Article
Endocrinology & Metabolism
Gili Solomon, Ewa Oclon, Zvi Hayouka, Arieh Gertler
Summary: Most breast cancer deaths are caused by estrogen receptor-positive breast tumors, which later recur as metastatic disease. Prolactin has been identified as a factor that promotes the development and spread of breast cancer. Researchers have developed superactive prolactin receptor antagonists to block prolactin's actions, and have successfully created variants with extended effects in vivo.
Article
Veterinary Sciences
Timm Konold, John Spiropoulos, Janet Hills, Hasina Abdul, Saira Cawthraw, Laura Phelan, Amy Mckenna, Lauren Read, Sara Canoyra, Alba Marin-Moreno, Juan Maria Torres
Summary: This study demonstrates for the first time that a TSE agent with BSE-like properties can be amplified in cattle inoculated with atypical scrapie brain homogenate.
VETERINARY RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Chu Wu, Limei Qin, Dechao Niu, Xia Wang, Qi Zhang, Yinghui Shang, Min Hu, Xiaoke Han, Saiji Shen, Xing Qin, Songyan Xi, Yongsheng Li, Qigang Wang
Summary: This study presents an efficient multi-pathway singlet oxygen (O-1(2)) generation system for superactive biocatalytic tumor therapy, utilizing compartmental hierarchical nanoreactors. The system achieves increased efficacy by enhancing direct substrate diffusion and reducing external diffusion, showing outstanding capability for O-1(2) production. Both in vitro and in vivo studies demonstrate the synergetic O-1(2)-mediated inhibition of tumor proliferation, showcasing great potential for the treatment of hypoxic tumors.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Endocrinology & Metabolism
Wei Wang, Bo-Tao Zhang, Qi-Lan Jiang, Han-Qing Zhao, Qin Xu, Yang Zeng, Jia-Ying Xu, Jun Jiang
Summary: Allo-aca has been shown to reduce thyroid injury in EAT mice by altering the differentiation of Treg/Th17 cells, providing a novel approach to treating and ameliorating Hashimoto's thyroiditis.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Pharmacology & Pharmacy
Valeria Calcaterra, Vittoria Carlotta Magenes, Virginia Rossi, Valentina Fabiano, Chiara Mameli, Gianvincenzo Zuccotti
Summary: Lipodystrophy is a group of diseases characterized by total or partial lack of body fat, resulting in metabolic derangement and increased cardiovascular risk. This paper focuses on the clinical presentation, treatment options, and the use of recombinant human leptin therapy. Currently, lifestyle modification, medication, and leptin therapy are the main treatment strategies for lipodystrophy, particularly for children and adolescents. Liver-targeted therapies are being investigated as potential new therapeutic strategies for lipodystrophy, especially for patients with partial lipodystrophy.
PHARMACOLOGICAL RESEARCH
(2023)
Article
Oncology
Lisa K. Philp, Anja Rockstroh, Martin C. Sadowski, Atefeh Taherian Fard, Melanie Lehman, Gregor Tevz, Michelle S. Liberio, Charles L. Bidgood, Jennifer H. Gunter, Stephen McPherson, Nenad Bartonicek, John D. Wade, Laszlo Otvos, Colleen C. Nelson
Summary: Hyperleptinaemia is a common side effect of drugs inhibiting the androgen axis in prostate cancer, targeting the leptin axis shows therapeutic potential in advanced PCa, increased intratumoural leptin and LEPR expression is observed in PCa patients' tumours, and preclinical evidence demonstrates the efficacy of targeted leptin-signalling blockade in suppressing tumour growth and delaying progression to CRPC, highlighting the promising new therapeutic strategy of LEPR blockade in combination with androgen axis inhibition in advanced PCa treatment.
ENDOCRINE-RELATED CANCER
(2021)
Article
Endocrinology & Metabolism
Stuart A. Lanham, Dominique Blache, Richard O. C. Oreffo, Abigail L. Fowden, Alison J. Forhead
Summary: Hormones play a crucial role in the regulation of fetal growth and development. A study investigating the effect of pancreas removal on fetal sheep found that pancreas deficiency caused growth retardation and altered bone structure and strength. This highlights the importance of hormones in the development of the fetal skeleton.
JOURNAL OF ENDOCRINOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Dezhi Lin, Hongyu Yang, Xiao Zhang, Huaming Sun, Xuepeng Zhang, Yajun Jian, Weiqiang Zhang, Yunfang Liu, Ziwei Gao
Summary: In this study, a highly selective C-O activation approach using superactive triazine esters and organoborons as coupling partners was developed for the synthesis of α,β-unsaturated ketones with diverse functional groups. Mechanistic investigation revealed that the dual function of triazine for activating the C-O bond and stabilizing non-covalent interactions between the catalyst and substrate is critical for the reaction's success. The method's efficiency, functional group compatibility, and unique mechanism make it a valuable alternative to classic methods.
CHEMICAL COMMUNICATIONS
(2023)
Article
Microbiology
Feng Yue, Lihong Du, Ruyu Wang, Baoquan Han, Xiaojun Zhang, Zhangzhang Yao, Wenqiang Zhang, Chang Cai, Zhiying Zhang, Kun Xu
Summary: This study presents a novel strategy for the therapy of obese individuals with leptin resistance, using in vivo protein interference (iPRTi) to achieve partial leptin reduction. The study confirms the positive effects of this strategy on obesity control by reducing circulating leptin and weight gain in obese mice.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemical Research Methods
Francesca Malerba, Bruno Bruni Ercole, Rita Florio, Antonino Cattaneo
Summary: This article introduces a new bioassay named NTAB for determining the inhibitory potency of NGF-TrkA antagonists, showcasing its advantages over other functional bioassays. The assay was validated with a panel of NGF-TrkA inhibitors, providing useful insights for the prioritization of potential inhibitors.
Article
Biochemical Research Methods
George C. Russell, Ann Percival, Dawn M. Grant, Kathryn Bartley, Dylan Turnbull, Kevin McLean, Julia Lienhard, Claudia Bachofen
Summary: The minor capsid protein of ovine herpesvirus 2 was modified and purified for use as a potential antigen in serological testing, showing promising results in ELISA assays. The recombinant antigen demonstrated high sensitivity and specificity in detecting OvHV-2-specific antibody responses in sheep and cattle samples. This antigen has the potential to be a simple, inexpensive, and reliable test for identifying MCF-affected animals and OvHV-2 reservoir hosts.
JOURNAL OF VIROLOGICAL METHODS
(2022)
Article
Nanoscience & Nanotechnology
Nikolai P. Ponomarev, Mari Kallioinen
Summary: A method using KOH and KCl simultaneously to obtain superactive carbon from lignin was studied, resulting in superactive carbon with an ultra-high surface area and reduced alkali consumption due to the synergy between KOH and KCl.
Article
Mathematics, Applied
Jeong-Ok Choi, Kyuho Jin, Unjong Yu
Summary: Based on a recent study on scientific collaboration networks, this research proposes an induced diffusion percolation model focusing on superactive nodes. Superactive nodes, defined as active nodes with at least k active or superactive neighbors, play a vital role in innovation diffusion by influencing their neighbors to adopt new innovations. The study investigates this induced diffusion percolation model using the modified Newman-Ziff algorithm on two-dimensional lattices (square and triangular) and regular random networks with and without clustering, revealing first-order percolation phase transition in two-dimensional lattices and a double transition in regular random networks.
COMMUNICATIONS IN NONLINEAR SCIENCE AND NUMERICAL SIMULATION
(2023)
Review
Endocrinology & Metabolism
Milan Obradovic, Emina Sudar-Milovanovic, Sanja Soskic, Magbubah Essack, Swati Arya, Alan J. Stewart, Takashi Gojobori, Esma R. Isenovic
Summary: Leptin, a peptide hormone, regulates food intake, body mass, and reproductive function, playing a crucial role in fetal growth, immune responses, angiogenesis, and lipolysis. Leptin resistance can lead to reduced satiety, overconsumption of nutrients, and increased body mass, potentially causing obesity. Combining leptin therapies with sensitizers may help overcome resistance and treat obesity effectively.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Multidisciplinary Sciences
Katarzyna Kopycka, Ben C. Maddison, Kevin C. Gough
Summary: This study demonstrates that specific mutations in ovine rPrP can effectively inhibit the replication and accumulation of PrPSc, with increased inhibitory activity compared to regular rPrP. It is the first time that it has been shown that inhibitors with improved efficacy can be obtained by mutagenesis of PrP sequences at sites associated with prion disease susceptibility.
SCIENTIFIC REPORTS
(2023)
Article
Biochemical Research Methods
Juri Sakata, Toshifumi Tatsumi, Akira Sugiyama, Akihiro Shimizu, Yuya Inagaki, Hiroto Katoh, Takefumi Yamashita, Kazuki Takahashi, Sho Aki, Yudai Kaneko, Takeshi Kawamura, Mai Miura, Masazumi Ishii, Tsuyoshi Osawa, Toshiya Tanaka, Shumpei Ishikawa, Masanobu Tsukagoshi, Michael Chansler, Tatsuhiko Kodama, Motomu Kanai, Hidetoshi Tokuyama, Kenzo Yamatsugu
Summary: This study reports an expression, refolding, and purification method for antibody-mimetic drug conjugate (AMDC). The AMDC uses a variable heavy chain of heavy chain-only antibodies (VHHs) as the targeting protein and non-covalently binds to a potent DNA-alkylating drug. The AMDC shows strong cytotoxic effects on cancer cells.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Yao Wang, Shaoting Weng, Yajie Tang, Sen Lin, Xiayue Liu, Wenhui Zhang, Gang Liu, Boomi Pandi, Yinrong Wu, Lei Ma, Lin Wang
Summary: In this study, a high yield of recombinant CD20 and claudin 18.2 proteins was achieved using an in vitro coupled transcription-translation system. The results showed that rituximab has a high affinity with CD20 protein. This study provides a novel concept for promoting the expression of multi-pass transmembrane proteins and lays the foundation for large-scale industrial production of membrane-associated drug targets.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Xiaobing Chen, Zijuan Zou, Wei Li, Xu Dong, Yi Chen, Yan Lu, Mingyue Zhu, Mengsen Li, Bo Lin
Summary: In this study, the fusion protein ImI-AFP3, composed of alpha-Conotoxin ImI and human alpha fetoprotein domain 3 (AFP3), was found to inhibit the growth and migration of lung cancer cells and showed synergistic effects with the drug gefitinib. These findings suggest that ImI-AFP3 is a promising candidate for the development of anticancer drugs.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Angel Castillo-Corujo, Mirva J. Saaranen, Lloyd W. Ruddock
Summary: This study successfully expressed two Fabs antibodies in the cytoplasm of E. coli using the CyDisCo system, achieving high yields and biological activity under industrially relevant fermentation conditions.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Junling Guo, Zhongyi Cheng, Zhemin Zhou
Summary: This study focused on a halophilic archaeal nitrile hydratase (NHase) and found that it exhibited higher tolerance to substrates and products compared to NHases from other sources. The unique genetic structure of this highly stable archaeal NHase could provide a theoretical foundation for modifying and enhancing the industrial application of NHase.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Yunlong Shen, Ruirui Zhang, Xiaohua Jiang, Jinliang Yang
Summary: The study developed a monoclonal antibody, B1M023, that can bind to LILRB1 with high affinity and block its binding to HLA-G. This antibody can promote the activation and IFN-gamma secretion of T cells, suggesting its potential applications in concomitant diagnosis and tumor immunotherapy.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Jun Jiang, Zhengqiang Jiang, Qiaojuan Yan, Susu Han, Shaoqing Yang
Summary: A novel alginate lyase gene from a marine bacterium showed efficient enzyme activity and stability, producing high conversion ratios of alginate oligosaccharides.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Xianghua Xiong, Yujin Qiu, Jiahao Zheng, Ling Zhou, Qingyang Wang, Jinglun Pang, Weicai Zhang, Huipeng Chen, Gang Liu, Xiaodong Han
Summary: A specific monoclonal antibody ML419 has been found to disrupt the recognition between Botulinum neurotoxin serotype A (BoNT/A) and FGFR3, effectively preventing BoNT/A from entering neurons. In vivo experiments show that ML419 has a strong protective effect, making it a promising candidate for the development of therapeutics against BoNT/A.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Maria Rain Jennings, Soohyon Min, Grace S. Xu, Kassandra Homayuni, Bhavana Suresh, Yusef Amir Haikal, John Blazeck
Summary: This study successfully produced Homo sapiens adenosine deaminase isoform 1 (HsADA1) and its variants through optimizing the recombinant expression process. The D8N variant of HsADA1 was found to be about 30% less active than the wildtype, but it better retained its activity in human serum. Additionally, the study revealed a previously undescribed phenomenon involving albumin that contributed to the increased activity of HsADA1 and the D8N variant in serum.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Oinam Sangita Devi, Senjam Sunil Singh, K. Rana, Sorokhaibam Jibankumar Singh, Wayenbam Sobhachandra Singh
Summary: A new lectin with hemagglutination activity was purified from the rhizome of Xanthosoma violaceum Schott. The lectin showed different reactions towards human red blood cells of different blood groups. It exhibited optimal hemagglutination activity at specific temperature and pH range, and showed good stability.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
