Review
Chemistry, Analytical
Negin Fasih Ramandi, Mohammad Faranoush, Alireza Ghassempour, Hassan Y. Aboul-Enein
Summary: The recent success in studying the proteome as a source of biomarkers has revolutionized our understanding of leukemia. The use of mass spectrometry techniques to identify differentially expressed proteins has led to improved diagnosis, prognosis, and treatment strategies for leukemia. This review focuses on the use of various ionization sources and mass analyzers in monitoring different types of leukemia, with MALDI-TOF MS identified as a quick and reliable method for protein analysis. Additionally, the review classifies over 400 proteins and identifies specific biomarkers for acute myeloid leukemia (AML), acute lymphoblastic leukemia (ALL), and other stages of leukemia for future studies.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Kasper K. Krestensen, Ron M. A. Heeren, Benjamin Balluff
Summary: Mass spectrometry imaging (MSI) has evolved from a niche technique to a widely used spatial biology tool. This review provides an overview of state-of-the-art MSI applications in both established and emerging biomedical fields, serving as a reference framework for new researchers in the field.
Article
Chemistry, Analytical
Jia Yi, Yueqing Shen, Yi Yang, Chengpin Shen, Baohong Liu, Liang Qiao, Yan Wang
Summary: By characterizing gingival crevicular fluid (GCF) sediments and identifying potential protein biomarkers using mass spectrometry techniques, this study has contributed to the early diagnosis of periodontitis and understanding of its pathogenesis. The identified biomarkers hold potential value for accurate monitoring and can be utilized by non-specialists for widespread screening.
Article
Chemistry, Analytical
Bingbing Liu, Xuanlin Meng, Kun Li, Jinggong Guo, Zongwei Cai
Summary: This study used MALDI-MSI to investigate the spatial distribution of lipids in cottonseeds, revealing heterogeneous distribution patterns of different lipid species within the seeds. The roles of these lipids in metabolic pathways were analyzed, and the relationship between their spatial distribution and functions was studied. The unique distribution patterns of lipid metabolites revealed by MSI may offer new insights into the spatial compartmentation of lipid metabolism in plants and can potentially aid genetic engineering efforts when combined with transcriptomics and proteomics.
Article
Chemistry, Multidisciplinary
Bindu Y. Srinivasu, Arun Arumugaperumal, Amrita Mitra, Monita Muralidharan, Rajdeep Das, Amit Kumar Mandal
Summary: Nanoparticles have diverse applications in biomedical fields and can influence the expression of natural proteins in living organisms. The immunological response may be triggered by exposure to NP-Fe-4(P2O7)(3).
JOURNAL OF NANOPARTICLE RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Daojiang Yu, Peng Lai, Tao Yan, Kai Fang, Lei Chen, Shuyu Zhang
Summary: MMP2 plays a crucial role in biological processes, but its spatial analysis is challenging due to its large molecular weight and low content. Researchers have developed a specific MMP2 probe to accurately measure its expression in tissues, which may help study the impact of proteases on malignancy.
FRONTIERS IN CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Qiuqin Zhou, Stefano Rizzo, Janina Oetjen, Annabelle Fueloep, Miriam Rittner, Hartmut Gillandt, Carsten Hopf
Summary: Insufficient vacuum stability of matrix chemicals is a major limitation in MALDI mass spectrometry imaging of large tissue sample cohorts. A photo-cleavable caged molecule, DMNB-2,5-DHAP, was designed and synthesized for lipid MALDI-MSI of mouse brain tissue sections. DMNB-2,5-DHAP exhibited vacuum stability in a high vacuum MALDI ion source and its uncaging process resulted in the generation of 2,5-DHAP with the assistance of the built-in laser in the MALDI ion source.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Analytical
Leonidas Mavroudakis, Kyle D. Duncan, Ingela Lanekoff
Summary: A new method incorporating crown ether molecules in the solvent for nano-DESI MSI allows for quantitative imaging of alkali metal ions and endogenous lipids/metabolites. Applied to an ischemic stroke model, this method revealed dynamic tissue sodium and potassium concentrations, along with changes in lipids, neurotransmitters, and amino acids.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Liang Shan, Han Gao, Jing Zhang, Wentao Li, Yue Su, Yinlong Guo
Summary: This study combined MALDI-TOF MS with EM technology to directly analyze exosomal proteins in human plasma and serum. The results showed that EM had higher sensitivity in the high-mass range and characteristic exosomal proteins were identified. Furthermore, when using EM as a detector, there was a good linear relationship between peak intensity and concentration.
Article
Multidisciplinary Sciences
G. Guo, M. Papanicolaou, N. J. Demarais, Z. Wang, K. L. Schey, P. Timpson, T. R. Cox, A. C. Grey
Summary: Spatial proteomics using MALDI-MSI is a powerful tool for direct detection of protein distribution in tissues but faces challenges in peptide quantification and identification. The development of HIT-MAP provides an open-source bioinformatics workflow for accurate protein annotation and spatial mapping from MALDI-MSI data.
NATURE COMMUNICATIONS
(2021)
Article
Biochemical Research Methods
Kaitlin C. Lesco, Steven M. Rowland, S. Kim Ratanathanawongs Williams, Lieve M. L. Laurens
Summary: This study used matrix-assisted laser desorption ionization imaging mass spectrometry to investigate the localization of lipids in immobilized Spirulina filaments. Optimization of matrix selection and deposition methods improved signal abundance and spatial resolution, and allowed for identification of distinct lipid composition regions. This work provides a methodology for micro-ecological untargeted molecular phenotyping.
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2023)
Review
Biochemistry & Molecular Biology
Weifu Ren, Qi Bian, Yan Cai
Summary: Kidney disease is a global health concern that requires upgraded diagnostic tools to diagnose and prevent disease progression at an early stage. N-glycosylation, as an important protein modification, plays a crucial role in renal structure and function. Research on the role of N-glycosylation in kidney disease provides insights into its pathophysiology and paves the way for clinical applications.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Agriculture, Multidisciplinary
Chizumi Abe, Ye Zhang, Kazuhiro Takao, Kuni Sasaki, Koji Ochiai, Toshiro Matsui
Summary: This study visually investigated the dynamic production of soybean phytoalexins glyceollins in germinating soybeans inoculated with Aspergillus oryzae using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) imaging. The results showed that glyceollins were only produced in the seed coat and germinated root regions of soybeans, and inoculation of inactivated fungi also promoted glyceollin production, suggesting a possible association with the fungus body.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Xiangyu Guo, Wenbo Cao, Xiaomin Fan, Zhiying Guo, Donghui Zhang, Haoyue Zhang, Xiaoxiao Ma, Jiahong Dong, Yunfang Wang, Wenpeng Zhang, Zheng Ouyang
Summary: Mass spectrometry imaging (MSI) of lipids in biological tissues can provide valuable information for biological research and disease diagnosis by correlating molecular distribution with pathological results. A new multiplexed MS2 imaging method (MSI-I-2) has been developed, allowing for MS2 images of 10 or more lipid species at each sampling spot, providing spatial structural lipidomic information. Coupled with on-tissue photochemical derivatization, imaging of 20 phospholipid C=C location isomers is also possible, resulting in enhanced molecular images with high structural definition for mouse brain and human liver cancer tissue sections. Spatially mapped t-distributed stochastic neighbor embedding has been utilized to visualize the tumor margin with the assistance of structural lipidomic information.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemical Research Methods
Yarixa L. Cintron-Diaz, Mario E. Gomez-Hernandez, Marthe M. H. A. Verhaert, Peter D. E. M. Verhaert, Francisco Fernandez-Lima
Summary: Recent developments have focused on label-free mass spectrometry analysis of neuropeptides in sections of formalin-fixed, paraffin embedded (FFPE) tissues, with the goal of making the vast collections of human clinical samples archived in biobanks accessible for mass spectrometry imaging (MSI). Besides detecting and localizing neuropeptides and other biomolecular signals in FFPE samples, there is great interest in their molecular identification and full characterization.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)
Article
Biochemistry & Molecular Biology
D. Rossin, I. H. K. Dias, M. Solej, I. Milic, A. R. Pitt, N. Iaia, L. Scoppapietra, A. Devitt, M. Nano, M. Degiuli, M. Volante, C. Caccia, V. Leoni, H. R. Griffiths, C. M. Spickett, G. Poli, F. Biasi
FREE RADICAL BIOLOGY AND MEDICINE
(2019)
Article
Multidisciplinary Sciences
Alvin C. K. Teo, Sarah C. Lee, Naomi L. Pollock, Zoe Stroud, Stephen Hall, Alpesh Thakker, Andrew R. Pitt, Timothy R. Dafforn, Corinne M. Spickett, David I. Roper
SCIENTIFIC REPORTS
(2019)
Article
Biochemistry & Molecular Biology
Isabel Campos-Pinto, Lucia Mendez, James Schouten, John Wilkins, Maria Fedorova, Andrew R. Pitt, Paul Davis, Corinne M. Spickett
FREE RADICAL BIOLOGY AND MEDICINE
(2019)
Article
Biochemistry & Molecular Biology
Zhixu Ni, Bebiana C. Sousa, Simone Colombo, Catarina B. Afonso, Tania Melo, Andrew R. Pitt, Corinne M. Spickett, Pedro Domingues, M. Rosario Domingues, Maria Fedorova, Angela Criscuolo
FREE RADICAL BIOLOGY AND MEDICINE
(2019)
Article
Biochemistry & Molecular Biology
Bebiana C. Sousa, Tanzim Ahmed, William L. Dann, Jed Ashman, Alexandre Guy, Thierry Durand, Andrew R. Pitt, Corinne M. Spickett
FREE RADICAL BIOLOGY AND MEDICINE
(2019)
Article
Oncology
Maciej Sobczak, Andrew R. Pitt, Corinne M. Spickett, Agnieszka Robaszkiewicz
Review
Biochemistry & Molecular Biology
Corinne M. Spickett, Andrew R. Pitt
Review
Biochemical Research Methods
Sarah L. Smith, Andrew R. Pitt, Corinne M. Spickett
Summary: PTEN is a crucial tumor suppressor protein involved in cell metabolism and survival processes. Investigating PTEN interactors is essential for understanding its various cellular functions.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Romina Medeiros, Bebiana Sousa, Silvina Rossi, Catarina Afonso, Luis Bonino, Andrew Pitt, Elizabeth Lopez, Corinne Spickett, Graciela Borthagaray
Summary: Ischemic stroke is a major cause of death and disability globally. Nitration of fibrinogen may be an early event of ischemic stroke, with protein-bound 3-nitrotyrosine potentially serving as a biomarker for diagnosis and prognosis. Mass spectrometry analysis revealed an association between specific tyrosine residues in human fibrinogen and ischemic stroke.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biotechnology & Applied Microbiology
M. Lairon-Peris, S. J. Routledge, J. A. Linney, J. Alonso-Del-Real, C. M. Spickett, A. R. Pitt, J. M. Guillamon, E. Barrio, A. D. Goddard, A. Querol
Summary: Researchers characterized 61 strains of Saccharomyces cerevisiae for ethanol tolerance and identified differences in ethanol sensitivity among different strains. The study revealed variations in membrane fluidity and permeability, suggesting a potential link between lipid composition and ethanol sensitivity.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Genetics & Heredity
Maria Lairon-Peris, Gabriel L. Castiglioni, Sarah J. Routledge, Javier Alonso-del-Real, John A. Linney, Andrew R. Pitt, Josef Melcr, Alan D. Goddard, Eladio Barrio, Amparo Querol
Summary: The study focused on the adaptation of a wine industrial yeast to stress conditions involving high ethanol concentrations. The adapted hybrid strain showed increased tolerance to sulfite and ethanol, but decreased fermentation capacity for glucose and fructose compared to the original strain. Genomic analysis revealed different signals of adaptation and chromosomal aneuploidies in the adapted strain. RNA-sequencing analysis showed differential gene expression related to adaptation signals found in the genome of the adapted strain. Additionally, alterations in the lipid composition of the membrane were reported as part of conserved tolerance mechanisms.
MICROBIAL GENOMICS
(2021)
Article
Biochemical Research Methods
Lien Dejager, Mark Jairaj, Kieran Jones, Timothy Johnson, Sherri Dudal, Yves Dudal, Patrick Shahgaldian, Rita Correro, Jun Qu, Bo An, Richard Lucey, Szabolcs Szarka, Robert Wheller, Alina Pruna, Sarah Kettell, Andrew Pitt, Paul Cutler
Summary: Determining the levels of protein cross-linking catalysed by transglutaminase 2 in different disease states has been a difficult task. However, a new method using LC-MS/MS detection based on multiple reaction monitoring has been developed, allowing for efficient digestion without enzyme contamination and providing a sensitivity of 0.1 ng/mL of epsilon-(gamma-glutamyl) lysine in human urine with precision of less than 20% CV. This approach represents a significant advance in the detection and quantification of epsilon-(gamma-glutamyl) lysine.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Multidisciplinary Sciences
Apilaasha Tharmasothirajan, Josef Melcr, John Linney, Thomas Gensch, Karin Krumbach, Karla Marlen Ernst, Christopher Brasnett, Paola Poggi, Andrew R. Pitt, Alan D. Goddard, Alexandros Chatgilialoglu, Siewert J. Marrink, Jan Marienhagen
Summary: Microbial synthesis of plant polyphenols is a more environmentally friendly option compared to chemical synthesis or plant extraction. However, these polyphenols are usually toxic to microorganisms. This study investigates the accumulation of resveratrol in cell membrane and wall during production, and finds that the membrane rigidifying effect of resveratrol can be attenuated by adding palmitelaidic acid or linoleic acid, leading to increased production.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
John A. Linney, Sarah J. Routledge, Simon D. Connell, Tony R. Larson, Andrew R. Pitt, Elizabeth R. Jenkinson, Alan D. Goddard
Summary: There is a growing interest in using microbial cell factories to produce butanol, which is an industrial solvent, platform chemical, and a cleaner alternative to conventional fossil fuels. However, the toxicity of butanol remains a major obstacle to the implementation and development of these technologies. This study investigated the changes in lipid composition in Clostridium saccharoperbutylacetonicum N1-4 (HMT) during butanol fermentation and found that altering the membrane's lipid composition can increase tolerance to butanol. This information will ultimately inform efforts in strain engineering to produce higher quantities of butanol.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2023)
Article
Medicine, General & Internal
Matt Spick, Katherine Longman, Cecile Frampas, Holly Lewis, Catia Costa, Deborah Dunn Walters, Alex Stewart, Michael Wilde, Danni Greener, George Evetts, Drupad Trivedi, Perdita Barran, Andy Pitt, Melanie Bailey
Summary: The study revealed lower lipid levels in COVID-19 positive patients, indicating dyslipidemia. Sebum sampling showed potential for distinguishing between COVID-19 positive and negative patients based on lipidomic analysis.