Article
Microbiology
Lingling Zhou, Kaiqi Lian, Mengting Wang, Xueyi Jing, Yuanchen Zhang, Jinling Cao
Summary: This study demonstrated that LL-1 has a strong bactericidal effect on E. coli by increasing the permeability of the cell membrane, resulting in leakage of intracellular content. This finding is of great importance for further understanding the antibacterial mechanism of LL-1 against E. coli and its clinical applications.
Article
Biotechnology & Applied Microbiology
Linglong Fu, Mengning Sun, Weizhang Wen, Na Dong, Defa Li
Summary: This study describes an efficient and simple method for extracellular production of Ulp1 tool enzyme using a leaky E. coli strain. It was found that the addition of glycine increased the extracellular production of Ulp1 approximately four fold. The extracellular Ulp1 had high activities for cleaving SUMO fusion proteins and the cleaved peptide pBD2 exhibited antimicrobial activity. This method of extracellular production of tool enzymes is cost-effective and has potential applications in pharmaceutical, industrial, and biotechnological fields.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Junhao Cheng, Marhaba Ahmat, Henan Guo, Xubiao Wei, Lulu Zhang, Qiang Cheng, Jing Zhang, Junyong Wang, Dayong Si, Yueping Zhang, Rijun Zhang
Summary: This study successfully established an efficient production strategy for CLP and evaluated interference factors affecting the biological activity of CLP, paving the way for further exploration in the treatment of multidrug-resistant bacterial infections.
Article
Microbiology
Runzhe Wu, Xunxi Dong, Qiang Wang, Zirui Zhang, Jianhua Wang, Xiao Wang
Summary: The study synthesized D1018M and D1018 peptides with improved activity and found that they exhibited enhanced resistance against proteases; D1018M showed slightly increased antimicrobial activity, and these peptides had low cytotoxicity, strong inhibitory effects on E. coli, and excellent inhibition capacity against LPS-induced proinflammation response.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Food Science & Technology
Jingyi Dai, Ritian Jin, Jialong Gao, Jude Juventus Aweya, Rong Lin, Guiling Li, Shen Yang
Summary: The rising threat of antibiotic-resistant pathogens has led to the exploration of naturally-derived antimicrobial peptides (AMPs) as replacements for chemical preservatives in foodborne pathogen control. In this study, ten peptides were discovered from salt-fermented shrimps via ultra-performance liquid chromatography-mass spectrometry. Among them, PV-Q5 exhibited strong antibacterial activity against Vibrio parahaemolyticus and Escherichia coli, with a minimum inhibitory concentration of 31.25 μg/mL. PV-Q5 also demonstrated an ability to increase bacterial cell membrane permeability and rupture bacterial cell membranes. These findings suggest that PV-Q5 could be used in food preservation to effectively control foodborne pathogenic bacteria.
Article
Microbiology
Linhui Li, Pengfei She, Shasha Liu, Yimin Li, Zehao Li, Yifan Yang, Linying Zhou, Yong Wu
Summary: This study identified a bioactive molecule 0390 with antimicrobial effects against E. coli. Combination treatment of 0390 with SPR741 showed synergistic antimicrobial effects and demonstrated antibacterial activity in vivo, providing a potential treatment option for E. coli infections.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Somashree Bose, Yogita Dahat, Deepak Kumar, Saikat Haldar, Sujoy K. Das
Summary: In this study, a cationic metal nanoparticle-conjugated fusogenic nanoemulsion (CFusoN) was designed for the treatment of Staphylococcus aureus infection. The nanoemulsion showed a killing efficiency of 99.999% against the bacteria, without exhibiting toxicity to red blood cells and fibroblast cells. The CFusoN altered the bacterial surface charge, leading to bacterial death, and solubilized the bacterial membrane lipid bilayer. In an animal model of skin wound infection, CFusoN demonstrated superior wound cleansing efficacy compared to commercial wound cleansers.
MATERIALS HORIZONS
(2023)
Article
Microbiology
Wenjing Yu, Nianzhi Ning, Ying Xue, Yanyu Huang, Feng Guo, Tao Li, Boning Yang, Deyan Luo, Yakun Sun, Zhan Li, Jianxin Wang, Zhili He, Shiwei Cheng, Xingxiao Zhang, Hui Wang
Summary: The chimeric human defensin H4 showed significantly improved antibacterial activity, especially against multidrug resistant strains, and maintained over 80% antibacterial activity at high salt concentration. Moreover, cytotoxicity tests indicated that H4 had similar toxicity to cells at low doses compared to other defensins.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Francesco Scavello, Angela Mutschler, Sophie Helle, Francis Schneider, Sylvette Chasserot-Golaz, Jean-Marc Strub, Sarah Cianferani, Youssef Haikel, Marie-Helene Metz-Boutigue
Summary: The study investigated a designed peptide based on Catestatin to fight against superbug infections, particularly Staphylococcus aureus, showing the potent antibacterial activity of the newly designed peptides.
SCIENTIFIC REPORTS
(2021)
Article
Infectious Diseases
Raffaella Scotti, Bruno Casciaro, Annarita Stringaro, Fabrizio Morgia, Maria Luisa Mangoni, Roberta Gabbianelli
Summary: New strategies are needed to fight against multidrug-resistant bacteria caused by the overuse of antibiotics. Antibiofilm peptides derived from amphibian skin, such as Esc(1-21) and Esc(1-18), have shown potential to treat infections by inhibiting bacterial growth and biofilm formation. These peptides also induce the expression of genes associated with biofilm regulation and stress response in E. coli O157:H7.
Article
Biochemistry & Molecular Biology
Nishal M. Egodawaththa, Amy L. Knight, Jingxuan Ma, D. Andrew Knight, Eric Guisbert, Nasri Nesnas
Summary: Silver nanoparticles conjugated with organic ligands have been successfully synthesized and demonstrated significant antimicrobial activity and antibacterial capabilities against E. coli. The synthesis of functionalized silver nanoparticles is critical for the further development of silver-nanoconjugated antibacterial agents.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Veterinary Sciences
Gustav G. Bruer, Daria Goedecke, Manfred Kietzmann, Jessica Meissner
Summary: The study revealed that florfenicol treatment resulted in increased resistance in E. coli, while combination with antihistamine mepyramine lowered the minimum inhibitory concentration for other bacteria, indicating potential benefits in enhancing antibacterial efficacy and modifying effects on gut microbiota.
VETERINARY SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Weber Beringui Feitosa, Patricia L. Morris
Summary: Mammalian oocytes undergo morphological, structural, and molecular changes during a process called oocyte aging. The SUMO pathway, specifically SUMO-2/3 and the SUMO-specific protease SENP-2, are involved in spindle morphology changes and chromosome movements during oocyte aging. Decreased UBC9 and differential ubiquitination patterns also correlate with in vitro oocyte aging. These findings suggest that age-related changes in SUMOylation and the deSUMOylation of key target proteins may contribute to spindle and chromosome alignment defects during mammalian oocyte postovulatory aging, with implications for ART-related human oocyte aging and fertilization success.
Article
Infectious Diseases
Yi Wang, Yuchi Song, Shaonan Yan, Rina Hiramine, Yuki Ohnishi, Yuki Yokoi, Kiminori Nakamura, Takashi Kikukawa, Tokiyoshi Ayabe, Tomoyasu Aizawa
Summary: This study investigated the antibacterial mechanisms of Cryptdin-4 in different forms (oxidized and reduced states) under different oxygen conditions using Escherichia coli as a model. The results showed that both forms exhibited antimicrobial activity against bacteria under aerobic conditions through rapid membrane depolarization. Under anaerobic conditions, the ability to disrupt bacterial membrane function decreased, but the reduced form still maintained some antimicrobial activity, possibly through DNA binding inhibition of intracellular functions.
Article
Chemistry, Multidisciplinary
Jordan P. Hempfling, Emily R. Sekera, Amar Sarkar, Amanda B. Hummon, Dehua Pei
Summary: Efficient and convenient method for producing recombinant proteins with free N-terminal cysteines has been reported. Site-specific labeling of the resulting proteins at the N-terminus was achieved through a novel genetic fusion and sequential enzymatic processing.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)