Article
Cell & Tissue Engineering
Valentina Orticelli, Andrea Papait, Elsa Vertua, Patrizia Bonassi Signoroni, Pietro Romele, Lorena Di Pietro, Marta Magatti, Luciana Teofili, Antonietta Rosa Silini, Ornella Parolini
Summary: This study investigated the potential of human term placenta-derived amniotic membrane MSCs (hAMSC) to support the ex vivo expansion of HSC and progenitor cells. The results suggest that hAMSCs are a valuable alternative to BM-MSC for enhancing the proliferation and maintaining stemness of CB-HSC, and can improve the colony forming unit ability and long-term culture initiating cells ability, addressing the need for high HSC content in CB units available for transplantation.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Ximena Bonilla, Ana Milena Lara, Manuela Llano-Leon, David A. Lopez-Gonzalez, David G. Hernandez-Mejia, Rosa Helena Bustos, Bernardo Camacho-Rodriguez, Ana-Maria Perdomo-Arciniegas
Summary: Umbilical cord blood is an important source of hematopoietic stem and progenitor cells, and recent research has focused on developing ex vivo expansion strategies for these cells. Co-culturing the cells with specific mesenchymal stromal cells has been found to significantly increase their cellular dose and regulate their proliferation capacity. Additionally, the secretory profile of umbilical cord blood-derived cells closely resembles that of bone marrow-derived cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Rasha S. S. Teleb, Amal Abdul-Hafez, Amira Othman, Ahmed El-Abd Ahmed, Abdelrahman A. A. Elsaid, Hattan Arif, Ahmed A. A. Zarea, Mohammed Abdulmageed, Hend Mohamed, Sherif Abdelfattah Ibrahim, Ranga P. P. Thiruvenkataramani, Tarek Mohamed, Masamitsu Kanada, Burra V. V. Madhukar, Myrna Gonzalez Arellano, Mohammed M. M. Sayed, Heba M. M. Qubaisy, Said A. A. Omar
Summary: The objective of this study was to examine the effect of cord blood plasma (CBP)-derived exosomes (CBP Exos) and placental MSCs-derived exosomes (MSCs Exos) on the expansion of umbilical cord blood hematopoietic stem cells (UCB HSCs) and to maintain their primitive characteristics. The results showed that CBP- and placental-derived exosomes significantly enhanced the ex vivo expansion of UCB HSCs while preserving their primitive features.
Article
Biochemistry & Molecular Biology
Valentina Russo, Annunziata Mauro, Alessia Peserico, Oriana Di Giacinto, Mohammad El Khatib, Maria Rita Citeroni, Emanuela Rossi, Angelo Canciello, Eleonora Mazzotti, Barbara Barboni
Summary: This research demonstrates the potential of amniotic epithelial stem cells (AECs) in promoting tendon regeneration. The study found that AECs can trans-differentiate into cell phenotypes with varying abilities to facilitate regeneration. The findings suggest that AECs with an epithelial phenotype have a greater impact on shifting the inflammatory response to a pro-regenerative response, while AECs with a tendon-like phenotype mainly affect the proliferative phase of regeneration. These results provide support for using AECs as a feasible and effective strategy for the treatment of acute tendinopathies.
Review
Microbiology
Tony Cundell, J. Wade Atkins, Anna F. Lau
Summary: In the past two decades, the rapid advancement of technology has allowed for the widespread use of cell and gene therapy products in treating various diseases. This study reviewed literature from 2003 to 2021 to identify trends in microbial contamination of hematopoietic stem cells (HSCs) derived from different sources. The study also discussed regulatory context, testing expectations, clinical risks, and current practices for manufacturing and testing of HSCs.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Engineering, Biomedical
Zixuan Zhou, Jingnan Xun, Chenghao Wu, Chao Ji, Shizhao Ji, Futing Shu, Yuxiang Wang, Hao Chen, Yongjun Zheng, Shichu Xiao
Summary: This study demonstrates that micronized amniotic membrane (mAM) can be used as a microcarrier to improve the proliferation efficiency of umbilical cord-derived mesenchymal stem cells (UC-MSC) in vitro and accelerate burn wound healing when used in combination with UC-MSC (mAM-MSC). Transcriptome sequencing revealed upregulation of genes related to growth factors, angiogenesis, and wound healing in mAM-MSC compared to traditional 2D-cultured MSC, which was confirmed by RT-qPCR. Gene ontology analysis showed enrichment of terms related to cell proliferation, angiogenesis, cytokine activity, and wound healing in mAM-MSC. In a burn wound model, topical application of mAM-MSC promoted faster wound healing, increased MSC survival, and enhanced neovascularization.
MATERIALS TODAY BIO
(2023)
Article
Biochemistry & Molecular Biology
Sanshiro Kanazawa, Hiroyuki Okada, Dan Riu, Yo Mabuchi, Chihiro Akazawa, Junichi Iwata, Kazuto Hoshi, Atsuhiko Hikita
Summary: This study demonstrates the important role of c-Mpl signal in the proliferation and differentiation ability of mesenchymal stem cells (MSCs), which are maintained through co-culture with hematopoietic stem cells (HSCs). Furthermore, the study suggests the existence of a hematopoietic-mesenchymal signal and highlights the significance of HSC state in the stability of MSC properties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Santhosh Kacham, Tejal Sunil Bhure, Sindhuja D. Eswaramoorthy, Gaurav Naik, Subha Narayan Rath, Sreenivasa Rao Parcha, Sayan Basu, Virender Singh Sangwan, Sachin Shukla
Summary: Umbilical cord-derived mesenchymal stem cells promote the repair of injured corneal epithelium by stimulating the proliferation of corneal epithelial cells, potentially serving as a non-ocular source of stem cells for treating injury-induced bilateral corneal diseases.
Review
Cell Biology
Eleonora Russo, Giusi Alberti, Simona Corrao, Cesar V. Borlongan, Vitale Miceli, Pier Giulio Conaldi, Francesca Di Gaudio, Giampiero La Rocca
Summary: The potential of perinatal cells and their secreted EVs in regenerative medicine is well established. They exhibit therapeutic effects such as tissue repair, immune system modulation, and other functions. However, challenges remain in characterizing and utilizing these cells and EVs.
Article
Cell Biology
Li-Ping Liu, Dong-Xu Zheng, Zheng-Fang Xu, Hu-Cheng Zhou, Yun-Cong Wang, Hang Zhou, Jian-Yun Ge, Daisuke Sako, Mi Li, Kazunori Akimoto, Yu-Mei Li, Yun-Wen Zheng
Summary: This study reveals the commonalities and differences between amniotic epithelial stem cells (AESCs) and keratinocytes (KCs) through transcriptomics analysis and functional evaluation. The analysis shows that AESCs are involved in epidermis-associated biological processes shared with KCs, but they also exhibit distinct features. The overexpression of TP63 and activation of NOTCH signaling can enhance AESC stemness and improve their differentiation features.
Article
Cell & Tissue Engineering
Sara Bucar, Andre Dargen de Matos Branco, Marcia F. Mata, Joao Coutinho Milhano, Iris Caramalho, Joaquim M. S. Cabral, Ana Fernandes-Platzgummer, Claudia L. da Silva
Summary: The study compared the capacity of MSC from different sources to expand UCB CD34(+) cells ex vivo, with AT-derived MSC showing superior expansion potential and maintenance of primitive cells. UCM-derived MSC demonstrated inferior hematopoietic supportive capacity compared to MSC from adult tissues. Further research is needed to determine the impact of HPL on the hematopoietic supportive capacity of MSC.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell Biology
Chao-Ling Yao, Poyin Huang, Tsai-Chi Liu, Yung-Kai Lin, Ching-Yun Chen, Yi-Ting Lai, Tzu Yun Chin, Tsung-Yu Tseng, Yi-Chiung Hsu
Summary: Different experimental strategies for ex vivo expansion of human hematopoietic stem cells (HSCs) have been developed, however, the genomic function of expanded HSCs under different culture systems remains unclear. This study compared gene expression profiles of HSCs in serum and serum-free culture systems, identifying differentially expressed genes enriched in the TNF-regulated inflammatory pathway in the FBS culture system. The ex vivo expansion of HSCs using the FBS culture system induces an inflammatory response and high CD38 expression, providing new insights into genomic functions of HSCs under different expanded cultures.
Article
Biochemistry & Molecular Biology
Yushi Abe, Daigo Ochiai, Masako Taguchi, Seiji Kanzaki, Satoru Ikenoue, Yoshifumi Kasuga, Mamoru Tanaka
Summary: Human amniotic fluid stem cells (hAFSCs) can form aggregates with host immune cells and alleviate inflammation. Additionally, hAFSCs can increase regulatory T cells in the acquired immune system. Therefore, hAFSCs play an important role in the treatment of inflammation-related diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
L. B. Buravkova, M. Ezdakova, I. Andrianova, A. N. Gornostaeva, P. Bobyleva, E. R. Andreeva
Summary: The study demonstrated that during ex vivo expansion, cord blood hematopoietic stem progenitor cells (cbHSPCs) can influence the functional state of mesenchymal stromal cells (MSCs), leading to the formation of early stromal progenitors with a bipotential osteo-adipogenic profile. The joint coculture resulted in a specific hematopoiesis-supportive environment, with the expression of certain MSC genes upregulated and a balance of myeloid regulators maintained. Short-term osteoinduction prior to coculture enhanced the development of a bipotential profile, indicating a more committed state of cbHSPCs.
Article
Biochemistry & Molecular Biology
Naiyou Liu, Charles M. Bowen, Mohammadali M. Shoja, Karen Larissa Castro de Pereira, Laxmi Priya Dongur, Antonio Saad, William K. Russell, Thomas Christopher Broderick, Jeffrey H. Fair, William Samuel Fagg
Summary: Myofibroblast activation is a cellular response to physiological or pathological insults. In this study, co-cultured amniotic cell conditioned media (coACCM) was found to activate myofibroblast, while amniotic fluid (AF) represses it. These findings suggest that different biologics can elicit different cellular responses and may have broad utility in the clinic and the lab.
Article
Cell Biology
Adeleh Taghikhani, Zuhair Mohammad Hassan, Marzieh Ebrahimi, Seyyed-Mohammad Moazzeni
JOURNAL OF CELLULAR PHYSIOLOGY
(2019)
Review
Pharmacology & Pharmacy
Farzaneh Sharifzad, Saeid Ghavami, Javad Verdi, Soura Mardpour, Mahsa Mollapour Sisakht, Zahra Azizi, Adeleh Taghikhani, Marek J. Los, Esmail Fakharian, Marzieh Ebrahimi, Amir Ali Hamidieh
DRUG RESISTANCE UPDATES
(2019)
Article
Cell Biology
Mahsa Rahimi, Ali Sharifi-Zarchi, Javad Firouzi, Mahnaz Azimi, Nosratollah Zarghami, Effat Alizadeh, Marzieh Ebrahimi
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2019)
Article
Immunology
Ehsan Rezaie, Hekmat Nekoie, Ali Miri, Gholamreza Oulad, Ali Ahmadi, Mojtaba Saadati, Mahmood Bozorgmehr, Marzieh Ebrahimi, Jafar Salimian
MICROBIAL PATHOGENESIS
(2019)
Article
Cell Biology
Farzaneh Sharifzad, Hamed Yasavoli-Sharahi, Saeid Mardpour, Esmaeil Fakharian, Hassan Nikuinejad, Yasaman Heydari, Soura Mardpour, Adeleh Taghikhani, Reza Khellat, Somayeh Vafaei, Sahar Kiani, Saeid Ghavami, Marek Los, Mehdi Noureddini, Marzieh Ebrahimi, Javad Verdi, Amir Ali Hamidieh
JOURNAL OF CELLULAR PHYSIOLOGY
(2019)
Review
Oncology
Alaleh Rezalotfi, Elmira Ahmadian, Hossein Aazami, Ghasem Solgi, Marzieh Ebrahimi
FRONTIERS IN ONCOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Mahdieh Bakhshi, Jahanbakhsh Asadi, Marzieh Ebrahimi, Abdol-Vahab Moradi, Monireh Hajimoradi
JOURNAL OF CELLULAR BIOCHEMISTRY
(2019)
Article
Biochemistry & Molecular Biology
Reyhaneh Khoshchehreh, Mehdi Totonchi, Juan Carlos Ramirez, Raul Torres, Hossein Baharvand, Alexandra Aicher, Marzieh Ebrahimi, Christopher Heeschen
Review
Oncology
Somayeh Vafaei, Fahimeh Fattahi, Marzieh Ebrahimi, Leila Janani, Ahmad Shariftabrizi, Zahra Madjd
CANCER MANAGEMENT AND RESEARCH
(2019)
Review
Immunology
Hajar Rajayi, Parsova Tavasolian, Alaleh Rezalotfi, Marzieh Ebrahimi
INTERNATIONAL REVIEWS OF IMMUNOLOGY
(2019)
Article
Medicine, Research & Experimental
Samila Farokhimanesh, Mehdi Forouzandeh Moghadam, Marzieh Ebrahimi
IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES
(2020)
Article
Cell Biology
Mahsa Rahimi, Ali Sharifi-Zarchi, Nosratollah Zarghami, Lobat Geranpayeh, Marzieh Ebrahimi, Effat Alizadeh
Article
Pharmacology & Pharmacy
Marzieh Ebrahimi, Samira Barjasteh, Nasim Tolooyi Kashtiban
JOURNAL OF PHARMACEUTICAL RESEARCH INTERNATIONAL
(2019)
Article
Pharmacology & Pharmacy
Atefeh Safaipour, Marzieh Ebrahimi, Seyed Abolhassan Shahzadeh Fazeli, Mohammad Ali Amoozegar
IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH
(2019)
Article
Cell Biology
Niloufar Shayan Asl, Farhad Nejat, Parvaneh Mohammadi, Abdolhossein Nekoukar, Saeed Hesam, Marzieh Ebrahimi, Khosrow Jadidi
