Article
Chemistry, Multidisciplinary
Johannes Gottschalk, Miriam Assmann, Juergen Kuballa, Lothar Elling
Summary: The study focused on enzymatic synthesis of industrial hyaluronic acid, using immobilized cascade enzymes on magnetic beads to increase productivity and facilitate enzyme recovery. Results showed successful production of high-quality HA in repetitive batches.
Article
Pharmacology & Pharmacy
Oliwia Degorska, Daria Szada, Agata Zdarta, Wojciech Smulek, Teofil Jesionowski, Jakub Zdarta
Summary: In this study, lipase immobilized from Aspergillus niger by physical immobilization and mixed physical immobilization and ion exchange was used for kinetic resolution of the ketoprofen racemic mixture. The immobilized lipase showed improved activity and stability, and phosphate buffer at pH 7 was found to be the best solvent.
Review
Polymer Science
Hong Wang, Hongpeng Li, Chee Keong Lee, Noreen Suliani Mat Nanyan, Guan Seng Tay
Summary: This article presents the reaction modes of enzyme-catalyzed polyester synthesis and the mechanism of lipase-catalyzed polymerization. It also discusses the advantages and disadvantages of enzyme-catalyzed polyester synthesis under different solvent systems. Furthermore, it examines the challenges for further industrialization of this method.
Article
Biochemical Research Methods
Julia Seithuemmer, Melda Oeztuerk, Dennis S. Wunschik, Joscha Priessen, Heyko J. Schultz, Michael Dornbusch, Jochen S. Gutmann, Kerstin Hoffmann-Jacobsen
Summary: In this study, an aliphatic-aromatic polyester with two different classes of pendant hydroxyl groups was synthesized enzymatically using lipase catalysis. The addition of hexane diol as a third monomer was required for the polycondensation to occur. The reaction conditions were optimized to incorporate the aromatic diol and enhance the hydroxyl group density. Post-polymerization with glycerol further improved the polymer properties. The resulting material with aromatic building blocks and enhanced polymer chain reactivity has potential applications in coatings and adhesives.
BIOTECHNOLOGY JOURNAL
(2022)
Article
Chemistry, Physical
Helena D. D. Tjornelund, Jesper Brask, John M. M. Woodley, Gunther H. J. Peters
Summary: In this study, an optimized protocol for controlling the initial water activity in organic reaction mixtures with soluble lipase was proposed. The protocol ensures reproducible and consistent enzyme activity measurements. The importance of consistent stirring and volume correction to compensate for solvent evaporation was highlighted. The study also tested the protocol on soluble and immobilized lipase and found similar water activity profiles and the highest enzyme activity in hexane. This optimized protocol expands the possibilities for studying enzyme kinetics.
Article
Chemistry, Multidisciplinary
Meng Sun, Shiqiao Peng, Lei Nie, Yunqing Zou, Li Yang, Laiben Gao, Xiaoqiu Dou, Changli Zhao, Chuanliang Feng
Summary: By transferring chirality from molecular chirality to achiral polymers, a 3D chiral catalytic microenvironment has been constructed, showing significantly enhanced catalytic activity and efficient recyclability, which can improve the catalytic efficiency of lipase.
Article
Biochemistry & Molecular Biology
Dennis Sebastian Wunschik, Andre Lorenz, Kim Nadine Ingenbosch, Jochen Stefan Gutmann, Kerstin Hoffmann-Jacobsen
Summary: A reusable support system for immobilizing lipases is developed using hybrid polymer-inorganic core-shell nanoparticles, with PMMA as the polymer brush. The immobilization system shows enhanced activity and stability compared to the enzyme in solution. The surface structure of the immobilization system significantly affects its reusability.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2022)
Article
Engineering, Chemical
Jian-Hong Fan, Jian Ou, Kewen Tang
Summary: By modifying the immobilized lipase with metal-organic frameworks, the kinetic resolution of mandelic acid enantiomers can be achieved. The optimized conditions and excellent outcomes demonstrate the potential application value of this enzymatic process in kinetic resolution reactions.
CHEMICAL ENGINEERING & TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Vivek C. Badgujar, Kirtikumar C. Badgujar, Pravin M. Yeole, Bhalchandra M. Bhanage
Summary: The study focuses on the synthesis of lab-made carboxymethyl cellulose (CMC) and chitosan (CHI) based co-polymer cross-linked with glutaraldehyde (GLU) for immobilization of Candida rugosa (CRL) lipase (CMC:CHI:GLU:CRL). The immobilized biocatalyst showed significant activity-stability and half-life-time in ultrasonicated medium, enabling excellent conversion of biomass-derived furfuryl derivative. Further optimization of synthetic protocol and recyclability study demonstrated the efficiency of the developed biocatalyst in green and sustainable synthesis.
ENZYME AND MICROBIAL TECHNOLOGY
(2021)
Article
Microbiology
Dennis Sander, Yanfei Yu, Premankur Sukul, Sina Schaekermann, Julia E. Bandow, Trinetra Mukherjee, Subhra Kanti Mukhopadhyay, Lars Leichert
Summary: Lipolytic enzymes are important biocatalysts produced by various organisms and widely used in industrial applications. A new metaproteomics approach was used to identify a novel thermo- and solvent-stable lipolytic enzyme, DS-007, which exhibited high hydrolytic activity with substrates of shorter chain length.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Fumiya Wayama, Noriyuki Hatsugai, Yasuaki Okumura
Summary: This study reports an effective method using bipyridines as electron transfer mediators for NADPH production. The results showed that bipyridines with a reduction potential near -1.0 V can effectively transfer electrons to NADP(+), resulting in NADPH production. Additionally, it was found that bipyridines with small substituents at the 1- and 1'-positions can efficiently produce NADPH, while bipyridines with methyl substituents can cause dehydrogenation reaction of NADPH, making them unsuitable as electron mediators.
Review
Chemistry, Physical
Francisco T. T. Cavalcante, Antonio L. G. Cavalcante, Isamayra G. de Sousa, Francisco S. Neto, Jose C. S. dos Santos
Summary: The market for industrial enzymes is growing rapidly, with lipases being the most widely used industrial biocatalysts. However, challenges such as low stability and complex solutions require new materials and technologies to improve their performance.
Review
Microbiology
Sajid Ali, Sumera Afzal Khan, Muhammad Hamayun, In-Jung Lee
Summary: This article provides a comprehensive overview of the application and extraction techniques of microbial lipases in various industries, as well as their role in biosensors, biodiesel production, tea processing, bioremediation, and racemization.
Article
Chemistry, Multidisciplinary
Ludwig L. Bengel, Benjamin Aberle, Alexander-N. Egler-Kemmerer, Samuel Kienzle, Bernhard Hauer, Stephan C. Hammer
Summary: Selective alkylation of pyrazoles using a catalyst-controlled enzymatic system allows for the efficient synthesis of important molecules with unprecedented regioselectivity. The use of a computational enzyme-library design tool enabled the conversion of a promiscuous enzyme into a small enzyme family of pyrazole-alkylating enzymes in one round of mutagenesis and screening. This enzymatic system demonstrates high selectivity and achieves different alkylations of pyrazoles with remarkable regiodivergence.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biochemistry & Molecular Biology
Julio Cesar Soares Sales, Aline Machado de Castro, Bernardo Dias Ribeiro, Maria Alice Zarur Coelho
Summary: This study evaluated the depolymerization of PET by Y. lipolytica using two different strategies. The results indicate that this microorganism is capable of effectively degrading PET, and using YT medium in submerged cultures led to a higher conversion rate.
Article
Engineering, Chemical
Ivone Sampaio Pereira Campisano, Erika de Queiros Eugenio, Claudia de Oliveira Veloso, Marcos Lopes Dias, Aline Machado de Castro, Marta Antunes Pereira Langone
Summary: Aliphatic polyesters, widely used in biomedical and environmental applications, have shown increased utilization due to environmental concerns. The use of lipases as catalysts for polyester synthesis has been explored as an environmentally friendly alternative. This study found that monomers with longer alkylene chains exhibited higher reactivity in polycondensation reactions, with polyesters formed with 1,6-hexanediol and azelaic or sebacic acids showing higher molecular weights. Additionally, the thermal stability of the resulting polyesters was demonstrated up to 380 degrees C, indicating their potential for high-temperature applications.
BRAZILIAN JOURNAL OF CHEMICAL ENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Erika de Queiros Eugenio, Ivone Sampaio Pereira Campisano, Aline Machado de Castro, Maria Alice Zarur Coelho, Marta Antunes Pereira Langone
Summary: The study evaluated the enzymatic hydrolysis of PET waste using cutinase from Humicola insolens, and found that the increase in PC-PET specific area significantly impacts the reaction rate. The optimal enzyme concentration for reaction rate was determined to be 1.68 mgprotein mL-1, and the enzyme exhibited a half-life of 110 hours at 70℃ and pH 7.0.
JOURNAL OF BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Gabriella Neves Ricarte, Marcos Lopes Dias, Lys Syrelli, Marta Antunes Pereira Langone, Aline Machado de Castro, Maria Alice Zarur Coelho, Bernardo Dias Ribeiro
Summary: This study investigated a two-step PET depolymerization approach involving chemical glycolysis and enzymatic hydrolysis, achieving a high overall yield of 57% TPA from PET. The integrated method proved to be effective in converting PET into TPA within a short processing timeframe.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Applied
Sabrini N. S. Avila, Ana Cristina A. Collaco, Jaqueline Greco-Duarte, Erika C. G. Aguieiras, Priscila R. S. Ambrosio, Aline M. de Castro, Melissa L. E. Gutarra, Elisa D. C. Cavalcanti, Denise M. G. Freire
Summary: A new green integrated process for biodiesel ester production was developed, using low-cost biocatalysts and a low-value oil for enzymatic transesterification. This method can reduce production costs and optimize raw material utilization.
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
(2021)
Article
Biochemistry & Molecular Biology
Daniel Tinoco, Aline M. de Castro, Lucy Seldin, Denise M. G. Freire
Summary: This study successfully increased the production of 2,3-butanediol by Paenibacillus polymyxa PM 3605 using biodiesel-derived glycerol and sugarcane molasses. The highest titer of 2,3-BDO produced from crude glycerol with the use of a secondary substrate has been achieved, showing promising potential for further improvement in the future.
PROCESS BIOCHEMISTRY
(2021)
Review
Biotechnology & Applied Microbiology
Priscila Esteves Faria, Aline M. Castro, Denise Maria Guimaraes Freire, Rafael D. Mesquita
Summary: This article elucidates the details of isomer production of 2,3-butanediol and acetoin, non-oxidative metabolism products of microorganisms, through bioinformatics analysis and compares the stereo-triads of other enzymes. These results are of great importance for understanding the biosynthesis and industrial production of BD/AC.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Aline M. Castro, Adriano Carniel, Sonia M. Menezes, Luiz S. Chinelatto Junior, Hercilio Honorato, Aline P. Lima, Erica G. Chaves
Summary: This study demonstrates the enzymatic depolymerization of PET from mooring lines using cutinase enzyme, adding valuable insight for circular economy of plastic products. The results showed that new mooring lines had lower molecular weight compared to used ones, and terephthalic acid concentration increased significantly during depolymerization reactions.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2022)
Article
Engineering, Environmental
Erika de Queiros Eugenio, Ivone Sampaio Pereira Campisano, Aline Machado de Castro, Maria Alice Zarur Coelho, Marta Antunes Pereira Langone
Summary: The study evaluated a kinetic model for the enzymatic hydrolysis of PC-PET using HiC as a catalyst, showing that HiC is not inhibited by major PET hydrolysis products. The experimental data fit well with the kinetic model, validating its use in describing the enzymatic hydrolysis process. The study also provided reliable parameters for the enzymatic hydrolysis, demonstrating the potential of HiC as a biocatalyst for PC-PET degradation in a circular economy.
JOURNAL OF POLYMERS AND THE ENVIRONMENT
(2022)
Article
Engineering, Chemical
Felipe Valle do Nascimento, Ailton Cesar Lemes, Aline Machado de Castro, Argimiro Resende Secchi, Maria Alice Zarur Coelho
Summary: This article presents a temporal evolution perspective on the main aspects of lipase production through solid-state fermentation (SSF) using Yarrowia lipolytica. The advantages of this approach include high volumetric productivity, targeting concentrated compounds, and reducing wastewater generation.
Article
Biochemistry & Molecular Biology
Julio Cesar Soares Sales, Aline Machado de Castro, Bernardo Dias Ribeiro, Maria Alice Zarur Coelho
Summary: This study evaluated the depolymerization of PET by Y. lipolytica using two different strategies. The results indicate that this microorganism is capable of effectively degrading PET, and using YT medium in submerged cultures led to a higher conversion rate.
Article
Biochemistry & Molecular Biology
Suchintan Mondal, Bhavna Alke, Aline Machado de Castro, Paloma Ortiz-Albo, Usman Taqui Syed, Joao G. Crespo, Carla Brazinha
Summary: Membrane-based gas separation is a promising unit operation for a low-carbon economy. This study proposes a method to immobilize enzymes in emulsions and use them in the pores of a membrane, creating emulsion-based supported liquid membranes. The research focuses on using these membranes for biogas purification and successfully demonstrates the enhancement of CO2 capture through emulsion-based supported liquid membranes.
Article
Biochemical Research Methods
Adriano Carniel, Ariane Gaspar Santos, Luiz Silvino Chinelatto Jr, Aline M. Castro, Maria Alice Zarur Coelho
Summary: Recently, biological recycling of PET waste has been extensively studied, and ethylene glycol (EG) is one of the main building blocks recovered. Wild-type Yarrowia lipolytica IMUFRJ 50682 can be used as a biocatalyst to biodepolymerize PET. This study reports its ability to oxidize EG into glycolic acid (GA), a higher value-added chemical with various industrial applications.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Polymer Science
Lusiane Malafatti-Picca, Elaine Cristina Bucioli, Michel Ricardo de Barros Chaves, Aline Machado de Castro, Erika Valoni, Valeria Maia de Oliveira, Anita Jocelyne Marsaioli, Jose Silvio Govone, Dejanira de Franceschi de Angelis, Michel Brienzo, Derlene Attili-Angelis
Summary: Approximately 400 billion PET bottles are produced annually in the world, with 8 to 9 million tons discarded in oceans. PET recycling using microbial hydrolysis shows promise for treating harmful polymeric waste. The study identified four fungal strains suitable for converting PET nanoparticles into terephthalic acid, and confirmed biodegradation through various analyses.
Article
Biotechnology & Applied Microbiology
Rodrigo Brackmann, Claudia de Oliveira Veloso, Aline Machado de Castro, Marta Antunes Pereira Langone
Summary: This study investigated the degradation of post-consumer PET using five commercial hydrolase enzymes. Humicola insolens cutinase (HiC) was found to be the most active enzyme. The concentration and ratio of hydrolysis products varied significantly with reaction temperature. The enzymatic PET hydrolysis by HiC was sensitive to enzyme concentration, with a maximum achieved at 40-50 vol% of HiC. These findings contribute to optimizing enzyme-based PET recycling technologies.
Article
Polymer Science
Ana P. Arcanjo, Denisson O. Liborio, Santiago Arias, Florival R. Carvalho, Josivan P. Silva, Bernardo D. Ribeiro, Marcos L. Dias, Aline M. Castro, Roger Frety, Celmy M. B. M. Barbosa, Jose Geraldo A. Pacheco
Summary: In this study, the chemical recycling of PET residues was carried out using Mg-Fe and Mg-Al oxide catalysts derived from layered double hydroxides, with glycolysis using ethylene glycol as the catalyst. The catalysts prepared using the low supersaturation method showed higher PET conversion compared to the high supersaturation method. The Mg-Fe catalysts exhibited higher PET conversion than the Mg-Al catalysts, and could be reused multiple times with high conversion rates.
