Journal
PROTEOMICS
Volume 9, Issue 3, Pages 580-597Publisher
WILEY
DOI: 10.1002/pmic.200700208
Keywords
Aging; Mouse kidney; Proteomics
Funding
- Keck Graduate Institute of Applied Life Sciences, Claremont, CA
- Arnold and Mabel Beckman Foundation
- Ralph M. Parsons Foundation
- National Science Foundation [FIBR 0527023, EMT 0523643]
- NSF
- FIBR
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Aging is a time-dependent complex biological phenomenon observed in various organs and organelles of all living organisms. To understand the molecular mechanism of age-associated functional loss in aging kidneys, we have analyzed the expression of proteins in the kidneys of young (19-22 wk) and old (24 months) C57/BL6 male mice using 2-DE followed by LC-MS/MS. We found that expression levels of 49 proteins were upregulated (p <= 0.05), while that of only ten proteins were downregulated (p <= 0.05) due to aging. The proteins identified belong to three broad functional categories: (i) metabolism (e.g., aldehyde dehydrogenase family, ATP synthase beta-subunit, malate dehydrogenase, NADH dehydrogenase (ubiquinone), hydroxy acid oxidase 2), (ii) transport (e.g., transferrin), and (iii) chaperone/stress response (e.g., Ig-binding protein, low density lipoprotein receptor-related protein associated protein 1, selenium-binding proteins (SBPs)). Some proteins with unknown functions were also identified as being differentially expressed. ATP synthase P subunit, transferrin, fumarate hydratase, SBPs, and albumin are present in multiple forms, possibly arising due to proteolysis or PTMs. The above functional categories suggest specific mechanisms and pathways for age-related kidney degeneration.
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