Journal
PROTEOMICS
Volume 8, Issue 23-24, Pages 4853-4861Publisher
WILEY-BLACKWELL
DOI: 10.1002/pmic.200800321
Keywords
Protein stain; Proteomic methods; Silver staining; Two-dimensional gel electrophoresis
Funding
- Region Rhone Alpes
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Protein detection methods after electrophoresis have to be sensitive, homogeneous, and not to impair downstream analysis of proteins by MS. Speed, low cost, and user friendliness are also favored features. Silver staining combines many of these features, but its compatibility with MS is limited. We describe here, a new variant of silver staining that is completely formaldehyde-free. Reducing sugars in alkaline borate buffer are used as developers. While keeping the benefits of silver staining, this method is shown to afford a much better performance in terms of compatibility with MS, both in PMF by MALDI and in LC/ESI/MS/MS.
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