Journal
PROTEOMICS CLINICAL APPLICATIONS
Volume 2, Issue 7-8, Pages 964-973Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/prca.200800024
Keywords
CE; database; MS; urine
Funding
- NCRR NIH HHS [M01 RR000032, M01 RR000032-40] Funding Source: Medline
- NIDDK NIH HHS [R56 DK078244, P01 DK061525, R01 DK078244-01, R01 DK078244, P01 DK061525-010001, R01 DK071802-02, R01 DK071802] Funding Source: Medline
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Owing to its availability, ease of collection, and correlation with pathophysiology of diseases, urine is an attractive source for clinical proteomics. However, many proteomic studies have had only limited clinical impact, due to factors such as modest numbers of subjects, absence of disease controls, small numbers of defined biomarkers, and diversity of analytical platforms. Therefore, it is difficult to merge biomarkers from different studies into a broadly applicable human urinary proteome database. Ideally, the methodology for defining the biomarkers should combine a reasonable analysis time with high resolution, thereby enabling the profiling of adequate samples and recognition of sufficient features to yield robust diagnostic panels. CE-MS, which was used to analyze urine samples from healthy subjects and patients with various diseases, is a suitable approach for this task. The database of these datasets compiled from the urinary peptides enables the diagnosis, classification, and monitoring of a wide range of diseases. CE-MS exhibits excellent performance for biomarker discovery and allows subsequent biomarker sequencing independent of the separation platform. This approach may elucidate the pathogenesis of many diseases, and better define especially renal and urological disorders at the molecular level.
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