Journal
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
Volume 71, Issue 1, Pages 215-226Publisher
WILEY
DOI: 10.1002/prot.21685
Keywords
calcium-sensing receptor; G protein-coupled receptor; homology modeling; contact-based sequence alignment; ligand docking; replica-exchange molecular dynamics; implicit solvation
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Funding
- NCRR NIH HHS [RR12255] Funding Source: Medline
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A three-dimensional model of the human Calcium-sensing receptor (CaSR) seven transmembrane domain was built via a novel sequence alignment method based on the conserved contacts in proteins using the crystal structure of bovine rhodopsin as the template. This model was tested by docking NPS 2143, the first identified allosteric antagonist of CaSR. In our model, Glu837 plays a critical role in anchoring the protonated nitrogen atom and hydroxy oxygen atom of NPS 2143. The phenyl moiety of the ligand contacts residues Phe668, Pro672, and Ile841. The naphthalene moiety is surrounded by several hydrophobic residues, including Phe684, Phe688, and Phe821. Our model appears to be consistent with all six residues that have been demonstrated to be critical for NPS 2143 binding, in contrast with existing homology models based on traditional sequence alignment of CaSR to rhodopsin. This provides validation of our sequence alignment method and the use of the rhodopsin backbone as the initial structure in homology modeling of other G protein-coupled receptors that are not members of the rhodopsin family.
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