Article
Biodiversity Conservation
Shuheng Li, Denghang Yu, Hang Zhang, Renhui Li, Fangfang Cai
Summary: A new algal species, Cartusia hunanesis sp. nov., was discovered in a stream in Hunan, China. Two strains (ZJJ02 and ZJJ03) were studied using morphology, ecology, and molecular data (16S rRNA gene and ITS region). Compared to the type species Cartusia fontana, C. hunanesis has only one trichome in the sheath and large granules near the cross wall. Phylogenetic analysis based on the 16S rRNA gene revealed that the investigated strains of C. hunanesis are closely related to C. fontana. Additionally, Cartusia was found to cluster with members of the family Oculatellaceae, including the genera Pegethrix and Elainella. The ITS region of C. hunanesis showed significant differences in secondary structure compared to C. fontana, indicating that it is a novel species.
Article
Biodiversity Conservation
Maria Christodoulou, Matti Wahlsten, Kaarina Sivonen
Summary: Cyanobacteria found in both aquatic and terrestrial environments have been extensively studied in Finland, but the diversity of terrestrial rock-inhabiting cyanobacteria is often ignored. In this study, two Pseudanabaena-like cyanobacterial strains were isolated and characterized using a polyphasic approach. Phylogenetic analyses using both 16S rRNA gene and 16S-23S ITS region revealed better resolution of species relationships, and based on these analyses, two new species were described. The study also highlighted the need for taxonomic revision in the poorly studied cyanobacterial genus Pseudanabaena.
Article
Biodiversity Conservation
Naresh Kumar, Aniket Saraf, Sagarika Pal, Prashant Singh
Summary: In this study, two cyanobacterial strains KUT1-PS and 18C-PS from India were characterized using a polyphasic approach. The morphological characterization indicated that both strains belonged to the genus Cylindrospermum. Phylogenetic analyses at the genus and species levels provided evidence that strain 18C-PS is a representative of Cylindrospermum badium, while strain KUT1-PS is a novel species referred to as Cylindrospermum solincola sp. nov. This study emphasizes the importance of comparing newly sequenced strains with established species for taxonomic interpretations.
Article
Biodiversity Conservation
Fangfang Cai, Shuheng Li, Hang Zhang, Gongliang Yu, Renhui Li
Summary: A new species of cyanobacteria, Nodosilinea hunanesis, was isolated from a freshwater pond in Zhangjiajie National Forest Park, China. It was characterized through a polyphasic study and found to be distinct from other known Nodosilinea species based on morphological, ecological, and molecular data.
Article
Microbiology
Nitin Keshari, Yang Zhao, Sudipta Kumar Das, Tao Zhu, Xuefeng Lu
Summary: The diversity and composition of cyanobacteria in representative hot springs of Yunnan Province, China were explored using a combination of cultivation-independent and -dependent studies. The study identified a total of 19 cyanobacterial taxa with potential biotechnological usage out of 45 genera isolated, showing promise for future taxonomic novelties and high biotechnological potential.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Sophi Marmen, Eduard Fadeev, Ashraf Al Ashhab, Ayana Benet-Perelberg, Alon Naor, Hemant J. Patil, Eddie Cytryn, Yehudit Viner-Mozzini, Assaf Sukenik, Maya Lalzar, Daniel Sher
Summary: Aquaculture facilities such as fishponds have a significant anthropogenic impact on freshwater ecosystems, with high fish biomass leading to increased nutrient load and potential effects on microbial communities and fish health. In an interconnected aquaculture system, natural seasonal cycles have a greater influence on the structure of aquatic bacterial communities compared to aquaculture-related factors. Recurring cyanobacterial blooms in response to different nutrient ratios, possibly influenced by fish feed input magnitude, were observed in the system.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Engineering, Marine
Yinyin Deng, Cuicui Tian, Chuanming Hu, Guangping Xu, Lien Yang, Qinqin Lu, Wei Zhou
Summary: The germplasm bank of economic algae serves as a biological insurance for the cultivation industry, but the red algal germplasm is easily contaminated by filamentous cyanobacteria, hampering its growth. This study utilized cytological observations, 16S rRNA gene analysis, and microscopy to investigate the contamination pattern. It was found that the filamentous cyanobacteria mainly originated from the red algal thallus or surrounding seawater.
JOURNAL OF MARINE SCIENCE AND ENGINEERING
(2022)
Article
Biodiversity Conservation
Wei Chen, Shuyin Li, Yuanzhao Xu, Ruozhen Geng, Gaofei Song, Peiming Ma
Summary: A coccoid-like cyanobacterium isolated from Caohai Lake in China was characterized and named Cyanodorina ovale based on morphological, electron microscopic, and molecular phylogenetic analyses.
Article
Microbiology
Xin-jiang Liu, Huan Yu, Shao-Juan Du, Zong-Jun Du, Meng-Qi Ye
Summary: A new yellow-orange rod-shaped bacterium, Fulvivirga sedimenti sp. nov., was isolated from sediment collected in Weihai, Shandong Province, China. Strain 1062(T) has distinct physiological, biochemical, and chemotaxonomic characteristics.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Microbiology
Zhanbin Sun, Liyin Guo, Yi Yan, Xiaoping Zhang, Jiaxuan Wang, Bo Liu, Jialiang Xu, Qing Ren
Summary: A new Gram-positive bacterium, named Sporosarcina beigongshangi sp. nov., was isolated from pit mud of Baijiu in Sichuan province, China. This bacterium has a wide range of temperature, pH, and salt tolerance for growth, with specific optimal conditions.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Microbiology
Delong Kong, Qingqing Li, Yanzheng Zhou, Yan Wang, Xu Jiang, Zhiye Wang, Zhiyong Ruan
Summary: A novel species of the genus Pseudomonas, named Pseudomonas tumuqii sp. nov., was isolated from greenhouse soil in Beijing, China. This bacterium possesses distinctive morphology, as well as specific genetic and biochemical characteristics.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Microbiology
Delong Kong, Yan Wang, Qingqing Li, Yanzheng Zhou, Xu Jiang, Zhen Xing, Zhiye Wang, Zhiyong Ruan
Summary: In this study, a novel bacterium strain LAMRS1(T) was isolated from a soil sample collected in Hebei Province, PR China. It was classified as a new species in the genus Chryseobacterium based on 16S rRNA gene sequence analysis and other characteristics. The proposed name for this new species is Chryseobacterium subflavum sp. nov.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2022)
Article
Chemistry, Physical
Ayshat M. Bozieva, Makhmadyusuf Kh. Khasimov, Roman A. Voloshin, Maria A. Sinetova, Elena V. Kupriyanova, Sergey K. Zharmukhamedov, Dmitry O. Dunikov, Anatoly A. Tsygankov, Tatsuya Tomo, Suleyman I. Allakhverdiev
Summary: This study aims to investigate the H2-releasing ability of new strains of cyanobacteria and evaluate the effects of the photosystem II inhibitor DCMU on H2 production under light and dark conditions. The results demonstrate that cultures treated with DCMU produced several times more H2 than untreated cells. The highest H2 photoproduction rate of 4.24 mmol H2 (mg Chl a h)-1 was observed in Dolichospermum sp. IPPAS B.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2023)
Article
Microbiology
Wenke Zhang, Xiaoquan Fan, Haobo Shi, Jian Li, Mingqian Zhang, Jin Zhao, Xiaoquan Su
Summary: In this study, a comprehensive method called Qscore was proposed to evaluate the performance of 16S rRNA amplicons in microbial profiling. The method integrated factors such as amplification rate, taxonomic annotation, sequence type, and length. Evaluation of a wide range of microbe species and microbiomes showed that the Qscore-suggested parameters resulted in high precision in microbiome profiling. This study has significant implications for guiding future microbiome studies and enabling the reuse of massive sequence data.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Yi Yan, Xuan Xing, Zhanbin Sun, Jia Li, Shuyue Hao, Jialiang Xu
Summary: Two bacterial strains, REN4(T) and REN4-1, isolated from daqu samples in a baijiu factory in Shanxi, China, were identified as a novel species of the genus Brevibacterium, named Brevibacterium renqingii sp. nov., based on low 16S rRNA gene sequence similarity with known species and distinct physiological and biochemical characteristics.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Environmental Sciences
Meenakshi Srivastava, Arun Kumar Mishra
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2018)
Article
Microbiology
Ekta Verma, Sindhunath Chakraborty, Balkrishna Tiwari, Arun K. Mishra
JOURNAL OF BASIC MICROBIOLOGY
(2018)
Article
Environmental Sciences
E. Verma, S. Singh, Niveshika, A. K. Mishra
INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY
(2019)
Article
Plant Sciences
Manish S. Kaushik, Arun K. Mishra
PHYSIOLOGIA PLANTARUM
(2019)
Article
Biochemistry & Molecular Biology
Niveshika, Shashank Kumar Maurya, Balkrishna Tiwari, Sindhunath Chakraborty, Ekta Verma, Rajnikant Mishra, Arun Kumar Mishra
MOLECULAR BIOLOGY REPORTS
(2019)
Review
Plant Sciences
Samujjal Bhattacharjee, Arun Kumar Mishra
JOURNAL OF EXPERIMENTAL BOTANY
(2020)
Article
Biotechnology & Applied Microbiology
Bhaskar Jyoti Nath, Ekta Verma, Hridip Kumar Sarma, Arun Kumar Mishra, Bhaben Tanti, Dhruva Kumar Jha
JOURNAL OF THE AMERICAN SOCIETY OF BREWING CHEMISTS
(2020)
Article
Biochemistry & Molecular Biology
Surbhi Kharwar, Samujjal Bhattacharjee, Arun Kumar Mishra
Summary: The study explores the impact of sulfate limitations on EPS production and compositions in cyanobacterium Anabaena sp. PCC 7120, revealing that lower sulfate supplementations can increase EPS production with enhanced expression of Alr2882. Analysis of Alr2882 indicates its membrane localisation and potential role in EPS production, with predictions of helical topology and post-translational modifications, showcasing a high level of molecular regulation in Anabaena sp. PCC 7120.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Ekta Verma, Sindhunath Chakraborty, Surbhi Kharwar, Balkrishna Tiwari, Satya Shila Singh, Arun Kumar Mishra
Summary: The study demonstrates the existence of P-II-acetyl-CoA carboxylase interaction in Synechococcus sp. PCC 7942 and suggests a possible adverse impact of nitrogen starvation on this interaction.
JOURNAL OF APPLIED PHYCOLOGY
(2021)
Article
Environmental Sciences
Sindhunath Chakraborty, Arun Kumar Mishra
Summary: The study elucidates the mechanisms of zinc toxicity in Anabaena sphaerica, highlighting its effects on cell accumulation, growth, pigment content, and oxygen evolution rate, as well as hindrance to heterocyst development and nitrogen fixation. Additionally, zinc stress led to inhibition of glutamine synthetase activity, reduced lipid content, and damaged proteins, lipids, and DNA through oxidative stress.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Review
Biology
Surbhi Kharwar, Samujjal Bhattacharjee, Sindhunath Chakraborty, Arun Kumar Mishra
Summary: Understanding of sulfur metabolism in cyanobacteria has been enhanced with a focus on molecular regulation, while knowledge about its evolutionary perspective and dynamics remains limited. This review provides a comprehensive overview of sulfur metabolism in cyanobacteria, emphasizing on the biosynthesis of cysteine and downstream sulfur-containing metabolites, and comparing regulatory mechanisms with other bacteria and plants. The similarity in sulfur assimilation pathway among bacteria, plants, and cyanobacteria, along with differences in regulatory mechanisms, is highlighted, contributing to a better understanding of the importance and evolutionary perspective of this crucial metabolism.
Article
Biotechnology & Applied Microbiology
Surbhi Kharwar, Samujjal Bhattacharjee, Arun Kumar Mishra
Summary: This study conducted a bioinformatic analysis of cyanobacterial SATs and OAS-TLs, revealing that the large genome and morphological complexities favored the acquisition of these genes. Additionally, in silico protein modeling and docking analysis suggested the potential formation of a hetero-oligomeric bi-enzyme cysteine synthase complex in cyanobacteria.
Article
Plant Sciences
Surbhi Kharwar, Samujjal Bhattacharjee, Arun Kumar Mishra
Summary: High salinity has negative effects on heterocytous cyanobacterium Anabaena sphaerica, reducing growth dynamics, photosynthesis, and membrane stability. However, the cyanobacterium shows resilience by adjusting physiological and biochemical mechanisms, including increasing enzymatic antioxidants and accumulating osmoprotectants. Additionally, it enhances nitrate assimilation, indicating the ability to withstand high salinity.
ACTA PHYSIOLOGIAE PLANTARUM
(2022)
Article
Environmental Sciences
Sindhunath Chakraborty, Samujjal Bhattacharjee, Balkrishna Tiwari, Tameshwar Jaishwal, Satya Shila Singh, Arun Kumar Mishra
Summary: This study investigates the impact of zinc stress on the proteome of Anabaena sphaerica and reveals the molecular mechanisms of zinc tolerance. The cyanobacteria reduce the expression of certain proteins to conserve energy and enhance the expression of metallothionein and antioxidative proteins to chelate and detoxify excess zinc ions. Additionally, the overexpression of certain enzymes and synthesis of antioxidant proteins suggest cysteine synthesis under sulfur deprivation. A. sphaerica also exhibits high zinc biosorption efficiency, confirming its potential for zinc bioremediation.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Microbiology
Surbhi Kharwar, Arun Kumar Mishra
Summary: This study investigates the effects of sulfate limitation on nitrogen and thiol metabolisms in Anabaena sp. PCC 7120. The results show that sulfate limitation leads to decreased nitrogen-containing compounds and thiol contents in the cells, as well as reduced activities of nitrogen and thiol metabolic enzymes. These findings indicate that sulfate plays a crucial role in nitrogen and thiol metabolisms in Anabaena.
CURRENT MICROBIOLOGY
(2023)