Article
Microbiology
Anil K. Persad, Hanan A. Fahmy, Nicholas Anderson, Jing Cui, Zeynal Topalcengiz, Saharuetai Jeamsripong, Patrick M. Spanninger, Robert L. Buchanan, Kalmia E. Kniel, Michele T. Jay-Russell, Michelle D. Danyluk, Gireesh Rajashekara, Jeffrey T. LeJeune
Summary: This study demonstrates that MALDI-TOF is a viable alternative for the rapid identification and differentiation of Salmonella serovars.
Article
Chemistry, Multidisciplinary
Ouyang Dan, Chuanzhe Wang, Chao Zhong, Juan Lin, Gang Xu, Guane Wang, Zian Lin
Summary: In this study, novel LDI-MS substrates were designed to enhance the sensitivity of mass signals and achieve high accuracy diagnosis of different saccharides and central precocious puberty (CPP).
Article
Biotechnology & Applied Microbiology
R. Gathercole, E. Tranfield, D. Xia, G. Perez-Cordon, G. Robinson, D. Timofte, F. Zendri, R. M. Chalmers
Summary: The study developed a method to purify Cryptosporidium spp. oocysts from clinical stool samples and evaluated them using mass spectrometry. The method produced distinct spectra specific to different Cryptosporidium species, demonstrating the utility of the purification method.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Review
Microbiology
Xin-Fei Chen, Xin Hou, Meng Xiao, Li Zhang, Jing-Wei Cheng, Meng-Lan Zhou, Jing-Jing Huang, Jing-Jia Zhang, Ying-Chun Xu, Po-Ren Hsueh
Summary: MALDI-TOF MS has been used in clinical microbiology since 2010, offering advantages such as convenience, speed, accuracy, and low cost. It has shown promising results in rapid detection of highly virulent and drug-resistant pathogens, making it a valuable tool in clinical microbial identification.
Article
Chemistry, Analytical
Liping Liu, Yaru Jia, Shanxia Jin, Xiangdong Chen, Ping Zhou
Summary: A new strategy for the analysis of small biomolecules and drugs by LDI MS is presented, using in situ polymerized poly(L-dopa) film on standard stainless steel MALDI plate. This method has high sensitivity and good salt tolerance, allowing detection of amino acids even in saturated salt solution, and has the potential to be a practical solution for small biomolecule analysis.
MICROCHEMICAL JOURNAL
(2023)
Article
Multidisciplinary Sciences
Chadaporn Chaiden, Janthima Jaresitthikunchai, Anusak Kerdsin, Nattakan Meekhanon, Sittiruk Roytrakul, Suphachai Nuanualsuwan
Summary: In this study, MALDI-TOF-MS analysis of extracted peptides was developed to improve the classification of S. suis serotypes. The method successfully differentiated highly pathogenic SS2 and SS14 from other serotypes, indicating its potential as an alternative approach for serotyping clinically pathogenic S. suis isolates.
Article
Biochemical Research Methods
Xin Liu, Taojunfeng Su, Yen-Michael S. Hsu, Hua Yu, He Sarina Yang, Li Jiang, Zhen Zhao
Summary: The study demonstrates that MALDI-TOF MS combined with machine-learning algorithms can be used to develop a rapid and user-friendly method for differentiating MRSA from MSSA. This cost-effective and time-efficient alternative to conventional resistance detection in routine microbiology laboratories suggests an improvement in clinical treatment in the future.
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2021)
Article
Chemistry, Analytical
Ge Ma, Xiaoyong Zhao, Mengzhe Guo, Yaqin Liu, Keqiang Shi, Cheng Guo, Yuanjiang Pan
Summary: In this study, a matrix glycosylation strategy was proposed to improve the sensitivity and signal reproducibility of MALDI MS analysis. The glycosylated matrices, especially 6-GAQ, exhibited remarkably-improved performance in detecting metabolites and imaging small molecules in complex samples. The glycosylation strategy showed great potential in the field of mass spectrometry.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Xufang Hu, Zongping Wang, Haolin Chen, An Zhao, Nianrong Sun, Chunhui Deng
Summary: An advanced metabolic analysis tool based on highly porous metal oxides derived from metal-organic frameworks (MOFs) is developed in this study. The tool successfully differentiates and stages renal cell carcinoma (RCC), as well as identifies the size of tumors. This achievement shows promising prospects for designer matrix-based analytical tools in disease diagnosis.
ANALYTICAL CHEMISTRY
(2022)
Article
Agriculture, Multidisciplinary
Fang Lin, Winnie C. Soko, Jing Xie, Hongyan Bi
Summary: This study aims to evaluate fish muscle exudate proteins to discover potential allergens for allergy tests. Magnetic beads modified with an IgE antibody were used to isolate allergens in the exudate sample. Eight allergens from large yellow croaker exudate were successfully identified. The proposed microfluidic-magnetic-based allergen extraction protocol provides a facile and rapid test for seafood allergies, addressing food safety issues for allergic populations.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Chemistry, Applied
Zhaoliang Chai, Hongyan Bi
Summary: In this study, anti-E. coli functionalized magnetic beads were constructed and successfully used to capture E. coli from fish sarcoplasmic protein and fish muscle protein extracts. The reproducibility and accuracy of the method were demonstrated through experiments. The method showed high specific detection of target bacteria in complex biological samples.
Review
Chemistry, Multidisciplinary
Qingli Zeng, Meng-Chan Xia, Xinchi Yin, Simin Cheng, Zhichao Xue, Siyuan Tan, Xiaoyun Gong, Zihong Ye
Summary: The variation among individual cells has a significant impact on biological functions, and single-cell analysis using mass spectrometry provides unique insights into intricate biochemical mechanisms. However, measuring at the unicellular level is challenging due to the complex composition of cells, small substance quantities, and differences in compound concentrations. Recent advancements in ionization techniques in single-cell mass spectrometry have improved the sensitivity and identification abilities of the method.
FRONTIERS IN CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Govinda Mandal, Lukas Moran, Lukas Pecinka, Petr Vanhara, Josef Havel
Summary: This study demonstrates that supplementation of common MALDI matrices with black phosphorus particles can enhance the intensity and reproducibility of mass spectra acquired from intact cells. Black phosphorus particles interact with aromatic groups within molecules, enhancing the intensity of mass spectra of amino acids and peptides. Furthermore, black phosphorus particles induce the formation of crystals of the matrix and analyte on the target plate.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Analytical
Jun-Qiao Wang, Jing Zhao, Shao-Ping Nie, Ming-Yong Xie, Shao-Ping Li
Summary: Mass spectrometry has been used for high-sensitivity structural determination of carbohydrates, but the correlation between carbohydrate structure and mass spectra is still insufficient. In this study, various trisaccharides and cyclodextrins were investigated using MALDI TOF/TOF MS, and diagnostic fragment ions were successfully used for the characterization of polysaccharides with different structural features.
ANALYTICAL LETTERS
(2023)
Article
Infectious Diseases
Margarita Estreya Zvezdanova, Pilar Escribano, Jesus Guinea, Patricia Munoz, David Rodriguez-Temporal, Belen Rodriguez-Sanchez
Summary: The implementation of commercial kits for standardized fungal sample preparation and updated reference libraries enables the easy use of MALDI-TOF MS for routine identification of fungal species. Although further improvement in the databases is needed, this method allows for the correct identification of a significant number of fungal species at the species level.
Article
Biotechnology & Applied Microbiology
R. Martzy, T. M. Mello-de-Sousa, R. L. Mach, D. Yaver, A. R. Mach-Aigner
Summary: The study reveals that spontaneous degeneration of Trichoderma reesei strains during large-scale cultivation can lead to a loss of cellulase formation. The chromatin condensation in relevant promoter regions, related to the production capacity of the strain, plays a role in the occurrence of degenerated strain population.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Dermatology
Michaela Sochorova, Katerina Vavrova, Maria Fedorova, Zhixu Ni, Denise Slenter, Martina Kutmon, Egon L. Willighagen, Sophia Letsiou, Daniel Torocsik, Martina Marchetti-Deschmann, Samuele Zoratto, Christopher Kremslehner, Florian Gruber
Summary: Lipids play crucial roles in maintaining the epidermal barrier, regulating skin physical properties, and defending against microbes in the skin. The complexity of lipidomics analysis is comparable to that of proteomics, and recent technological advancements have enabled researchers to better understand the specific roles of lipid species in skin biology.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2022)
Article
Green & Sustainable Science & Technology
Laura Daza-Serna, Katarina Knezevic, Norbert Kreuzinger, Astrid Rosa Mach-Aigner, Robert Ludwig Mach, Joerg Krampe, Anton Friedl
Summary: This study assessed the use of electrodialysis for the purification of synthetic erythritol culture broth and the recovery of salts, providing an efficient alternative to minimize waste generation in erythritol production. Electrodialysis demonstrated potential in removing ions from the synthetic culture broth and further recovering them into a concentrated fraction, with low erythritol losses and energy consumption.
Article
Biotechnology & Applied Microbiology
Johanna Kreuter, Georg Stark, Robert L. Mach, Astrid R. Mach-Aigner, Christian Zimmermann
Summary: In this study, a CRISPR/Cas9-based genome editing method using RNPs was developed for single and multiplex genome editing in Aureobasidium spp., with improved homologous recombination rate and efficient editing capabilities.
JOURNAL OF BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Mouhamadou M. Dieng, Kiswend-sida M. Dera, Percy Moyaba, Gisele M. S. Ouedraogo, Guler Demirbas-Uzel, Fabian Gstottenmayer, Fernando C. Mulandane, Luis Neves, Sihle Mdluli, Jean-Baptiste Rayaisse, Adrien M. G. Belem, Soumaila Pagabeleguem, Chantel J. de Beer, Andrew G. Parker, Jan Van den Abbeele, Robert L. Mach, Marc J. B. Vreysen, Adly M. M. Abd-Alla
Summary: The sterile insect technique is an effective and sustainable method for managing insect pests. This study examines the interaction between Sodalis bacteria and Trypanosoma infection in tsetse flies. The presence of Sodalis and Trypanosoma varied among different countries and tsetse species. The study also found differences in the prevalence of specific Trypanosoma species in different regions.
SCIENTIFIC REPORTS
(2022)
Article
Food Science & Technology
Barbara Streit, Tibor Czabany, Georg Weingart, Martina Marchetti-Deschmann, Shreenath Prasad
Summary: This article presents a method for the extraction and quantification of OTA and OT alpha in pig and poultry matrices. The samples are extracted using either ethyl acetate/sodium hydrogencarbonate/ethyl acetate or an acetonitrile/water mixture, and quantitative analysis is performed using LC-MS/MS. The method validation demonstrates good linearity, accuracy, and precision.
Article
Biochemistry & Molecular Biology
Stephanie Steinberger, Sobha Karuthedom George, Lucia Laukova, Rene Weiss, Carla Tripisciano, Martina Marchetti-Deschmann, Viktoria Weber, Gunter Allmaier, Victor U. Weiss
Summary: Extracellular vesicles (EVs) have potential applications in the medical field, and their stability during isolation and purification is crucial. This study investigated EV stability and particle numbers using nES GEMMA and NTA techniques, and found that nES GEMMA can provide important information on EV stability.
Article
Parasitology
Mouhamadou M. Dieng, Antonios A. Augustinos, Guler Demirbas-Uzel, Vangelis Doudoumis, Andrew G. Parker, George Tsiamis, Robert L. Mach, Kostas Bourtzis, Adly M. M. Abd-Alla
Summary: This study assessed the interaction between GpSGHV and tsetse endosymbionts and found that Wolbachia may have a protective role against GpSGHV, indicating a dynamic and species-specific interaction between the tsetse host and its associated microbes.
PARASITES & VECTORS
(2022)
Article
Entomology
Hanano Yamada, Benewende Aristide Kabore, Nanwintoum Severin Bimbile Somda, Nonhlanhla L. L. Ntoyi, Chantel Janet de Beer, Jeremy Bouyer, Carlos Caceres, Robert L. L. Mach, Yeudiel Gomez-Simuta
Summary: The sterile insect technique (SIT) is an environmentally friendly and species-specific method of pest control that involves mass production of target insects in a factory and sterilizing them using irradiation, typically gamma rays. As gamma sources become more difficult and expensive to obtain, and regulations surrounding their use become stricter, there is growing interest in alternative irradiation methods such as X-rays. A recent study found that X-rays can be used effectively to sterilize major SIT target insect species, making X-ray units suitable for small-to-medium-sized SIT programs.
Article
Virology
Samuele Zoratto, Thomas Heuser, Gernot Friedbacher, Robert Pletzenauer, Michael Graninger, Martina Marchetti-Deschmann, Victor U. Weiss
Summary: Gas-phase electrophoresis on a nano-Electrospray Gas-phase Electrophoretic Mobility Molecular Analyzer (nES GEMMA) separates single-charged, native analytes based on surface-dry particle size. By using ammonium acetate as the electrolyte, nES GEMMA has been used to investigate (bio-)nanoparticle containing samples and analyze their composition, size, distribution, and numbers. This study focused on pH changes on adeno-associated virus 8 (AAV8) based virus-like particles (VLPs) and found that filled VLPs exhibit differences in diameter and aggregation depending on the pH of the electrolyte solution used for electrospraying.
Article
Engineering, Biomedical
Martin Handelshauser, You-Rong Chiang, Martina Marchetti-Deschmann, Philipp J. Thurner, Orestis G. Andriotis
Summary: Collagen fibrils are the fundamental structural units that provide mechanical properties to tissues. In this study, a nonlinear rheological Maxwell model was used to describe the viscoelastic behavior of collagen fibrils. The model was fitted to experimental data and showed that the tensile modulus increased with decreasing hydration, while the viscosity was marginally affected by hydration but dependent on strain rate, indicating thixotropic behavior at low strain rates.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2023)
Article
Microbiology
Kristina Missbach, Daniel Flatschacher, Christoph Bueschl, Jonathan Matthew Samson, Stefan Leibetseder, Martina Marchetti-Deschmann, Susanne Zeilinger, Rainer Schuhmacher
Summary: Many studies have neglected the influence of light on fungal secondary metabolite production. In this study, the effect of different light regimes on Trichoderma atroviride secondary metabolite production was systematically determined using metabolomics analysis. It was found that reduced light conditions maximize secondary metabolite production.
Article
Chemistry, Multidisciplinary
Jessica Pichler, Rosa Maria Eder, Lukas Widder, Markus Varga, Martina Marchetti-Deschmann, Marcella Frauscher
Summary: With the EU aiming for net-zero greenhouse gas emissions by 2050, transforming conventional production cycles into cradle-to-cradle approaches is necessary. Spent coffee grounds, usually disposed of in landfills, have the potential to be used as high-quality feedstock for biofuel or bio-lubricant production. The properties of spent coffee grounds oil (SCGO) were investigated and compared to a reference polyalphaolefin (PAO 8), revealing differences in physicochemical properties and oxygen content. The tribological behavior of SCGO as a lubricant base oil or as an additive in PAO 8 was studied, showing superior friction-reducing effects compared to pure PAO 8 on certain surfaces.
GREEN CHEMISTRY LETTERS AND REVIEWS
(2023)
Review
Chemistry, Multidisciplinary
Jessica Pichler, Rosa Maria Eder, Charlotte Besser, Lucia Pisarova, Nicole Doerr, Martina Marchetti-Deschmann, Marcella Frauscher
Summary: In recent years, there has been a shift towards greener lubrication, driven by policy initiatives and sustainability goals. The composition of the lubricant, particularly the type of base oil, plays a crucial role in its sustainability and environmental impact. Therefore, there is a growing demand for environmentally friendly lubricant alternatives. This review investigates bio-based, bio-derived, and other sustainable lubricant components that can replace conventional petroleum-based formulations in line with green chemistry and tribology principles.
GREEN CHEMISTRY LETTERS AND REVIEWS
(2023)
Article
Genetics & Heredity
Matthias Schmal, Crystal Girod, Debbie Yaver, Robert L. Mach, Astrid R. Mach-Aigner
Summary: With the advent of affordable Next-Generation Sequencing technologies, there has been a drastic increase in the number of known non-protein coding RNAs. These non-coding RNAs play important roles in gene expression regulation at various levels, from RNA-RNA to RNA-DNA and RNA-protein interactions. Predicting and analyzing ncRNAs can be challenging, requiring the use of multiple bioinformatics tools. To address this, researchers have developed the analysis pipeline Pinc, which allows for the prediction and identification of ncRNAs, as well as differential expression analysis.
NAR GENOMICS AND BIOINFORMATICS
(2022)