Journal
RADIATION RESEARCH
Volume 173, Issue 1, Pages 1-9Publisher
RADIATION RESEARCH SOC
DOI: 10.1667/RR1851.1
Keywords
-
Funding
- National Institute of Allergies and Infectious Diseases [AI067733-03]
- National Cancer Institute of Canada [14246]
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [U19AI067733] Funding Source: NIH RePORTER
Ask authors/readers for more resources
Accurate biodosimetry is needed to estimate radiation doses received in vivo from accidental or unwarranted radiation exposures. We investigated the use of DNA repair foci (e.g. gamma-H2AX) at late times after irradiation in vivo as a biodosimeter of initial ionizing radiation dose. Two radiosensitive strains (SCID and BALB/c) and two radioresistant strains (C57BL/6 and C3H/HeJ) were used to quantify gamma-H2AX foci in a skin tissue microarray after doses of 1 to 10 Gy at early and late times after irradiation (1 and 7 days). Using a 3D quantitative immunofluorescence microscopy analysis, we observed a dose response for gamma-H2AX foci for all strains at 30 min, 24 h and 7 days after irradiation. The numbers of residual foci were significantly different between each of the four strains and reflected the relative radiosensitivity in vivo. In comparing gamma-H2AX focus and micronucleus formation after irradiation, we also observed association between the number of micronuclei and number of foci after I and 7 days between radiosensitive and radioresistant strains. We conclude that 3D image analysis of gamma-H2AX in skin can he used to detect relative radiosensitivity based on late residual gamma-H2AX foci. This technique may be a useful biodosimeter to determine dose at times up to I week after accidental or catastrophic radiation exposure in vivo. (C) 2010 by Radiation Research Society
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available