Journal
ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES
Volume 28, Issue 11, Pages 1545-1550Publisher
ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOC
DOI: 10.5713/ajas.15.0425
Keywords
Berkshire; Meat Quality Traits; v-Myelocytomatosis Viral Oncogene Homologue; Single Nucleotide Polymorphism; Association Analysis
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Funding
- Next-Generation BioGreen 21 Program [PJ01110901]
- Rural Development Administration, Republic of Korea
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c-MYC (v-myelocytomatosis viral oncogene homologue) is a transcription factor that plays important role in many biological process including cell growth and differentiation, such as myogenesis and adipogenesis. In this study, we aimed to detect MYC gene polymorphisms, their genotype frequencies and to determine associations between these polymorphisms and meat quality traits in Berkshire pigs. We identified a single nucleotide polymorphism (SNP) in intron 2 of MYC gene by Sanger sequencing, i.e., g.3350G>C (rs321898326), that is only found in Berkshire pigs, but not in other breeds including Duroc, Landrace, and Yorkshire pigs that were used in this study. Genotypes of total 378 Berkshire pigs (138 sows and 240 boars) were determined using Hha I restriction enzyme digestion after polymerase chain reaction. Observed allele frequencies of GG, GC, and CC genotypes were 0.399, 0.508, and 0.093 respectively. Statistical analysis indicated that the g.3350G>C polymorphism was significantly associated with pH(45min) and cooking loss (p<0.05), suggesting that g.3350G>C SNP can be used for pre-selection of pH(45min) and cooking loss traits in Berkshire pigs.
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