Structure-based engineering of streptavidin monomer with a reduced biotin dissociation rate

Tamavidin 2-REV: An engineered tamavidin with reversible biotin-binding capability

(2013) Yoshimitsu Takakura et al.   JOURNAL OF BIOTECHNOLOGY

Stable, high-affinity streptavidin monomer for protein labeling and monovalent biotin detection

(2012) Kok Hong Lim et al.   BIOTECHNOLOGY AND BIOENGINEERING

Structural Adaptation of a Thermostable Biotin-binding Protein in a Psychrophilic Environment

(2012) Amit Meir et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structure of Bradavidin – C-Terminal Residues Act as Intrinsic Ligands

(2012) Jenni Leppiniemi et al.   PLoS One

Iterative approach to computational enzyme design

(2012) H. K. Privett et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Expression and purification of E. coli BirA biotin ligase for in vitro biotinylation

(2012) Yifeng Li et al.   PROTEIN EXPRESSION AND PURIFICATION

How the biotin–streptavidin interaction was made even stronger: investigation via crystallography and a chimaeric tetramer

(2011) Claire E. Chivers et al.   BIOCHEMICAL JOURNAL

Engineered Streptavidin Monomer and Dimer with Improved Stability and Function

(2011) Kok Hong Lim et al.   BIOCHEMISTRY

Biotin-assisted folding of streptavidin on the yeast surface

(2011) Kok Hong Lim et al.   BIOTECHNOLOGY PROGRESS

Evolved streptavidin mutants reveal key role of loop residue in high-affinity binding

(2011) Maria L. B. Magalhães et al.   PROTEIN SCIENCE

PHENIX: a comprehensive Python-based system for macromolecular structure solution

(2010) Paul D. Adams et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Computational and mutagenesis studies of the streptavidin native dimer interface

(2010) Cheng-Kuo Hsu et al.   JOURNAL OF MOLECULAR GRAPHICS & MODELLING

A streptavidin variant with slower biotin dissociation and increased mechanostability

(2010) Claire E Chivers et al.   NATURE METHODS

Computational Design of Calmodulin Mutants with up to 900-Fold Increase in Binding Specificity

(2008) Eliyahu Yosef et al.   JOURNAL OF MOLECULAR BIOLOGY

Crystal Structure of Rhizavidin: Insights into the Enigmatic High-Affinity Interaction of an Innate Biotin-Binding Protein Dimer

(2008) Amit Meir et al.   JOURNAL OF MOLECULAR BIOLOGY

Structural coupling between FKBP12 and buried water

(2008) Szilvia Szep et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS