Article
Chemistry, Multidisciplinary
Ouyang Dan, Chuanzhe Wang, Chao Zhong, Juan Lin, Gang Xu, Guane Wang, Zian Lin
Summary: In this study, novel LDI-MS substrates were designed to enhance the sensitivity of mass signals and achieve high accuracy diagnosis of different saccharides and central precocious puberty (CPP).
Article
Microbiology
Anil K. Persad, Hanan A. Fahmy, Nicholas Anderson, Jing Cui, Zeynal Topalcengiz, Saharuetai Jeamsripong, Patrick M. Spanninger, Robert L. Buchanan, Kalmia E. Kniel, Michele T. Jay-Russell, Michelle D. Danyluk, Gireesh Rajashekara, Jeffrey T. LeJeune
Summary: This study demonstrates that MALDI-TOF is a viable alternative for the rapid identification and differentiation of Salmonella serovars.
Review
Biochemical Research Methods
Lanaia Itala Louzeiro Maciel, Ricardo Alves Bernardo, Rafael Oliveira Martins, Almir Custodio Batista Jr, Joao Victor Ataide Oliveira, Andrea Rodrigues Chaves, Boniek Gontijo Vaz
Summary: The imaging of biological tissues using mass spectrometry imaging (MSI) techniques allows for the visualization of the distribution of various metabolites, drugs, lipids, and glycans in complex samples. MSI methods like DESI-MSI and MALDI-MSI provide high sensitivity and the ability to evaluate/visualize multiple analytes in a single sample, overcoming limitations of classical microscopy techniques. This review discusses the application of DESI-MSI and MALDI-MSI in evaluating exogenous and endogenous molecules in biological samples, providing technical insights and recent research findings.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Hua Zhang, Lin Zhao, Jingjing Jiang, Jie Zheng, Li Yang, Yanyan Li, Jian Zhou, Tianshu Liu, Jianmin Xu, Wenhui Lou, Weige Yang, Lijie Tan, Weiren Liu, Yiyi Yu, Meiling Ji, Yaolin Xu, Yan Lu, Xiaomu Li, Zhen Liu, Rong Tian, Cheng Hu, Shumang Zhang, Qinsheng Hu, Yangdong Deng, Hao Ying, Sheng Zhong, Xingdong Zhang, Yunbing Wang, Hua Wang, Jingwei Bai, Xiaoying Li, Xiangfeng Duan
Summary: As cancer is increasingly seen as a metabolic disorder, serum metabolite profiling is proposed as a viable method for detecting cancer. In this study, a machine learning model using mass spectrometry-based liquid biopsy data is reported for pan-cancer screening and classification. The Multiplexed Nanomaterial-Assisted LDI for Cancer Identification (MNALCI) achieves high sensitivity and specificity for distinguishing cancers from healthy controls and accurately identifying the tissue of origin of tumors. This high throughput assay shows promising potential for non-invasive cancer diagnosis.
NATURE COMMUNICATIONS
(2022)
Article
Medicine, General & Internal
Noboru Horinouchi, Seiji Shiota, Takeshi Takakura, Atsushi Yoshida, Ken Kikuchi, Akira Nishizono, Eishi Miyazaki
Summary: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry can reduce the misidentification of bacteria as C. sakazakii and improve the reporting rate of E. asburiae.
JOURNAL OF MEDICAL CASE REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
R. Gathercole, E. Tranfield, D. Xia, G. Perez-Cordon, G. Robinson, D. Timofte, F. Zendri, R. M. Chalmers
Summary: The study developed a method to purify Cryptosporidium spp. oocysts from clinical stool samples and evaluated them using mass spectrometry. The method produced distinct spectra specific to different Cryptosporidium species, demonstrating the utility of the purification method.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Chadaporn Chaiden, Janthima Jaresitthikunchai, Anusak Kerdsin, Nattakan Meekhanon, Sittiruk Roytrakul, Suphachai Nuanualsuwan
Summary: In this study, MALDI-TOF-MS analysis of extracted peptides was developed to improve the classification of S. suis serotypes. The method successfully differentiated highly pathogenic SS2 and SS14 from other serotypes, indicating its potential as an alternative approach for serotyping clinically pathogenic S. suis isolates.
Review
Microbiology
Xin-Fei Chen, Xin Hou, Meng Xiao, Li Zhang, Jing-Wei Cheng, Meng-Lan Zhou, Jing-Jing Huang, Jing-Jia Zhang, Ying-Chun Xu, Po-Ren Hsueh
Summary: MALDI-TOF MS has been used in clinical microbiology since 2010, offering advantages such as convenience, speed, accuracy, and low cost. It has shown promising results in rapid detection of highly virulent and drug-resistant pathogens, making it a valuable tool in clinical microbial identification.
Article
Infectious Diseases
Jichan Shi, Gexin Gao, Jing Pan, Lian-Peng Wu, Hongye Ning, Zhengxin Wu, Xinchun Ye, Xiangao Jiang
Summary: This study evaluated the clinical value of MALDI-TOF MS in detecting NTM by comparing the results with liquid culture and gene chip. The findings showed that MALDI-TOF MS had a high consistency with both methods and could be a rapid and effective method for identifying NTM strains and drug resistance.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Parasitology
Jurena Christiane Ebersbach, Marcello Otake Sato, Matheus Pereira de Araujo, Megumi Sato, Soeren L. Becker, Issa Sy
Summary: Schistosomiasis is a major tropical disease affecting millions of people worldwide. The current diagnostic methods have limitations, and this study developed and validated a species-specific database for adult Schistosoma identification. The study also evaluated the effects of different storage solutions on spectra profiles. The results showed that MALDI-TOF MS can reliably distinguish between S. mansoni and S. japonicum.
PARASITES & VECTORS
(2023)
Article
Immunology
John A. Fissel, Carrie K. Holdren-Serrell, Warda Memon, Sean X. Zhang
Summary: One of the challenges in fungal infection treatment is the long time required for species identification. MALDI-TOF MS has shown potential in rapidly identifying bacteria and yeasts, and this study explores its use for identifying filamentous molds. Most isolates in the study could be identified by MALDI-TOF MS within three days.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Plant Sciences
Ye Rin Jin, Myung Jin Oh, Heung Joo Yuk, Hyun Joo An, Dong Seon Kim
Summary: This study established a new mass spectrometry-based analysis procedure for red ginseng polysaccharides (RGPs), successfully qualitatively analyzing neutral and acidic RGPs, and identifying novel components. The new procedure shows great potential as a specific tool for searching and determining various polysaccharides in herbal medicines.
JOURNAL OF GINSENG RESEARCH
(2021)
Article
Plant Sciences
Yukun Liu, Xiaofei Nie, Jilong Wang, Zhenqi Zhao, Zhimei Wang, Fang Ju
Summary: Flavonoids in litchi seeds have broad-spectrum antiviral and antitumor activities, especially against breast and liver cancers. MALDI-MSI was used to detect and image the distribution of flavonoids in litchi seed tissue sections for the first time, revealing their heterogeneous distribution and potential for extraction.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Chi-Sheng Chen, Wen-Chi Chen, Sarah Y. Chang
Summary: A new mode of electromembrane extraction (EME) has been developed for detection via matrix-assisted laser desorption/ionization mass spectrometry (MALDI/MS). The method successfully extracted and analyzed posaconazole, and was applied to human serum samples.
Article
Food Science & Technology
Qi Wang, Xiaohui Li, Hongping Wang, Simeng Li, Chen Zhang, Xueying Chen, Jing Dong, Hua Shao, Jing Wang, Fen Jin
Summary: A matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) method was developed to obtain dynamic images of forchlorfenuron in oriental melons. The majority of forchlorfenuron was found in the exocarp and mesocarp regions of the melons and decreased within two days after application. The degradation rate obtained by MALDI-MSI was reliable and comparable to HPLC-MS/MS, indicating its practicability for pesticide degradation study.
Article
Biotechnology & Applied Microbiology
Jiri Vlach, Barbora Javurkova, Ludmila Karamonova, Martina Blazkova, Ladislav Fukal
Article
Chemistry, Applied
Sandra Sykorova, Lucie Fojtikova, Martin Kuchar, Petra Miksatkova, Ludmila Karamonova, Ladislav Fukal, Oldrich Lapcik, Barbora Holubova
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2018)
Article
Biochemical Research Methods
Pavel Cejnar, Stepanka Kuckova, Ales Prochazka, Ludmila Karamonova, Barbora Svobodova
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2018)
Article
Food Science & Technology
Barbora Holubova, Petra Miksatkova, Martin Kuchar, Ludmila Karamonova, Oldrich Lapcik, Ladislav Fukal
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Jiri Novotny, Barbora Svobodova, Jiri Santrucek, Ladislav Fukal, Ludmila Karamonova
Summary: This study presents an improved method for mapping the proteome of Cronobacter sakazakii, a genus responsible for severe infections. By fractionating the bacterial cells and using a novel protein identification approach, they identified a large number of proteins, providing important insights into the pathogenesis and virulence factors of this bacterium.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Review
Chemistry, Multidisciplinary
Tereza HuliCkova, Ludmila Karamonova
Summary: Circulating tumor cells are released from primary tumors into the blood or lymph, where they can potentially form metastases in other tissues. Their non-invasive collection, early detection, molecular characterization, and continuous monitoring of therapeutic effectiveness make them highly valuable in clinical practice. This paper discusses the mechanisms of tumor cell circulation, survival in the bloodstream, settlement in new organ microenvironments, as well as methods of separation and detection.
Article
Chemistry, Multidisciplinary
Jiri Novotny, Barbora Svobodova, Anna Jelinkova, Ludmila Karamonova
Summary: Cronobacter spp. is an opportunistic pathogen with various virulence factors that enable successful adhesion, invasion, survival, and evasion of host immune response, causing severe infections.
Article
Microbiology
Vera Bunesova, Jiri Killer, Barbora Javurkova, Eva Vlkova, Vaclav Tejnecky, Sarka Musilova, Vojtech Rada
Review
Chemistry, Multidisciplinary
Ludmila Karamonova, Barbora Holubova, Anna Jelinkova, Jiri Novotny, Barbora Svobodova
Article
Toxicology
Lucie Fojtikova, Anna Sulakova, Martina Blazkova, Barbora Holubova, Martin Kuchar, Petra Miksatkova, Oldrich Lapcik, Ladislav Fukal
TOXICOLOGY REPORTS
(2018)