Article
Biochemistry & Molecular Biology
Alessandro Marchetti, Marco Orlando, Marco Mangiagalli, Marina Lotti
Summary: The binding of manganese ion (Mn2+) improves the thermal stability and catalytic efficiency of cold-active enzymes, which are key for adaptation to cold environments.
Article
Microbiology
Casper Bojer Rasmussen, Carsten Scavenius, Ida B. Thogersen, Seandean Lykke Harwood, Oivind Larsen, Gro Elin Kjaereng Bjerga, Peter Stougaard, Jan J. Enghild, Mariane Schmidt Thogersen
Summary: In this study, a novel intracellular subtilisin protease (ISP) P355 derived from the genome of Planococcus halocryophilus Or1 was characterized. P355 was found to be one of the most unstable enzymes but had a higher catalytic rate. Compared to other ISPs (T0099, T0034, and SC), P355 and T0034 showed higher catalytic constants, demonstrating the typical stability-activity trade-off observed in cold-adapted proteases.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Saleem Farooq, Ruqeya Nazir, Shabir Ahmad Ganai, Bashir Ahmad Ganai
Summary: This study isolated a cold-active protease Apr-BO1 from psychrotrophic bacteria, showing optimal activity at 20 degrees C and pH 9.0, making it a potential detergent additive.
SCIENTIFIC REPORTS
(2021)
Review
Biochemistry & Molecular Biology
Gulam Rabbani, Ejaz Ahmad, Abrar Ahmad, Rizwan Hasan Khan
Summary: Microbial lipases are excellent biocatalysts that can catalyze various reactions in both aqueous and non-aqueous media. This review focuses on microbial lipases from different sources (psychrophiles, mesophiles, and thermophiles), highlighting the structural diversity in temperature adaptation and industrial applications. Understanding the molecular mechanisms of temperature adaptation in lipases is of great interest, as extremophilic lipases are widely used in biotransformation reactions with higher yields and unique products.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Medicinal
Guang Yang, Matteo Mozzicafreddo, Patrizia Ballarini, Sandra Pucciarelli, Cristina Miceli
Summary: Cold-adapted enzymes produced by psychrophilic organisms have higher catalytic activities at low temperatures due to amino acid changes in protein sequences that increase molecular flexibility in cold. Comparative analysis of lipases from two marine ciliate species revealed new factors possibly responsible for enhanced enzyme activity at low temperatures, emphasizing the importance of subtle sequence and structural modifications for industrial applications.
Article
Engineering, Environmental
Estefanny Quispe-Cardenas, Shane Rogers
Summary: This study investigated microbial adaptation to high ammonia concentrations during anaerobic digestion under psychrophilic and mesophilic conditions. It found that diversity reduced under psychrophilic conditions when adaptation occurred successfully, while the microbial community became more diverse under mesophilic conditions with ammonia inhibition. The study also identified Methanocorposculum as a robust hydrogenotrophic methanogen at high ammoniacal concentrations under psychrophilic conditions for the first time.
Article
Biotechnology & Applied Microbiology
Zongchao Chen, Pengfei Niu, Xiaomei Ren, Wenlong Han, Ruyu Shen, Min Zhu, Yang Yu, Chan Ding, Shengqing Yu
Summary: Recent studies have shown that the secreted subtilisin-like serine protease SspA in Riemerella anatipestifer is associated with bacterial virulence, proteolysis, and functions as an effector protein of the T9SS system, playing a crucial role in pathogenesis and host evasion.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Laura Garcia-Descalzo, Eva Garcia-Lopez, Cristina Cid
Summary: This study investigates the adaptation of bacterial species to temperature changes and the contribution of genome and environmental variables. The results show that different species employ different strategies and that the genus Shewanella has a greater versatility in adapting to temperature changes compared to Psychrobacter. Chaperones play a pivotal role in the molecular machinery used by these bacteria to respond to temperature variations.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Reza Zolfaghari Emameh, Justina Kazokaite, Bagher Yakhchali
Summary: In this study, the evaluation of subtilisin E from Bacillus subtilis revealed highly conserved amino acid sequences and the impact of point mutations on its efficacy. Genomic analysis indicated that the aprE gene and the yhfN gene are expressed through a sigma A promoter, while system biology methods such as point mutations can improve the stability of the produced subtilisin E.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Biochemistry & Molecular Biology
Jesus Fernandez-Lucas, Ivan Acebron, Ruiying Y. Wu, Yohana Alfaro, Javier Acosta, Pierre A. Kaminski, Miguel Arroyo, Andrzej Joachimiak, Boguslaw P. Nocek, Isabel De la Mata, Jose M. Mancheno
Summary: The study reports the crystal structures and biochemical properties of two novel tetrameric NDTs, including the first psychrophilic NDT. Comparative analysis of the new structural and biochemical data reveals that DpNDT has unique structural motifs that likely contribute to its global stability, and its functional and structural features differ from standard psychrophilic enzymes.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Multidisciplinary Sciences
Olivia N. Pattelli, Dinh Dinh Ly Diec, Wanting Guo, Silvia Russi, Daniel Fernandez
Summary: This study reports the molecular basis, structure, and function of Aspergillus fumigatus oryzin. By using molecular replacement and molecular probing, the crystal structure and catalytic mechanism of oryzin were determined, revealing its substrate binding preferences. Furthermore, the binding of the enzyme to a calcium ion was found to provide structural stability.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Sondes Mechri, Fawzi Allala, Khelifa Bouacem, Ismail Hasnaoui, Hassan Gwaithan, Taha Bilel Chalbi, Ennouamane Saalaoui, Abdeslam Asehraou, Alexandre Noiriel, Abdelkarim Abousalham, Hocine Hacene, Amel Bouanane-Darenfed, Marilize Le Roes-Hill, Bassem Jaouadi
Summary: In this study, a serine alkaline protease named SPSM was purified and characterized from Streptomyces mutabilis strain TN-X30. The SPSM gene was cloned and over-expressed in E. coli BL21(DE3)pLysS and E. coli BL21-AITM strains. The immobilization of SPSM using encapsulation and adsorption-encapsulation approaches and different carriers was investigated. The results showed that the immobilized SPSM exhibited similar characteristics to the soluble form, and the white clay and kaolin used as binders showed great potential in the immobilization of SPSM.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Microbiology
Patricia de Almeida Machado, Pollyanna Stephanie Gomes, Victor Midlej, Elaine Soares Coimbra, Herbert Leonel de Matos Guedes
Summary: The study revealed the potential antileishmanial effect of PF-429242, which induces various cellular alterations in the parasite, such as mitochondrial damage, neutral lipids accumulation, oxidative stress, and autophagy leading to the death of L. infantum. Additionally, PF-429242 treatment modulated host cellular responses that favor the development of an immune response against the parasite.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Qianqian Huang, Kui Zhang, Yu Li, Fei Gan, Xiao-Feng Tang, Bing Tang
Summary: Vpr-like proteases are widely distributed in bacteria and fungi and play important roles in processing lantibiotics, degrading collagen, keratin, and fibrin, and contributing to the pathogenesis of microbes. This study investigates a novel Vpr-like protease (BTV) from thermophilic bacterium Brevibacillus sp. WF146, revealing insights into its maturation and oligomerization mechanisms. The results show that hetero-catalytic maturation of BTV is more efficient than autocatalytic maturation, and the fibronectin type III domains mediate the dimerization of mature BTV while the N-terminal propeptide inhibits the dimerization of the proform.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Genetics & Heredity
Xingyue Jin, Yanhui Liu, Zhimin Hou, Yunfei Zhang, Yunying Fang, Youmei Huang, Hanyang Cai, Yuan Qin, Yan Cheng
Summary: This study identified 54 pineapple SBT genes and found that AcoSBT1.12 plays a role in floral transition, as demonstrated by delayed flowering time in transgenic Arabidopsis overexpressing AcoSBT1.12. Analysis also showed that the expression patterns of different AcoSBTs varied in different above-ground tissues, providing insight into the potential functions of SBT in monocots. Yeast-two hybrid analysis revealed potential interactions between AcoSBT1.12 and several other proteins.
FRONTIERS IN GENETICS
(2021)
Article
Genetics & Heredity
Marina de Leeuw, Maayan Baron, Asher Brenner, Ariel Kushmaro
Article
Biochemistry & Molecular Biology
Orna Almog, Anna Kogan, Marina de Leeuw, Garik Y. Gdalevsky, Rivka Cohen-Luria, Abraham H. Parola
Review
Biochemical Research Methods
Julia Johnke, Yossi Cohen, Marina de Leeuw, Ariel Kushmaro, Edouard Jurkevitch, Antonis Chatzinotas
CURRENT OPINION IN BIOTECHNOLOGY
(2014)
Article
Chemistry, Medicinal
Marina de Leeuw, Ana Gonzalez, Assaf Lanir, Levava Roiz, Patricia Smirnoff, Betty Schwartz, Oded Shoseyov, Orna Almog
JOURNAL OF MEDICINAL CHEMISTRY
(2012)
Article
Multidisciplinary Sciences
Marina de Leeuw, Shlomi Reuveni, Joseph Klafter, Rony Granek
Article
Virology
Marina de Leeuw, Maayan Baron, Oshrit Ben David, Ariel Kushmaro
Article
Biotechnology & Applied Microbiology
Marina de Leeuw, Marta R. A. Matos, Lars Keld Nielsen
Summary: Kinetic models are important for understanding metabolic systems. This paper presents a detailed kinetic model for the central carbon metabolism of Escherichia coli, which has been validated and can be used for studying cellular metabolism and metabolic pathway design.
METABOLIC ENGINEERING
(2023)
Article
Ecology
Julia Johnke, Maayan Baron, Marina de Leeuw, Ariel Kushmaro, Edouard Jurkevitch, Hauke Harms, Antonis Chatzinotas
FRONTIERS IN ECOLOGY AND EVOLUTION
(2017)
Article
Green & Sustainable Science & Technology
Marina de Leeuw, Asher Brenner, Ariel Kushmaro
CLEAN-SOIL AIR WATER
(2017)
Article
Biochemical Research Methods
Marina de Leeuw, Levava Roiz, Patricia Smirnoff, Betty Schwartz, Oded Shoseyov, Orna Almog
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
(2007)