Article
Allergy
Rafael Gras-Pena, Nichole M. Danzl, Mohsen Khosravi-Maharlooei, Sean R. Campbell, Amanda E. Ruiz, Christopher A. Parks, William Meng Suen Savage, Markus A. Holzl, Debanjana Chatterjee, Megan Sykes
Summary: This study developed a novel differentiation protocol to generate human thymic epithelial progenitors from human embryonic stem cells (hES-TEPs) and demonstrated their thymopoietic function in vivo. By incorporating hES-TEPs into a supportive thymic structure, the researchers enhanced human thymocyte development and increased the reconstitution of peripheral CD4+ naive T cells.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2022)
Article
Biology
Jung Ho Park, Yeon Ju Choi, So Young Kang, Hyunjeong Ju, Kyueng-Whan Min, Nan Young Kim, Ha Young Park, Eun Soo Kim, Mi Jung Kwon, Yong Joon Suh
Summary: This study investigated the viability and safety of intra-organ human adipose-derived stem cell (h-ADSC) xenotransplants in vivo. The results showed that h-ADSCs were able to survive after transplantation and did not cause inflammation. However, the survival rates varied depending on the organ.
Article
Biochemistry & Molecular Biology
Kuo-An Chu, Chang-Ching Yeh, Chun-Hsiang Hsu, Chien-Wei Hsu, Fu-Hsien Kuo, Pei-Jiun Tsai, Yu-Show Fu
Summary: This study compared the effectiveness of human umbilical cord Wharton's jelly-derived mesenchymal stem cells (HUMSCs) versus adipose tissue-derived mesenchymal stem cells (ADMSCs) for reversing pulmonary fibrosis in rats. The results showed that HUMSCs had better effects on lung function improvement and collagen degradation, as well as alveolar regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Weichao Zhai, Jerome Tan, Tobias Russell, Sixun Chen, Dennis McGonagle, May Win Naing, Derrick Yong, Elena Jones
Summary: The study investigates alternative methods for quantifying the senescence of hMSCs, aiming to increase the final output of therapeutic-grade hMSC cultures. The advanced flow cytometry and autofluorescence methods show statistically significant values and good fold difference ranges between early and senescent passage hMSCs, providing a label-free approach for evaluating cellular senescence in hMSCs.
SCIENTIFIC REPORTS
(2021)
Article
Biology
Vanessa Ho, Jonathan R. Baker, Keith R. Willison, Peter J. Barnes, Louise E. Donnelly, David R. Klug
Summary: An amplification-free sandwich hybridisation assay using a microfluidic platform is developed to detect single miRNA molecules in single cells. The absolute quantification of miRNAs at a single cell level is achieved and validated using real-time qPCR. The sensitivity of the assay is demonstrated by quantifying single miRNA molecules in nasal fluid, showing its potential utility in clinical studies.
COMMUNICATIONS BIOLOGY
(2023)
Review
Medicine, General & Internal
Reem Saud Alhejailan, Gloria Garoffolo, Vineesh Vimala Raveendran, Maurizio Pesce
Summary: After more than 20 years of research in regenerative medicine, the best cell types and materials to effectively translate into clinical practice for cardiac diseases are still uncertain. It is now clear that the heart lacks a consistent reservoir of stem cells for generating new myocytes, and other cells may only contribute with their pro-angiogenic or immunomodulatory potential. New developments in somatic cells' reprogramming, material science, and cell biophysics may be helpful in protecting the heart and enhancing its lost regenerative potential.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Cell Biology
Hitesh Chopra, Chen Cao, Celia Sommer, Alex Dahlkemper, James Sugai, James L. Sherley, Darnell Kaigler
Summary: This study quantifies the stem cell fractions of human mesenchymal stem cell preparations derived from oral tissues and finds significant differences in the stem cell fractions among donors. The stability of the stem cell fractions during serial cell culture shows inter-donor variation, with some patient preparations exhibiting sufficient stability to support long-term expansion of stem cells. These findings provide important insights for the clinical-scale expansion and biomanufacturing of MSCs, leading to more effective and predictable outcomes in clinical trials and treatments employing SCT.
Review
Cell Biology
Yiyi Xuan, Bjorn Petersen, Pentao Liu
Summary: This review comprehensively discusses the potential applications of human and pig pluripotent stem cells (PSCs) and provides an overview of the current progress and challenges. It explores the therapeutic uses of PSC-derived cellular products and emphasizes the importance of pig PSCs in genetic engineering for pig-to-human xenotransplantation. Further investigations and refinement of PSC technologies are necessary to unlock their full potential in regenerative medicine.
Review
Cell Biology
Juan Huang, Qi Feng, Li Wang, Bingying Zhou
Summary: Cardiac diseases are the leading cause of global mortality, but limited progress has been made in developing therapeutic options. The use of animal models has limitations due to species-specific differences, leading to inaccurate drug testing results. Human pluripotent stem cell (hPSC) technology offers promising alternatives for heart disease modeling, cell therapy, and drug discovery, with potential for revolutionizing these fields with further optimization required.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Swati Mishra, Jasin Taelman, Yolanda W. Chang, Annekatrien Boel, Petra De Sutter, Bjorn Heindryckx, Susana M. Chuva De Sousa Lopes
Summary: The study found that a combination of TNAP and PDPN was effective in isolating populations of early FGCs and hPGCLCs in both sexes, with female hPGCLCs retaining stronger colony-forming ability in culture medium.
Article
Oncology
Ledio Bocova, Wouter Hubens, Cordula Engel, Steffen Koschmieder, Edgar Jost, Wolfgang Wagner
Summary: In this study, researchers demonstrate that hematopoietic stem and progenitor cells (HSPCs) can be estimated by targeted DNA methylation (DNAm) analysis. DNAm levels at specific CpG sites in the genes MYO1D, STK17A, and SP140 were found to correlate with CD34(+) cell numbers in mobilized peripheral blood and blast counts in leukemia. These epigenetic biomarkers could potentially be used to assess stem cell mobilization, HSPC harvesting, or blast count in leukemia.
CLINICAL EPIGENETICS
(2023)
Article
Immunology
Fen Zhang, Linxiao Fan, Qiuhong Liu, Shima Tang, Sainan Zhang, Lanlan Xiao, Lingjian Zhang, Qian Li, Nueraili Maihemuti, Lanjuan Li
Summary: MenSCs treatment can alleviate autoimmune hepatitis by inhibiting macrophages and M1 polarization in the liver tissue.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Kevin McKinski, Dean McNulty, Francesca Zappacosta, Mary Birchler, Matt Szapacs, Christopher Evans
Summary: ICOS, expressed on the surface of activated T-cells, is being investigated as a potential therapeutic target. Recent studies found aberrant increases in sICOS in disease-state patients using commercial ELISA kits, but the results were not consistent with in-house immunoassays. The researchers developed a novel LC-MS/MS method to confirm the results and demonstrated that sICOS is a low abundance soluble protein.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2022)
Article
Biochemical Research Methods
Sara Khaleghi, Farideh Eivazkhani, Somayeh Tavana, Ashraf Moini, Marefat Ghaffari Novin, Petkov Stoyan, Hamid Nazarian, Rouhollah Fathi
Summary: The study aimed to construct an Artificial Ovary by seeding Human Ovarian Cortical Cells into Human ovarian Decellularized Cortical Tissue, and assessed its ability to produce new ovarian follicles and hormone secretion after xenotransplantation.
JOURNAL OF BIOLOGICAL ENGINEERING
(2023)
Article
Immunology
Rihan Wu, Ru Ma, Xiaojun Duan, Jiandong Zhang, Kexin Li, Lei Yu, Mingyang Zhang, Pengxia Liu, Changshan Wang
Summary: This study aimed to develop a comprehensive LUSC-specific DEGs signature and identified a seven-gene signature for prognosis prediction of LUSC patients. These genes were found to be associated with tumor progression, immune infiltration, and stem index. Experimental models confirmed that one of the genes, FGG, was significantly upregulated in tumor cells and correlated with immune tumor progression, immune infiltration, and stem cell maintenance.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Oncology
Camille Verocq, Christine Decaestecker, Laureen Rocq, Sarah De Clercq, Audrey Verrellen, Zita Mekinda, Sebahat Ocak, Christophe Compere, Claudia Stanciu-Pop, Isabelle Salmon, Myriam Remmelink, Nicky D'Haene
Article
Oncology
Louis Godin, Cedric Balsat, Yves-Remi Van Eycke, Justine Allard, Claire Royer, Myriam Remmelink, Ievgenia Pastushenko, Nicky D'Haene, Cedric Blanpain, Isabelle Salmon, Sandrine Rorive, Christine Decaestecker
Article
Neurosciences
Laetitia Lebrun, Barbara Melendez, Oriane Blanchard, Nancy De Neve, Claude Van Campenhout, Julie Lelotte, Danielle Baleriaux, Matteo Riva, Jacques Brotchi, Michael Bruneau, Olivier De Witte, Christine Decaestecker, Nicky D'Haene, Isabelle Salmon
ACTA NEUROPATHOLOGICA COMMUNICATIONS
(2020)
Article
Neurosciences
Aude Chiot, Sakina Zaidi, Charlene Iltis, Matthieu Ribon, Felix Berriat, Lorenzo Schiaffino, Ariane Jolly, Pierre de la Grange, Michel Mallat, Delphine Bohl, Stephanie Millecamps, Danielle Seilhean, Christian S. Lobsiger, Severine Boillee
NATURE NEUROSCIENCE
(2020)
Article
Geriatrics & Gerontology
Matthieu Ribon, Celine Leone, Aude Chiot, Felix Berriat, Martine Rampanana, Julie Cottin, Delphine Bohl, Stephanie Millecamps, Christian S. Lobsiger, Michael T. Heneka, Severine Boillee
Summary: Neuroinflammation is a hallmark of ALS, where deletion of S100a9 did not improve mouse survival but instead accelerated symptoms, indicating that blocking 5100-A9 is not a viable strategy for treating ALS.
NEUROBIOLOGY OF AGING
(2021)
Article
Oncology
Corentin Martens, Olivier Debeir, Christine Decaestecker, Thierry Metens, Laetitia Lebrun, Gil Leurquin-Sterk, Nicola Trotta, Serge Goldman, Gaetan Van Simaeys
Summary: Recent research on dynamic amino acid positron emission tomography (PET) in gliomas has shown potential for extracting key features with the use of principal component analysis (PCA) to better understand intra-tumor heterogeneity. The PCA model outperforms traditional pharmacokinetic modeling in distinguishing characteristic dynamic uptake behaviors within the tumor.
Article
Pathology
Claude Van Campenhout, Ricardo De Mendonca, Barbara Alexiou, Sarah De Clercq, Marie-Lucie Racu, Claire Royer-Chardon, Stefan Rusu, Marie Van Eycken, Maria Artesi, Keith Durkin, Patrick Mardulyn, Vincent Bours, Christine Decaestecker, Myriam Remmelink, Isabell Salmon, Nicky D'Haene
Summary: This study validates the feasibility of SARS-CoV-2 genome sequencing on FFPE lung tissues from deceased COVID-19 patients.
JOURNAL OF MOLECULAR DIAGNOSTICS
(2021)
Review
Neurosciences
Elise Liu, Lea Karpf, Delphine Bohl
Summary: Inflammation is a shared hallmark between ALS and FTD, now recognized as a potential driver of the diseases. The iPSC technology offers hope in studying intercellular interactions and disease-specific defects. New cellular tools could potentially lead to new therapeutic approaches for ALS, ALS-FTD, and FTD patients.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2021)
Editorial Material
Computer Science, Interdisciplinary Applications
Adrien Foucart, Olivier Debeir, Christine Decaestecker
Summary: The MoNuSAC 2020 challenge hosted at the ISBI 2020 conference has been analyzed, revealing three problems in the computation of the metric used for ranking. The incorrect code version was used to rank the algorithms in the challenge. The results can be replicated using the code provided on GitHub.
IEEE TRANSACTIONS ON MEDICAL IMAGING
(2022)
Article
Oncology
Corentin Martens, Antonin Rovai, Daniele Bonatto, Thierry Metens, Olivier Debeir, Christine Decaestecker, Serge Goldman, Gaetan Van Simaeys
Summary: This research proposed a deep learning-based approach to address problems in glioma growth models. By training deep neural networks, it is possible to reconstruct the cell-density distribution of the tumor and accurately evaluate model parameters. This approach has significant potential application value for glioma characterization and therapy planning.
Article
Computer Science, Artificial Intelligence
Adrien Foucart, Olivier Debeir, Christine Decaestecker
Summary: Biomedical image analysis competitions often use a single metric to rank participants, which makes it difficult to assess the strengths and weaknesses of algorithms. The MoNuSAC 2020 challenge provides an interesting opportunity to study the information lost by using entangled metrics by involving multiple capabilities and releasing prediction masks from different teams. The results analysis using Panoptic Quality (PQ) and disentangled metrics shows that PQ hides interesting aspects of the results and is sensitive to small changes in prediction masks, making it hard to interpret and draw insights from the results. Access to raw predictions from participating teams is necessary for better analysis and usefulness to the research community.
PATTERN RECOGNITION
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Corentin Martens, Laetitia Lebrun, Christine Decaestecker, Thomas Vandamme, Yves-Remi Van Eycke, Antonin Rovai, Thierry Metens, Olivier Debeir, Serge Goldman, Isabelle Salmon, Gaetan Van Simaeys
Summary: This study uses a 3D-printed slicer for stereotactic histological analysis of an unoperated brain with glioblastoma, revealing limitations of conventional MRI in deriving glioma cell density maps and emphasizing the need for other initialization methods for reaction-diffusion models in clinical practice.
Article
Clinical Neurology
Laetitia Lebrun, Martin Bizet, Barbara Melendez, Barbara Alexiou, Lara Absil, Claude Van Campenhout, Nicky D'Haene, Sandrine Rorive, Francois Fuks, Christine Decaestecker, Isabelle Salmon
Summary: In this study, 16 intramedullary astrocytoma (IMA) samples were analyzed, revealing that the current DNA methylation-based classification method is insufficient for accurate classification of IMAs. Further research is needed to provide pathologists with a more comprehensive tool for classification of IMAs.
JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY
(2021)
Review
Medicine, General & Internal
Yves-Remi Van Eycke, Adrien Foucart, Christine Decaestecker
FRONTIERS IN MEDICINE
(2019)
Proceedings Paper
Engineering, Biomedical
Adrien Foucart, Olivier Debeir, Christine Decaestecker
2019 IEEE 16TH INTERNATIONAL SYMPOSIUM ON BIOMEDICAL IMAGING (ISBI 2019)
(2019)
