Journal
QUARTERLY REVIEWS OF BIOPHYSICS
Volume 42, Issue 4, Pages 247-300Publisher
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0033583510000016
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Funding
- Japan Science and Technology Corporation (JST)
- Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT)
- Japan Society for the Promotion of Science (JSPS)
- Volkswagen Foundation
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Optimal stereospecific and regiospecific labeling of proteins with stable isotopes enhances the nuclear magnetic resonance (NMR) method for the determination of the three-dimensional protein structures in solution. Stereo-array isotope labeling (SAIL) offers sharpened lines, spectral simplification without loss of information and the ability to rapidly collect and automatically evaluate the structural restraints required to solve a high-quality solution structure for proteins up to twice as large as before. This review gives an overview of stable isotope labeling methods for NMR spectroscopy with proteins and provides an in-depth treatment of the SAIL technology.
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