Review
Biochemical Research Methods
Lanaia Itala Louzeiro Maciel, Ricardo Alves Bernardo, Rafael Oliveira Martins, Almir Custodio Batista Jr, Joao Victor Ataide Oliveira, Andrea Rodrigues Chaves, Boniek Gontijo Vaz
Summary: The imaging of biological tissues using mass spectrometry imaging (MSI) techniques allows for the visualization of the distribution of various metabolites, drugs, lipids, and glycans in complex samples. MSI methods like DESI-MSI and MALDI-MSI provide high sensitivity and the ability to evaluate/visualize multiple analytes in a single sample, overcoming limitations of classical microscopy techniques. This review discusses the application of DESI-MSI and MALDI-MSI in evaluating exogenous and endogenous molecules in biological samples, providing technical insights and recent research findings.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Alena N. Joignant, Hongxia Bai, Jacob P. Guymon, Kenneth P. Garrard, Mark Pankow, David C. Muddiman
Summary: This article presents the development and characterization of the NextGen infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) instrument, including the development of a new analysis geometry. The study demonstrates comparable results between vertically oriented transmission mode (tm)-IR-MALDESI and reflection mode (rm)-IR-MALDESI on mouse tissue. It also examines the use of glycerol as an alternative matrix, which shows little improvement in ion abundances for tm-IR-MALDESI.
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2022)
Article
Chemistry, Analytical
Joseph H. H. Holbrook, Emily R. R. Sekera, Arbil Lopez, Brian D. D. Fries, Fernando Tobias, Kubra Akkaya, Maria M. M. Mihaylova, Amanda B. B. Hummon
Summary: Lipids are essential macromolecules that play crucial roles in numerous biological events. Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is a powerful tool for understanding the spatial localization of lipids in biological systems. In this study, the use of ammonium fluoride (NH4F) as a comatrix additive was found to enhance lipid detection in biological samples, with a signal increase of up to 200%. The addition of NH4F was observed to enhance the lipid signal of [M-H](-) ions, attributed to a proton transfer reaction in various lipid classes. Overall, the study shows that the NH4F comatrix additive significantly improves sensitivity for lipid detection in a MALDI system and can be applied to various applications.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Ouyang Dan, Chuanzhe Wang, Chao Zhong, Juan Lin, Gang Xu, Guane Wang, Zian Lin
Summary: In this study, novel LDI-MS substrates were designed to enhance the sensitivity of mass signals and achieve high accuracy diagnosis of different saccharides and central precocious puberty (CPP).
Article
Biochemical Research Methods
Vishnu Shankar, Robert Tibshirani, Richard N. Zare
Summary: DESI-MSI has been increasingly used for detecting metabolites and lipids from human tissues and biofluids, but accurate computational and statistical methods are necessary to identify significant differences. MassExplorer is a flexible tool that helps researchers preprocess data, visualize structures, and select statistically significant metabolites.
Article
Chemistry, Analytical
G. Asher Newsome, Kathleen M. Martin
Summary: This study examines selected Native American woven woodsplint basketry created between 1870 and 1983 to recover traditional knowledge on their manufacture by identifying dyes or colorants. An ambient mass spectrometry system is used to non-invasively sample from intact objects, with no cutting or exposure to liquid. The analysis process involves thermal desorption, gas phase analysis, and photoionization for real-time identification of dye molecules.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Sangwon Cha, Gyouwoong Jun, Yougyeong Park, Sung Jun An, Donghoon Lee
Summary: Nanospray desorption electrospray ionization (nano-DESI) was used to analyze the metabolite fingerprints on Hypericum leaves. The intensity changes of key metabolites over time were observed through full mass scan experiments and monitored using selected ion monitoring and pseudo-selected reaction monitoring experiments. The correlation between the time-dependent changes in the metabolite fingerprints and depth-wise metabolite distribution was examined by removing the surface layers of the leaves multiple times.
Article
Biochemical Research Methods
Lieke Lamont, Darya Hadavi, Brent Viehmann, Bryn Flinders, Ron M. A. Heeren, Rob J. Vreeken, Tiffany Porta Siegel
Summary: Mass spectrometry imaging (MSI) is widely used in the pharmaceutical industry, but quantitative mass spectrometry imaging (Q-MSI) faces challenges due to biological sample variability and limited sample cleanup strategies. This study found that multiple reaction monitoring (MRM) mode on a triple quadrupole mass spectrometer (QqQ) provided the best performance for quantitative detection of drug candidates A and B.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Fengjian Chu, Gaosheng Zhao, Wangyu Li, Wei Wei, Weiwei Chen, Zihan Ma, Zhan Gao, Nazifi Sani Shuaibu, Jikui Luo, Bingwen Yu, Hongru Feng, Yuanjiang Pan, Xiaozhi Wang
Summary: A microwave plasma torch-based ion/molecular reactor (MPTIR) has been developed for creating and monitoring rapid oxidation reactions without the addition of external oxidants or catalysts. The oxidation products from the MPTIR match those of the long-term environmental oxidative process, and two unreported environmental compounds were identified in actual water samples using this device.
ANALYTICAL CHEMISTRY
(2022)
Article
Polymer Science
Lidia Molina-Millan, Aljoscha Koerber, Bryn Flinders, Berta Cillero-Pastor, Eva Cuypers, Ron M. A. Heeren
Summary: Synthetic polymers, when improperly disposed of, accumulate in the environment and pose potential health risks by degrading into micro- and nanoparticles (MNPs). Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) can be used to study biological or toxicological changes in organisms exposed to MNPs. MALDI-2 has been found to enhance the sensitivity of polymer analysis, making it a promising tool for studying the distribution of polymers in biological systems.
Review
Chemistry, Analytical
Theoneste Muyizere, Janvier Mukiza
Summary: This review summarizes the application of metal ion-assisted ionization sources in the mass spectrometric analysis of polymers and discusses different ionization systems. It also provides an outlook on the future research trends and challenges in this field.
ANALYTICAL METHODS
(2022)
Article
Biochemical Research Methods
Juha-Pekka Hieta, Nina Sipari, Heikki Raikkonen, Markku Keinanen, Risto Kostiainen
Summary: This study demonstrates the potential of infrared laser ablation atmospheric pressure photoionization mass spectrometry (LAAPPI-MS) in analyzing the substructures of Arabidopsis thaliana leaves and its application in depth profiling analysis. The results show that LAAPPI-MS can detect variations in the distribution of various metabolites among different substructures of A. thaliana leaves.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Biochemical Research Methods
Gwangbin Lee, Sangwon Cha
Summary: Nano-DESI MS has been explored in the forensic chemical analysis of ink directly from handwriting on paper, showing the potential for changing mass spectral profiles over time to reflect the differential distribution of analytes in a vertical direction. The study confirmed the capability of the depth-dependent analysis of nano-DESI MS.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Biochemistry & Molecular Biology
Margaux Fresnais, Siwen Liang, Deniz Seven, Nevena Prodanovic, Julia Sundheimer, Walter E. Haefeli, Juergen Burhenne, Remi Longuespee
Summary: The development and validation of desorption/ionization mass spectrometric assays for drug quantification in tissue sections is crucial for their widespread application in (clinical) pharmacology. Advances in desorption electrospray ionization have demonstrated its usefulness for developing targeted quantification methods that meet validation requirements. Factors such as desorption spot morphology, analytical time, and sample surface need to be considered for successful method development. This study highlights the importance of considering desorption kinetics during DESI analyses, which can help reduce analytical time, verify solvent-based drug extraction methods, and predict feasibility of imaging assays. These findings provide valuable guidance for future development of validated DESI-profiling and imaging methods.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Medicinal
Margaux Fresnais, Siwen Liang, Marius Breitkopf, Joshua Raoul Lindner, Emmanuelle Claude, Steven Pringle, Pavel A. Levkin, Konstantin Demir, Julia Benzel, Julia Sundheimer, Britta Statz, Kristian W. Pajtler, Stefan M. Pfister, Walter E. Haefeli, Juergen Burhenne, Remi Longuespee
Summary: Desorption/ionization-mass spectrometric methods are advantageous for the analysis of solid biological matrices. The development of drug quantification assays using DESI has been challenging, but new prototype source setups show promise for targeted quantitative analyses.
