Journal
BIOMEDICAL OPTICS EXPRESS
Volume 6, Issue 11, Pages 4493-4500Publisher
OPTICAL SOC AMER
DOI: 10.1364/BOE.6.004493
Keywords
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Funding
- Tianjin Municipal Science and Technology Commission [14JCQNJC01800, 08ZCDFGX09400]
- National Natural Science Fundation of China [61201106, 31470951, 81172837, 61307094]
- Tianjin Special Scientific Grant [14JCPJC00529]
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Here, Raman microspectroscopy was employed to assess replicative senescence of mesenchymal stem cells (MSC). A regular spectral change related to the cell senescence was found in the ratio of two peaks at 1157 cm(-1) and 1174 cm(-1), which are assigned to C-C, C-N stretching vibrations in proteins and C-H bending vibrations in tyrosine and phenylalanine, respectively. With the cell aging, the ratio I-1157 / I-1174 exhibited a monotonic decline and showed small standard deviations, so that it can statistically distinguish between cells having slight changes in terms of aging. We propose that I-1157 / I-1174 can act as a characteristic spectral signature for label-free assessment of MSC senescence. (C) 2015 Optical Society of America
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