Article
Biochemistry & Molecular Biology
Artur Pinski, Alexander Betekhtin, Bozena Skupien-Rabian, Urszula Jankowska, Elisabeth Jamet, Robert Hasterok
Summary: High temperature stress induces significant changes in the cell wall proteome of Brachypodium distachyon leaves, resulting in differential accumulation of specific proteins involved in cell wall polysaccharides. The proteomic analysis identified 46 differentially abundant proteins, highlighting decreased protease activity, lignification, and cell wall expansion at 40 degrees Celsius. These findings provide new insights into the response of the cell wall proteome to high temperature stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Safia Saada, Charles Ugochukwu Solomon, Sinead Drea
Summary: The study found that programmed cell death (PCD) occurs in developing grains of the wild model species Brachypodium distachyon. Compared to domesticated species like barley and rice, the rate and pattern of PCD in Brachypodium differ, affecting grain size and shape. This suggests that the regulation of PCD may play a role in determining grain characteristics in different plant species.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Elzbieta Wolny, Aleksandra Skalska, Agnieszka Braszewska, Luis A. J. Mur, Robert Hasterok
Summary: Salt stress affects germination and root growth in Brachypodium, influencing the expression of cell wall expansion-regulating proteins, induction of cell cycle genes, and alterations in genomic and epigenetic mechanisms such as histone acetylation and DNA methylation. These findings suggest a complex molecular response to salt stress in plants, indicating potential avenues for further functional genomics research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Ruben Sancho, Pilar Catalan, Bruno Contreras-Moreira, Thomas E. Juenger, David L. Des Marais
Summary: This study analyzed the transcriptome data of the purple false brome to investigate the differential expression of genes involved in drought adaptation. The study found that genes with variable occupancy in the pan-genome showed different distributions among coexpression modules. A core module related to drought response in B. distachyon was also identified.
Article
Plant Sciences
Yongjie Meng, Kartikye Varshney, Norbert Incze, Eszter Badics, Muhammad Kamran, Sabrina F. Davies, Larissa M. F. Oppermann, Kevin Magne, Marion Dalmais, Abdel Bendahmane, Richard Sibout, John Vogel, Debbie Laudencia-Chingcuanco, Charles S. Bond, Vilmos Soos, Caroline Gutjahr, Mark T. Waters
Summary: In this study, we characterized the developmental functions of KAI2 in the grass Brachypodium distachyon. Our results showed that Bdkai2 mutants exhibited abnormal phenotypes in plant development, including increased internode elongation, reduced leaf chlorophyll levels, increased lateral roots, decreased root hair growth, and impaired root colonization by arbuscular-mycorrhizal fungi. Different hormone analogues showed distinct effects on the transcriptome via BdKAI2. Overall, our findings suggest that KAI2 plays multiple important roles in shoot development, root system development, and transcriptional regulation in grasses.
Article
Plant Sciences
Shoukun Chen, Jiayuan Hou, Yanan Fu, Haifeng Li
Summary: In this study, eight YABBY genes were identified in the Brachypodium distachyon genome and were found to be unevenly distributed. These genes are highly expressed in inflorescences and are regulated by abiotic stresses. Over-expression in Arabidopsis showed different functions in reproductive development and response to cold stress.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Review
Plant Sciences
Julia Mergner, Bernhard Kuster
Summary: Proteins play essential roles in cellular processes, and studying protein expression, interactions, and modifications is crucial for understanding the molecular mechanisms underlying plant phenotypes. Mass spectrometry technology allows for the global identification and quantification of thousands of proteins, revealing dynamic changes in the plant proteome.
ANNUAL REVIEW OF PLANT BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Artur Pinski, Alexander Betekhtin, Jolanta Kwasniewska, Lukasz Chajec, Elzbieta Wolny, Robert Hasterok
Summary: Inhibiting prolyl-4-hydroxylases with 3,4-DHP results in vacuolar-type cell death in roots, highlighting the important role of HRGPs in root hair development and root growth.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Tiago D. G. Nunes, Lea S. Berg, Magdalena W. Slawinska, Dan Zhang, Leonie Redt, Richard Sibout, John P. Vogel, Debbie Laudencia-Chingcuanco, Barbara Jesenofsky, Heike Lindner, Michael T. Raissig
Summary: This study identifies a class III peroxidase, BdPRX76/BdPOX, that plays a role in regulating the size of hair cells and stomata in the model grass Brachypodium distachyon. BdPOX is mainly expressed in hair cells and promotes hair cell size by affecting lignification or crosslinking of related phenolic compounds at the hair cell base. The findings suggest a developmental interplay between hair cells and stomata that optimizes the functionality of the plant epidermis.
Article
Plant Sciences
Quyen T. N. Hoang, Sharanya Tripathi, Jae-Yong Cho, Da-Min Choi, Ah-Young Shin, Suk-Yoon Kwon, Yun-Jeong Han, Jeong-Il Kim
Summary: This study investigated the functions of BdPIL1 and BdPIL3, two PIF-like genes in Brachypodium distachyon, and found that they interact with phytochromes in an active form-specific manner, affecting plant growth and development. Transgenic Brachypodium seedlings with RNAi constructs of BdPIL1 and BdPIL3 showed decreased coleoptile lengths, increased leaf growth, taller stature, and delayed flowering compared to wild-type plants, with downregulation of genes related to cell number regulation, floral induction, and chlorophyll biosynthesis. Additionally, the study revealed the DNA-binding ability of BdPIL1 and BdPIL3 to target promoters was inhibited in the presence of phytochromes, providing insights into the molecular mechanism underlying phytochrome-mediated PIF regulation in Brachypodium.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Cell Biology
Gea Guerriero, Charles Achen, Xuan Xu, Sebastien Planchon, Celine C. Leclercq, Kjell Sergeant, Roberto Berni, Jean-Francois Hausman, Jenny Renaut, Sylvain Legay
Summary: The resurrection species Craterostigma plantagineum possesses remarkable desiccation tolerance due to its unique cell wall folding mechanism, allowing ordered and reversible shrinking of cells. Research using inhibitors affecting cellulose biosynthesis has advanced understanding of compensatory mechanisms. Gene expression and protein abundance in habituated cells did not follow the same trend, suggesting alterations in auxin levels may be responsible for changes in cell wall-related proteins.
Review
Biochemistry & Molecular Biology
Yanmei Chen, Yi Wang, Jun Yang, Wenbin Zhou, Shaojun Dai
Summary: This paper reviews recent breakthroughs and strategies in unraveling the diversity of the plant proteome, focusing on the methods used to analyze posttranslational modifications (PTMs), protein localization, and the organization of proteins into functional modules. It also discusses PTM crosstalk, multiple PTMs temporally regulating protein life cycles, quantitative studies using MS to measure protein turnover rates, and future directions in the study of the plant proteome.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2021)
Article
Plant Sciences
Him K. Shrestha, Yosef Fichman, Nancy L. Engle, Timothy J. Tschaplinski, Ron Mittler, Richard A. Dixon, Robert L. Hettich, Jaime Barros, Paul E. Abraham
Summary: This study investigates the molecular changes resulting from C3H/APX-KD associated lignin modification and negative growth phenotype in Brachypodium distachyon using a multi-omic approach. The results show that reduced C3H/APX alters the abundance of enzymes in the phenylpropanoid biosynthetic pathway, disrupts cellular redox homeostasis, and elicits plant defense responses. A deeper understanding of these pleiotropic phenotypes and potential targets identified in this study could improve the economic feasibility of lignocellulosic biofuel production.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemical Research Methods
Tommy K. Cheung, Chien-Yun Lee, Florian P. Bayer, Atticus McCoy, Bernhard Kuster, Christopher M. Rose
Summary: Single-cell proteomics by mass spectrometry (SCoPE-MS) is a novel method to quantify multiplexed single-cell proteomes, but it faces technical limitations related to isobaric labeling and mass spectrometry. Increasing carrier proteome levels requires a corresponding increase in the number of ions sampled to maintain quantitative accuracy. The introduction of SCPCompanion software assists in rapid evaluation of single-cell proteomic data and recommends parameters for improved data quality.
Article
Plant Sciences
Min Jiang, Guosong Wen, Changling Zhao
Summary: The origin, phylogeny, and function of plant PIFs have been investigated through comprehensive genomic analysis, revealing their important role in plant growth and development.
PLANT CELL REPORTS
(2022)
Article
Cell Biology
Edith Francoz, Philippe Ranocha, Aurelie Le Ru, Yves Martinez, Isabelle Fourquaux, Alain Jauneau, Christophe Dunand, Vincent Burlat
DEVELOPMENTAL CELL
(2019)
Article
Biochemical Research Methods
Isma Belouah, Melisande Blein-Nicolas, Thierry Balliau, Yves Gibon, Michel Zivy, Sophie Colombie
JOURNAL OF PROTEOMICS
(2019)
Article
Plant Sciences
Gregory Guirimand, Anthony Guihur, Catalina Perello, Michael Phillips, Samira Mahroug, Audrey Oudin, Thomas Duge de Bernonville, Sebastien Besseau, Arnaud Lanoue, Nathalie Giglioli-Guivarch, Nicolas Papon, Benoit St-Pierre, Manuel Rodriguez-Concepcion, Vincent Burlat, Vincent Courdavault
Article
Plant Sciences
Sebastien Viudes, Vincent Burlat, Christophe Dunand
PLANT CELL AND ENVIRONMENT
(2020)
Article
Biochemistry & Molecular Biology
Jonathas Pereira Gracas, Philippe Ranocha, Victor Alexandre Vitorello, Bruno Savelli, Elisabeth Jamet, Christophe Dunand, Vincent Burlat
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biochemistry & Molecular Biology
Achraf M. Jemmat, Philippe Ranocha, Aurelie Le Ru, Maxime Neel, Alain Jauneau, Sara Raggi, Simone Ferrari, Vincent Burlat, Christophe Dunand
Article
Cell Biology
Harold Durufle, Philippe Ranocha, Thierry Balliau, Michel Zivy, Cecile Albenne, Vincent Burlat, Sebastien Dejean, Elisabeth Jamet, Christophe Dunand
Article
Cell Biology
Sebastien Viudes, Christophe Dunand, Vincent Burlat
Summary: The study found that different Brassicaceae species exhibit diversity in mucilage traits, which are not directly correlated with the gene sequence conservation in Arabidopsis thaliana's mucilage secretory cell toolbox. Mucilage may be an ancestral feature of the Brassicaceae family, supported by several genes that have not been characterized in Arabidopsis or species-specific genes.
Article
Plant Sciences
Jonathas Pereira Gracas, Mariana Belloti, Joni Esrom Lima, Lazaro Eustaquio Pereira Peres, Vincent Burlat, Elisabeth Jamet, Victor Alexandre Vitorello
Summary: The low pH stress triggers sensitivity responses in roots, leading to cell death or arrest in elongation. The involvement of the cell wall and the spatiotemporal dynamics of these responses in distinct root zones remain poorly understood. The results suggest that low pH-induced disturbances in cell wall integrity trigger sensitive responses within defined root zones.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2021)
Article
Biochemical Research Methods
Louise Badruna, Vincent Burlat, Pierre Roblin, Thomas Enjalbert, Guy Lippens, Immacolata Venditto, Michael J. O'Donohue, Cedric Y. Montanier
Summary: This study focuses on investigating carbohydrate-binding modules using the Jo-In biomolecular welding protein pair. By creating recombinant xylanase variants appended to different carbohydrate-binding modules, the research reveals biochemical properties and lays the foundation for understanding the correlation between enzyme activity and substrate properties.
Article
Multidisciplinary Sciences
Baowen Huang, Guojian Hu, Keke Wang, Pierre Frasse, Elie Maza, Anis Djari, Wei Deng, Julien Pirrello, Vincent Burlat, Clara Pons, Antonio Granell, Zhengguo Li, Benoit van der Rest, Mondher Bouzayen
Summary: The all-flesh type of tomato fruits is caused by mutation of the MBP3 gene, however, knocking down MBP3 in certain genotypes also affect plant and fruit development. Here, the authors show that a natural mutation of AGL11, a close homolog of MBP3, is responsible for the phenotypic divergence.
NATURE COMMUNICATIONS
(2021)
Review
Plant Sciences
Bastien G. Dauphin, Philippe Ranocha, Christophe Dunand, Vincent Burlat
Summary: Plant cell walls show cellular and subcellular specificities, and wall microdomains play a crucial role in macroscopic developmental processes. This study focuses on the contribution of two categories of wall-remodeling molecular actors in wall microdomains.
TRENDS IN PLANT SCIENCE
(2022)
Correction
Multidisciplinary Sciences
Catalina Perello, Ernesto Llamas, Vincent Burlat, Miriam Ortiz-Alcaide, Michael A. Phillips, Pablo Pulido, Manuel Rodriguez-Concepcion
Article
Cell Biology
Hasan Kolkas, Vincent Burlat, Elisabeth Jamet
Summary: Plant primary cell walls are composite structures containing various polysaccharides and proteins. The composition of these cell walls changed during the evolution from algae to terrestrial plants. Through studying the cell wall polysaccharide composition of Marchantia polymorpha, we found pectins, rhamnogalacturonan I, and hemicelluloses such as xyloglucans, mannans, and xylans. These findings provide insights into the evolution and proteome composition of cell walls.
Article
Biochemistry & Molecular Biology
Harold Durufle, Thierry Balliau, Nicolas Blanchet, Adeline Chaubet, Alexandra Duhnen, Nicolas Pouilly, Melisande Blein-Nicolas, Brigitte Mangin, Pierre Maury, Nicolas Bernard Langlade, Michel Zivy
Summary: In this study, the impact of hybridity status on the responses to drought in sunflower was investigated. It was found that hybrids showed better adaptation and more pronounced responses to water deficit at the molecular and eco-physiological levels compared to inbred lines. The presence of more genes and potential defense mechanism in hybrids may explain these differences.