Journal
PROTEOMICS
Volume 9, Issue 23, Pages 5214-5223Publisher
WILEY
DOI: 10.1002/pmic.200900151
Keywords
Blue-native electrophoresis; Clear-native electrophoresis; Protein complex; Protein-protein interaction; Supercomplex; Technology
Funding
- Deutsche Forschungsgemeinschaft
- Sonderforschungsbereich [815]
- Cluster of Excellence Macromolecular Complexes at the Goethe University Frankfurt [EXC 115]
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Permanent protein-protein interactions are commonly identified by co-purification of two or more protein components using techniques like co-immunoprecipitation, tandem affinity purification and native electrophoresis. Here we focus on blue-native electrophoresis, clear-native electrophoresis, high-resolution clear-native electrophoresis and associated techniques to identify stable membrane protein complexes and detergent-labile physiological super-complexes. Hints for dynamic protein-protein interactions can be obtained using two-hybrid techniques but not from native electrophoresis and other protein isolation techniques except after covalent cross-linking of interacting proteins in vivo prior to protein separation.
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