Article
Multidisciplinary Sciences
Isabel Weigand, Cristina L. Ronchi, Jens T. Vanselow, Kerstin Bathon, Kerstin Lenz, Sabine Herterich, Andreas Schlosser, Matthias Kroiss, Martin Fassnacht, Davide Calebiro, Silviu Sbiera
Summary: Mutations in the PRKACA gene are a common cause of cortisol-producing adrenocortical adenomas and Cushing's syndrome, affecting the binding of regulatory subunits and leading to reduced RII β protein levels. The study shows that phosphorylation of RII β at Ser(114) is necessary for its degradation, mediated by caspase 16, resulting in increased cortisol secretion in adrenocortical cells. These findings provide insight into the molecular mechanisms and pathophysiological relevance of R subunit degradation caused by PRKACA mutations in adrenal Cushing's syndrome.
Article
Biochemistry & Molecular Biology
Grace E. Peng, Veronica Pessino, Bo Huang, Mark von Zastrow
Summary: This study investigates how cAMP produced by endosomes regulates signaling to the nucleus, revealing that the key location-dependent step for endosome-encoded transcriptional control is the nuclear entry of cAMP-dependent protein kinase (PKA) catalytic subunits.
NATURE CHEMICAL BIOLOGY
(2021)
Article
Physics, Multidisciplinary
Tomasz Skora, Mathijs Janssen, Andreas Carlson, Svyatoslav Kondrat
Summary: Macromolecular crowding can have significant impacts on various biophysical processes, including diffusion, gene expression, cell growth, and senescence. However, the effects of crowding on reactions, especially multivalent binding, are not fully understood. In this study, we used scaled particle theory and molecular simulation to investigate the binding of monovalent to divalent biomolecules. Our findings show that crowding can greatly enhance or reduce cooperativity, depending on the sizes of the involved molecular complexes. Furthermore, crowding can enable binding that would not occur otherwise.
PHYSICAL REVIEW LETTERS
(2023)
Article
Multidisciplinary Sciences
Vanessa de Jesus, Nusrat S. Qureshi, Sven Warhaut, Jasleen K. Bains, Marina S. Dietz, Mike Heilemann, Harald Schwalbe, Boris Fuertig
Summary: Translational riboswitches are RNA regulators that modulate gene expression during translation initiation by interacting with 30S ribosome protein S1. The ligand-induced allosteric switch in adenine-sensing riboswitch is insufficient on its own and requires the interaction to fully open the translation initiation region.
NATURE COMMUNICATIONS
(2021)
Article
Environmental Sciences
Zhenjie Sun, Wenhui Gu, Zezhong Feng, Yaqin Fan, Jianfeng Niu, Guangce Wang
Summary: This study elucidated the expression regulation mechanism of the heat shock protein 70 gene in Neopyropia yezoensis under high temperature stress. The findings showed the involvement of cAMP-dependent protein kinase and activating transcription factors element in the transcription regulation of the gene. In addition, AMPK was also found to play a role in the induction of the gene. Furthermore, posttranscriptional mechanisms were proposed to be involved in the synthesis regulation of the heat shock protein 70-2 protein.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Yusuke Fujino, Takero Miyagawa, Masayuki Torii, Masao Inoue, Yuki Fujii, Hiroki Okanishi, Yoshikatsu Kanai, Ryoji Masui
Summary: A study found that a thermophilic Ser/Thr protein kinase completely unfolds at high temperatures, but is significantly stabilized by its natural ligands ATP and ADP, increasing the melting temperature to 80 degrees Celsius. This striking effect of a substrate on the thermostability of thermophilic proteins has not been reported before.
Review
Biochemistry & Molecular Biology
Susan S. Taylor, Jian Wu, Jessica G. H. Bruystens, Jason C. Del Rio, Tsan-Wen Lu, Alexandr P. Kornev, Lynn F. Ten Eyck
Summary: The Protein Data Bank has played a crucial role in the signaling community for over 30 years, facilitating information sharing between academics and industry. Through the study of protein kinases, particularly cAMP-dependent protein kinase, researchers have made significant discoveries including the importance of crystal packing in biological processes and disease relevance. Integrating crystallography with other techniques is opening up new possibilities for understanding intrinsically disordered regions and dynamic biological processes.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Wei-Hong Xiong, Maozhen Qin, Haining Zhong
Summary: The study shows that myristoylation alone can be sufficient for significant membrane association, concentrating PKA activity and regulating AMPA receptors at neuronal synapses. While membrane affinity of PKA-C can be tuned by charges near the myristoylation site, the net neutral charge of PKA-C N terminus is evolutionally conserved.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Analytical
Krishnakumar Chullipalliyalil, Khaled Elkassas, Michael A. P. McAuliffe, Sonja Vucen, Abina Crean
Summary: Conventional quality control techniques for assessing biopharmaceutical denaturation are destructive and require sample removal. In this study, a lab-based setup was used to analyze the heat- and surfactant-induced denaturation of bovine serum albumin (BSA) stored in-vial by measuring its intrinsic fluorescence. This in-vial fluorescence measurement proved to be a reliable method for monitoring protein denaturation, offering a potential cost-effective solution for assessing biopharmaceutical product stability.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Eunyoung Lee, Zhenan Liu, Nhu Nguyen, Angus C. Nairn, Audrey N. Chang
Summary: In this study, the researchers identified the main isoform of PP1c beta expressed in cardiac myocytes and investigated its role in maintaining RLC phosphorylation in vivo. They found that cardiac muscle pathogenesis in PP1c beta knockout animals involves upregulation of total PP1c alpha in myocytes and non-muscle cells. Additionally, they discovered that phosphorylated myofibrillar cardiac myosin is dephosphorylated by both myosin-targeted and soluble MYPT-independent PP1c beta activities. These findings enhance our understanding of cardiac-MLCP in vivo.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Sathapat Charoenjotivadhanakul, Somsri Sakdee, Chompounoot Imtong, Hui-Chun Li, Chanan Angsuthanasombat
Summary: Lysostaphin endopeptidase is a highly effective enzyme in combating methicillin-resistant Staphylococcus aureus. Two loop residues, Tyr270 in loop 1 and Asn372 in loop 4, were found to be functionally important in lysostaphin activity. Experiments showed that these residues play a crucial role in the staphylolytic activity of lysostaphin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Hannah L. Rutledge, Brian D. Cook, Hoang P. M. Nguyen, Mark A. Herzik, F. Akif Tezcan
Summary: This study reports cryo-electron microscopy structures of the nitrogenase complex prepared under enzymatic turnover conditions. The research reveals that asymmetry plays a critical role in various aspects of the nitrogenase mechanism.
Article
Biochemistry & Molecular Biology
Blair K. A. Willette, Jin-Fan Zhang, Jin Zhang, Nikoleta G. Tsvetanova
Summary: Endosome positioning plays a key role in regulating GPCR signaling and initiating transcriptional responses. Disruption of native endosome positioning inhibits GPCR-dependent transcriptional signaling, highlighting its importance in spatially selective receptor signaling.
NATURE CHEMICAL BIOLOGY
(2023)
Article
Chemistry, Analytical
Qingnan Li, Wenzhi Qiang, Jie Yuan, Lehui Xiao
Summary: The sensitive and accurate detection of biomarkers is crucial in clinical diagnosis and drug discovery. Existing amplification-based methods face challenges of limited reproducibility and high background noise. To solve these problems, a robust plasmonic nanoparticle-coupled single-molecule kinetic fingerprinting (PNP-SMKF) method is developed, allowing ultrasensitive detection of protein kinase A (PKA). This method shows great potential as a versatile platform for enzyme detection and inhibitor screening.
ANALYTICAL CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Duangnapa Kovanich, Teck Yew Low, Manuela Zaccolo
Summary: cAMP is a second messenger that plays a crucial role in regulating cellular functions. The compartmentalization of cAMP signaling is essential for its specificity, and the formation of dynamic signaling domains is responsible for precise spatiotemporal regulation. Proteomics can be used to identify the components of these domains and define the dynamic landscape of cellular cAMP signaling. Understanding compartmentalized cAMP signaling in physiological and pathological conditions can provide insights into disease mechanisms and guide the development of precision medicine interventions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Organic
Siim Salmar, Morten Vaalma, Henry Vider, Tiina Tenno, Aleksei Kuznetsov, Jaak Jaerv, Ants Tuulmets
JOURNAL OF PHYSICAL ORGANIC CHEMISTRY
(2016)
Article
Biology
Aleksei Kuznetsov, Rait Kivi, Jaak Jarv
COMPUTATIONAL BIOLOGY AND CHEMISTRY
(2016)
Article
Biochemistry & Molecular Biology
Rait Kivi, Karina Solovjova, Tiv Haljasorg, Piret Arukuusk, Jaak Jarv
Article
Biochemistry & Molecular Biology
Rait Kivi, Jaak Jarv
Article
Chemistry, Physical
Ott Kekisev, Peeter Burk, Jaak Jarv
INTERNATIONAL JOURNAL OF QUANTUM CHEMISTRY
(2017)
Article
Multidisciplinary Sciences
Anton Mastitski, Aleksander Abramov, Anneli Kruve, Jaak Jarv
PROCEEDINGS OF THE ESTONIAN ACADEMY OF SCIENCES
(2017)
Article
Multidisciplinary Sciences
Siim Kukk, Peep Miidla, Jaak Jarv
PROCEEDINGS OF THE ESTONIAN ACADEMY OF SCIENCES
(2017)
Article
Chemistry, Organic
Meeli Arujoe, Anu Ploom, Anton Mastitski, Jaak Jaerv
TETRAHEDRON LETTERS
(2017)
Article
Chemistry, Organic
Meeli Arujoe, Anu Ploom, Anton Mastitski, Jaak Jarv
TETRAHEDRON LETTERS
(2018)
Article
Chemistry, Multidisciplinary
Siim Kukk, Jaak Jarv
Article
Chemistry, Medicinal
Siim Kukk, Olavi Loog, Jukka-Veli Hiltunen, Jaak Jarv
ACS MEDICINAL CHEMISTRY LETTERS
(2018)
Article
Chemistry, Organic
Eerold Vellemae, Anton Mastitski, Jaak Jarv, Jukka Veli Hiltunen
ORGANIC PREPARATIONS AND PROCEDURES INTERNATIONAL
(2018)
Article
Chemistry, Organic
Anton Mastitski, Siret Niinepuu, Toiv Haljasorg, Jaak Jarv
ORGANIC PREPARATIONS AND PROCEDURES INTERNATIONAL
(2018)
Article
Chemistry, Multidisciplinary
Ida Rahu, Ott Kekisev, Jaak Jarv, Peeter Burk
Meeting Abstract
Chemistry, Multidisciplinary
Siim Kukk, Jaak Jarv
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
(2017)