Engineering deamidation-susceptible asparagines leads to improved stability to thermal cycling in a lipase

Residue specific contributions to stability and activity inferred from saturation mutagenesis and deep sequencing

(2014) Arti Tripathi et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Engineering lipase A from mesophilic Bacillus subtilis for activity at low temperatures

(2014) V. Kumar et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Asparagine Deamidation Dependence on Buffer Type, pH, and Temperature

(2013) Amanda L. Pace et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Deamidation of Asparagine to Aspartate Destabilizes Cu, Zn Superoxide Dismutase, Accelerates Fibrillization, and Mirrors ALS-Linked Mutations

(2013) Yunhua Shi et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

What a Difference a Dalton Makes: Bacterial Virulence Factors Modulate Eukaryotic Host Cell Signaling Systems via Deamidation

(2013) E. J. Washington et al.   MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS

Saturation Mutagenesis of Lysine 12 Leads to the Identification of Derivatives of Nisin A with Enhanced Antimicrobial Activity

(2013) Evelyn M. Molloy et al.   PLoS One

Thermal stability engineering of Glomerella cingulata cutinase

(2013) I.-S. Chin et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Extensive deamidation at asparagine residue 279 accounts for weak immunoreactivity of tau with RD4 antibody in Alzheimer’s disease brain

(2013) Ayaho Dan et al.   Acta Neuropathologica Communications

Simultaneous Assessment of Asp Isomerization and Asn Deamidation in Recombinant Antibodies by LC-MS following Incubation at Elevated Temperatures

(2012) Katharina Diepold et al.   PLoS One

Probing protein stability and proteolytic resistance by loop scanning: A comprehensive mutational analysis

(2012) Shoeb Ahmad et al.   PROTEIN SCIENCE

Characterization of deamidation of barstar using electrospray ionization quadrupole time-of-flight mass spectrometry, which stabilizes an equilibrium unfolding intermediate

(2012) Santosh Kumar Jha et al.   PROTEIN SCIENCE

Site-specific deamidation of glutamine: a new marker of bone collagen deterioration

(2012) Nienke L. van Doorn et al.   RAPID COMMUNICATIONS IN MASS SPECTROMETRY

Protein Modification by Deamidation Indicates Variations in Joint Extracellular Matrix Turnover

(2011) Jonathan B. Catterall et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

In Vitro Evolved Non-Aggregating and Thermostable Lipase: Structural and Thermodynamic Investigation

(2011) Md. Zahid Kamal et al.   JOURNAL OF MOLECULAR BIOLOGY

Detection, Evaluation and Minimization of Nonenzymatic Deamidation in Proteomic Sample Preparation

(2011) Piliang Hao et al.   MOLECULAR & CELLULAR PROTEOMICS

Analysis of Isoaspartic Acid by Selective Proteolysis with Asp-N and Electron Transfer Dissociation Mass Spectrometry

(2010) Wenqin Ni et al.   ANALYTICAL CHEMISTRY

Comparative proteome analysis of psychrophilic versus mesophilic bacterial species: Insights into the molecular basis of cold adaptation of proteins

(2009) Raghu Metpally et al.   BMC GENOMICS

Deamidation of Asparagine Residues: Direct Hydrolysis versus Succinimide-Mediated Deamidation Mechanisms

(2009) Saron Catak et al.   JOURNAL OF PHYSICAL CHEMISTRY A

Thermostable Bacillus subtilis Lipases: In Vitro Evolution and Structural Insight

(2008) Shoeb Ahmad et al.   JOURNAL OF MOLECULAR BIOLOGY

Deamidation destabilizes and triggers aggregation of a lens protein, βA3-crystallin

(2008) Takumi Takata et al.   PROTEIN SCIENCE