Journal
PROTEOMICS
Volume 8, Issue 15, Pages 3124-3138Publisher
WILEY
DOI: 10.1002/pmic.200701018
Keywords
Buruli ulcer; Mycobacterium ulcerans; mycolactone; subproteome
Funding
- Institut Pasteur, Pasteur-Genopole-Ile-de-France
- Deutsche Forschungsgemeinschaft [FZT82]
- Association Francaise Raoul Follereau
- Swiss National Science Foundation
Ask authors/readers for more resources
Mycobacterium ulcerans is the causative agent of Buruli ulcer, a rapidly emerging human disease in which mycolactone, a cytotoxic and immunosuppressive macrocyclic polyketide, is responsible for massive skin destruction. The genome sequencing of M. ulcerans has recently been accomplished (http://genolist.pasteur.fr/Bur-uList/) enabling the first proteome study of this important human pathogen. Here, we present a comprehensive proteome analysis of different subcellular fractions and culture supernatant of in vitro grown M. ulcerans. By a combination of gel-based and gel-free techniques for protein and peptide separation with subsequent analysis by MS, we identified 1074 different proteins, corresponding to 25% of the protein-coding DNA sequence. Interestingly, new information was obtained about central metabolism and lipid biosynthesis, and as many as 192 conserved hypothetical proteins were found. Comparative analysis of the wild-type strain and an isogenic mycolactone-deficient mutant, by 2-DE and iTRAQ labeling of the cytoplasmic fraction, revealed differences in the expression profiles of proteins involved in lipid metabolism and information pathways, as well as stress responses.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available