Journal
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
Volume 25, Issue 7, Pages 969-972Publisher
WILEY
DOI: 10.1002/rcm.4939
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Funding
- BBSRC
- EPSRC
- Scottish Funding Council
- University of Glasgow
- University of Edinburgh
- Bill and Melinda Gates Foundation, Global Health [OPPPGH5337]
- Biotechnology and Biological Sciences Research Council [BB/C511572/1] Funding Source: researchfish
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Mass spectrometry imaging (MSI) is a powerful tool in metabolomics and proteomics for the spatial localization and identification of pharmaceuticals, metabolites, lipids, peptides and proteins in biological tissues. However, sample preparation remains a crucial variable in obtaining the most accurate distributions. Common washing steps used to remove salts, and solvent-based matrix application, allow analyte spreading to occur. Solvent-free matrix applications can reduce this risk, but increase the possibility of ionisation bias due to matrix adhesion to tissue sections. We report here the use of matrix-free MSI using laser desorption ionisation performed on a 12 T Fourier transform ion cyclotron resonance (FTICR) mass spectrometer. We used unprocessed tissue with no post-processing following thaw-mounting on matrix-assisted laser desorption ionisation (MALDI) indium-tin oxide (ITO) target plates. The identification and distribution of a range of phospholipids in mouse brain and kidney sections are presented and compared with previously published MALDI time-of-flight (TOF) MSI distributions. Copyright (C) 2011 John Wiley & Sons, Ltd.
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