Review
Medicine, Research & Experimental
Zhichao Wang, Wenjie Xie, Hongzai Guan
Summary: MicroRNAs are small non-coding RNAs that interact with their target mRNAs to degrade them or inhibit translation. Dysregulation of miRNAs is associated with various human cancers and plays a role in the development of cancer pathology. MiR-328 has been implicated in multiple human cancers and considered a key regulator. It is involved in biological processes such as cell proliferation, apoptosis, invasion, migration, and epithelial-mesenchymal transition. This review combines basic and clinical studies to demonstrate that miR-328 promotes tumorigenesis and metastasis in human cancer and discusses its diagnostic, prognostic, and therapeutic value.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Cell Biology
Xiuhua Su, Guangxin Ma, Xiaoran Bai, Juan Zhang, Mingying Li, Fan Zhang, Tao Sun, Daoxin Ma, Fei Lu, Chunyan Ji
Summary: The study showed that FLVCR2 is significantly upregulated in AML patients and its upregulation predicts poor overall survival. FLVCR2 may have a crucial effect on AML progression through the immune response.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Anupama Ninawe, Sameer Ahmad Guru, Prasant Yadav, Mirza Masroor, Amit Samadhiya, Namrata Bhutani, Naresh Gupta, Richa Gupta, Alpana Saxena
Summary: The study reveals alterations in the expression levels of miR-486-5p in CML patients, suggesting its potential as a biomarker for diagnosis and prognosis. The findings indicate a critical role of miR-486-5p in the treatment and prognosis of CML patients.
Article
Oncology
Yaqi Cheng, Xiaoran Wang, Peiyan Qi, Chengxiu Liu, Shoubi Wang, Qi Wan, Yurun Liu, Yaru Su, Lin Jin, Ying Liu, Chaoyang Li, Xuan Sang, Liu Yang, Chang Liu, Hucheng Duan, Zhichong Wang
Summary: This study aimed to build a ceRNA network related to AML TME using RNA expression data from the TCGA database. The research identified a hub prognostic ceRNA network composed of specific mRNAs, miRNAs, and lncRNAs using various algorithms and survival analyses. The findings suggested a correlation between immune cell infiltration in TME and AML prognosis, highlighting potential therapeutic targets.
FRONTIERS IN ONCOLOGY
(2021)
Article
Oncology
Juan Rodrigo Patino-Mercau, Carlos Balinas-Gavira, Alvaro Andrades, Maria S. Benitez-Cantos, Ana Ercegovic Rot, Maria Isabel Rodriguez, Juan Carlos Alvarez-Perez, Marta Cuadros, Pedro P. Medina
Summary: Recent sequencing studies have shown that SWI/SNF complexes are frequently altered in solid tumors, but their role in acute myeloid leukemia (AML) is not well understood. This study focuses on the role of the BCL7A subunit in AML, which is recurrently mutated in lymphomas. The findings suggest that BCL7A is silenced by promoter hypermethylation in AML and its restoration suppresses tumor growth in AML cell lines and xenograft models.
BIOMARKER RESEARCH
(2023)
Article
Genetics & Heredity
Chikashi Minemura, Shunichi Asai, Ayaka Koma, Naoko Kikkawa, Mayuko Kato, Atsushi Kasamatsu, Katsuhiro Uzawa, Toyoyuki Hanazawa, Naohiko Seki
Summary: Analysis of miRNA expression in head and neck squamous cell carcinoma (HNSCC) revealed downregulation of the miR-30 family, especially miR-30e-5p and miR-30c-1-3p, which are associated with shorter patient survival. Further investigation showed that miR-30e-5p suppresses HNSCC cell migration and controls multiple oncogenic genes, including FOXD1. Knockdown of FOXD1 inhibited malignant phenotypes of HNSCC cells. These findings provide potential prognostic markers and therapeutic targets for HNSCC.
Article
Immunology
Paola Del Bianco, Laura Pinton, Sara Magri, Stefania Cane, Elena Masetto, Daniela Basso, Marta Padovan, Francesco Volpin, Domenico d'Avella, Giuseppe Lombardi, Vittorina Zagonel, Vincenzo Bronte, Alessandro Della Puppa, Susanna Mandruzzato
Summary: This study highlights the role of systemic immune suppression carried out by myeloid cells in gliomas and identifies biomarkers associated with the immune landscape, diagnosis, and outcome of glioblastoma patients.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Can Yue, Siting Xie, Jiaying Zhong, Haijun Zhao, Zhijuan Lin, Li Zhang, Bing Xu, Yiming Luo
Summary: The study found that SCAMP2/4/5 may serve as potential prognostic markers for AML, and SCAMP2 and SCAMP5 individually or in combination may be utilized as diagnostic markers for AML.
SCIENTIFIC REPORTS
(2021)
Review
Immunology
Rong Fan, Nathan De Beule, Anke Maes, Elke De Bruyne, Eline Menu, Karin Vanderkerken, Ken Maes, Karine Breckpot, Kim De Veirman
Summary: MDSC play a key role in the immunosuppressive microenvironment of hematological cancers, associated with tumor cell survival and drug resistance. Despite intensive studies on the phenotype, function, and prognostic value of MDSC in hematological cancers, therapeutic targeting of this cell population remains challenging.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Neurosciences
Iddo Magen, Nancy Sarah Yacovzada, Eran Yanowski, Anna Coenen-Stass, Julian Grosskreutz, Ching-Hua Lu, Linda Greensmith, Andrea Malaspina, Pietro Fratta, Eran Hornstein
Summary: The study identified miRNA-based biomarkers in the blood of ALS patients, with miR-181 levels alone or in combination with established protein biomarkers predicting disease severity and prognosis. This approach may enhance the power of clinical trials for ALS and improve patient stratification accuracy.
NATURE NEUROSCIENCE
(2021)
Editorial Material
Hematology
Ian J. Majewski
Summary: The study investigates how loss of the tumor suppressor gene Cux1 modulates DNA repair activity in the hematopoietic compartment and contributes to the pathogenesis of therapy-related myeloid neoplasms.
Article
Oncology
Hong Zhou, Pengfei Shi, Xiaofeng Jia, Qianfu Xue
Summary: Acute myeloid leukemia (AML) is a highly heterogeneous disease with a high mortality rate. Recent studies have indicated that long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) may serve as useful biomarkers in AML. LINC01018 inhibits AML cell growth by modulating miR-499a-5p and PDCD4, providing a theoretical basis for AML treatment.
Article
Medical Laboratory Technology
Liang Guo, Ru Kou, Yanping Song, Guang Li, Xueyou Jia, Zhenzhen Li, Yunjie Zhang
Summary: This study found that the expression of hsa_circ_0079480 is upregulated in the serum of AML patients and is closely related to clinical characteristics and prognosis. Patients with poorer prognosis had higher levels of hsa_circ_0079480. Serum levels of hsa_circ_0079480 may serve as a potential biomarker for clinical diagnosis and prognosis evaluation in AML.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2022)
Article
Oncology
Shenrui Bai, Huizhong Wang, Ruonan Shao, Bibo Fu, Shujing Lu, Jingzi Wang, Yue Lu, Hua Wang
Summary: This study examined the correlation between serum lipid levels and clinical outcomes in acute myeloid leukemia (AML) by creating a predictive risk classification model. The results indicated that serum lipid levels are associated with the prognosis of AML, and a five-factor model was established to categorize patients into high- and low-risk groups.
FRONTIERS IN ONCOLOGY
(2023)
Article
Medicine, Research & Experimental
Jinye Xie, Kang Chen, Hui Han, Qian Dong, Weijia Wang
Summary: This study aimed to establish TP53 mutation-based prognostic risk signatures for patients with acute myeloid leukemia (AML). Through transcriptome analysis, the researchers constructed mRNA and lncRNA-based risk signatures to predict overall survival. These signatures showed potential value in stratifying high-risk and low-risk patients and could serve as a tool for AML prognosis prediction and management.
CURRENT RESEARCH IN TRANSLATIONAL MEDICINE
(2022)
Review
Genetics & Heredity
Ryan L. Kan, Jianjun Chen, Tamer Sallam
Summary: Epigenetic modifications play a crucial role in gene expression regulation through modifications on genomic DNA, histones, and more recently, mRNA. These modifications, especially the common N6-methyladenosine (m6A) methylation, not only influence RNA stability and translation efficiency, but also interact with histone/DNA epigenetic mechanisms to specify transcriptional outputs and recruit chromatin modifiers. This review focuses on dissecting the feedback circuits orchestrated by m6A, which regulate histone modifications and the activity of regulatory RNAs, such as long noncoding RNA and chromosome-associated regulatory RNA. The evidence suggests that m6A acts as a versatile checkpoint linking different layers of gene regulation.
TRENDS IN GENETICS
(2022)
Article
Cell Biology
Jiong Li, Ke Chen, Xin Dong, Yating Xu, Qi Sun, Honghong Wang, Zhen Chen, Cong Liu, Rong Liu, Zhe Yang, Xiangfei Mei, Rongyu Zhang, Liuping Chang, Zongwen Tian, Jianjun Chen, Kaiwei Liang, Chunjiang He, Mengcheng Luo
Summary: YTHDF1 recruits AGO2 through the YTH domain and degrades target mRNAs by promoting P-body formation through LLPS. Deletion of YTHDF1 causes P-bodies to transition from liquid droplets to gel/solid droplets, forming AGO2/RNA patches and delaying mRNA degradation. These findings indicate a previously unrecognized crosstalk between YTHDF1 and AGO2 in post-transcriptional mRNA regulation.
CELL PROLIFERATION
(2022)
Article
Cell Biology
Rui Su, Lei Dong, Yangchan Li, Min Gao, P. Cody He, Wei Liu, Jiangbo Wei, Zhicong Zhao, Lei Gao, Li Han, Xiaolan Deng, Chenying Li, Emily Prince, Brandon Tan, Ying Qing, Xi Qin, Chao Shen, Meilin Xue, Keren Zhou, Zhenhua Chen, Jianhuang Xue, Wei Li, Hanjun Qin, Xiwei Wu, Miao Sun, Yunsun Nam, Chun-Wei Chen, Wendong Huang, David Horne, Steven T. Rosen, Chuan He, Jianjun Chen
Summary: METTL16 has dual functions as an m(6)A writer in the nucleus and a translation facilitator in the cytosol, promoting translation through direct interactions with ribosomal RNAs and eukaryotic initiation factors.
NATURE CELL BIOLOGY
(2022)
Review
Genetics & Heredity
Chao Shen, Kitty Wang, Xiaolan Deng, Jianjun Chen
Summary: This article reviews the recent advances in N-6-methyladenine (6mA) research in eukaryotes, especially mammals. While 6mA has been shown to be an important epigenetic mark in eukaryotes and plays a regulatory role in DNA transcription, transposon activation, stress response, and other biological processes, there are discrepancies in the current literature. The abundance/distribution of 6mA, its potential role in regulating gene expression, identified regulators, and pathological roles in human diseases, particularly cancer, are discussed. The limitations faced by the field and future perspectives in 6mA research are also addressed.
TRENDS IN GENETICS
(2022)
Article
Cell Biology
Huanhuan Zhao, Jun Lu, Tong Yan, Fei Han, Jie Sun, Xiaolin Yin, Liting Chen, Chao Shen, Mark Wunderlich, Weina Yun, Lingling Yang, Liyun Chen, Dan Su, Stefan K. Bohlander, Fudi Wang, James C. Mulloy, Chong Li, Jianjun Chen, He Huang, Xi Jiang
Summary: Researchers have discovered that the opioid receptor agonist OPA1 can suppress the viability of AML cells, especially in TET2-mutated and chemotherapy-resistant cases.
Article
Cell & Tissue Engineering
Li Han, Lei Dong, Keith Leung, Zhicong Zhao, Yangchan Li, Lei Gao, Zhenhua Chen, Jianhuang Xue, Ying Qing, Wei Li, Sheela Pangeni Pokharel, Min Gao, Meiling Chen, Chao Shen, Brandon Tan, Andrew Small, Kitty Wang, Zheng Zhang, Xi Qin, Lu Yang, Mark Wunderlich, Bin Zhang, James C. Mulloy, Guido Marcucci, Chun-Wei Chen, Minjie Wei, Rui Su, Jianjun Chen, Xiaolan Deng
Summary: This study reveals that N6-methyladenosine (m6A), the most prevalent internal modification in mammalian mRNAs, is involved in many pathological processes. METTL16, a recently identified m6A methyltransferase, plays a crucial role in the survival of acute myeloid leukemia (AML) cells.
Article
Immunology
Shoubao Ma, Baofa Sun, Songqi Duan, Jingjing Han, Tasha Barr, Jianying Zhang, Marc B. Bissonnette, Marcin Kortylewski, Chuan He, Jianjun Chen, Michael A. Caligiuri, Jianhua Yu
Summary: Caligiuri and colleagues demonstrate that the m(6)A reader YTHDF2 controls the inflammatory activation and antitumor function of tumor-associated macrophages by regulating the stability of Stat1 mRNA. They show that YTHDF2 deficiency in TAMs reprograms them towards an antitumoral phenotype, enhancing their antigen cross-presentation ability and CD8(+) T cell-mediated antitumor immunity. Moreover, targeting YTHDF2 in TAMs using a Toll-like receptor 9 agonist-conjugated small interfering RNA restrains tumor growth and improves the efficacy of PD-L1 antibody therapy. These findings suggest that YTHDF2 inhibition is a promising strategy to enhance cancer immunotherapy.
Article
Biotechnology & Applied Microbiology
Bin Li, Shurong Liu, Wujian Zheng, Anrui Liu, Peng Yu, Di Wu, Jie Zhou, Ping Zhang, Chang Liu, Qiao Lin, Jiayi Ye, Simeng He, Qiaojuan Huang, Hui Zhou, Jianjun Chen, Lianghu Qu, Jianhua Yang
Summary: A new method called RIP-PEN-seq was developed to identify the full-length sequences of RNAs bound by the K-turn binding protein 15.5K. This study discovered a previously unknown class of RNAs with backward K-turn motifs (bktRNAs) in humans and mice. These bktRNAs share two consensus sequence motifs at their fixed terminal position and have complex folding properties, expression and evolution patterns. The research also revealed that bktRNA1 guides the methyltransferase fibrillarin to install RNA methylation of U12 small nuclear RNA in humans.
NATURE BIOTECHNOLOGY
(2023)
Review
Oncology
Xiaolan Deng, Ying Qing, David Horne, Huilin Huang, Jianjun Chen
Summary: m(6)A modification is prevalent in eukaryotic mRNA and dysregulation of this modification is associated with various cancer types. This review provides an overview of the mechanisms, pathological effects, and potential therapeutic targets of m(6)A modification in cancer.
NATURE REVIEWS CLINICAL ONCOLOGY
(2023)
Article
Cell Biology
Zhenhua Chen, Keren Zhou, Jianhuang Xue, Andrew Small, Gang Xiao, Le Xuan Truong Nguyen, Zheng Zhang, Emily Prince, Hengyou Weng, Huilin Huang, Zhicong Zhao, Ying Qing, Chao Shen, Wei Li, Li Han, Brandon Tan, Rui Su, Hanjun Qin, Yangchan Li, Dong Wu, Zhaohui Gu, Vu N. Ngo, Xin He, Jianfel Chao, Keith Leung, Kitty Wang, Lei Dong, Xi Qin, Zhenming Cal, Yue Sheng, Yu Chen, Xiwei Wu, Bin Zhang, Yanhong Shi, Guido Marcucci, Zhijian Qian, Mingjiang Xu, Markus Muschen, Jianjun Chen, Xiaolan Deng
Summary: This study reveals that overexpression of TET1 protein plays a crucial oncogenic role in B-ALL independent of its catalytic activity. Targeting the TET1 signaling pathway can effectively decrease B-ALL cell viability and inhibit progression, providing a potential therapeutic strategy for refractory/relapsed B-ALL.
SCIENCE TRANSLATIONAL MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Yujiao Liu, Dan Yang, Tao Liu, Jianjun Chen, Jianhua Yu, Ping Yi
Summary: N6-methyladenosine (m6A) RNA methylation is the most common form of mRNA modification in eukaryotes, playing a crucial role in biological and biomedical research. This dynamic and reversible modification affects the fate of RNA molecules and is involved in various important biological processes. Dysregulation of m6A modification is linked to diseases, especially cancer. This review provides an overview of post-transcriptional and transcriptional regulatory mechanisms involving m6A modification and discusses its implications in disease development, as well as potential targets for therapeutics and diagnostics.
TRENDS IN MOLECULAR MEDICINE
(2023)
Article
Oncology
Thuy Phan, Vu H. Nguyen, Rui Su, Yangchan Li, Ying Qing, Hanjun Qin, Hyejin Cho, Lei Jiang, Xiwei Wu, Jianjun Chen, Marwan Fakih, Don J. Diamond, Ajay Goel, Laleh G. Melstrom
Summary: This study evaluated the role of Fat mass and obesity-associated protein (FTO) in colorectal cancer (CRC) tumorigenesis. Results showed that the FTO inhibitor, CS1, suppressed CRC cell proliferation and induced apoptosis. Additionally, lentivirus knockdown of FTO also showed similar effects. These findings suggest that FTO may be a potential therapeutic target for CRC.
FRONTIERS IN ONCOLOGY
(2023)
Article
Oncology
Yiming Wu, Meiling Jin, Mike Fernandez, Kevyn L. Hart, Aijun Liao, Xinzhou Ge, Stacey M. Fernandes, Tinisha McDonald, Zhenhua Chen, Daniel Roth, Lucy Y. Ghoda, Guido Marcucci, Markus Kalkum, Raju K. Pillai, Alexey V. Danilov, Jingyi Jessica Li, Jianjun Chen, Jennifer R. Brown, Steven T. Rosen, Tanya Siddiqi, Lili Wang
Summary: RNA splicing dysregulation is a critical factor in the onset and progression of cancers, including chronic lymphocytic leukemia (CLL). Through transcriptomic and proteomic analysis, it has been found that proteins involved in RNA splicing are upregulated in CLL cells, leading to splicing dysregulation. The expression of splicing factors is highly correlated with the abundance of METTL3, an RNA methyltransferase that controls splicing factor protein expression through m6A modification-mediated ribosome recycling and decoding. The findings highlight the significance of METTL3-mediated m6A modification as a potential therapeutic target in aggressive CLL.
BLOOD CANCER DISCOVERY
(2023)
Editorial Material
Biochemistry & Molecular Biology
Chao Shen, Kitty Wang, Wei Li, Alvaro Serrano, Kelly Powers, Chengwan Zhang, Jianjun Chen, Miao Sun
