Article
Biodiversity Conservation
Ting Hong, Nan Huang, Jiezhang Mo, Yuanhao Chen, Tangcheng Li, Hong Du
Summary: Diatoms are ecologically important phytoplankton that contribute significantly to marine primary production and biogeochemical cycles. This study investigated the transcriptomic and physiological responses of the diatom P. tricornutum to ocean warming, revealing significant changes in cell growth, photosynthesis, and energy metabolism.
ECOLOGICAL INDICATORS
(2023)
Article
Biotechnology & Applied Microbiology
Wenlei Zhang, Wenjun Zhou, Si Jiang, Yanyan Wang, Lin Chen, Guanpin Yang, Tianzhong Liu
Summary: Glucose-utilizing heterotrophic transformant was obtained by expressing the human Gult1 gene in P. tricornutum. Glucose metabolism in the transformant relied mainly on the PPP pathway, with the EMP pathway regularly blocked. Excessive G6P accumulation inhibited the heterotrophic growth of the transformant. Expressing Pfk, G6pd, and Pgl genes enhanced the EMP and PPP pathways, resulting in significantly higher biomass production.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Biochemistry & Molecular Biology
Xinjie Zhao, Liwei Gao, Xiangzhong Zhao
Summary: In this study, a two-step method was used to purify Fucoxanthin from Phaeodactylum tricornutum, resulting in a purity of 92.03% and a recovery rate of 76.67%. The identification and quantification of Fucoxanthin were determined by HPLC and ESI-MS. The purified Fucoxanthin exhibited strong antioxidant properties in vitro.
Article
Plant Sciences
Ben Leyland, Ekaterina Novichkova, Achintya Kumar Dolui, Denis Jallet, Fayza Daboussi, Bertrand Legeret, Zhongze Li, Yonghua Li-Beisson, Sammy Boussiba, Inna Khozin-Goldberg
Summary: Diatoms can accumulate and utilize neutral storage lipids, which are crucial for their dominance in marine ecosystems. A protein called PtACBP has been identified to play a role in lipid metabolism and affect various cellular processes in diatoms.
Article
Biochemistry & Molecular Biology
Debaprasad Koner, Niharika Nag, Parismita Kalita, Aditya K. Padhi, Timir Tripathi, Nirmalendu Saha
Summary: The presence of thioredoxin glutathione reductase (TGR) in the facultative air-breathing catfish, Clarias magur, was identified for the first time. The TGR enzyme showed both TrxR and GR activities, contributing to the maintenance of redox homeostasis and antioxidant defense under oxidative stress induced by environmental stressors. Phylogenetic analysis revealed its relationship to fish TrxRs and distant relation to platyhelminth parasites TGRs. The characterization of C. magur TGR provides insights into its unique adaptation to environmental challenges.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biotechnology & Applied Microbiology
Teng Huang, Fan Hu, Yufang Pan, Chenjie Li, Hanhua Hu
Summary: The model diatom P. tricornutum has efficient CO2-concentrating mechanisms and a complete set of enzymes involved in biochemical CCM C4 pathway. The expression of PPDK was upregulated in high CO2-selected populations and its overexpression promoted cell density increase. Knockdown or knockout of PPDK inhibited growth and photosynthesis.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Multidisciplinary Sciences
Ananya Agarwal, Orly Levitan, Helena Cruz de Carvalho, Paul G. Falkowski
Summary: Unlike most plants, unicellular algae can adapt to changes in irradiance within a few hours to days through an enigmatic signaling pathway that coordinates changes in plastid and nuclear gene expression. Functional studies on the model diatom, Phaeodactylum tricornutum, identified two potential signal transduction molecules responsible for photoacclimation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Agricultural Engineering
Thomas O. Butler, Gloria Padmaperuma, Alessandro M. Lizzul, Joe McDonald, Seetharaman Vaidyanathan
Summary: The study found that using the commercial powdered media Cell-Hi JWP for the cultivation of P. tricornutum outdoors in the UK yielded good performance characteristics and cost-effectiveness. There was a strong positive correlation between fucoxanthin and chlorophyll a content, and a weak inverse correlation between EPA content and temperature. Commensal bacterial counts showed a sinusoidal growth profile with a transition in community dominance observed.
BIORESOURCE TECHNOLOGY
(2022)
Article
Chemistry, Applied
Song Yi Koo, Keum Taek Hwang, Soonjae Hwang, Ki Young Choi, Yun Ji Park, Jae-Hyeong Choi, To Quyen Truong, Sang Min Kim
Summary: Nanoencapsulation significantly improves the bioavailability of fucoxanthin in microalgae. Both types of nanoparticles show controlled release of fucoxanthin and enhance its permeability and absorption in cells and plasma.
Article
Multidisciplinary Sciences
Daniel J. Giguere, Alexander T. Bahcheli, Samuel S. Slattery, Rushali R. Patel, Tyler S. Browne, Martin Flatley, Bogumil J. Karas, David R. Edgell, Gregory B. Gloor
Summary: Phaeodactylum tricornutum, a marine diatom, has a new telomere-to-telomere genome assembly generated using Oxford Nanopore long reads. The study identified 25 nuclear chromosomes and corrected assembly errors, providing new insights for genetic engineering and synthetic biology applications.
Article
Plant Sciences
Vanessa Brisson, Courtney Swink, Jeffrey Kimbrel, Xavier Mayali, Ty Samo, Suzanne M. Kosina, Michael Thelen, Trent R. Northen, Rhona K. Stuart
Summary: The roles of different ecological classes of algal exometabolites in regulating microbial community composition are not well understood. In this study, we identified exometabolites from the diatom Phaeodactylum tricornutum and investigated their potential to influence bacterial abundances. We found that secretion of selective bacterial growth substrates represents one mechanism by which algal exometabolites can modulate bacterial community composition.
Article
Agricultural Engineering
Zhi-Peng Wang, Pei-Kang Wang, Yan Ma, Jia-Xue Lin, Cheng-Long Wang, Yu-Xiang Zhao, Xin-Yue Zhang, Bei-Chen Huang, Shou-Geng Zhao, Lei Gao, Jing Jiang, Hai-Ying Wang, Wei Chen
Summary: The study demonstrated that the addition of Laminaria japonica hydrolysate (LPH) is a feasible and efficient strategy to promote fucoxanthin accumulation. In the LPH group, both fucoxanthin content and biomass were significantly increased.
BIORESOURCE TECHNOLOGY
(2022)
Article
Chemistry, Applied
Rodolphe Dumontier, Corinne Loutelier-Bourhis, Marie-Laure Walet-Balieu, Carole Burel, Alain Mareck, Carlos Afonso, Patrice Lerouge, Muriel Bardor
Summary: Microalgae, such as diatoms, are emerging as production systems for recombinant proteins like monoclonal antibodies. Efficient analytical methodologies are required to characterize the N-glycosylation machinery of the host cell and the glycoprotein-based bio-pharmaceuticals produced by microalgae. This study found that the diatom Phaeodactylum tricornutum can synthesize human-like oligomannosides, making it an environmentally friendly and advantageous expression system for producing biopharmaceuticals.
CARBOHYDRATE POLYMERS
(2021)
Article
Biotechnology & Applied Microbiology
Andrew Diamond, Aracely Maribel Diaz-Garza, Jessica Li, Samuel S. Slattery, Natacha Merindol, Elisa Fantino, Fatma Meddeb-Mouelhi, Bogumil J. Karas, Simon Barnabe, Isabel Desgagne-Penix
Summary: The efficacy and limitations of the episome expression system in Phaeodactylum tricornutum were studied, revealing issues such as episome rearrangement and toxicity of fusion proteins.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Biotechnology & Applied Microbiology
Ting-Bin Hao, Yang Lu, Zhong-Hong Zhang, Si-Fen Liu, Xiang Wang, Wei-Dong Yang, Srinivasan Balamurugan, Hong-Ye Li
Summary: This study successfully improved carotenoid production in diatom Phaeodactylum tricornutum through genetic modification, particularly by overexpressing key carotenogenic genes involved in the MEP pathway. The elevated expression of carotenogenic genes led to increased total carotenoid content, with significant enhancement in fucoxanthin content in the engineered lines CMK and CMS.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Review
Pharmacology & Pharmacy
Maria Dolores Pineyro, Diego Arias, Adriana Parodi-Talice, Sergio Guerrero, Carlos Robello
Summary: Chagas Disease, African sleeping sickness, and leishmaniasis are neglected diseases caused by pathogenic trypanosomatid parasites, which have significant impact in poor countries. Current treatment drugs are toxic, difficult to access, and may lead to drug resistance. Inhibiting essential parasite metabolic pathways unique from the host can lead to more efficient and safe drug development.
CURRENT PHARMACEUTICAL DESIGN
(2021)
Article
Biochemistry & Molecular Biology
Natalia Sasoni, Matias D. Hartman, Sergio A. Guerrero, Alberto A. Iglesias, Diego G. Arias
Summary: This study identified MsrAs and MsrB enzymes in Leptospira interrogans, which are involved in the reduction of methionine sulfoxide and play a role in repairing oxidized macromolecules, supporting the existence of a redox metabolism pathway in this pathogen.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2021)
Article
Biochemistry & Molecular Biology
Robertino J. Muchut, Rodrigo D. Calloni, Diego G. Arias, Agustin L. Arce, Alberto A. Iglesias, Sergio A. Guerrero
Summary: Paramylon accumulation in Euglena gracilis cells is closely related to the activity of UDP-glucose pyrophosphorylase and UDP-sugar pyrophosphorylase, with knockdown of these enzymes resulting in a substantial decrease in polysaccharide synthesis.
Article
Biochemistry & Molecular Biology
Maria Ferretti, Rania A. Hussien, Miguel A. Ballicora, Alberto A. Iglesias, Carlos M. Figueroa, Matias D. Asencion Diez
Summary: This study characterized the ADP-GlcPPase enzymes from Melainabacteria and revealed their activity and regulation, providing important insights into the evolution of this enzyme family and the phylogenetic position of Melainabacteria.
Article
Biochemistry & Molecular Biology
Natalia Sasoni, Matias D. Hartman, Guillermo Garcia-Effron, Sergio A. Guerrero, Alberto A. Iglesias, Diego G. Arias
Summary: This study reports the identification of two new redox factors, Lin1CGrx and Lin2CGrx, linked to GSH metabolism and iron homeostasis in L. interrogans. The biochemical characterization, kinetic and structural analysis of these proteins revealed their functions and structural features.
Review
Biochemistry & Molecular Biology
Carlos M. Figueroa, Matias D. Asencion Diez, Miguel A. Ballicora, Alberto A. Iglesias
Summary: This review summarizes the research conducted over the past two decades on various aspects of ADP-glucose pyrophosphorylase, an important enzyme in starch biosynthesis. The review focuses on the enzyme's structure, regulation, and evolution, as well as its post-translational modifications and subcellular localization. The findings of this research could potentially lead to improvements in crop yield and resilience, which is particularly crucial in the face of climate change and food shortages.
PLANT MOLECULAR BIOLOGY
(2022)
Article
Plant Sciences
Romina Minen, Jaina A. Bhayani, Matias D. Hartman, Antonela E. Cereijo, Yuanzhang Zheng, Miguel A. Ballicora, Alberto A. Iglesias, Dali Liu, Carlos M. Figueroa
Summary: This study presents the crystal structures of key enzymes involved in sugar alcohol synthesis in celery and peach. The findings highlight the relationship between the structure and function of these enzymes, providing important insights into the process of sugar alcohol synthesis in plants.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Microbiology
Jaina Bhayani, Maria Josefina Iglesias, Romina I. Minen, Antonela E. Cereijo, Miguel A. Ballicora, Alberto A. Iglesias, Matias Asencion D. Diez
Summary: We explored the ability of ADP-glucose pyrophosphorylase (ADP-Glc PPase) from different bacteria to use glucosamine (GlcN) metabolites as a substrate or allosteric effectors. The enzyme from Kocuria rhizophila and Rhodococcus spp. exhibited sensitivity to allosteric activation by GlcN-6P when producing ADP-Glc from glucose-1-phosphate (Glc-1P) and ATP. GlcN-6P had a more modest effect on the enzyme from other Actinobacteria, Firmicutes, and Proteobacteria groups. The catalytic efficiency of Glc-1P was higher than GlcN-1P, except for the unregulated heterotetrameric protein from Geobacillus stearothermophilus. The results suggest that the ability of ADP-Glc PPases to use GlcN-1P as an alternative substrate occurred during evolution.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Mashael A. Alghamdi, Rania A. Hussien, Yuanzhang Zheng, Hiral P. Patel, Matias D. Asencion Diez, Alberto A. Iglesias, Dali Liu, Miguel A. Ballicora
Summary: The allosteric regulation of ADP-glucose pyrophosphorylase plays a critical role in glycogen and starch biosynthesis. This study investigates the binding and activation of the enzyme by fructose 6-phosphate (Fru6P) and pyruvate (Pyr). It is found that a sulfate ion in the crystal structure of the enzyme acts as a regulatory site similar to Fru6P. Ser72 interacts with this sulfate ion, and its mutation affects the interaction with Fru6P and enzyme activation. Mutations in Ser72 hinder the binding of Fru6P and the inhibitor AMP, while still allowing activation by Pyr. These findings suggest that ADP-glucose pyrophosphorylase in Agrobacterium tumefaciens has distinct sites for Fru6P and Pyr, working together to regulate glycogen biosynthesis.
Article
Veterinary Sciences
Valeria S. Garcia, Sergio A. Guerrero, Alexis Burna, Ana Demonte, Luis M. Gugliotta, Veronica D. G. Gonzalez
Summary: The aim of this study was to evaluate the performance of a rapid immunochromatographic strip test for the diagnosis of canine visceral leishmaniasis. The results were compared with an in-house ELISA assay, and both tests showed concordant results. The immunochromatographic strip test demonstrated good diagnostic sensitivity (98%) and specificity (95%).
RESEARCH IN VETERINARY SCIENCE
(2023)
Article
Biology
ThankGod Echezona Ebenezer, Ross S. Low, Ellis Charles O'Neill, Ishuo Huang, Antonio DeSimone, Scott C. Farrow, Robert A. Field, Michael L. Ginger, Sergio Adrian Guerrero, Michael Hammond, Vladimir Hampl, Geoff Horst, Takahiro Ishikawa, Anna Karnkowska, Eric W. Linton, Peter Myler, Masami Nakazawa, Pierre Cardol, Rosina Sanchez-Thomas, Barry J. Saville, Mahfuzur R. Shah, Alastair G. B. Simpson, Aakash Sur, Kengo Suzuki, Kevin M. Tyler, Paul V. Zimba, Neil Hall, Mark C. Field
Summary: Euglenoids are unicellular flagellates with wide geographical and ecological distribution. They have biotechnological potential and show promise in fields such as biofuels, nutraceuticals, bioremediation, cancer treatments, and robotics design. However, the lack of reference genomes hinders the development of these applications. The Euglena International Network aims to overcome these challenges.
Article
Immunology
L. I. Ibanez, V. P. Martinez, A. A. Iglesias, C. M. Bellomo, D. O. Alonso, R. M. Coelho, L. Martinez Peralta, N. Periolo
Summary: This study aimed to analyze the differential impact of A(H1N1)pdm09 and A/Victoria/3/75 (H3N2) strains on costimulatory molecules and ProSP-C expression in AECII from BALB/c mice and A549 cell line. The results showed a significant decrease in ProSP-C expression in AECII and A549 cell line with A(H1N1)pdm09 compared to A/Victoria/3/75 (H3N2) and controls (non-infection conditions).
MICROBIAL PATHOGENESIS
(2023)
Article
Plant Sciences
Matias D. Hartman, Bruno E. E. Rojas, Alberto A. A. Iglesias, Carlos M. M. Figueroa
Summary: Plant metabolism is regulated by the allosteric control of enzymes, allowing for complementary and opposite pathways. While high-throughput proteomic studies have gained attention, research on the functions and signaling pathways of post-translational modifications have been limited. This review examines the allosteric regulation of enzymes involved in plant carbon metabolism and discusses recent findings. Additionally, it explores current methods for studying protein-metabolite interactions, which are expected to be crucial for future discoveries.
Article
Chemistry, Multidisciplinary
Alem Storani, Sergio A. Guerrero, Alberto A. Iglesias
Summary: The phosphorolysis of cello-oligosaccharides by Ruminococcus albus is an important process in the rumen, and the cellobiose phosphorylase (RalCBP) and cellodextrin phosphorylase (RalCDP) enzymes from R. albus 8 were characterized in this study. RalCBP showed high activity on cellobiose, while RalCDP extended its activity to longer cello-oligosaccharides. The truncated mutant Ral Delta N63CDP and the chimeric protein RalCDP-CBM37 were also analyzed, revealing the roles of the N-terminal domain and the CBM37 module in the enzyme's function. These enzymes have potential utility in the synthesis and phosphorolysis of cello-saccharides.
FRONTIERS IN CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Lihue N. Gonzalez, Matias S. Cabeza, Carlos Robello, Sergio A. Guerrero, Alberto A. Iglesias, Diego G. Arias
Summary: Trypanosoma cruzi is a unicellular parasite that causes Chagas Disease and infects various mammalian hosts. It exhibits auxotrophy for L-Met and requires the amino acid from the extracellular environment of the host. The study identified a coding sequence for a free-R-MSR enzyme in T. cruzi, which is involved in the reduction of L-MetSO using specific cysteine residues. The results provide new insights into the redox metabolism of T. cruzi and contribute to understanding L-Met metabolism in this parasite.
