Article
Chemistry, Multidisciplinary
Bong Kyun Kim, Sang-A Lee, Minju Park, Eui Ju Jeon, Mi Jung Kim, Jung Min Kim, Heesuk Kim, Seungwon Jung, Sang Kyung Kim
Summary: This article introduces a photonic PCR platform using hydrogel microparticles, which utilizes photothermal nanomaterials as heating elements for rapid and portable POCT. The researchers successfully demonstrate the ability to perform multiplex assays by loading multiple encoded pPIN microparticles in a single reaction.
Article
Multidisciplinary Sciences
Xinyu Dou, Jinpyo Hong, Philippe Ciais, Frederic Chevallier, Feifan Yan, Ying Yu, Yifan Hu, Da Huo, Yun Sun, Yilong Wang, Steven. J. J. Davis, Monica Crippa, Greet Janssens-Maenhout, Diego Guizzardi, Efisio Solazzo, Xiaojuan Lin, Xuanren Song, Biqing Zhu, Duo Cui, Piyu Ke, Hengqi Wang, Wenwen Zhou, Xia Huang, Zhu Deng, Zhu Liu
Summary: This study presents a near-real-time global gridded daily CO2 emissions dataset (GRACED) for 2021. GRACED provides detailed information on CO2 emissions at a high spatial and temporal resolution from various sectors such as industry, power, transportation, and aviation. The dataset is based on reliable national emissions estimates, spatial activity data, and satellite observations, allowing policymakers to monitor and adjust energy and climate policies effectively.
Article
Multidisciplinary Sciences
Walid Hossain, Mohabbat Hossain, Khalid Arafath, Subarna Sayed Ety, Mahade Hasan Shetu, Mazbahul Kabir, Farjana Akther Noor, Kalissar Mannoor
Summary: The study aimed to reduce the run time of multiplex RT-PCR for detecting SARS CoV-2 N1 and N2 sequences and human RNase P (RP) sequence. By optimizing the Fast PCR protocol and comparing it with conventional PCR, the researchers found that Fast PCR significantly shortened the run time while yielding comparable results to conventional PCR.
Article
Chemistry, Analytical
Yuwei Qiu, Kunlun Jiang, Jingrui Wu, Yung-Kang Peng, Jeong-Wook Oh, Jung- Hoon Lee
Summary: A plasmonic photothermal quantitative immuno-PCR (PPT-qiPCR) assay is developed for biomarker detection using multifunctional plasmonic immunomagnetic nanoparticles (PIMNs). This assay has real-time and end-point dual readout and is simple, time-saving, and inexpensive compared to conventional iPCR platforms. It can quickly discriminate target proteins and improve assay sensitivity with in-situ end-point fluorescence or colorimetric detection using only the human eye.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Multidisciplinary
Jing Tu, Yi Qiao, Yuhan Luo, Naiyun Long, Zuhong Lu
Summary: DNA quantification is crucial in life sciences research, and real-time MDA technology can provide template amount information during whole-genome amplification with a very low detection limit, offering a promising tool to quantify entire DNA without sacrificing sample completeness or introducing redundant steps.
Article
Chemistry, Multidisciplinary
Byoung-Hoon Kang, Youngseop Lee, Eun-Sil Yu, Hamin Na, Minhee Kang, Hee Jae Huh, Ki-Hun Jeong
Summary: The rapid and accurate molecular diagnostics are crucial in the face of pandemic diseases, and the nanoplasmonic PCR provides a new solution for rapid and quantitative molecular diagnostics at point-of-care level.
Article
Chemistry, Analytical
Liang Gong, Siyue Yao, Yidong He, Chengliang Liu
Summary: A new qPCR data analysis method called AERKM is proposed to achieve more accurate results by introducing amplification efficiency (AE) for error correction. It has been verified to have higher precision and stability in 5-point 10-fold gradient qPCR tests of 63 genes.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Byoung-Hoon Kang, Kyung-Won Jang, Eun-Sil Yu, Hamin Na, Yun-Jae Lee, Woong-Yeol Ko, NamHo Bae, Donggee Rho, Ki -Hun Jeong
Summary: This study presents a rapid and compact real-time RT-PCR system for decentralized molecular diagnostics. It utilizes plasmonic nucleic acid amplification and quantification, and features an ultrafast plasmonic thermocycler, a disposable plastic-on-metal cartridge, and a compact fluorescence microscope. The system successfully enables 10-minute diagnosis of COVID-19 with high amplification efficiency, classification accuracy, and total percent agreement for clinical diagnostic tests.
Article
Chemistry, Multidisciplinary
Byoung-Hoon Kang, Kyung-Won Jang, Eun-Sil Yu, Hamin Na, Yun-Jae Lee, Woong-Yeol Ko, NamHo Bae, Donggee Rho, Ki-Hun Jeong
Summary: This study presents an ultrafast plasmonic nucleic acid amplification and real-time quantification system for decentralized molecular diagnostics. The system features ultrafast photothermal cycling, rapid heat transfer, and efficient PCR quantification, along with high-contrast fluorescence microscopic imaging. It enables decentralized point-of-care molecular diagnostic testing in primary care and developing countries.
Article
Chemistry, Analytical
Kazuyuki Nobusawa, Huan-Wen Han, Fumie Takei, Ting-Chieh Chu, Noriyasu Hashida, Ichiro Yamashita
Summary: We developed a new electrochemical method for real-time detection of polymerase chain reactions, using a DNA intercalator to enhance charge transfer and monitoring the changes in charge transfer resistance. This simple method has the potential to be a cost-effective and portable PCR device.
ANALYTICAL CHEMISTRY
(2022)
Article
Food Science & Technology
D. Cristiano, M. F. Peruzy, M. Aponte, A. Mancusi, Y. T. R. Proroga, F. Capuano, N. Murru
Summary: The study investigated the detection methods for Yersinia enterocolitica in leafy greens, finding that only ddPCR technology could detect the pathogen in leafy greens contaminated at low levels. Additionally, the incubation time and temperature were shown to significantly impact the survival and concentration of Yersinia enterocolitica in vegetables.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2021)
Article
Chemistry, Analytical
Kazuyuki Nobusawa, Huan-Wen Han, Fumie Takei, Ting-Chieh Chu, Noriyasu Hashida, Ichiro Yamashita
Summary: We developed a new electrochemical impedimetric method for real-time detection of PCR. By adding a specific DNA intercalator and redox mediator, the progress of PCR reaction can be accurately monitored using impedance measurement. This simple method enables cost-effective and portable PCR devices.
ANALYTICAL CHEMISTRY
(2022)
Article
Medicine, General & Internal
Saiful Arefeen Sazed, Mohammad Golam Kibria, Mohammad Shafiul Alam
Summary: The real-time PCR method established in this study efficiently detects and distinguishes five human Plasmodium species with a single protocol, improving sensitivity and specificity. This method can be considered as an alternative to existing molecular diagnosis lineup for malaria in endemic countries.
Article
Biochemistry & Molecular Biology
Christian Urzi, Damian Hertig, Christoph Meyer, Sally Maddah, Jean-Marc Nuoffer, Peter Vermathen
Summary: This study demonstrates the potential of using NMR technology to measure intracellular and extracellular metabolite concentrations in living cells, as well as to distinguish between them. The results show that the method is reliable and sensitive in detecting concentration changes of metabolites in intra- and extracellular compartments under different culture media conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Jennifer J. Labisch, G. Philip Wiese, Kalpana Barnes, Franziska Bollmann, Karl Pflanz
Summary: The study developed a real-time imaging method for quantifying the infectious titer of lentiviral vectors, which showed lower standard deviation and a broader linear range compared to traditional methods, enabling real-time titer results.
Article
Chemistry, Applied
Sonali Mishra, Saurabh Kumar, Mahendra P. Darokar, Karuna Shanker
Summary: Putranjivah is an Indian native medicinal plant used to treat various diseases, with a new triterpene discovered in its bark. Research shows that friedlein and roxburghonol exhibit better anti-plasmodial activity compared to other isolated triterpenes.
NATURAL PRODUCT RESEARCH
(2021)
Article
Environmental Sciences
Mohammad Saleem Khan, Swati Singh, Praveen Pandey, Manoj Semwal, Alok Kalra
Summary: This study focused on menthol mint crop acreage estimation in the Barabanki district of Uttar Pradesh, India, using remote sensing technologies and an adaptive algorithm. Results showed variations in menthol mint cultivation across different regions.
JOURNAL OF THE INDIAN SOCIETY OF REMOTE SENSING
(2021)
Article
Chemistry, Medicinal
Sucheta Singh, Surjeet Verma, Dharmendra K. Yadav, Anant Kumar, Rekha Tyagi, Priyanka Gupta, Dnyaneshwar U. Bawankule, Mahendra P. Darokar, Santosh K. Srivastava, Alok Kalra
Summary: The study isolated a bioactive compound alpha,beta-dehydrocurvularin from fungal endophytes of Catharanthus roseus, which showed strong inhibitory activity against methicillin-resistant Staphylococcus aureus (MRSA) and synergistic interaction with norfloxacin. Moreover, alpha,beta-dehydrocurvularin demonstrated efflux pump inhibitory potential and decreased production of inflammatory cytokines TNF-alpha and IL-6, suggesting its potential as an antimicrobial and anti-inflammatory agent.
CURRENT TOPICS IN MEDICINAL CHEMISTRY
(2021)
Article
Plant Sciences
K. Premalatha, Navya Botlagunta, D. Santhosh, Channayya Hiremath, Rajesh K. Verma, Karuna Shanker, V Sundaresan, Alok Kalra
Summary: The study investigated the use of microbial bioinoculants isolated from medicinal plants to promote the growth of Andrographis paniculata, showing positive effects on plant height, flower initiation, Andrographolide content, and herbage yield. The use of bioinoculants also contributed to reducing fertilizer expenditure and promoting synergistic interactions among bacterial isolates for plant growth, thus providing a sustainable and cost-effective approach for crop cultivation.
JOURNAL OF PLANT NUTRITION
(2021)
Article
Agricultural Engineering
Sucheta Singh, Shiv S. Pandey, Rashmi Tiwari, Alok Pandey, Karuna Shanker, Alok Kalra
Summary: This study successfully increased the content and yield of medicinally important alkaloids in Catharanthus roseus plants by developing different microbial consortia, with varying effects observed in different seasons and cultivars.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Plant Sciences
Tania Ray, Alok Pandey, Shiv S. Pandey, Sucheta Singh, Karuna Shanker, Alok Kalra
Summary: The study identified two endophytes with inhibitory effects on downy mildew in Papaver somniferum, one of which enhanced the plant's resistance to the disease by activating the salicylic acid-dependent defense pathway. The enhanced resistance was achieved by differential expression of transcripts related to signal transduction, protein modification, disease/defense proteins, transcription factors, and phytohormones in the endophyte-treated plants. Enhanced salicylic acid content was observed in the endophyte-treated plants after exposure to the pathogen, shedding light on the molecular mechanisms underlying the resistance.
PHYSIOLOGIA PLANTARUM
(2021)
Article
Plant Sciences
Maneesha Mall, Karuna Shanker, Abdul Samad, Alok Kalra, Velusamy Sundaresan, Ashutosh K. Shukla
Summary: This study investigated the impact of biotic and abiotic factors on vindoline accumulation in Catharanthus roseus, finding that allene oxide cyclase (CrAOC) plays a key role in modulating vindoline content. The expression of CrAOC showed a direct and positive correlation with vindoline accumulation, particularly with genes involved in the conversion of tabersonine to vindoline.
Article
Environmental Sciences
Pratibha Tripathi, Ranu Yadav, Paurabi Das, Asha Singh, Raghavendra Pratap Singh, Premalatha Kandasamy, Alok Kalra, Puja Khare
Summary: The study showed that inoculation of the endophytic bacterium CIMAP-A7 significantly reduced the stress induced by atrazine in Andrographis paniculata, leading to improved plant growth and reduced accumulation of atrazine in plant tissues.
ENVIRONMENTAL POLLUTION
(2021)
Review
Chemistry, Medicinal
Madhumita Srivastava, Priyanka Maurya, Jyotshna, Karuna Shanker
Summary: Clerodendrum viscosum is used in traditional medicines by Asian ethnic communities, with a wide range of pharmacological effects such as anti-inflammatory, antioxidant, and anticancer properties. Over 50 phytochemicals have been identified in C. viscosum, representing diverse classes of secondary metabolites with physiological and phytopharmaceutical significance.
MEDICINAL CHEMISTRY RESEARCH
(2021)
Article
Agricultural Engineering
Nupoor Prasad, Manoj Semwal, Alok Kalra
Summary: This study evaluates the use of hyperspectral indices to determine the optimum harvest time for Mentha arvensis winter crop. Four hyperspectral indices were identified as potential predictors for sucker traits, indicating the optimum harvesting time. The study holds importance for menthol mint farmers in generating additional income during winter months.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Environmental Sciences
R. K. Srivastava, A. K. Singh, R. P. Bansal, A. Pal, P. Khare, R. S. Sharma, S. Kumar, A. Kalra
Summary: Several Asian countries have the tradition of offering flowers, leaves, and fragrant smoke to the Gods and Goddesses at places of worship. However, the improper disposal of flower waste and burning of incense sticks cause air, soil, and water pollution. To tackle this issue, the Council of Scientific and Industrial Research-Central Institute Medicinal and Aromatic Plants (CSIR-CIMAP) in India has developed a technology to make incense sticks and fragrant cones using offered flowers and leaves, reducing environmental pollution.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY
(2023)
Article
Green & Sustainable Science & Technology
Deepak Kumar, Praveen Kumar Sharma, Om Prakash, Shivani Chaturvedi, Suman Singh, Ch Mohan Sai Kumar, Ashween Deepak Nannaware, Alok Kalra, Prasant Kumar Rout
Summary: A green and economical process was developed to separate lignin and hemicellulose from cornmint essential oil using a two-step method. Cellulose was isolated as a solid residue, and enzymatic biotransformation of cellulose to glucose was carried out using Trichoderma fungi. Glucose was then converted to methyl levulinate under specific conditions.
Article
Agricultural Engineering
Pooja Gaur, Feroz Khan, Karuna Shanker
Summary: This study successfully isolated biologically active compounds from Andrographis paniculata root that have the ability to absorb dietary fat, making them potential candidates for obesity management. 7-O-methyl-dihydro wogonin (MDHW) exhibited the strongest pancreatic lipase inhibition activity and showed a synergistic effect with orlistat. MDHW acts as a competitive inhibitor through energy transfer from pancreatic lipase, and the binding interaction between MDHW and pancreatic lipase is hydrophobic. In vitro and in silico studies confirmed the potential of MDHW as an anti-obesity agent for pharmaceutical and food industry applications.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Chemistry, Analytical
Pooja Gaur, Princi Gupta, Neerja Tiwari, Namita Gupta, Karuna Shanker
Summary: A novel HPTLC method was developed for the simultaneous quantification of six bioactive alkaloids in agro-waste of Piper longum. The method demonstrated excellent separation and specificity, and was successfully applied for accurate quantification in Piper longum roots. Additionally, the method was found to be environmentally friendly and energy efficient.
MICROCHEMICAL JOURNAL
(2023)
Article
Agriculture, Multidisciplinary
Pooja Gaur, Neerja Tiwari, Karuna Shanker
Summary: This study demonstrates the potential of using agricultural wastes of Piper longum as antiobesity agents through the extraction of pseudoalkaloids. One of the compounds, pellitorine, showed strong pancreatic lipase inhibitory activity and exhibited synergistic effects with orlistat. The structural compactness of the interaction between pellitorine and pancreatic lipase was confirmed by Fourier transform infrared spectral studies. Molecular docking studies further revealed the binding interaction between pellitorine and the catalytic active amino side residue of the protein. These findings suggest that pellitorine has the potential to be developed as an anti-obesity supplement.
ACS AGRICULTURAL SCIENCE & TECHNOLOGY
(2022)