Article
Immunology
Kavya Gauba, Shruti Gupta, Jyoti Shekhawat, Naveen Dutt, Dharmveer Yadav, Vijaya Lakshmi Nag, Mahadev Rao, Praveen Sharma, Mithu Banerjee
Summary: This study aimed to investigate the association between MnSOD gene polymorphism and enzyme levels and mRNA expression in TB patients. The serum SOD2 levels and gene polymorphism were measured in 87 TB patients and 85 healthy individuals. The results showed that the TB patients had lower serum SOD2 levels and mRNA expression compared to the healthy subjects, but there was no significant difference in enzyme levels and mRNA expression among different genotypes.
MICROBES AND INFECTION
(2023)
Article
Zoology
Diana Medina Felix, Edilmar Cortes Jacinto, Angel Isidro Campa Cordova, Jose Antonio Lopez Elias, Luis Rafael Martinez Cordova, Antonio Luna Gonzalez, Sergio David Leal Soto
Summary: The presence of pathogen agents is a major challenge in shrimp farming. This study found that Dunaliella sp. flour can effectively prevent Vibrio infection, reducing mortality and improving physiological and immune response in shrimp.
JOURNAL OF INVERTEBRATE PATHOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Eira Choudhary, Rishabh Sharma, Pramila Pal, Nisheeth Agarwal
Summary: This study examined the adaptive mechanisms of Mycobacterium tuberculosis (Mtb) under physiological stresses and identified protein changes in response to acid, diamide, and H2O2 treatments. The findings highlight the importance of proteomics in understanding the complex adjustments made by Mtb during infection.
Article
Multidisciplinary Sciences
Xiaowen Wang, Hong Zhang, Russell Sapio, Jun Yang, Justin Wong, Xin Zhang, Jessie Y. Guo, Sharon Pine, Holly Van Remmen, Hong Li, Eileen White, Chen Liu, Megerditch Kiledjian, Dimitri G. Pestov, X. F. Steven Zheng
Summary: The study reveals a crucial role of nuclear SOD1 in ribosome biogenesis and proliferation in KRAS-driven lung cancer.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Applied
Ran Li, Shuhong Li, Zhiguang Chen, Yu Jin, Shulei Li, Song Li, Zhizi Bai
Summary: The study on grass carp stefin A gene reveals its heat and acid-base tolerance, inhibitory effects on enzymes, and highest expression level in the hepatopancreas. The results provide insights into the physical and biochemical properties of grass carp stefin A.
Article
Limnology
J. Scott P. McCain, Erin M. Bertrand
Summary: This study highlights the significant impact of antioxidant systems on the macro and micronutrient stoichiometry in marine phytoplankton. Utilizing diatom proteomic data, the study empirically estimated the contribution of antioxidant systems to micronutrient quota variation. Future research should focus on distinguishing between oxidative stress and gene regulation, as well as investigating how antioxidants influence consistency and variation in micronutrient quotas under different environmental conditions.
LIMNOLOGY AND OCEANOGRAPHY LETTERS
(2022)
Article
Plant Sciences
Yushuai Wang, Aimei Dai, Tian Tang
Summary: This study compared the TE compositions and evolutionary dynamics in the genomes of two mangrove species and found that the pioneer species S. alba possesses more TEs. Although many TEs in S. alba showed differential expression under salt stress, only a few were significantly correlated with nearby genes in expression levels.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Weimin Zhang, Ran Brosh, Laura H. McCulloch, Yinan Zhu, Hannah Ashe, Gwen Ellis, Brendan R. Camellato, Sang Yong Kim, Matthew T. Maurano, Jef D. Boeke
Summary: This study presents a new conditional knockout strategy that enables efficient counter selection in mouse embryonic stem cells. The strategy overwrites counter selectable markers and allows the removal or replacement of wild-type DNA with the desired payload DNA. The restored native gene function in the conditional knockout cells facilitates subsequent mouse development and the generality of the strategy is demonstrated through the knockout of other genes.
Article
Cell Biology
Yoshiyuki Henning, Ursula Sarah Blind, Safa Larafa, Johann Matschke, Joachim Fandrey
Summary: This study reveals that HIF stabilization exacerbates iron-dependent cell death in RPE cells under oxidative stress conditions. The Fenton reaction triggered by increased superoxide dismutase (SOD) activity and elevated intracellular iron levels plays a critical role in this process.
CELL DEATH & DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Inga Staneviciene, Dovydas Levinas, Ilona Sadauskiene, Arunas Liekis, Dale Viezeliene, Lolita Kursvietiene, Rima Naginiene, Dale Baranauskiene, Vaida Simakauskiene, Paulina Vaitkiene, Giedre Miniotaite, Jurgita Sulinskiene
Summary: In this study, the effect of long-term selenomethionine administration on oxidative stress level, antioxidant protein/enzyme activity, mRNA expression, and element levels was investigated in mice. Selenomethionine supplementation significantly increased the levels of selenium in blood, liver, and brain, while affecting the homeostasis of iron, zinc, and copper. It also induced lipid peroxidation in blood and brain, and up-regulated the mRNA expression of antioxidant enzymes in the brain and liver.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Mehtap Bayir, Erdal Ozdemir
Summary: The aim of this study was to investigate the effects of diazinon on the expression and activity of SOD enzyme genes in platyfish liver and gill tissues. It was found that exposure to diazinon increased MDA level and SOD enzyme activity in the liver and gill tissues, while down-regulating the expression of sod genes. The liver was identified as a suitable tissue for further gene expression studies, and phylogenetic and identity/similarity analyses confirmed the conservation of sod genes in platyfish, zebrafish, and humans.
ANIMAL BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Akihisa Matsuyama, Atsushi Hashimoto, Shinichi Nishimura, Minoru Yoshida
Summary: Considering the frequent use of leu1 and ura4 genes in fission yeast, we developed new host-vector systems by editing lys1 and arg3 genes with the CRISPR/Cas9 system. We obtained critical mutations in the ORF region and developed complementary vectors for lys1 and arg3 mutants. By combining these vectors with the integration vector pDUAL, we successfully visualized the localization of three proteins fused with different fluorescent proteins in a single cell. Thus, these vectors enable combinatorial expression of heterologous genes, addressing the increasingly diverse experimental challenges.
SCIENTIFIC REPORTS
(2023)
Article
Environmental Sciences
L. M. Odetti, M. F. Simoniello, G. L. Poletta
Summary: The aim of this study was to measure the expression levels of antioxidant response genes Catalase (cat) and copper, zinc Superoxide dismutase (Cu, Zn-sod) in Caiman latirostris (broad-snouted caiman) blood after exposure to cypermethrin (CYP), chlorpyrifos (CPF), glyphosate (GLY), and their binary and ternary mixtures during embryonic development. The results showed a decrease in the expression of sod and cat genes in the three binary mixtures compared to the controls. There was also a possible antagonistic effect between different pesticides in the ternary mixture on the expression of both genes.
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Zahra Karimi Mazraeh Shahi, Zeinab Takalloo, Jahangir Mohamadzadeh, Reza H. Sajedi, Kamahldin Haghbeen, Saeed Aminzadeh
Summary: This study investigated an iron-containing type of superoxide dismutase from a novel thermophilic bacteria, showing good stability and broad catalytic activity. The enzyme could potentially have various industrial applications in cosmetic, food, and pharmaceutical industries.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Multidisciplinary Sciences
Shivang Hina-Nilesh Joshi, Chentao Yong, Andras Gyorgy
Summary: The ability to externally control gene expression has been important for all areas of biological research, especially for synthetic biology. Here the authors present plasmid TULIP which offers DNA copy number control via chemical induction to accelerate the design, prototyping, and reuse of gene circuits in diverse contexts.
NATURE COMMUNICATIONS
(2022)