Article
Chemistry, Analytical
Hideo Utsumi, Toshiki Masumizu, Ryoma Kobayashi, Tomoko Tahira, Fuminori Hyodo, Tatsuya Shimizu, Tatsuya Naganuma, Kazunori Anzai
Summary: Free radicals are key substances in organisms but can also cause oxidative diseases. Dynamic nuclear polarization- magnetic resonance imaging is a noninvasive imaging method that can be used for in vivo free radical imaging.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Miho C. Emoto, Hideo Sato-Akaba, Naoya Hamaue, Katsuya Kawanishi, Hisashi Koshino, Shun Shimohama, Hirotada G. Fujii
Summary: Alzheimer's disease is characterized by progressive cognitive decline and deposition of amyloid-beta peptides. Oxidative stress induced by reactive oxygen species is a prominent feature in AD. Noninvasive imaging using EPR technology can detect early changes in brain redox status, providing valuable insights into disease progression.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Simona Mrakic-Sposta, Maristella Gussoni, Mauro Marzorati, Simone Porcelli, Gerardo Bosco, Costantino Balestra, Michela Montorsi, Claudio Lafortuna, Alessandra Vezzoli
Summary: Exposure to acute normobaric hypoxia (NH) leads to reactive oxygen species (ROS) accumulation, which can cause oxidative damage. This study investigated the production kinetics of ROS and its impact on oxidative damage. Nine subjects were monitored for ROS production and oxidative damage markers in blood and urine. The results showed a peak ROS production at 4 hours, and oxidative damage markers increased at 4 hours and 1 hour after hypoxia offset. These reversible phenomena were SpO(2)-dependent. The experimental model could be useful for evaluating acclimatization level, especially in mountain rescues involving technical/medical workers with limited acclimatization time, such as helicopter flights.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Juvinch R. Vicente, Amanda Durkin, Kristina Shrestha, Mihaela Balu
Summary: Melanin plays a significant role in skin homeostasis and protection. The two-photon excited fluorescence lifetime detection method allows for selective and quantitative measurement of melanin. Previous studies lacked consideration of skin heterogeneity due to limited field of view. In this work, a fast large-area multiphoton exoscope was used to evaluate the 3D distribution of epidermal melanin content, improving measurement reliability.
SCIENTIFIC REPORTS
(2022)
Review
Clinical Neurology
Christopher Hollen, Lee E. Neilson, Ramon F. Barajas, Ian Greenhouse, Rebecca I. Spain
Summary: Conventional MRI is important for evaluating patients with multiple sclerosis, but its role in detecting the underlying pathophysiology of neurodegeneration is limited. Biomarkers that identify processes underlying neurodegeneration before irreversible damage occurs are needed. Oxidative stress is believed to play a major role in the pathogenesis of multiple neurodegenerative diseases, including multiple sclerosis. Non-invasive imaging techniques offer real-time assessment of oxidative stress within the brain, which is an area of unrealized potential.
FRONTIERS IN NEUROLOGY
(2023)
Review
Biochemistry & Molecular Biology
Yoichi Takakusagi, Ryoma Kobayashi, Keita Saito, Shun Kishimoto, Murali C. Krishna, Ramachandran Murugesan, Ken-ichiro Matsumoto
Summary: New imaging modalities are being developed for imaging tumor microenvironments. Electron Paramagnetic Resonance Imaging (EPRI) is unique in its ability to provide quantitative images of oxygenation. Dynamic nuclear polarization (DNP) opens new capabilities in molecular imaging.
Article
Chemistry, Multidisciplinary
Toshinobu Nakajo, Jun Kumagai, Shinpei Kusaka, Akihiro Hori, Yuh Hijikata, Jenny Pirillo, Yunsheng Ma, Ryotaro Matsuda
Summary: Triplet carbenes (TCs) are highly reactive species with unique electronic states, making it challenging to balance reactivity and stability. By confining TCs in the nanospace of a metal-organic framework (MOF), it is possible to enhance their thermal stability while maintaining reactivity, allowing for the stabilization of highly reactive species.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Review
Biochemistry & Molecular Biology
Shuo Huang, Rui Dai, Zhiqi Zhang, Han Zhang, Meng Zhang, Zhangjun Li, Kangrui Zhao, Wenjun Xiong, Siyu Cheng, Buhua Wang, Yi Wan
Summary: CRISPR/Cas systems are widely used in gene editing due to their accuracy, programmability, ease of use, and affordability. Additionally, they have been applied in live-cell imaging and bioanalysis, expanding the scope of their use beyond gene editing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Paola Zimmermann Crocomo, Masato Okazaki, Takumi Hosono, Satoshi Minakata, Youhei Takeda, Przemyslaw Data
Summary: This study focuses on the synthesis and application of donor-acceptor-donor molecules based on dibenzophenazine acceptor group, as well as the characterization of charge carrier species and electropolymerisation properties through UV-Vis-NIR spectroelectrochemistry and potentiostatic EPR techniques. The promising electrochromic features of both small molecules and conjugated polymers led to the assembly and investigation of electrochromic devices, showcasing high efficiencies in OLEDs with EQEs up to 20.5% and luminance values up to 85,000 cd/m².
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Article
Chemistry, Inorganic & Nuclear
Anthony J. Campanella, Manh-Thuong Nguyen, Jun Zhang, Thacien Ngendahimana, William E. Antholine, Gareth R. Eaton, Sandra S. Eaton, Vassiliki-Alexandra Glezakou, Joseph M. Zadrozny
Summary: This work investigates how variations in ligand structures affect the EPR spectroscopic properties of five Cr(III) complexes, indicating that the spectral broadening is correlated to rigidity in the inner coordination sphere and reflected in ligand-dependent distribution of Cr-N bond distances.
DALTON TRANSACTIONS
(2021)
Article
Biochemistry & Molecular Biology
Boris Epel, Joseph P. Y. Kao, Sandra S. Eaton, Gareth R. Eaton, Howard J. Halpern
Summary: Recent advances in instrumentation and molecular probes have made it possible to quantitatively image tissue redox status in vivo. The use of low-frequency electron paramagnetic resonance (EPR) techniques and novel spin probes has led to significant advancements in this field. However, further improvements in spin probes and RS instrumentation are needed to fully realize the potential of redox imaging in tissue.
ANTIOXIDANTS & REDOX SIGNALING
(2023)
Article
Chemistry, Analytical
Anatoly R. Melnikov, Samat B. Zikirin, Evgeny V. Kalneus, Vladimir I. Ivannikov, Yuri A. Grishin, Oleg A. Anisimov
Summary: In this study, the sensitivity of three different types of PVDF standard pyroelectric films with different coatings was investigated to detect EPR signals, with the gold-coated PVDF film shown to be the most promising for this application. Comparative analysis of noise-equivalent power and specific detectivity from experimental spectra determined the effectiveness of the PVDF films for indirect detection of EPR signals. Additionally, an estimation was made on whether the sensitivity of a PVDF-based pyrodetector is sufficient for this purpose.
Article
Biochemistry & Molecular Biology
Taiming Liu, Meijuan Zhang, Avoumia Mourkus, Hobe Schroeder, Lubo Zhang, Gordon G. Power, Arlin B. Blood
Summary: Chronic hypoxia at high altitude alters iron homeostasis and bioactive nitric oxide metabolites (NOx) levels in the fetal circulation. The fetus utilizes iron from non-hematopoietic tissues for erythropoiesis and shows increased bioavailability of NO in response to hypoxic stress at high altitude during gestation.
Article
Chemistry, Multidisciplinary
Rachel Wills, Jonathan Farhi, Patrick Czabala, Sophia Shahin, Jennifer M. Spangle, Monika Raj
Summary: Aliphatic aldehydes are reactive electrophilic carbonyls that cross-link with DNA and proteins leading to cellular toxicity and disease pathogenesis. Chemical sensors that generate a benzimidazole moiety upon reaction with aliphatic aldehydes of different chain lengths have been developed, enabling the real-time imaging and quantification of aldehyde formation in live mammalian cells. This tool has the potential to revolutionize aldehyde research by uncovering cellular metabolites that have previously remained elusive.
Article
Chemistry, Multidisciplinary
Yi-Nan Liu, Zhen-Ting Lv, Wen-Li Lv, Dong-Feng Liu, Xian-Wei Liu
Summary: We developed a label-free and noninvasive imaging method to measure electron transfer in microbial cells. By imaging the redox reaction of cytochromes in microbial cells, we obtained the electrochemical activity parameters of individual cells, revealing heterogeneities in electron transfer at the single-cell level.
Review
Biochemistry & Molecular Biology
Nikolay Polyakov, Tatyana Leshina, Lidiya Fedenok, Irina Slepneva, Igor Kirilyuk, Justyna Furso, Magdalena Olchawa, Tadeusz Sarna, Martyna Elas, Itzhak Bilkis, Lev Weiner
ANTIOXIDANTS & REDOX SIGNALING
(2018)
Article
Biochemistry & Molecular Biology
Ewa Podgorska, Agnieszka Drzal, Zenon Matuszak, Jan Swakon, Andrzej Slominski, Martyna Elas, Krystyna Urbanska
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2018)
Article
Oncology
Marcin Luty, Katarzyna Piwowarczyk, Anna Labedz-Maslowska, Tomasz Wrobel, Malgorzata Szczygiel, Jessica Catapano, Grazyna Drabik, Damian Ryszawy, Sylwia Kedracka-Krok, Zbigniew Madeja, Maciej Siedlar, Martyna Elas, Jaroslaw Czyz
Article
Computer Science, Hardware & Architecture
M. Gonet, B. Epel, M. Elas
COMPUTERS & ELECTRICAL ENGINEERING
(2019)
Article
Multidisciplinary Sciences
Katarzyna Jasinska-Konior, Olga Wiechec, Michal Sarna, Agnieszka Panek, Jan Swakon, Marta Michalik, Krystyna Urbanska, Martyna Elas
SCIENTIFIC REPORTS
(2019)
Article
Multidisciplinary Sciences
Anna Markiewicz, Anna A. Brozyna, Ewa Podgorska, Martyna Elas, Krystyna Urbanska, Anton M. Jetten, Andrzej T. Slominski, Wojciech Jozwicki, Jolanta Orlowska-Heitzman, Grzegorz Dyduch, Bozena Romanowska-Dixon
SCIENTIFIC REPORTS
(2019)
Article
Biochemistry & Molecular Biology
Michal Gonet, Boris Epel, Howard J. Halpern, Martyna Elas
CELL BIOCHEMISTRY AND BIOPHYSICS
(2019)
Article
Multidisciplinary Sciences
Malwina Karwicka, Barbara Pucelik, Michal Gonet, Martyna Elas, Janusz M. Dabrowski
SCIENTIFIC REPORTS
(2019)
Article
Biochemistry & Molecular Biology
Kamil Brzoska, Malgorzata Szczygiel, Agnieszka Drzal, Martyna Sniegocka, Dominika Michalczyk-Wetula, Eva Biela, Martyna Elas, Lucyna Kapka-Skrzypczak, Hanna Lewandowska-Siwkiewicz, Krystyna Urbanska, Marcin Kruszewski
Summary: Gold nanoparticles (AuNPs) were investigated for their potential anticancer effects in tumor growth and metastasis. The results showed that AuNPs did not inhibit tumor growth but caused significant vasodilation in the tumor tissue.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Physical
Kamil Brzoska, Maria Wojewodzka, Malgorzata Szczygiel, Agnieszka Drzal, Martyna Sniegocka, Dominika Michalczyk-Wetula, Eva Biela, Martyna Elas, Malgorzata Kucinska, Hanna Piotrowska-Kempisty, Lucyna Kapka-Skrzypczak, Marek Murias, Krystyna Urbanska, Marcin Kruszewski
Summary: This study analyzed the impact of different silver nanoparticles on the development and metastatic potential of breast cancer using a mouse model. The results showed that citrate-coated silver nanoparticles had a significant effect on reducing tumor volume and lung metastases in mice treated intragastrically. However, no effect on lung metastases was observed after intravenous administration of silver nanoparticles. The findings suggest that the anticancer activity of silver nanoparticles may depend on the activation of the immune system.
Article
Oncology
Anna Markiewicz, Piotr Donizy, Monika Nowak, Mateusz Krzyzinski, Martyna Elas, Przemyslaw M. Plonka, Jolanta Orlowska-Heitzmann, Przemyslaw Biecek, Mai P. Hoang, Bozena Romanowska-Dixon
Summary: The degree of tumour pigmentation is an important prognostic factor for uveal melanoma. Patients with amelanotic tumours have longer survival and delayed tumour spread compared to heavily pigmented tumours. Heavily pigmented tumours also have unfavourable prognostic features. Amelanotic tumours with a lower clinical stage have the best prognosis.
Article
Biochemistry & Molecular Biology
Agnieszka Drza, Anthony Delalande, Gabriela Dziurman, Mylene Fourni, Chantal Pichon, Martyna Elas
Summary: Low tissue oxygenation hinders cancer therapy, but oxygen microbubbles have been found to effectively and safely deliver oxygen to tumors, potentially enhancing the effectiveness of other anticancer therapies.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Agnieszka Drzal, Anthony Delalande, Gabriela Dziurman, Chantal Pichon, Jan Swakon, Martyna Elas
Summary: The inadequate oxygenation in tumors is associated with increased invasiveness of cancer cells and reduced sensitivity to anticancer therapies. Attempts to increase tumor oxygenation have been largely ineffective. The use of phospholipid-coated oxygen microbubbles (OMB) can increase tumor oxygenation, but it also affects tumor vasculature and promotes cancer cell spread.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Chemistry, Physical
Kamil Brzoska, Barbara Sochanowicz, Malgorzata Szczygiel, Agnieszka Drzal, Martyna Sniegocka, Dominika Michalczyk-Wetula, Martyna Elas, Lucyna Kapka-Skrzypczak, Marcin Kruszewski
Summary: The epigenetic effects induced by citrate- and PEG-coated silver nanoparticles (AgNPs) in a 4T1 breast cancer mouse model were analyzed. It was found that both types of AgNPs significantly decreased the level of 5-methylcytosine (5-mC) in tumor tissue, with the effect being more pronounced for citrate-coated AgNPs. PEG-coated AgNPs also caused a significant decrease in DNA methylation after intravenous administration, and both types of AgNPs led to a decrease in histone H3 methylation.
Article
Biochemistry & Molecular Biology
Agnieszka Drzal, Gabriela Dziurman, Pawel Hola, Jakub Lechowski, Anthony Delalande, Jan Swakon, Chantal Pichon, Martyna Elas
Summary: Radiotherapy is a crucial part of cancer treatment, but challenges such as tumor hypoxia and resistance to radiation still exist. Vascular normalization, which aims to improve oxygen supply, has shown promise. This study explores the combination of metformin, an anti-diabetic drug, and oxygen microbubbles to achieve vascular normalization and enhance the effectiveness of radiotherapy. The findings demonstrate that the combination treatment has a more pronounced and sustained effect on normalizing abnormal cancer vasculature compared to individual treatments. It also increases the radiosensitivity of the tumors, although resistance to treatment and recurrence of hypoxia remain as challenges.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
