Journal
BIOMEDICAL OPTICS EXPRESS
Volume 6, Issue 3, Pages 849-858Publisher
OPTICAL SOC AMER
DOI: 10.1364/BOE.6.000849
Keywords
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Funding
- National Science Foundation (NSF) CAREER Award [CBET-1151154]
- National Aeronautics and Space Administration (NASA) Early Career Faculty Grant [NNX12AQ44G]
- Gulf of Mexico Research Initiative [GoMRI-030]
- Cullen College of Engineering
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We report a novel reagent-and separation-free method for urine creatinine concentration measurement using stamping surface enhanced Raman scattering (S-SERS) technique with nanoporous gold disk (NPGD) plasmonic substrates, a label-free, multiplexed molecular sensing and imaging technique recently developed by us. The performance of this new technology is evaluated by the detection and quantification of creatinine spiked in three different liquids: creatinine in water, mixture of creatinine and urea in water, and creatinine in artificial urine within physiologically relevant concentration ranges. Moreover, the potential application of our method is demonstrated by creatinine concentration measurements in urine samples collected from a mouse model of nephritis. The limit of detection of creatinine was 13.2 nM (0.15 mu g/dl) and 0.68 mg/dl in water and urine, respectively. Our method would provide an alternative tool for rapid, cost-effective, and reliable urine analysis for non-invasive diagnosis and monitoring of renal function. (C) 2015 Optical Society of America.
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