c) determination using microsampling. This review provides a critical overview of the literature and discusses the challenges and possibilities of implementing microsampling for reliable HbA(1c) determination, including the use of liquid blood microsamples.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Review
Toxicology
Kazuaki Takahashi, Norimichi Hattori, Hideaki Yokoyama, Fumihiro Jinno, Hirohiko Ohtsuka, Keiko Nakai, Asuka Takumi, Yusuke Shibui, Koki Yamaguchi, Taishi Shimazaki, Yoichi Tanaka, Kosuke Saito, Akio Kobayashi, Yoshiro Saito
Summary: Recently, animal welfare and the implementation of 3Rs in drug development have gained global attention. Microsampling, a method that collects small amounts of blood, is becoming popular in toxicokinetic assessment. This review summarizes the toxicological influence of microsampling in rodents, as well as the potential effects related to blood sampling site or volume. It also discusses the increased use of microsampling in regulatory toxicology studies and its benefits for animal welfare and the 3Rs.
JOURNAL OF APPLIED TOXICOLOGY
(2023)
Article
Chemistry, Analytical
Thomas Meikopoulos, Olga Begou, Georgios Theodoridis, Helen Gika
Summary: A new method for quantitative analysis of four ceramide species in dried blood samples was developed using LC-MS/MS. The Capitainer device was utilized to provide accurate sample volumes, allowing for accurate determinations. The study showed the potential application of at-home blood microsampling for health monitoring.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biochemistry & Molecular Biology
Camilla Marasca, Maria Encarnacion Blanco Arana, Michele Protti, Andrea Cavalli, Laura Mercolini, Andrea Armirotti
Summary: This study explores the potential of using solid supports and devices for blood microsampling and untargeted lipidomic profiling, with VAMS being identified as a viable option that offers advantages such as independence from hematocrit.
Article
Biochemical Research Methods
Suzanne L. Parker, Steven C. Wallis, Cheryl Fourie, Melissa Lassig-Smith, Therese Starr, Avinash Chikatamarla, Tavey Dorofaeff, Mark D. Chatfield, Jeffrey Lipman, Jason A. Roberts
Summary: Reducing the volume of blood sampled from neonatal or paediatric patients is crucial for research purposes, with skin prick and capillary microsamples producing comparable results to conventional arterial blood sampling in terms of meropenem concentrations. CMS samples have been found to be stable when stored in the capillary tube for certain durations.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Review
Medical Laboratory Technology
Prerna Dodeja, Spiros Giannoutsos, Steve Caritis, Raman Venkataramanan
Summary: Microsampling is a technique that uses a small volume of biological matrix for quantitative analysis. Dried blood spots, a commonly used microsampling method, has limitations such as sample inhomogeneity and environmental degradation. A novel microsampling device called Volumetric Absorptive Microsampling (VAMS) has shown promise in resolving these issues and improving sample collection precision.
THERAPEUTIC DRUG MONITORING
(2023)
Article
Medical Laboratory Technology
Peng Xu, Kui Fang, Xiling Chen, Yangruiqi Liu, Zheqing Dong, Ji Zhu, Keda Lu
Summary: This study demonstrates that platelet clumps can affect platelet indices, leukocyte count, and erythrocyte count. The Sysmex XN-10 instrument is sensitive to positive samples with low platelet counts but insensitive to those with high platelet counts.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2022)
Article
Chemistry, Multidisciplinary
Ying Wang, Sarah J. Crowell, Alison Joyce, Lisa Dyleski, Dean Messing, Jennifer Cargill, Zhiping You, Sarah Murphy, Meghan Gomes, Boris Gorovits
Summary: The study evaluated the suitability of whole blood microsampling procedures in non-human primate to support toxicokinetic assessments of biotherapeutics. Results showed comparable drug concentration profiles from all sampling procedures, with key PK parameters obtained from microsampling samples being comparable to those obtained from serum samples. This confirms that microsampling can be used as a substitute for conventional sampling to support PK/TK studies in non-clinical product developments.
PHARMACEUTICAL RESEARCH
(2021)
Article
Veterinary Sciences
Gwenael Boulbria, Charlotte Teixeira Costa, Valerie Normand, Veronique Bachy, Daphne Rochel, Mathieu Brissonnier, Pauline Berton, Franck Bouchet, Arnaud Lebret
Summary: This study provides updated haematological reference intervals for sows at end-gestation, showing significant impact of parity on haematological parameters and the relation between haemoglobin values and reproductive performance.
PORCINE HEALTH MANAGEMENT
(2021)
Article
Medicine, Legal
Fiona Sewell, Ian Ragan, Graham Horgan, David Andrew, Thomas Holmes, Irene Manou, Boris P. Mueller, Tim Rowan, Barbara G. Schmitt, Marco Corvaro
Summary: There are currently three test guidelines for acute oral toxicity studies, but the subjectivity of one guideline may be hindering its wider use. In order to address this, the NC3Rs and EPAA collaborated to analyze historical data and provide recommendations on the recognition of 'evident toxicity'.
REGULATORY TOXICOLOGY AND PHARMACOLOGY
(2024)
Article
Medicine, Legal
Michael DeVito, Bas Bokkers, Majorie B. M. van Duursen, Karin van Ede, Mark Feeley, Elsa Antunes Fernandes Gaspar, Laurie Haws, Sean Kennedy, Richard E. Peterson, Ron Hoogenboom, Keiko Nohara, Kim Petersen, Cynthia Rider, Martin Rose, Stephen Safe, Dieter Schrenk, Matthew W. Wheeler, Daniele S. Wikoff, Bin Zhao, Martin van den Berg
Summary: In October 2022, the World Health Organization reevaluated the toxic equivalency factors (TEFs) for chlorinated dioxin-like compounds in a panel convened in Lisbon. This effort utilized an updated database, Bayesian dose response modeling, and meta-analysis to derive Best-Estimate TEFs. Applying these new TEFs may result in lower total toxic equivalents for dioxin-like chemicals.
REGULATORY TOXICOLOGY AND PHARMACOLOGY
(2024)