Article
Chemistry, Analytical
Pei Su, Xi Chen, Andrew J. Smith, Michael F. Espenship, Hugo Y. Samayoa Oviedo, Solita M. Wilson, Habib Gholipour-Ranjbar, Carlos Larriba-Andaluz, Julia Laskin
Summary: In this study, an efficient approach to multiplex electrospray ionization sources for applications in analytical and preparative mass spectrometry is reported. By implementing up to four orthogonal injection inlets on the opposite sides of an electrodynamic ion funnel interface, the total ion current transmitted through the mass spectrometer and the signal-to-noise ratio increase significantly. The results demonstrate the potential for proportional increase in ion current with the number of ESI inlets, presenting exciting opportunities for the development of bright ion sources in mass spectrometry applications.
ANALYTICAL CHEMISTRY
(2021)
Article
Automation & Control Systems
Adam J. Taylor, Dimitrios Tsikritsis, Alex Dexter, Amy Burton, Josephine Bunch, Natalie A. Belsey
Summary: Rapid chemical characterization methods can assess and classify differences in solid oral dosage forms, helping to identify off-brand or counterfeit products.
JOURNAL OF CHEMOMETRICS
(2022)
Article
Food Science & Technology
Yunhe Hong, Nicholas Birse, Brian Quinn, Holly Montgomery, Di Wu, Goncalo Rosas da Silva, Saskia M. van Ruth, Christopher T. Elliott
Summary: This study used DESI-MS to analyze and classify biomarkers in milk from five different animal and plant sources for the first time. Differences in spectra were observed between cow milk, goat milk, camel milk, soya milk, and oat milk. The classification of different milk sources achieved a 100% cross-validation rate through LDA, with high sensitivity to adulteration. DESI-MS was shown to be a rapid and efficient approach for milk fraud control.
NPJ SCIENCE OF FOOD
(2022)
Article
Chemistry, Analytical
Bertrand Rochat, Patrice Waridel, Jachen Barblan, Pierre-Edouard Sottas, Manfredo Quadroni
Summary: The study presents a new workflow for LC-MS determination of native peptides in plasma at picomolar levels. Evaluation of extract reproducibility, robustness, extraction yields, matrix effects, calibration curves and limits of detection were carried out. The method shows promise for peptide determination in various biomatrices with low picomolar limits of detection.
Article
Biochemistry & Molecular Biology
Catia Costa, Janella De Jesus, Chelsea Nikula, Teresa Murta, Geoffrey W. W. Grime, Vladimir Palitsin, Veronique Dartois, Kaya Firat, Roger Webb, Josephine Bunch, Melanie J. J. Bailey
Summary: The co-localization of elemental species with host biomolecules allows for the understanding of metabolic dysregulation and disease pathogenesis. Alkali metals, widely studied and implicated in various diseases, disrupt lipid metabolism. Previous work in DESI molecular imaging has shown that the commonly used solvent system leads to ion delocalization, hindering subsequent elemental analysis. A novel solvent system (50:50 (%v/v) MeOH:EtOH) is presented here, which preserves the elemental species and enables post-DESI elemental mapping on the same tissue section. Benchmarking the MeOH:EtOH solvent against the commonly used MeOH:H2O solvent revealed improved lipid signal-to-noise ratios. The multimodal imaging workflow was successfully applied to a lung tissue section containing a tuberculosis granuloma, demonstrating its applicability to structurally defined, elementally-rich samples.
Article
Chemistry, Analytical
Nicole M. Brundridge, Jonathan Dickerhoff, Danzhou Yang, Scott A. Mcluckey
Summary: This study investigates the preservation and stability of G-quadruplex (G4) DNA structure in gas phase using mass spectrometry. The results show that G4 structure exhibits distinct fragmentation behavior compared to non-G4 structure under low energy activation, indicating its stability in the gas phase.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Lana Abou-Zeid, Albert Pell, Marta Garcia-Cortes, Helene Isnard, Pascale Delangle, Carole Bresson
Summary: A cyclic tetra-phosphorylated biomimetic peptide (pS1368) was investigated as a potential starting structure for designing a decorporating agent of uranyl (UO22+). The peptide's selectivity towards UO22+ in the presence of competing endogenous elements (Ca2+, Cu2+, Zn2+) was determined using HILIC-ESI-MS/ICP-MS simultaneous coupling. The study demonstrated that pS1368 showed selectivity towards UO22+ in the presence of Sr2+, Cu2+, and Zn2+.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Monika Beszterda, Rafal Franski
Summary: This study aimed to analyze the ESI-MS behavior of flavonoid 5-O-glucosides in cherry tree bark, compare it with their positional isomers, and utilize the susceptibility to hydrolysis for identification. The results showed that under acid conditions, flavonoid 5-O-glycosides were completely hydrolyzed, giving distinctive ions in positive and negative ion modes for effective differentiation from their positional isomers.
PHYTOCHEMICAL ANALYSIS
(2021)
Article
Multidisciplinary Sciences
Steve Sweet, David Chain, Wen Yu, Philip Martin, Marlon Rebelatto, Andrew Chambers, Fabiola Cecchi, Yeoun Jin Kim
Summary: Mass spectrometry-based targeted proteomics allows objective protein quantitation of clinical biomarkers. The use of high-field asymmetric waveform ion mobility spectrometry and parallel reaction monitoring improves assay sensitivity and enables quantitation of proteins in clinical samples.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Analytical
Renata Konasova, Dusan Koval, Jan Hosek, Vaclav Kasicka
Summary: In CE-ESI-MS interfaces, protruding or aligning the capillary tip with the emitter tip affects the decoupling of the ESI potential from the separation potential. Only when the capillary tip is retracted 20 μm inside the emitter can complete decoupling of the spray and separation potentials be achieved, providing better sensitivity for the analysis.
Article
Chemistry, Analytical
Sergio Cancho-Gonzalez, Paul Ferguson, Julie M. Herniman, G. John Langley
Summary: Poly(ethylene glycols) are complex polymers commonly added to pharmaceutical formulations for improved drug solubility and delivery. This study employs a supercritical fluid chromatography-mass spectrometry approach to selectively ionize poly(ethylene glycols) as ammoniated molecules, simplifying data analysis and enabling batch-to-batch comparisons.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Ioana Cezara Caba, Raluca Stefanescu, Bogdan Ionel Tamba
Summary: This study used mass spectrometry to investigate the formation of noncovalent complexes between oleuropein and amyloid beta. By modifying the chemical environment and conducting proteolytic experiments, the binding site was identified. The results highlight the importance of further research on the effect of oleuropein against amyloid beta aggregation.
Article
Multidisciplinary Sciences
Shane C. Wright, Aikaterini Motso, Stefania Koutsilieri, Christian M. Beusch, Pierre Sabatier, Alessandro Berghella, Elodie Blondel-Tepaz, Kimberley Mangenot, Ioannis Pittarokoilis, Despoina-Christina Sismanoglou, Christian Le Gouill, Jesper V. Olsen, Roman A. Zubarev, Nevin A. Lambert, Alexander S. Hauser, Michel Bouvier, Volker M. Lauschke
Summary: This study investigates the subcellular location of GLP-1R signaling events and reveals associations between signaling profiles and adverse drug reactions, providing important insights for rational drug design and improving the therapeutic potential of GLP-1R agonists.
NATURE COMMUNICATIONS
(2023)
Article
Veterinary Sciences
Zehra Hajrulai-Musliu, Risto Uzunov, Stefan Jovanov, Maksud Kerluku, Dean Jankuloski, Velimir Stojkovski, Lazo Pendovski, James Jacob Sasanya
Summary: A sensitive analytical method for the determination of 69 analytes in bovine milk using liquid chromatography electrospray ionisation-tandem mass spectrometry was developed. The method successfully detected residues of antimicrobial, anabolic hormone, lactone, beta-agonist, mycotoxin, and pesticide residues in raw milk samples.
JOURNAL OF VETERINARY RESEARCH
(2022)
Article
Physics, Atomic, Molecular & Chemical
Pandi Boomathi Pandeswari, Varatharajan Sabareesh
Summary: The study shows that arginine has a greater influence on the CID MS/MS behavior of peptides with lengths greater than 25 amino acid residues compared to smaller peptides. Chemical modifications and mutations of arginine residues in peptides result in different stoichiometric products, with mutated peptides showing higher sequence coverage in CID MS/MS spectra.
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
(2021)
Article
Energy & Fuels
John J. Milledge, Birthe V. Nielsen, Manar S. Sadek, Patricia J. Harvey
Article
Biotechnology & Applied Microbiology
John J. Milledge, Birthe V. Nielsen, Patricia J. Harvey
JOURNAL OF APPLIED PHYCOLOGY
(2019)
Article
Biochemical Research Methods
Uttam K. Roy, Nathalie Lavignac, Azizur M. Rahman, Birthe V. Nielsen
JOURNAL OF CHROMATOGRAPHIC SCIENCE
(2018)
Article
Nutrition & Dietetics
Fernando Naclerio, Eneko Larumbe-Zabala, Marcos Seijo, Nadia Ashrafi, Birthe V. Nielsen, Conrad P. Earnest
JOURNAL OF THE AMERICAN COLLEGE OF NUTRITION
(2019)
Review
Energy & Fuels
John J. Milledge, Birthe V. Nielsen, Supattra Maneein, Patricia J. Harvey
Review
Chemistry, Multidisciplinary
Uttam Kumer Roy, Birthe Vejby Nielsen, John James Milledge
Summary: Microalgae are an attractive natural source of diverse biomolecules, including enzymatic and non-enzymatic antioxidants, but economically sustainable production remains challenging. The main hurdles include increasing yield, achieving optimal cultivation conditions, energy-efficient downstream processing, and optimal storage of antioxidant molecules. This review discusses strategies to enhance antioxidant accumulation in cells and the opportunities and limitations of current technologies for antioxidant enzymes production from microalgae biomass.
APPLIED SCIENCES-BASEL
(2021)
Article
Integrative & Complementary Medicine
David Neglo, Clement Okraku Tettey, Edward Ken Essuman, Justice Dziedzorm Amenu, Felix Charles Mills-Robertson, Daniel Sedohia, Adjoa Agyemang Boakye, Daniel A. Abaye
Summary: This study investigated the influence of Annona muricata extracts on MRSA and found that different parts of the plant contained varying proportions of secondary metabolites with varied antimicrobial activities. It was observed that some extracts antagonized the activity of ampicillin against MRSA while others potentiated it. Additionally, the extracts significantly potentiated the efficacy of streptomycin and tetracycline against MRSA, with the aqueous root extract showing the highest synergistic effect.
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
(2021)
Article
Chemistry, Multidisciplinary
Birthe Vejby Nielsen, Supattra Maneein, Jahanvi Dipakbhai Anghan, Riya Mukeshbhai Anghan, Md Mahmud Al Farid, John James Milledge
Summary: Sargassum muticum is an invasive species with negative impacts on the coasts of the British Isles, mainland Europe and North America. The commercial use of these biomasses may help alleviate the costs of removal and control, and they can be effective in removing aqueous cationic dyes.
APPLIED SCIENCES-BASEL
(2022)
Article
Biochemistry & Molecular Biology
Daniel E. K. Kabotso, David Neglo, Pius Kwashie, Irene A. Agbo, Daniel A. Abaye
Summary: The study found that lemongrass essential oil exhibited the strongest inhibitory effect on MRSA growth and enhanced the activity of three antibiotics, mainly attributed to citral and geranyl acetate.
CHEMISTRY & BIODIVERSITY
(2022)
Review
Chemistry, Multidisciplinary
Daniel A. Abaye, Irene A. Agbo, Birthe V. Nielsen
Summary: This article discusses the use of supercharging reagents in ESI-MS to improve signal intensity and ion generation mechanisms, examining their modes of action, revisiting the three main theories of ESI mechanisms, listing confirmed supercharging reagents, and exploring additional potential candidates. Advances in technology, ion source design, analyzers, and computer modeling are opening up new avenues for research in polymer science.
Article
Biotechnology & Applied Microbiology
Uttam K. Roy, Birthe Nielsen, John J. Milledge
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
(2020)
Review
Biotechnology & Applied Microbiology
Birthe Nielsen, Supattra Maneein, Md Mahmud Al Farid, John J. Milledge
FERMENTATION-BASEL
(2020)
Review
Biotechnology & Applied Microbiology
Supattra Maneein, John J. Milledge, Birthe V. Nielsen, Patricia J. Harvey
FERMENTATION-BASEL
(2018)
Article
Spectroscopy
Birthe V. Nielsen, Daniel A. Abaye, Minh T. L. Nguyen
MASS SPECTROMETRY LETTERS
(2017)