Article
Biochemistry & Molecular Biology
George C. Markou, Ayako Ohoka, Casim A. Sarkar
Summary: Sea lamprey immunization can generate LRR protein binders similar to globular antibodies found in mammals. A new minimal LRR was discovered through lamprey immunization with the human immunoglobulin G Fc domain. This minimal LRR structure may allow the incorporation of additional functionality within a defined scaffold, making it a valuable tool in protein engineering.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Aline Freville, Benedicte Gnangnon, Annie Z. Tremp, Caroline De Witte, Katia Cailliau, Alain Martoriati, El Moukthar Aliouat, Priyanka Fernandes, Cerina Chhuon, Olivier Silvie, Sabrina Marion, Ida Chiara Guerrera, Johannes T. Dessens, Christine Pierrot, Jamal Khalife
Summary: This study reveals the regulation mechanism of key enzyme PP1 in Plasmodium development, including the formation of PbLRR1 complex and its inhibition on PP1 phosphatase activity. Depletion of PbLRR1 negatively affects oocyst development and controls the phosphorylation status of multiple proteins through interaction with new partners.
Article
Plant Sciences
Baiyu Zhang, Zhichao Zhang, Saijiang Yong, Shuyang Yu, Hui Feng, Maozhu Yin, Wenwu Ye, Yuanchao Wang, Min Qiu
Summary: This study identifies a leucine-rich repeat (LRR) protein that plays a crucial role in the development of Phytophthora sojae zoospores. Its absence leads to reduced mobility and chemotaxis of zoospores, as well as decreased cyst germination and virulence.
Review
Plant Sciences
Hyewon Cho, Jungmo Lee, Eunkyoo Oh
Summary: LRR-RLPs and LRR-RLKs are important transmembrane receptors that play critical roles in plant development and immunity. LRR-RLPs transmit signals by forming complexes with co-receptor BAK1 and adaptor protein SOBIR1.
JOURNAL OF PLANT BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Ezequiel A. Galpern, Jacopo Marchi, Thierry Mora, Aleksandra M. Walczak, Diego U. Ferreiro
Summary: Repeat proteins, made up of tandem copies of similar amino acid stretches, have diverse folding mechanisms that can be predicted from sequence scores.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Paola Turina, Piero Fariselli, Emidio Capriotti
Summary: The study of protein folding is crucial for understanding protein function and the relationship between genetics and phenotypes. K-Pro is a new database that collects experimental kinetic data on monomeric proteins with a two-state folding mechanism. It provides a user-friendly interface for browsing and downloading relevant data.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Miaofen Ye, Peng Kuai, Shuting Chen, Na Lin, Meng Ye, Lingfei Hu, Yonggen Lou
Summary: The study revealed that the simple eLRR protein OsI-BAK1 in rice plays a crucial role in defense against the brown planthopper (BPH), acting as a negative regulator of defense-related genes and influencing BPH parasitism and oviposition. The findings suggest that BPH may exploit OsI-BAK1 as a modulator for its own benefit.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Marie Synakewicz, Rohan S. Eapen, Albert Perez-Riba, Pamela J. E. Rowling, Daniela Bauer, Andreas Weissl, Gerhard Fischer, Marko Hyvonen, Matthias Rief, Laura S. Itzhaki, Johannes Stigler
Summary: In this study, the folding of consensus-designed tetratricopeptide repeats (CTPRs) was investigated using single-molecule optical tweezers. It was found that CTPRs exhibit a spring-like mechanical response and the repeat arrays form from the center toward both termini simultaneously. Most importantly, the protein's superhelical tertiary structure was directly observed.
Article
Biochemistry & Molecular Biology
Louise Laursen, Stefano Gianni, Per Jemth
Summary: This study investigates the folding of a three-domain supramodule from the protein PSD-95, revealing that the PDZ domain folds faster and independently from the SH3-GK tandem. However, concurrent folding of the PDZ domain slows down folding of SH3-GK, resulting in an off-pathway folding intermediate. This contributes to the understanding of multidomain protein folding where individual domains cannot be viewed as separate folding units.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Leonore Novak, Maria Petrosino, Daniele Santorelli, Roberta Chiaraluce, Valerio Consalvi, Alessandra Pasquo, Carlo Travaglini-Allocatelli
Summary: A Phi value analysis was conducted on BRD2(2) to investigate its folding pathway, revealing that the C-terminal region serves as the initial folding nucleus, with the N-terminal region consolidating its structure later in the process. This indicates a hierarchical mechanism of protein folding with non-native interactions playing a significant role.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Plant Sciences
Yongchao Hao, Yinghua Pan, Wuying Chen, Muhammad Abdul Rehman Rashid, Mengyao Li, Naixiu Che, Xu Duan, Yan Zhao
Summary: Wheat has a large and diverse repertoire of NLRs involved in disease resistance. The number of NLRs and cluster size can differ drastically among ecotypes and cultivars. Duplication, especially whole genome duplication, has led to the evolution and diversification of NLR genes in wheat.
Article
Biochemistry & Molecular Biology
Christina S. Kim, Anne M. Brown, Tijana Z. Grove, Felicia A. Etzkorn
Summary: Novel consensus leucine-rich repeat proteins (CLRR4-8) based on the LRR domain of NOD-like receptors show promise in high-affinity binding to bacterial cell wall component MDP. The biphasic binding curves and docking studies suggest multi-MDP binding, indicating potential for future pathogen biosensor design.
Article
Biochemistry & Molecular Biology
Ben M. Smith, Pamela J. E. Rowling, Christopher M. Dobson, Laura S. Itzhaki
Summary: The study investigates the interaction between beta-catenin and TCF7L2, revealing the kinetic characteristics through a fluorescence reporter system and mutagenesis analysis. The results suggest a complex two-site avidity mechanism of binding, with the involvement of transient contacts and alternative dissociation pathways. The study provides insights into the molecular recognition of a long intrinsically disordered region with an elongated repeat-protein surface, involving parallel routes and sequential steps.
Article
Biochemistry & Molecular Biology
Jacob D. Marold, Kevin Sforza, Kathryn Geiger-Schuller, Tural Aksel, Sean Klein, Mark Petersen, Ekaterina Poliakova-Georgantas, Doug Barrick
Summary: A collection of programs is presented for analyzing the thermodynamics of folding of linear repeat proteins using a 1D Ising model. These programs determine intrinsic folding and interfacial coupling free energies, and are designed to analyze Ising parameters for homopolymeric and heteropolymeric constructs. The programs also provide a bootstrap analysis for estimating parameter uncertainty and correlations among fitted parameters. Rigorous statistical analysis is essential for interpreting the fits obtained from the complex Ising analysis of repeat proteins.
Article
Multidisciplinary Sciences
Yuan Liu, Congqing Hao, Wei Zhang, Yuzhou Liu, Sen Guo, Ran Li, Meng Peng, Yawei Xu, Xiaoxin Pei, Haibo Yang, Yintao Zhao
Summary: In this study, it was found that LRRK2 deficiency protected the heart against cardiac remodeling induced by pressure overload by regulating autophagy levels.
JOURNAL OF ADVANCED RESEARCH
(2022)