Article
Plant Sciences
Kuang-Teng Wang, Ming-Chang Hong, Yu-Sheng Wu, Tsung-Meng Wu
Summary: Duckweed is one of the smallest flowering plants in the world and is used for livestock as biofactors and feedstuff additives due to its high reproduction rate and biomass. This study successfully established a micropropagation technique and Agrobacterium-mediated transformation in duckweed.
Review
Plant Sciences
Aparajita Choudhury, Manchikatla Rajam
Summary: Genetic transformation in legume plants is an important area of agricultural research. Despite some progress in improving crop yields, challenges such as recalcitrance and genotype-dependence still exist. Future research may focus on overcoming these obstacles and further increasing yields.
PLANT CELL REPORTS
(2021)
Article
Plant Sciences
Huimin Ren, Yan Xu, Xiaohong Zhao, Yan Zhang, Jamshaid Hussain, Fuqiang Cui, Guoning Qi, Shenkui Liu
Summary: Casuarina equisetifolia is widely used for agroforestry plantations for various purposes. This study established an efficient regeneration system using stem segment explants, optimizing callus induction and regeneration processes. Genetic transformation protocol using Agrobacterium tumefaciens was also optimized, leading to improved transformation efficiency to obtain transgenic plants in about 4 months. The findings will pave the way for further genetic manipulation and functional genomics of C. equisetifolia.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Xiaonan Li, Haiyan Li, Yuzhu Zhao, Peixuan Zong, Zongxiang Zhan, Zhongyun Piao
Summary: This study developed a regeneration system for Chinese cabbage and established a simple and efficient genetic transformation method. The method can obtain transformants in a relatively short period of time, paving the way for further gene editing and functional studies.
HORTICULTURAL PLANT JOURNAL
(2021)
Article
Forestry
Shaofei Dang, Lifeng Zhang, Suying Han, Liwang Qi
Summary: A simple and efficient protocol for the genetic transformation of Japanese larch was developed and multiple transgenic cell lines were obtained. However, the expression levels of exogenous genes in the transgenic system were relatively low, possibly due to the large genome of Japanese larch.
Article
Plant Sciences
Shengnan Liu, Yunlu Shi, Fang Liu, Yan Guo, Minhui Lu
Summary: This study presents an optimized transformation system that improves the infection and genetic transformation frequency of immature maize embryos mediated by Agrobacterium tumefaciens. The use of LaCl3 pretreatment enhances the infection frequency, increases the proportion of positive callus, yields more positive regenerated plantlets, and significantly increases the transformation frequency from 8.40% to 17.60%.
PLANT CELL REPORTS
(2022)
Article
Horticulture
Junxia Zhang, Jieping Wu, Xinyi Hao, Yaping Xie, Kai Lv, Weirong Xu
Summary: This study established a stable and reproducible transformation protocol for grapevine, using immature zygotic embryos. The optimized conditions led to a considerable reduction in the time required for transformation and the method was found to be easily accessible and straightforward to implement.
SCIENTIA HORTICULTURAE
(2023)
Article
Plant Sciences
Xiaoping Wang, Shanshan Chen, Haonan Zhang, Ping Luo, Fangping Zhou, Bingshan Zeng, Jianmin Xu, Chunjie Fan
Summary: An efficient adventitious bud regeneration and transformation system of Eucalyptus was established using the genotype E. urophylla x E. grandis DH32-29, providing a powerful tool for genetic improvement and gene function analysis in Eucalyptus.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Agricultural Engineering
Bin Zhang, Xiaomin Zhao, Leyi Zhang, Hongkai Liu, Chuanshu Zhu, Zhiqing Ma
Summary: This study established a transformation system for Tripterygium wilfordii through optimizing the concentrations and combinations of growth regulators. Genetic transformation of T. wilfordii was achieved using Agrobacterium tumefaciens and overexpression of the miltiradiene synthase gene (TwMS) resulted in increased triptolide content. This efficient genetic transformation provides a useful tool for future genetic engineering studies on T. wilfordii.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Plant Sciences
Michelle M. M. Dominguez, Carmen S. S. Padilla, Kranthi K. K. Mandadi
Summary: Developing an efficient transformation system is crucial for genetically engineering recalcitrant crops, especially trees. In this study, we present a stable plant transformation method for citrus genetic engineering using Agrobacterium tumefaciens. The methodology has been optimized for fourteen citrus varieties and includes infection, co-cultivation, selection, and culture media conditions. The transformation efficiency can reach 11%, and transgenic plants can be obtained in as early as six months, depending on the variety. The incorporation of helper virulence genes and antioxidants improves the transformation efficiency of specific recalcitrant cultivars.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Guoqin Liu, Yuan Yuan, Hui Jiang, Ying Bao, Guogui Ning, Liangjun Zhao, Xiaofeng Zhou, Hougao Zhou, Junping Gao, Nan Ma
Summary: An efficient transformation method for rose using Agrobacterium tumefaciens-mediated transformation has been established, with a transformation efficiency of up to 6%. Integration of GFP transgenes into the rose genome was confirmed by PCR, RT-PCR, Southern and Western blotting, as well as fluorescence microscopy analysis.
HORTICULTURAL PLANT JOURNAL
(2021)
Article
Plant Sciences
Xudong Ye, Ashok Shrawat, Edward Williams, Anatoly Rivlin, Zarir Vaghchhipawala, Lorena Moeller, Jennifer Kumpf, Shubha Subbarao, Brian Martinell, Charles Armstrong, M. Annie Saltarikos, David Somers, Yurong Chen
Summary: A novel and efficient maize genetic transformation system using Agrobacterium-mediated transformation of embryo explants from mature seeds has been successfully developed. This system has significant advantages in terms of scalability and flexibility, as it starts with mature seeds and the explants are storable.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Luca Capriotti, Angela Ricci, Barbara Molesini, Bruno Mezzetti, Tiziana Pandolfini, Irene Piunti, Silvia Sabbadini
Summary: Plant genetic transformation is a powerful breeding tool for improving disease tolerance, stress resistance, fruit production, and quality in fruit trees. This study validated the use of cotyledons and hypocotyls derived from somatic embryos as starting explants for in vitro regeneration and transformation trials in grapevines. The optimized protocols resulted in successful generation of transgenic plants.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Agronomy
Tanika Thakur, Kshitija Sinha, Tushpinder Kaur, Ritu Kapoor, Gulshan Kumar, Rupam Kumar Bhunia, Prafull Salvi
Summary: Rice is a crucial staple food crop, and an optimized rice transformation protocol is important for studying gene functions and improving rice crops.
Article
Biochemistry & Molecular Biology
Ke Jin, Na Tian, Jorge Freire da Silva Ferreira, Devinder Sandhu, Lizheng Xiao, Meiyi Gu, Yiping Luo, Xiangqin Zhang, Guizhi Liu, Zhonghua Liu, Jianan Huang, Shuoqian Liu
Summary: Tea plants have low efficiency in Agrobacterium-mediated transformation likely due to compounds in tea leaves interfering with plant-pathogen attachment, essential minerals acquisition, and quorum quenching induction.
Article
Plant Sciences
Sandeep K. Singh, Guru R. Valicherla, Anil K. Bikkasani, Srikanth H. Cheruvu, Zakir Hossain, Isha Taneja, Hafsa Ahmad, Kanumuri S. R. Raju, Neelam S. Sangwan, Shio K. Singh, Anil K. Dwivedi, Mohammad Wahajuddin, Jiaur R. Gayen
Summary: The study aimed to develop a precise method for analyzing Withanone (WN) in rat plasma, and investigate its in-vitro ADME behavior. Results showed that WN exhibited stability and high permeability in simulated gastric and intestinal environments, with high plasma protein binding. CYP phenotyping indicated extensive metabolism by CYP 3A4 and 1A2, though it did not potently inhibit any CYP isoenzymes in rat and human liver microsomes. The findings suggest that WN could be a promising phytochemical for neurological disorders based on its pharmacokinetics and pharmacodynamics.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Plant Sciences
Lokesh Kumar Narnoliya, Neelam Sangwan, Jyoti Singh Jadaun, Shilpi Bansal, Rajender Singh Sangwan
Summary: This study cloned and characterized the recombinant caffeic acid 3-O-methyltransferase gene from Neem, demonstrating its crucial role in ferulic acid biosynthesis. The heterologous expression of the gene in different plant species provided insights into its biological function in plants, highlighting potential applications in metabolic engineering for the biosynthesis of therapeutically important phytomolecules.
Article
Oncology
Deepti Diwan, Lei Cheng, Zeba Usmani, Minaxi Sharma, Nicola Holden, Nicholas Willoughby, Neelam Sangwan, Rama Raju Baadhe, Chenchen Liu, Vijai Kumar Gupta
Summary: The success of conventional cancer therapeutics is limited by antibiotic resistance and lack of specificity in anti-tumor drugs. Microbial-derived bioactive molecules are offering a new strategic solution by selectively targeting cancer cells. This review provides a comprehensive summary of the anti-carcinogenic properties of these molecules and discusses effective therapeutic applications to minimize systemic side effects.
SEMINARS IN CANCER BIOLOGY
(2022)
Article
Engineering, Chemical
Gisha Singla, Parmjit S. Panesar, Rajender S. Sangwan, Meena Krishania
Summary: The study focused on the effect of different packaging materials on the quality and storage stability of vermicelli supplemented with debittered kinnow pulp residue. LDPE pouches showed greater changes in moisture content and hardness during storage, with lower overall acceptability compared to BOPP pouches. BOPP demonstrated potential for commercial packaging of antioxidant-rich extruded food products without adversely impacting their physiochemical and sensory properties.
JOURNAL OF FOOD PROCESS ENGINEERING
(2022)
Article
Plant Sciences
Muktesh Chandra, Shiwani Kushwaha, Bhawana Mishra, Neelam Sangwan
Summary: The essential oil of Ocimum species has been widely used in traditional medicine in India for its pharmacological importance. Recent studies have focused on defining the major genes of phenylpropanoid and terpenoid pathways in Ocimum species. Structural studies revealed significant differences in enzymes of the MVA pathway in two major species of Ocimum, with implications for the regulation of terpenoid biosynthesis.
PLANT GROWTH REGULATION
(2022)
Review
Food Science & Technology
Niharika Kaushal, Minni Singh, Rajender Singh Sangwan
Summary: Flavonoids have therapeutic potential and nutraceutical properties, and are associated with different foods. This review discusses the mechanisms and limitations of imparting nutraceutical properties to flavonoids, and explores the use of nanoformulations to overcome these limitations.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Biochemistry & Molecular Biology
Shama Afroz, Zafar Iqbal Warsi, Kahkashan Khatoon, Neelam S. Sangwan, Feroz Khan, Laiq Ur Rahman
Summary: In this study, a key gene CaHMGS in Centella asiatica was successfully isolated and characterized. The gene showed the highest expression level in the roots and the lowest expression level in the node of the plant. Functional complementation experiment confirmed the functionality of the CaHMGS gene. This study contributes to the enhancement of secondary metabolite production in the plant.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Plant Sciences
Yashdeep Srivastava, Sandhya Tripathi, Bhawana Mishra, Neelam S. Sangwan
Summary: This study cloned and characterized a key enzyme, geranylgeranyl pyrophosphate synthase (WsGGPPS), involved in the terpenoid biosynthetic pathway in Withania somnifera. Overexpression of WsGGPPS resulted in increased levels of gibberellic acid. These findings provide important insights into the regulation of major metabolite biosynthesis in Withania somnifera.
Review
Biotechnology & Applied Microbiology
Nisha Yadav, Deependra Singh, Manju Rawat, Neelam Sangwan
Summary: This paper discusses the global incidence and prevalence of various types of cancer and explores the potential of natural drugs and nanocarrier systems in cancer treatment. It highlights the promising alternatives offered by specialized metabolites from medicinal plants and emphasizes the importance of phytochemicals in preventing and treating cancer. The paper also suggests that nanoparticles can enhance the stability, solubility, bioavailability, and target specificity of plant products, making them more effective and safe for drug delivery.
Article
Multidisciplinary Sciences
Jyoti Sangwan, Sandhya Tripathi, Nisha Yadav, Yogesh Kumar, Neelam Sangwan
Summary: SARS-nCoV, a rapidly spreading coronavirus, has affected millions of people worldwide. Understanding the genomic information is crucial for controlling its spread. Comparative sequence analysis revealed variations in the sequences coding for different proteins between SARS CoV and SARS nCoV. The S protein coding sequences of SARS CoV showed deletions, insertions, and substitutions in SARS nCoV sequence. The binding interaction pattern of ACE2 receptor protein with SARS-CoV-2 and Omicron variant indicated potential impacts on virulence.
PROCEEDINGS OF THE INDIAN NATIONAL SCIENCE ACADEMY
(2023)
Article
Materials Science, Paper & Wood
Vinod Kumar, Devendra Kumar Sharma, Pankaj Preet Sandhu, Jyoti Jadaun, Rajender S. Sangwan, Sudesh Kumar Yadav
Summary: This study aimed to develop an efficient scale-up process for bacterial cellulose production, investigating the productivity and characteristics of a new isolate Acetobacter pasteurianus RSV-4 in whey medium, and using whey as a waste byproduct in the process, achieving promising results.
